Arrays are an important tool in biomedical research, providing a platform that arranges biological samples and enables high-throughput analyses. Delivering breakthroughs in proteomics, multiplexed immunoassays, and complex genomic analyses, arrays (e.g., microarrays and nanoarrays) can be designed to host thousands, millions, or even billions, of features that are subjected to simultaneous reaction conditions. Disclosed herein, inter alia, are degradable nanoparticles, nanoarrays, and methods of use thereof.
H04N 23/16 - Optical arrangements associated therewith, e.g. for beam-splitting or for colour correction
H04N 23/45 - Cameras or camera modules comprising electronic image sensorsControl thereof for generating image signals from two or more image sensors being of different type or operating in different modes, e.g. with a CMOS sensor for moving images in combination with a charge-coupled device [CCD] for still images
H04N 23/56 - Cameras or camera modules comprising electronic image sensorsControl thereof provided with illuminating means
H04N 23/84 - Camera processing pipelinesComponents thereof for processing colour signals
4.
NUCLEIC ACID AMPLIFICATION AND METHYLATION PATTERN RETENTION
Disclosed herein, inter alia, are compositions, methods, and kits useful for detecting nucleobase modifications on one or both strands of a double-stranded nucleic acid fragment.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections.
Provided herein are methods and compositions for improved sequencing techniques using, for example, polymeric particles and/or three-dimensional structures.
G01N 33/544 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
9.
METHODS FOR DETECTING POLYNUCLEOTIDE SEQUENCES IN SITU
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
C12Q 1/25 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving enzymes not classifiable in groups
The present disclosure relates to a flow cell receiver. The flow cell receiver can include at least one platen, having a plurality of ports. The flow cell receiver can include magnets. The flow cell receiver can be configured to automatically align, secure, and retain a flow cell carrier containing a flow cell.
G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G01N 1/36 - Embedding or analogous mounting of samples
G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
20.
Device for tissue section immobilization and retention
G01N 1/36 - Embedding or analogous mounting of samples
G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
21.
METHODS AND DEVICES FOR MANIPULATING BIOLOGICAL SAMPLES
Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates and devices.
Disclosed herein, inter alia, are compositions and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates.
C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase
G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
Provided herein are methods including alternating series of sequencing cycles and dark extension cycles allowing longer read lengths and addressing disadvantages of traditional nucleic acid sequencing protocols.
C12Q 1/6881 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
The present disclosure relates to a flow cell carrier. The flow cell carrier may include a flow cell and a frame. The frame may include a pocket and a handle. The pocket may have at least one spring feature and at least one banking feature. The frame may be configured to retain the flow cell within the pocket such that a maximal surface area of the flow cell is exposed to an optical lens. Related methods and kits are also disclosed.
G01N 15/1404 - Handling flow, e.g. hydrodynamic focusing
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
Disclosed herein, inter alia, are compositions and methods for amplification and sequencing of nucleic acid molecules including, for example, genetic mutations.
A61K 31/4745 - QuinolinesIsoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having nitrogen as a ring hetero atom, e.g. phenanthrolines
A61K 47/68 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
Disclosed herein, inter alia, are silicon containing detectable compounds and methods of use thereof. In an aspect is provided a monovalent nucleotide or monovalent nucleoside covalently bound to a monovalent form of a compound described herein (e.g., wherein the R13 moiety of a compound described herein has reacted with a bioconjugate reactive group to form a bioconjugate linker thereby covalently bonding the monovalent compound to the monovalent nucleotide or monovalent nucleoside).
C09B 62/36 - Reactive dyes, i.e. dyes which form covalent bonds with the substrates or which polymerise with themselves with the reactive group directly attached to a heterocyclic ring to some other heterocyclic ring
G01N 1/36 - Embedding or analogous mounting of samples
G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
54.
Methods of amplifying circular polynucleotides in situ
C07F 9/655 - Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
Disclosed herein, inter alia, are compositions, methods, and kits useful for detecting nucleobase modifications on one or both strands of a double-stranded nucleic acid fragment.
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
A61K 31/704 - Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin, digitoxin
A61K 31/337 - Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
A61K 31/4745 - QuinolinesIsoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having nitrogen as a ring hetero atom, e.g. phenanthrolines
A61K 31/5355 - Non-condensed oxazines containing further heterocyclic rings
A61K 31/7068 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid
58.
METHODS AND DEVICES FOR MANIPULATING BIOLOGICAL SAMPLES
inter aliainter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates and devices.
Disclosed herein, inter alia, are oligonucleotides, methods, and kits useful for amplifying and detecting targets such as nucleic acids, proteins, and carbohydrates.
Disclosed herein, inter alia, are compositions and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates.
G01N 1/36 - Embedding or analogous mounting of samples
G01N 23/2251 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or by measuring secondary emission from the material using electron or ion microprobes using incident electron beams, e.g. scanning electron microscopy [SEM]
G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
63.
MULTIPLEXED TARGETED AMPLIFICATION OF POLYNUCLEOTIDES
Disclosed herein, inter alia, are oligonucleotides, methods, and kits useful for amplifying and detecting targets such as nucleic acids, proteins, and carbohydrates.
