Abstract

Provided herein, inter alia, are methods of making, amplifying, and sequencing tagged nucleic acid complements, compositions including barcoded adapters, and kits useful in obtaining long-range sequence data.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are devices and methods for transfer and analysis of tissue sections using carrier substrates.

IPC Classes  ?

• G01N 1/30 - StainingImpregnating
• A61L 27/28 - Materials for coating prostheses
• A61L 27/52 - Hydrogels or hydrocolloids
• C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6825 - Nucleic acid detection involving sensors
• C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase
• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• G01N 1/06 - Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
• G01N 1/28 - Preparing specimens for investigation
• G01N 1/31 - Apparatus therefor
• G01N 1/36 - Embedding or analogous mounting of samples
• G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides

Abstract

Arrays are an important tool in biomedical research, providing a platform that arranges biological samples and enables high-throughput analyses. Delivering breakthroughs in proteomics, multiplexed immunoassays, and complex genomic analyses, arrays (e.g., microarrays and nanoarrays) can be designed to host thousands, millions, or even billions, of features that are subjected to simultaneous reaction conditions. Disclosed herein, inter alia, are degradable nanoparticles, nanoarrays, and methods of use thereof.

IPC Classes  ?

• C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase
• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are devices, compositions, kits, and methods for interrogating biological samples.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6869 - Methods for sequencing
• G01N 21/64 - FluorescencePhosphorescence

Abstract

Disclosed herein, inter alia, are methods and systems of image analysis useful for rapidly identifying and/or quantifying features.

IPC Classes  ?

• G01N 21/64 - FluorescencePhosphorescence
• G02B 21/08 - Condensers
• G02B 21/16 - Microscopes adapted for ultraviolet illumination
• G02B 21/18 - Arrangements with more than one light-path, e.g. for comparing two specimens
• G02B 21/26 - StagesAdjusting means therefor
• G02B 27/10 - Beam splitting or combining systems
• H04N 23/16 - Optical arrangements associated therewith, e.g. for beam-splitting or for colour correction
• H04N 23/45 - Cameras or camera modules comprising electronic image sensorsControl thereof for generating image signals from two or more image sensors being of different type or operating in different modes, e.g. with a CMOS sensor for moving images in combination with a charge-coupled device [CCD] for still images
• H04N 23/56 - Cameras or camera modules comprising electronic image sensorsControl thereof provided with illuminating means
• H04N 23/84 - Camera processing pipelinesComponents thereof for processing colour signals

Abstract

Disclosed herein, inter alia, are compositions, methods, and kits useful for detecting nucleobase modifications on one or both strands of a double-stranded nucleic acid fragment.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
• C12Q 1/6844 - Nucleic acid amplification reactions

Abstract

Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections.

IPC Classes  ?

• G01N 1/28 - Preparing specimens for investigation

Abstract

Disclosed herein, inter alia, are nucleotide compounds including cleavable moieties and fluorophore moieties, and methods of use thereof.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

Disclosed herein, inter alia, are compositions and methods for detection of biomolecules and biomolecular interactions.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6804 - Nucleic acid analysis using immunogens
• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6855 - Ligating adaptors

Abstract

Provided herein are methods and compositions for improved sequencing techniques using, for example, polymeric particles and/or three-dimensional structures.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/544 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for detecting polynucleotides from and within cells and tissues.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/25 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving enzymes not classifiable in groups
• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6862 - Ligase chain reaction [LCR]

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a cell.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6804 - Nucleic acid analysis using immunogens
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

The present disclosure relates to a flow cell receiver. The flow cell receiver can include at least one platen, having a plurality of ports. The flow cell receiver can include magnets. The flow cell receiver can be configured to automatically align, secure, and retain a flow cell carrier containing a flow cell.

IPC Classes  ?

• B01L 9/00 - Supporting devicesHolding devices

Abstract

Disclosed herein, inter alia, are methods and cleavable compounds that minimize byproduct formation following cleavage in situ.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

Disclosed herein, inter alia, are methods and compositions useful for detecting multiple biological targets.

