Abstract

The invention provides sustained delivery of nucleic acids from hydrophobic/organic media, formulations and biomaterial depots.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
• A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit

Abstract

Integral membrane proteins capable of transporting melatonin and other indole-derivatives across biological membranes, and uses thereof.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/88 - Lyases (4.)
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• C12P 17/10 - Nitrogen as only ring hetero atom

Abstract

Bioink comprising modified fibers of MFC with a diameter of about 100-400 nm.

IPC Classes  ?

• A61L 27/38 - Animal cells
• A61L 27/20 - Polysaccharides
• A61L 27/22 - Polypeptides or derivatives thereof
• A61L 27/26 - Mixtures of macromolecular materials
• B33Y 70/00 - Materials specially adapted for additive manufacturing
• C08L 1/02 - CelluloseModified cellulose
• C08L 89/00 - Compositions of proteinsCompositions of derivatives thereof

Abstract

The application of geocatalysts in controlling CO2 capture processes in situ as well as in a reaction vessel, chamber or tower is described. More specifically, a process for controlling the reaction of a CO2-containing gas stream with one or more starting materials selected from waste building material, mineral, rock, sand, amorphous material or combinations thereof is disclosed: wherein said starting material comprises Si and/or Al, and one or more divalent cation(s), water, and one or more organic compound(s) to control the reaction: thereby providing a product comprising: at least one carbonate mineral and preferably colloidal silica.

IPC Classes  ?

• B01D 53/62 - Carbon oxides

Abstract

The present invention concerns a method for site-selective modification of a target protein or peptide by use of an acylation tag comprising a single lysine residue and at least three histidine residues. Upon contact with an acylating reagent, the target protein or peptide becomes modified at the ε-amine of the lysine residue of the acylation tag.

IPC Classes  ?

• C07K 1/107 - General processes for the preparation of peptides by chemical modification of precursor peptides
• C07K 1/13 - Labelling of peptides

Abstract

The invention provides a microbial host cell for enhanced recombinant expression of a target protein, said host cell comprising a mutant RNase E enzyme to be coexpressed with a target gene of interest. The invention further provides a method of enhancing recombinant protein expression using said microbial host cell. The method is particularly useful for the expression of proteins that are otherwise difficult to express in traditional expression systems, such as proteins which are toxic to the host cell. The invention further provides an auxiliary plasmid comprising a rne* gene encoding a mutant RNase E enzyme and a LysS gene encoding T7 lysozyme.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C07K 14/245 - Escherichia (G)
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 9/80 - Hydrolases (3.) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli

Abstract

A system for waste gas treatment is provided. The system for waste gas treatment comprises a photochemical reactor comprising an ultraviolet source. The photochemical reactor is configured to receive a waste gas and to break down, by using the ultraviolet source, the waste gas into one or more primary gaseous by-products. The system for waste gas treatment comprises an absorption reactor. The absorption reactor is connected in series with the photochemical reactor. The absorption reactor is configured to collect and/or convert the one or more primary gaseous by-products into one or more secondary by-products by the use of a liquid containing calcium ions.

IPC Classes  ?

• B01D 53/75 - Multi-step processes
• B01D 53/14 - Separation of gases or vapoursRecovering vapours of volatile solvents from gasesChemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by absorption
• B01D 53/56 - Nitrogen oxides
• B01D 53/70 - Organic halogen compounds
• B01D 53/78 - Liquid phase processes with gas-liquid contact

Abstract

The present invention relates to an enzyme exhibiting histidine N-methyltransferase activity, a microbial cell comprising a gene encoding such enzyme, and use of this enzyme for producing a target peptide or protein having a methylated N-terminal histidine residue.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Abstract

The present invention relates to a process for producing a isotopically labelled organic compound or a mixture of such compounds in a gas-fed zero-gap electrolyser device.

IPC Classes  ?

• C25B 9/23 - Cells comprising dimensionally-stable non-movable electrodesAssemblies of constructional parts thereof with diaphragms comprising ion-exchange membranes in or on which electrode material is embedded
• C25B 3/03 - Acyclic or carbocyclic hydrocarbons
• C25B 3/25 - Reduction
• C25B 3/26 - Reduction of carbon dioxide
• C25B 11/032 - Gas diffusion electrodes
• C25B 11/075 - Electrodes formed of electrocatalysts on a substrate or carrier characterised by the electrocatalysts material consisting of a single catalytic element or catalytic compound
• C25B 15/00 - Operating or servicing cells

Abstract

The present disclosure relates to methods for production of phosphorylated casein, phosphorylated phosphomimetic casein and phosphomimetic casein, as well as the use of such caseins.

