Abstract

A gene cassette for the knock-out of at least on target gene in yeast cells, the cassette comprises: (a) a URA3 gene as a marker gene; (b) at least one gene disruption auxiliary (gda) sequence; and (c) the upstream and downstream sequences of the target gene, wherein the gda sequence is a sequence having at least 300 to 600 consective residues of the nucleotide sequence of SEQ ID NO: 39.

IPC Classes  ?

• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12N 15/60 - Lyases (4)
• C12R 1/74 - Candida tropicalis

Abstract

A emulsifier composition comprises a water-in-oil emulsifier and a fatty salt of an amido amine having the structure R1-C(0)-NH- R2-N(R3)(R4) or the precursor mixture thereof consisting of the corresponding fatty acid and the amido amine. The process for the preparation of the water-in-oil emulsion involves rapidly mixing all the oil and the water phase together by the use of the emulsifier composition. And the water-in-oil emulsion comprising the emulsifier composition is also provided.

IPC Classes  ?

• C11D 3/30 - AminesSubstituted amines
• C11D 3/32 - AmidesSubstituted amides
• C11D 1/722 - Ethers of polyoxyalkylenes having mixed oxyalkylene groups
• C11D 1/82 - Compounds containing silicon
• A61K 8/41 - Amines
• A61K 8/42 - Amides
• A61K 8/894 - Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone modified by a polyoxyalkylene group, e.g. cetyl dimethicone copolyol

Abstract

This invention relates to a process of preparing highly pure, non-yellowing methacrylic acid necessary for high-tech applications requiring long-term color stability in the presence of amines.

IPC Classes  ?

• C07C 51/21 - Preparation of carboxylic acids or their salts, halides, or anhydrides by oxidation with molecular oxygen
• C07C 51/42 - SeparationPurificationStabilisationUse of additives
• C07C 57/04 - Acrylic acidMethacrylic acid
• C07C 57/05 - Preparation by oxidation in the gaseous phase
• C07C 57/055 - Preparation by oxidation in the gaseous phase starting from unsaturated aldehydes
• C07C 57/07 - SeparationPurificationStabilisationUse of additives

Abstract

Provided are a process for preparing precipitated silicas, precipitated silicas and their use as a filler in an elastomer. The process comprises the steps of: preparing initial charge; simultaneously metering silicate and acidifying agent into the initial charge and reacidifying the precipitation suspension to a pH of 2.5 to 6.0, wherein the silicate used has metal oxide of 4wt% ~7wt% and silicon dioxide of 14wt% ~23wt% and the acidifying agent is selected from the group consisting of sulphuric acid of 90wt%~98.5wt%, hydrochloric acid of 34wt%~42.7wt%, nitric acid of 60wt%~68wt%, phosphoric acid of 80wt%~100wt%, carbonic acid or CO2 and sodium hydrogen sulphite or SO2.

IPC Classes  ?

• C01B 33/193 - Preparation of finely divided silica neither in sol nor in gel formAfter-treatment thereof by acidic treatment of silicates of aqueous solutions of silicates
• C01B 33/18 - Preparation of finely divided silica neither in sol nor in gel formAfter-treatment thereof
• C01B 33/12 - SilicaHydrates thereof, e.g. lepidoic silicic acid
• C01B 33/113 - Silicon oxidesHydrates thereof
• C08K 3/36 - Silica
• C09C 1/28 - Compounds of silicon
• C08L 21/00 - Compositions of unspecified rubbers
• B60C 1/00 - Tyres characterised by the chemical composition or the physical arrangement or mixture of the composition