IPC Classes  ?

• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing a nucleic acid.

IPC Classes  ?

• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• C12Q 1/6869 - Methods for sequencing

Abstract

Disclosed herein, inter alia, are methods and compositions useful for serially detecting multiple biological targets.

IPC Classes  ?

• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

Disclosed herein, inter alia, are methods for the transfer and manipulation of tissue sections using hydrogel substrates.

IPC Classes  ?

• G01N 1/30 - StainingImpregnating
• A61L 27/28 - Materials for coating prostheses
• A61L 27/52 - Hydrogels or hydrocolloids
• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• G01N 1/06 - Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
• G01N 1/28 - Preparing specimens for investigation
• G01N 1/31 - Apparatus therefor
• G01N 1/36 - Embedding or analogous mounting of samples
• G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides

Abstract

Disclosed herein, inter alia, are devices and methods for transfer and analysis of tissue sections using carrier substrates.

IPC Classes  ?

• G01N 1/00 - SamplingPreparing specimens for investigation
• A61L 27/28 - Materials for coating prostheses
• A61L 27/52 - Hydrogels or hydrocolloids
• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• G01N 1/06 - Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
• G01N 1/28 - Preparing specimens for investigation
• G01N 1/30 - StainingImpregnating
• G01N 1/31 - Apparatus therefor
• G01N 1/36 - Embedding or analogous mounting of samples
• G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides

Abstract

Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates and devices.

IPC Classes  ?

• G01N 1/30 - StainingImpregnating
• G01N 1/28 - Preparing specimens for investigation
• G01N 1/31 - Apparatus therefor

Abstract

Disclosed herein, inter alia, are compositions and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates.

IPC Classes  ?

• C12Q 1/6825 - Nucleic acid detection involving sensors
• C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase
• C12Q 1/6844 - Nucleic acid amplification reactions
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances

Abstract

Disclosed herein, inter alia, are complexes, kits, and efficient methods of sequencing two strands of a double stranded polynucleotide.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Provided herein are methods of detecting polynucleotides with a set of barcoded oligonucleotide probes.

IPC Classes  ?

• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/6869 - Methods for sequencing

Abstract

Disclosed herein, inter alia, are compositions and methods for efficient amplification and sequencing of nucleic acid templates.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are methods and compositions useful for detecting multiple biological targets.

IPC Classes  ?

• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

Disclosed herein, inter alia, are imaging systems, devices, and methods of use thereof.

IPC Classes  ?

• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• G01N 21/64 - FluorescencePhosphorescence
• G02B 21/16 - Microscopes adapted for ultraviolet illumination
• H04N 23/67 - Focus control based on electronic image sensor signals
• H04N 23/695 - Control of camera direction for changing a field of view, e.g. pan, tilt or based on tracking of objects

Abstract

Disclosed herein, inter alia, are detectable compounds including a triplet state quencher and the method of use thereof.

IPC Classes  ?

• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• C07D 471/04 - Ortho-condensed systems
• C09B 23/10 - Methine or polymethine dyes, e.g. cyanine dyes characterised by the methine chain containing an even number of CH groups
• C09K 11/06 - Luminescent, e.g. electroluminescent, chemiluminescent, materials containing organic luminescent materials

Abstract

Disclosed herein, inter alia, are compounds and methods of using the same for the modifying a nucleic acid molecule.

IPC Classes  ?

• C07H 19/14 - Pyrrolo-pyrimidine radicals
• C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

Provided herein are methods including alternating series of sequencing cycles and dark extension cycles allowing longer read lengths and addressing disadvantages of traditional nucleic acid sequencing protocols.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing

Abstract

Disclosed herein, inter alia, are compositions and methods for depleting nucleotide impurities in nucleotide solutions.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a tissue sample.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation

Abstract

Disclosed herein, inter alia, are compositions and methods for amplification and sequencing of nucleic acids.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are modified nucleotides and methods of using the same in nucleic acid sequencing reactions.