IPC Classes  ?

• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12R 1/19 - Escherichia coli

Abstract

Disclosed is a method for obtaining a transverse phase gradient of a wave field from at least a first and a second wavefield intensity map comprising the steps of: capturing at a first incoherent tilt aberration said first wave field intensity map of a target at a first degree of incoherent tilt aberration, using a filter positioned between a source of electromagnetic radiation and an electromagnetic radiation detector capturing said first wave field intensity map: capturing at a second and different incoherent tilt aberration said second wave field intensity map of said target at a second degree of incoherent tilt aberration, using a filter positioned between a source of electromagnetic radiation and an electromagnetic radiation detector capturing said second wave field intensity map: determining said transverse phase gradient on the basis of at least a difference of logarithms of wavefield intensity maps divided by the magnitude of the difference between said first incoherent tilt aberration and said second incoherent tilt aberration. An imaging system, a computer program product and a use of the method is further disclosed.

IPC Classes  ?

• H04N 23/951 - Computational photography systems, e.g. light-field imaging systems by using two or more images to influence resolution, frame rate or aspect ratio
• G01J 9/00 - Measuring optical phase differenceDetermining degree of coherenceMeasuring optical wavelength
• G02B 21/00 - Microscopes
• G02B 21/14 - Condensers affording illumination for phase-contrast observation
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• H04N 23/957 - Light-field or plenoptic cameras or camera modules

Abstract

The present invention relates to engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems and corresponding guide RNAs that target specific nucleotide sequences at certain gene loci in the human genome. Also provided are methods of targeting, editing, and/or modifying of the human genes using the engineered CRISPR systems, and compositions and cells comprising the engineered CRISPR systems.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 9/22 - Ribonucleases
• C12N 15/90 - Stable introduction of foreign DNA into chromosome

Abstract

The present invention relates to a composition comprising a genetically engineered non-pathogenic microorganism expressing one or more polypeptide comprising polypeptide hormones. The composition is preferably for use in the treatment of non-alcoholic fat liver disease (NAFLD).

IPC Classes  ?

• A61K 35/744 - Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
• A61K 38/18 - Growth factorsGrowth regulators
• A61K 38/30 - Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
• A61P 1/16 - Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• C07K 14/50 - Fibroblast growth factor [FGF]
• C07K 14/65 - Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2

Abstract

The present disclosure provides a system and a method for cleaning of gas.

IPC Classes  ?

• C25B 1/46 - Simultaneous production of alkali metal hydroxides and chlorine, oxyacids or salts of chlorine, e.g. by chlor-alkali electrolysis in diaphragm cells
• B01D 53/14 - Separation of gases or vapoursRecovering vapours of volatile solvents from gasesChemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by absorption
• B01D 53/52 - Hydrogen sulfide
• B01D 53/75 - Multi-step processes
• B01D 53/78 - Liquid phase processes with gas-liquid contact
• C25B 15/08 - Supplying or removing reactants or electrolytesRegeneration of electrolytes

Abstract

The present disclosure relates to a method for generating a synthetic promoter and/or terminator, the method comprising: obtaining gene sequences, such as functional sequences and/or coding sequences, of genomes of multiple closely related species; identifying activity regulating features, by calculating a correlation coefficient between parameters of the statistical model and features scores of the FMS, and recognizing said activity regulating features as model features of which said correlation coefficient is above a predetermined threshold, such as 0.5, optimizing, for each HMM, the starting consensus sequence, by, for each activity regulating feature, calculating the gene statistics upon inserting/replacing the activity regulating feature into the starting consensus sequence or a partially optimized consensus sequence, and where said insertion/replacement leads to an improvement in the gene statistics, such as an increase by a predetermined threshold, accepting said insertion/replacement, otherwise said insertion/replacement is discarded.

IPC Classes  ?