Provided herein are modified Archaeal family B polymerases derived from species of the Archaeal microorganism Pyrococcus that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.
A connector assembly is configured to fluidly and removably connect a first set of microtubes to a second set of microtubes of a microfluidic device. The connector assembly provides multiple points of contact to the microtubes to provide a secure connection between the connector assembly and the microtubes. A single gasket provides a securely aligned connection between microtubes.
Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide.
Provided herein are methods for sequencing both strands of a double stranded nucleic acid fragment that improves fidelity and accuracy of a sequence determination compared to traditional next generation sequencing methods. Compositions and kits for use in the methods are also provided.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
Goods & Services
Kits comprised of assays, buffers, enzymes, nucleotides,
proteins, reagents, nucleic acids, oligonucleotides,
chemical preparations, and biological compounds for use in
connection with cellular analysis, tissue analysis, protein
analysis, lipid analysis, carbohydrate analysis, genomic
testing, genotyping, sequencing, and analysis for scientific
and research purposes and for clinical or medical laboratory
use. Kits comprised of assays, reagents, nucleic acid, proteins,
oligonucleotides, chemical preparations, and biological
compounds for use in connection with cellular analysis,
tissue analysis, protein analysis, lipid analysis,
carbohydrate analysis, genomic testing, sequencing, and
analysis for medical diagnostic, clinical medical and
medical purposes. Laboratory devices for imaging cells and tissues for
detecting, measuring, testing, and analyzing proteins,
biomolecules, cellular components, nucleic acid molecules,
and genetic sequences; downloadable computer software for
use in testing, sequencing, and analysis of biomolecules,
DNA, and genomes, sold as an integral component of
laboratory apparatuses.
Disclosed herein, inter alia, are mutant DNA polymerase enzymes, kits, and methods of use thereof. Specifically, the mutant DNA polymerases exhibit point mutations at amino acid residues such as L216, preferably L216P. The methods detail incorporation of variant nucleotides into the polymerase sequences and amplification of template polynucleotides by the resulting mutant polymerases.
Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Pyrococcus abyssi that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.
Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.
The present disclosure relates to a flow cell receiver. The flow cell receiver can include at least one platen, having a plurality of ports. The flow cell receiver can include magnets. The flow cell receiver can be configured to automatically align, secure, and retain a flow cell carrier containing a flow cell.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
84.
METHODS AND COMPOSITIONS FOR AMPLIFYING POLYNUCLEOTIDES
The present disclosure provides improved nucleic acid sequencing-by-synthesis (SBS) methods, related kits and reagents, and systems for performing such methods using such kits and reagents.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12Q 1/6867 - Replicase-based amplification, e.g. using Q-beta replicase
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Kits comprising of assays, buffers, enzymes, nucleotides,
reagents, nucleic acids, oligonucleotides, chemical
preparations, solid support substrates, and biological
compounds for use in connection with sample preparation,
cellular analysis, protein and proteomic analysis, genomic
expression, genomic testing, genotyping, sequencing, and
analysis for scientific and research purposes. Scientific testing in the field of analysis, detecting and
sequencing of biomolecules, DNA, and genomes for research
purposes; providing medical research and scientific research
and information in the field of testing, analysis, gene
expression, cellular analysis, protein and proteomic
analysis, and sequencing of biomolecules, DNA, RNA and
genomes.
The present disclosure relates to a flow cell carrier. The flow cell carrier may include a flow cell and a frame. The frame may include a pocket and a handle. The pocket may have at least one spring feature and at least one banking feature. The frame may be configured to retain the flow cell within the pocket such that a maximal surface area of the flow cell is exposed to an optical lens. Related methods and kits are also disclosed.
G01N 15/1404 - Handling flow, e.g. hydrodynamic focusing
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
98.
Multiomic analysis device and methods of use thereof
A device comprising: a sample stage configured to be coupled to a microplate receiver; a microplate receiver configured to be coupled to a microplate; at least one heating element thermally coupled to the microplate receiver; a fluidics dispenser configured to dispense one or more reagents into the microplate; and an imaging system configured to detect one or more features in the microplate; and a structure physically coupled to the sample stage, the heating element, the fluidics dispenser, and the imaging system.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
Goods & Services
Kits comprised of assays, buffers, enzymes, nucleotides, proteins, reagents, nucleic acids, oligonucleotides, chemical preparations, and biological compounds for use in connection with cellular analysis, tissue analysis, protein analysis, lipid analysis, carbohydrate analysis, genomic testing, genotyping, sequencing, and analysis for scientific and research purposes and for clinical or medical laboratory use Kits comprised of assays, reagents, nucleic acid, proteins, oligonucleotides, chemical preparations, and biological compounds for use in connection with cellular analysis, tissue analysis, protein analysis, lipid analysis, carbohydrate analysis, genomic testing, sequencing, and analysis for medical diagnostic, clinical medical and medical purposes Laboratory devices for imaging cells and tissues for detecting, measuring, testing, and analyzing proteins, biomolecules, cellular components, nucleic acid molecules, and genetic sequences; Downloadable computer software for use in testing, sequencing, and analysis of biomolecules, DNA, and genomes, sold as an integral component of laboratory apparatuses