IPC Classes  ?

• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
• C12Q 1/6825 - Nucleic acid detection involving sensors

Abstract

Disclosed herein, inter alia, are compositions, methods, and kits for sequencing nucleic acid molecules, such as immune receptor transcripts.

IPC Classes  ?

• C12Q 1/6881 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are methods and compositions for amplifying and sequencing a plurality of template nucleic acids.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

Disclosed herein, inter alia, are reducing agents and methods of use thereof.

IPC Classes  ?

• C07F 9/6521 - Six-membered rings

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a tissue sample.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation

Abstract

The present disclosure relates to a flow cell carrier. The flow cell carrier may include a flow cell and a frame. The frame may include a pocket and a handle. The pocket may have at least one spring feature and at least one banking feature. The frame may be configured to retain the flow cell within the pocket such that a maximal surface area of the flow cell is exposed to an optical lens. Related methods and kits are also disclosed.

IPC Classes  ?

• G01N 15/1404 - Handling flow, e.g. hydrodynamic focusing
• G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry

Abstract

Disclosed herein, inter alia, are compositions and methods for amplification and sequencing of nucleic acid molecules including, for example, genetic mutations.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

Disclosed herein, inter alia, are methods and cleavable compounds that minimize byproduct formation following cleavage.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Disclosed herein, inter alia, are therapeutic and theragnostic compounds and methods of use thereof.

IPC Classes  ?

• A61K 31/4745 - QuinolinesIsoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having nitrogen as a ring hetero atom, e.g. phenanthrolines
• A61K 47/68 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
• C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans

Abstract

Disclosed herein, inter alia, are methods and compositions for detecting a plurality of nucleic acids in a sample.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are fluorescent compounds and methods of making and using thereof.

IPC Classes  ?

• C09B 11/24 - Phthaleins containing amino groups
• A61K 49/00 - Preparations for testing in vivo
• C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C12Q 1/6869 - Methods for sequencing

Abstract

Disclosed herein, inter alia, are mutant enzymes, kits, and methods of use thereof.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12Q 1/6844 - Nucleic acid amplification reactions

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a tissue sample.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation

Abstract

Disclosed herein, inter alia, are silicon containing detectable compounds and methods of use thereof. In an aspect is provided a monovalent nucleotide or monovalent nucleoside covalently bound to a monovalent form of a compound described herein (e.g., wherein the R13 moiety of a compound described herein has reacted with a bioconjugate reactive group to form a bioconjugate linker thereby covalently bonding the monovalent compound to the monovalent nucleotide or monovalent nucleoside).

IPC Classes  ?

• C09B 62/36 - Reactive dyes, i.e. dyes which form covalent bonds with the substrates or which polymerise with themselves with the reactive group directly attached to a heterocyclic ring to some other heterocyclic ring
• C09K 11/06 - Luminescent, e.g. electroluminescent, chemiluminescent, materials containing organic luminescent materials
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances

Abstract

Disclosed herein, inter alia, are devices and methods for transfer and analyses of tissue sections using carrier substrates.

IPC Classes  ?

• G01N 1/00 - SamplingPreparing specimens for investigation
• A61L 27/28 - Materials for coating prostheses
• A61L 27/52 - Hydrogels or hydrocolloids
• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• G01N 1/06 - Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
• G01N 1/28 - Preparing specimens for investigation
• G01N 1/30 - StainingImpregnating
• G01N 1/31 - Apparatus therefor
• G01N 1/36 - Embedding or analogous mounting of samples
• G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides

Abstract

Disclosed herein, inter alia, are complexes, kits, and efficient methods of sequencing two strands of a double stranded polynucleotide.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof for sequencing a nucleic acid.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C07F 9/655 - Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
• C07H 19/06 - Pyrimidine radicals
• C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C07H 19/14 - Pyrrolo-pyrimidine radicals
• C07H 19/16 - Purine radicals
• C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Disclosed herein, inter alia, are methods and compounds including cleavable moieties, and methods of use thereof.