• G16B 30/10 - Sequence alignmentHomology search
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding

Abstract

In certain embodiments a light therapy system (e.g., phototherapy device), such as for treatment of Alzheimer's disease, depression, dementia, short-term memory, or for improved learning, improved athletic performance or improved cognitive performance, is provided where the light system comprises a blue light source that operates at a frequency in the range from 20 to 50 Hz (preferably around 40 Hz), whereby the system enables retinal ganglion cells of a human to be exposed in order to stimulate brain waves (gamma oscillations in the human's brain).

IPC Classes  ?

• A61N 5/06 - Radiation therapy using light

Abstract

Provided are thermophilic bacteria comprising an expression control system comprising a Tet repressor protein, use of such an expression system to control the expression of a gene of interest in thermophilic bacteria by temperature regulation and methods for producing a gene product of interest in thermophilic bacteria by using this system by temperature regulation.

IPC Classes  ?

• C12P 21/00 - Preparation of peptides or proteins
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Abstract

A terahertz (THz) cross-correlation device for material characterization of a sample comprising an optical source for outputting a continuous-wave (CW) optical signal: THz antennas providing a THz transmitter and a THz receiver optically coupled to the optical source, and an optical delay setup configured to adjust a synchronization of the THz receiver to the THz transmitter by the CW optical signal. The optical source is configured to provide a CW optical signal having an at least substantially continuous broadband spectrum and the optical paths for the CW signal are provided by optical waveguides such as optical fibres or integrated waveguides. The optical delay setup comprises a double-pass polarization-conserving fibre stretcher comprising a circulator, a fibre stretcher, and a Faraday mirror; and/or a variable solid state optical delay (SSOD).

IPC Classes  ?

• G01N 21/3586 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared lightInvestigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using Terahertz radiation by Terahertz time domain spectroscopy [THz-TDS]

Abstract

Provided are variants of alpha-1-antitrypsin comprising mutations which render the variants oxidation-as well as protease-resistant, polynucleotides encoding said variants, methods of producing the variants and the variants for use in the treatment of alpha-1-antitrypsin deficiency, cystic fibrosis and chronic obstructive pulmonary disease.

IPC Classes  ?

• C07K 14/81 - Protease inhibitors
• A61K 38/00 - Medicinal preparations containing peptides

Abstract

Allergy is one of the world's most common chronic conditions. The state of the art method for allergy testing is the skin prick test (SPT) where different allergens are introduced into the skin of the tested person. This method is at best semi-quantitative, a relatively low number of tests can be performed and patients might experience unpleasant reactions. This invention is a novel allergy test providing fast, accurate and quantitative monitoring of allergic reactions in the skin. As a novelty, microneedle-based electrochemical sensor arrays are used to introduce allergens in the skin and perform electrochemical detection of biomarkers released from activated mast cells in the interstitial fluid.

IPC Classes  ?

• A61B 5/00 - Measuring for diagnostic purposes Identification of persons
• A61B 5/145 - Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value
• A61B 5/1473 - Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value using chemical or electrochemical methods, e.g. by polarographic means invasive, e.g. introduced into the body by a catheter

Abstract

Disclosed is a method for optimizing optical fibre based transmission, in particular increasing span length and/or minimizing energy consumption and cost for an optical fibre based transmission link, and the so optimized fibre based transmission link. Further disclosed is a method for minimizing the energy consumption and/or the cost of an optical fibre based transmission link by doubling the number of fibre channels. Also disclosed is a method for minimizing the energy consumption and/or the cost of a repeaterless optical fibre based transmission link.

IPC Classes  ?

• H04B 10/293 - Signal power control
• H04B 10/079 - Arrangements for monitoring or testing transmission systemsArrangements for fault measurement of transmission systems using an in-service signal using measurements of the data signal
• H04B 10/25 - Arrangements specific to fibre transmission

Abstract

Disclosed are a clinical support system and associated method for automatic real-time detection of clinical deterioration events in a patient. Existing clinical support systems typically rely on alarm generation based on simple threshold values. This often results in too many alarms, including false alarms, which consequently results in alarm fatigue in the medical staff or in the ignoring of the alarms. The presently disclosed system and method provides an alternative to existing clinical support systems, since it incorporates clinically validated computer-implemented subroutines that provides a higher predictive value to the medical staff. The subroutines utilize thresholds and time durations, which have been clinically evaluated such that false alarms are reduced while keeping the most relevant alarms, i.e. alarms that require clinical action. Further disclosed is a computer program configured to execute the disclosed method, thereby providing automatic real-time detection of clinical deterioration events in a patient.

