Goods & Services

Tensio-active agents; protein [raw material]; collagen for industrial purposes; emulsifiers; cultures of microorganisms, other than for medical and veterinary use; chemical preparations for scientific purposes, other than for medical or veterinary use; proteins for the food industry; proteins for use in the manufacture of food supplements; preservatives for pharmaceutical preparations; gelatine for industrial purposes. Medical apparatus and instruments; artificial teeth; dental apparatus and instruments; dental burs; surgical sponges; surgical implants comprised of artificial materials; artificial eyes; artificial skin for surgical purposes; suture materials; thread, surgical.

Goods & Services

Tensio-active agents, namely, non-ionic surfactants as raw materials for use in the manufacture of cosmetics; Protein in raw material form for scientific and medical research; collagen for industrial purposes; emulsifiers for use in for industrial manufacturing; cultures of microorganisms, other than for medical and veterinary use; chemical preparations for scientific purposes, other than for medical or veterinary use; proteins for the food industry; proteins for use in the manufacture of food supplements; preservatives for pharmaceutical preparations, namely, chemical preparations for preserving foodstuffs; gelatine for industrial purposes Medical apparatus and instruments, namely, devices for repairing damaged skin; artificial teeth; dental apparatus and instruments, namely, artificial teeth; dental burs; surgical sponges; surgical implants comprised of artificial materials; artificial eyes; artificial skin for surgical purposes; suture materials; thread, surgical

Goods & Services

Cleansing milk for toilet purposes; shampoo; facial cleanser; oils for cleaning purposes; essential oils; beauty masks; cosmetics; collagen preparations for cosmetic purposes; anti-wrinkle cream; dentifrices.

Goods & Services

Cleansing milk for toilet purposes; shampoo; facial cleanser; oils for cleaning purposes; essential oils; beauty masks; cosmetics; collagen preparations for cosmetic purposes; anti-wrinkle cream; dentifrices

Goods & Services

Facial cleanser; oils for cleaning purposes; shampoo; cleansing milk for toilet purposes; essential oils; beauty masks; cosmetics; anti-wrinkle cream; dentifrices; collagen preparations for cosmetic purposes.

Goods & Services

Protein [raw material]; collagen for industrial purposes; tensio-active agents; emulsifiers; chemical preparations for scientific purposes, other than for medical or veterinary use; cultures of microorganisms, other than for medical and veterinary use; preservatives for use in the pharmaceutical industry; proteins for use in the manufacture of food supplements; proteins for the food industry; gelatine for industrial purposes. Nutraceutical preparations for therapeutic or medical purposes; protein dietary supplements; collagen for medical purposes; drugs for medical purposes; gelatine for medical purposes; peptones for pharmaceutical purposes; adjuvants for medical purposes; crude drug; albuminous foodstuffs for medical purposes; teeth filling material.

Goods & Services

Protein [raw material]; collagen for industrial purposes; tensio-active agents; emulsifiers; chemical preparations for scientific purposes, other than for medical or veterinary use; cultures of microorganisms, other than for medical and veterinary use; preservatives for pharmaceutical preparations; proteins for use in the manufacture of food supplements; proteins for the food industry; gelatine for industrial purposes. Nutraceutical preparations for therapeutic or medical purposes; protein dietary supplements; collagen for medical purposes; drugs for medical purposes; gelatine for medical purposes; peptones for pharmaceutical purposes; adjuvants for medical purposes; crude drug; albuminous foodstuffs for medical purposes; teeth filling material.

Goods & Services

Protein [raw material]; tensio-active agents; collagen for industrial purposes; emulsifiers; chemical preparations for scientific purposes, other than for medical or veterinary use; preservatives for pharmaceutical preparations; proteins for use in the manufacture of food supplements; proteins for the food industry; gelatine for industrial purposes; cultures of microorganisms, other than for medical and veterinary use.

Goods & Services

Protein in raw material form for use in the manufacture of cosmetics; collagen for industrial purposes; tensio-active agents for use in the manufacture of cosmetics products; emulsifiers for use in the manufacture of cosmetics products; chemical preparations for scientific purposes, other than for medical or veterinary use; cultures of microorganisms, other than for medical and veterinary use; preservatives for pharmaceutical preparations; proteins for use in the manufacture of food supplements; proteins for the food industry; gelatine for industrial purposes Nutraceutical preparations for therapeutic or medical purposes; protein dietary supplements; collagen for medical purposes; drugs for medical purposes, namely, health care medicine for the treatment of gastrointestinal diseases; gelatine for medical purposes; peptones for pharmaceutical purposes for treatment of skin diseases; adjuvants for medical purposes; crude drugs being medicated cosmetics; albuminous foodstuffs for medical purposes; teeth filling material