IPC Classes  ?

• A61K 31/704 - Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin, digitoxin
• A61K 31/337 - Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
• A61K 31/4745 - QuinolinesIsoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having nitrogen as a ring hetero atom, e.g. phenanthrolines
• A61K 31/5355 - Non-condensed oxazines containing further heterocyclic rings
• A61K 31/7068 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid

Abstract

Disclosed herein, inter alia, are compositions, methods, and kits useful for detecting nucleobase modifications on one or both strands of a double-stranded nucleic acid fragment.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase

Abstract

Disclosed herein, inter alia, are polynucleotide probes, methods, and kits useful for amplifying and detecting target nucleic acids.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

Disclosed herein, inter alia, are oligonucleotides, methods, and kits useful for amplifying and detecting target nucleic acids.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are oligonucleotides, methods, and kits useful for amplifying and detecting targets such as nucleic acids, proteins, and carbohydrates.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are compositions and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates.

IPC Classes  ?

• G01N 1/10 - Devices for withdrawing samples in the liquid or fluent state
• A61L 27/28 - Materials for coating prostheses
• A61L 27/52 - Hydrogels or hydrocolloids
• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• G01N 1/06 - Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
• G01N 1/28 - Preparing specimens for investigation
• G01N 1/30 - StainingImpregnating
• G01N 1/31 - Apparatus therefor
• G01N 1/36 - Embedding or analogous mounting of samples
• G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides

Abstract

Disclosed herein, inter alia, are compositions and methods for efficient amplification and sequencing of nucleic acid templates.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

Disclosed herein, inter alia, are methods and compositions for improved circular polynucleotide amplification and detection.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/682 - Signal amplification

Abstract

Disclosed herein, inter alia, are oligonucleotides, methods, and kits useful for amplifying and detecting targets such as nucleic acids, proteins, and carbohydrates.

IPC Classes  ?

• C12Q 1/682 - Signal amplification
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

Provided herein are modified Archaeal family B polymerases derived from species of the Archaeal microorganism Pyrococcus that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

A connector assembly is configured to fluidly and removably connect a first set of microtubes to a second set of microtubes of a microfluidic device. The connector assembly provides multiple points of contact to the microtubes to provide a secure connection between the connector assembly and the microtubes. A single gasket provides a securely aligned connection between microtubes.

IPC Classes  ?

• F16L 39/00 - Joints or fittings for double-walled or multi-channel pipes or pipe assemblies
• F16L 37/56 - Couplings of the quick-acting type for double-walled or multi-channel pipes

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a cell.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6804 - Nucleic acid analysis using immunogens
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

Pyrococcus that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are compounds, compositions, and methods of using the same for the sequencing of a nucleic acid.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C07H 19/14 - Pyrrolo-pyrimidine radicals
• C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

Disclosed herein, inter alia, are compositions and methods for detecting polynucleotides within a three-dimensional matrix.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are nanoarrays and methods of use thereof.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• C40B 40/06 - Libraries containing nucleotides or polynucleotides, or derivatives thereof

Abstract

Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

Provided herein are methods for sequencing both strands of a double stranded nucleic acid fragment that improves fidelity and accuracy of a sequence determination compared to traditional next generation sequencing methods. Compositions and kits for use in the methods are also provided.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6855 - Ligating adaptors

Abstract

Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Pyrococcus abyssi that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.

IPC Classes  ?

• C12Q 1/6855 - Ligating adaptors
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

Disclosed herein, inter alia, are methods and compositions useful for increasing monoclonal nucleic acid amplification products on a solid support.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6844 - Nucleic acid amplification reactions

Abstract

The present disclosure relates to a flow cell receiver. The flow cell receiver can include at least one platen, having a plurality of ports. The flow cell receiver can include magnets. The flow cell receiver can be configured to automatically align, secure, and retain a flow cell carrier containing a flow cell.