IPC Classes  ?

• A61B 5/00 - Measuring for diagnostic purposes Identification of persons
• A61B 5/0205 - Simultaneously evaluating both cardiovascular conditions and different types of body conditions, e.g. heart and respiratory condition
• A61B 5/021 - Measuring pressure in heart or blood vessels
• A61B 5/08 - Measuring devices for evaluating the respiratory organs
• A61B 5/145 - Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value
• A61B 5/363 - Detecting tachycardia or bradycardia

Abstract

Provided are methods for increasing the growth of Moorella species bacteria, genetically modified bacteria derived from such methods, and use of such bacteria for metabolizing a carbon-containing substrate, optionally in the production of a biochemical.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• C12P 7/00 - Preparation of oxygen-containing organic compounds
• C12R 1/145 - Clostridium

Abstract

The invention presented herein relates to yeast cells and methods for production of betalains.

IPC Classes  ?

• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)

Abstract

The invention provides specific Tn- and/or STn antibody libraries and methods for identifying specific antibodies, which target Tn- and/or STn-glycosylation sites of any glycoprotein of choice, especially glycoproteins of cancer cell targets. The invention further provides antibodies identified by the new concept proposed herein, which have combined specificity towards both the sugar epitope as well as the peptide epitope of the glycoprotein.

IPC Classes  ?

• C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
• A61K 49/00 - Preparations for testing in vivo
• C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

The present invention relates to a liquid phase transmission electron microscopy (LP-TEM) holder with integrated temperature regulation for operating with an associated transmission electron microscopy instrument providing an electron beam for imaging, the LP-TEM holder comprising a liquid phase sample receptacle (LPSR, 5) which may be made from two or more layers of materials. e.g. microchips, the LPSR providing a liquid compartment. The LP-TEM holder furthermore comprises an integrated temperature regulating unit (15), capable of regulating the temperature of the LPSR and liquid in the liquid compartment by means of a temperature measuring unit (16) capable of measuring a temperature in the liquid compartment. The LPSR is thermally isolated with a thermal isolating portion (30), with respect to an external environment and associated devices. The invention is particularly advantageous for providing fast temperature regulation and accurate steady state temperatures of one or more fluids to be imaged.

IPC Classes  ?

• H01J 37/20 - Means for supporting or positioning the object or the materialMeans for adjusting diaphragms or lenses associated with the support
• H01J 37/26 - Electron or ion microscopesElectron- or ion-diffraction tubes

Abstract

A phototherapeutic apparatus comprising a plurality of light sources and a control circuit, the plurality of light sources being arranged in a spatial pattern so as to form a display having a display area and each of the plurality of light sources being individually controllable to emit colored light; wherein the control circuit is configured to: receive image data; control the plurality of light sources to emit a spatially resolved pattern of emitted colored light, the spatially resolved pattern representing the received image data, wherein each of the plurality of light sources emits colored light perceivable by a human observer as having respective user-perceptible target image colors; wherein the control circuit is configured to control at least each of a first subset of the plurality of light sources to alternatingly emit respective first and second light, alternating at a color-flicker frequency; the first light having a first set of color components resulting in a first emitted color and the second light having a second set of color components resulting in a second emitted color; wherein the color-flicker frequency is selected high enough to cause the alternatingly emitted first and second light to be perceived by a human observer as light having a user-perceptible fused color corresponding to the corresponding target image color.

IPC Classes  ?

• A61N 5/06 - Radiation therapy using light
• A61M 21/00 - Other devices or methods to cause a change in the state of consciousnessDevices for producing or ending sleep by mechanical, optical, or acoustical means, e.g. for hypnosis

Abstract

The disclosure relates to a process for the manufacture of a steel component comprising a fine-grained martensite structure component. The process comprises the steps of providing a steel component having an initial steel composition; introducing nitrogen into the steel component at a temperature T1 above 950° C., thereby creating an at least partly austenitic nitrogen-containing steel component; bringing the at least partly austenitic nitrogen-containing steel component to a temperature T2, such that austenite is decomposed into a steel component comprising at least an amount of carbon- and/or nitrogen-containing precipitates; bringing the steel component comprising at least an amount of carbon- and/or nitrogen-containing precipitates to a temperature T3 which is above T2, thereby creating an at least partly austenitic nitrogen-containing steel component optionally comprising at least an amount of carbon- and/or nitrogen-containing precipitates; and bringing the at least partly austenitic nitrogen-containing steel component to a temperature T4 that is below a martensite start temperature of the at least partly austenitic nitrogen-containing steel component for initiating transformation of at least some of the austenite into fine-grained martensite, thereby producing a steel component comprising a fine-grained martensite structure component.

