Candida species capable of producing sophorolactone, in absence of an oily substrate until a stationary growth phase is obtained, —cultivating said pre-cultivated cells in an aqueous medium in the presence of at least one fermentable sugar and substrate; the reaction mixture of sugar, substrate and pre-cultivated cells being present in an amount and conditions such that the cells metabolize the sugar and substrate thereby forming sophorolactone and fatty acid, —continuously feeding said substrate to said cells thereby suppressing the formation of fatty acid and keeping fatty acid levels in the reaction mixture below 10 g/l, resulting in the crystallization of at least part of the sophorolactone present in the reaction mixture, —warming the reaction mixture to a temperature between 60° C. and 90° C., thereby melting the sophorolactone crystals, —allowing the molten sophorolactone to settle and to provide a crude sophorolactone composition, and —removing the crude sophorolactone composition from the remainder of the reaction mixture without use of an organic solvent.
C07H 15/10 - Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of a saccharide radical containing unsaturated carbon-to-carbon bonds
C12P 19/44 - Preparation of O-glycosides, e.g. glucosides
C12N 15/52 - Genes encoding for enzymes or proenzymes
C12N 15/01 - Preparation of mutants without inserting foreign genetic material thereinScreening processes therefor
C07H 15/04 - Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of a saccharide radical
C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
The present invention relates to a surfactant composition, consisting of 70 wt %-99.9 wt % sophorolactone, 0-1 wt % sophorolipid acid, less than 0.1% residual substrate, and remainder water. The invention further relates to products comprising this composition or hydrolysates thereof. The invention also relates to uses of these products and hydrolysates.
A01N 25/30 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
A01N 43/90 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
A61K 47/26 - Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharidesDerivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
A01N 49/00 - Biocides, pest repellants or attractants, or plant growth regulators containing compounds containing the group wherein m+n≥1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group wherein A means a carbon atom or Y, n ≥ 0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
The present Invention relates to a coated detergent tablet comprising a tablet core surrounded by a film coating, wherein the tablet core comprises a moisture- sensitive detergent composition; the film coating comprises a sugar or sugar derivative selected from sorbitol, mannltol, maltltol, xylltol, lactltol, isomalt, erytrhitol, lactose, sophorolactone or mixtures thereof; and wherein the film coating protects the tablet core against an increase in weight, expressed in relation to the tablet, of > 7% when exposed to 70% relative air humidity for 8 weeks at 25°C In addition, the invention provides a method to fabricate the detergent tablets. Moreover, the invention relates to the use of sugar alcohols as a coating material for detergent tablets.
The present invention relates to a surfactant composition, consisting of 70 wt%-99.9 wt% sophorolactone, 0-1 wt% sophorolipid acid, less than 0.1 % residual substrate, and remainder water. The invention further relates to products comprising this composition or hydrolysates thereof. The invention also relates to uses of these products and hydrolysates.
The present invention relates to a process for selectively producing sophorolactone without use of organic solvent, comprising the steps of: -pre-cultivating cells of a Candida species capable of producing sophorolactone, in absence of an oily substrate until a stationary growth phase is obtained, -cultivating said pre-cultivated cells in an aqueous medium in the presence of at least one fermentable sugar and substrate; the reaction mixture of sugar, substrate and pre-cultivated cells being present in an amount and conditions such that the cells metabolize the sugar and substrate thereby forming sophorolactone and fatty acid, -continuously feeding said substrate to said cells thereby suppressing the formation of fatty acid and keeping fatty acid levels in the reaction mixture below 10 g/l, resulting in the crystallization of at least part of the sophorolactone present in the reaction mixture, -warming the reaction mixture to a temperature between 60°C and 90°C, thereby melting the sophorolactone crystals, -allowing the molten sophorolactone to settle and to provide a crude sophorolactone composition, and -removing the crude sophorolactone composition from the remainder of the reaction mixture without use of an organic solvent.
C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
The present invention relates to a process, comprising the steps of: -pre-cultivating cells of a Candida species capable of producing sophorolactone, until a stationary growth phase is obtained, -cultivating said pre-cultivated cells in the presence of at least one fermentable sugar and substrate; the reaction mixture of sugar, substrate and pre-cultivated cells being present in an amount and conditions such that the cells metabolize the sugar and substrate thereby forming sophorolactone and fatty acid, -suppressing the formation of fatty acid in the reaction mixture below a predetermined level thereby crystallizing at least part of the sophorolactone present in the reaction mixture, -warming the reaction mixture to a temperature between 60°C and 90°C thereby melting the sophorolactone crystals, -allowing the molten sophorolactone to settle and to provide a crude sophorolactone composition,and -removing the crude sophorolactone composition from the remainder of the reaction mixture.
C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
C. bombicola is disrupted with the purpose of blocking the beta-oxidation pathway in the strain. Fermentation of a such strain having such disrupted genes on primary or secondary alcohols or diols, preferably on primary alcohols produces short chained glycolipids in high yield and purity.
C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
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