Goods & Services

Protein in raw material form for use in the manufacture of cosmetics; tensio-active agents for use in the manufacture of cosmetics products; collagen for industrial purposes; emulsifiers for use in the manufacture of cosmetics products; chemical preparations for scientific purposes, other than for medical or veterinary use; preservatives for pharmaceutical preparations; proteins for use in the manufacture of food supplements; proteins for the food industry; gelatine for industrial purposes; cultures of microorganisms, other than for medical and veterinary use

Abstract

Pichia pastoris is used as a fungal strain. The process comprises performing primary seed culturing to reach a fungal concentration of 20±2 g/L; then performing secondary seed culturing to reach a fungal concentration of 120±10 g/L; next proceeding to a glycerol culturing stage, wherein the amount of glycerol added in the glycerol culturing stage is 60-70 g/L; and then proceeding to a methanol-induced stage for 120±8 h after the dissolved oxygen quickly reaches a relatively stable state, to complete the fermentation process. In the present invention, a glycerol fed-batch addition stage in existing processes is omitted, and the process proceeds to a next stage as soon as glycerol is completely consumed, with no need to prepare sterilized glycerol for fluidic addition. As such, the glycerin sterilizer is omitted, the consumption of energy and resource and the waste of glycerin are reduced. Moreover, the fungal concentration has no need to be monitored, thus reducing the probability of errors, and lowering the fermentation failure caused by technical errors. Therefore, the present process is more suitable for large-scale industrial production, and brings convenience and great economic value for the industrial production of the recombinant protein.

IPC Classes  ?

• C08L 89/00 - Compositions of proteinsCompositions of derivatives thereof
• C12P 21/00 - Preparation of peptides or proteins
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• C12N 1/16 - YeastsCulture media therefor
• C12R 1/84 - Pichia

Goods & Services

Protein in raw material form for use in the manufacture of cosmetics; collagen for industrial purposes; tensio-active agents for use in the manufacture of cosmetics products; emulsifiers for use in the manufacture of cosmetics products; chemical preparations for scientific purposes, other than for medical or veterinary use; cultures of microorganisms, other than for medical and veterinary use; preservatives for pharmaceutical preparations; proteins for use in the manufacture of food supplements; proteins for the food industry; gelatine for industrial purposes Nutraceutical preparations for therapeutic or medical purposes; protein dietary supplements; collagen for medical purposes; drugs for medical purposes, namely, health care medicine for the treatment of gastrointestinal diseases; gelatine for medical purposes; peptones for pharmaceutical purposes for treatment of skin diseases; adjuvants for medical purposes; crude drugs being medicated cosmetics; albuminous foodstuffs for medical purposes; teeth filling material

Abstract

The present invention provides a fermentation process for improving the production level of a recombinant human collagen. The process comprises inoculating a sterilized fermentation medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hrs, adding methanol to induce the expression, upon which sodium pyruvate is added to the fermentation medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L. In the fermentation process of the present invention, the sodium pyruvate is added in the methanol-induced expression stage, so that the biosynthesis rate of the recombinant human collagen is improved; and a continuous feed-batch mode is adopted, so that the biosynthesis rate of the recombinant human collagen is further improved, and the fermentation time is shortened. Meanwhile, the expression level of the recombinant human collagen is increased, the fermentation level is increased by more than 20%, and the production cost is reduced. The fermentation process is especially suitable for industrialized mass production of recombinant human collagen, and is of huge practical application value in industrial production.

IPC Classes  ?

• C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• C12N 1/16 - YeastsCulture media therefor
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12R 1/84 - Pichia

Goods & Services

Agar-agar; Casein for industrial purposes; Collagen for industrial purposes; Gluten for industrial purposes; Pectin for industrial purposes; Protein for industrial use; Protein in raw material form for scientific and medical research; Proteins for the food industry; Proteins for use in manufacture; Chemical reagents, other than for medical or veterinary purpose

Goods & Services

Agar-agar; Casein for industrial purposes; Collagen for industrial purposes; Gluten for industrial purposes; Pectin for industrial purposes; Protein for industrial use; Protein in raw material form for scientific and medical research; Chemical reagents, other than for medical or veterinary purpose; Proteins for use in manufacture; Proteins for the food industry

Abstract

Provided is a fermentation technique with a Pichia yeast expressing a recombinant protein. The fermentation technique uses Pichia pastoris as the fungal strain. The method comprises: performing primary seed culturing to reach a fungal concentration of 20±2 g/L, then switching to secondary seed culturing to reach a concentration of 120±2 g/L, then switching to a glycerol culturing phase, wherein the amount of glycerol added for the glycerol culturing phase is 60-70 g/L, then waiting for dissolved oxygen to reach a relatively stable state to initiate a methanol induction phase, and performing induction for 120±8 h to complete the fermentation process.

IPC Classes  ?

• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis
• C12R 1/84 - Pichia

Abstract

The present invention provides a fermentation technique to improve the production level of a recombinant human collagen. First inoculating a sterilized fermentation culture medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hours before adding methanol to induce the expression, and adding sodium pyruvate into the culture medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L.

IPC Classes  ?

• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12R 1/84 - Pichia
• C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]