IPC Classes  ?

• B01L 9/00 - Supporting devicesHolding devices

Abstract

Disclosed herein, inter alia, are methods for increasing monoclonal nucleic acid amplification products on a solid support.

IPC Classes  ?

• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

Disclosed herein, inter alia, are compositions, complexes, and methods useful for efficient multiplex assays.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances

Abstract

Disclosed herein, inter alia, are methods for increasing monoclonal nucleic acid amplification products on a solid support.

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

Disclosed herein, inter alia, are methods and systems of image analysis useful for identifying and/or quantifying features in patterns.

IPC Classes  ?

• G06T 7/00 - Image analysis
• G06T 7/11 - Region-based segmentation
• G06T 5/00 - Image enhancement or restoration

Abstract

Disclosed herein, inter alia, are methods and systems of image analysis useful for identifying and/or quantifying features in patterns.

IPC Classes  ?

• G06T 7/00 - Image analysis
• G16B 30/00 - ICT specially adapted for sequence analysis involving nucleotides or amino acids
• G06T 3/00 - Geometric image transformations in the plane of the image
• G06T 7/33 - Determination of transform parameters for the alignment of images, i.e. image registration using feature-based methods

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a cell.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6804 - Nucleic acid analysis using immunogens
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

Disclosed herein, inter alia, are compositions and methods for spatial detection of biomolecular interactions.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation

Abstract

The present disclosure provides improved nucleic acid sequencing-by-synthesis (SBS) methods, related kits and reagents, and systems for performing such methods using such kits and reagents.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• C12Q 1/6867 - Replicase-based amplification, e.g. using Q-beta replicase

Abstract

Disclosed herein, inter alia, are methods and cleavable compounds that minimize byproduct formation following cleavage.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Disclosed herein, inter alia, are degradable nanoparticles, nanoarrays, and methods of use thereof in nucleic acid sequencing.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

The present disclosure relates to a flow cell carrier. The flow cell carrier may include a flow cell and a frame. The frame may include a pocket and a handle. The pocket may have at least one spring feature and at least one banking feature. The frame may be configured to retain the flow cell within the pocket such that a maximal surface area of the flow cell is exposed to an optical lens. Related methods and kits are also disclosed.

IPC Classes  ?

• G01N 15/1404 - Handling flow, e.g. hydrodynamic focusing
• G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry

Abstract

A device comprising: a sample stage configured to be coupled to a microplate receiver; a microplate receiver configured to be coupled to a microplate; at least one heating element thermally coupled to the microplate receiver; a fluidics dispenser configured to dispense one or more reagents into the microplate; and an imaging system configured to detect one or more features in the microplate; and a structure physically coupled to the sample stage, the heating element, the fluidics dispenser, and the imaging system.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation
• C12Q 1/6869 - Methods for sequencing
• G01N 21/64 - FluorescencePhosphorescence

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a sample comprising a cell.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6841 - In situ hybridisation

Abstract

Disclosed herein, inter alia, are compositions and methods for amplification of nucleic acid templates, including hybridizing a linear polynucleotide to an immobilized primer on a surface, circularizing the linear polynucleotide to form a circular polynucleotide, and extending the primer with a polymerase.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a sample comprising a cell.

IPC Classes  ?

• C12Q 1/6841 - In situ hybridisation

Abstract

Disclosed herein, inter alia, are compositions and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates.

IPC Classes  ?

• A61L 27/52 - Hydrogels or hydrocolloids
• A61L 27/28 - Materials for coating prostheses

Abstract

Disclosed herein, inter alia, are compositions and methods for detecting polynucleotides within a three-dimensional polymer matrix.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

Disclosed herein, inter alia, are methods of making, amplifying, and sequencing compositions, and kits useful in obtaining sequencing data.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]