IPC Classes  ?

• C21D 9/00 - Heat treatment, e.g. annealing, hardening, quenching or tempering, adapted for particular articlesFurnaces therefor
• C21D 1/18 - HardeningQuenching with or without subsequent tempering
• C21D 1/74 - Methods of treatment in inert gas, controlled atmosphere, vacuum or pulverulent material
• C21D 1/773 - Methods of treatment in inert gas, controlled atmosphere, vacuum or pulverulent material under reduced pressure or vacuum
• C21D 6/00 - Heat treatment of ferrous alloys
• C21D 6/04 - Hardening by cooling below 0° C
• C22C 38/00 - Ferrous alloys, e.g. steel alloys
• C22C 38/18 - Ferrous alloys, e.g. steel alloys containing chromium
• C23C 8/22 - Carburising of ferrous surfaces
• C23C 8/26 - Nitriding of ferrous surfaces
• C23C 8/32 - Carbo-nitriding of ferrous surfaces
• C23C 8/38 - Treatment of ferrous surfaces
• C23C 8/80 - After-treatment

Abstract

An apparatus includes a processing pipeline with a stationary structure motion corrector, a stationary structure remover and a flowing structure detector. The stationary structure motion corrector is configured to motion correct stationary structure in a series of ultrasound images for subject motion, wherein the series of ultrasound images includes the stationary structure and flowing structure. The stationary structure remover is configured to remove the stationary structure from the motion corrected series of ultrasound images thereby producing flow images of the flowing structure. The flowing structure detector is configured to detect peaks of flow of the flowing structure in the flow images over time to generate images of detected flow peaks. The images of detected flow peaks are accumulated over time to generate a super resolution ultrasound image.

IPC Classes  ?

• A61B 8/08 - Clinical applications
• A61B 8/00 - Diagnosis using ultrasonic, sonic or infrasonic waves
• A61B 8/06 - Measuring blood flow

Abstract

The present disclosure relates to an integrated optical phased array (OPA) with a large field of view (FOV), possibly up to 180 degrees FOV. One embodiment regards a chip integrated optical phased array, comprising: a phase shifter array having N input light channels and configured for tuning the phase of the N input light channels; a beam splitter configured for splitting light from a light source into the N input channels of the phase shifter array; a waveguide array configured for squeezing the phase tuned N light channels to a narrow pitch output end such that the light form N channels interfere to form a plane wave at the output end; and a plane emitting surface configured for transforming the plane wave to light emittance out of the chip with a predefined field of view (FOV). Another embodiment regards an OPA with an on-chip beam expander for small beam divergence with a relatively low number of channels.

IPC Classes  ?

• G02F 1/295 - Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulatingNon-linear optics for the control of the position or the direction of light beams, i.e. deflection in an optical waveguide structure
• G02F 1/29 - Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulatingNon-linear optics for the control of the position or the direction of light beams, i.e. deflection

Abstract

The present invention relates to a cell capable of producing a fluorinated, a chlorinated or a brominated compound, methods for producing fluorinated, chlorinated or brominated compounds in a cell and expression systems therefor.

IPC Classes  ?

• C12P 19/40 - Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/10 - Transferases (2.)
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
• C12R 1/40 - Pseudomonas putida

Abstract

The present invention relates to a method for producing a person-tailored T cell composition by in vitro stimulation and expansion of T cells comprising the steps of i) providing at least one identified HLA haplotype from a subject; ii) preparing at least one APC comprising at least one HLA haplotype corresponding to said at least one identified HLA haplotype; and at least one antigenic peptide matched to said at least one HLA haplotype; wherein said at least one antigenic peptide comprises an epitope from Merkel cell polyomavirus, said epitope originates from large T antigen (LTA), small T antigen (STA) or the shared region (CT) of LTA and STA; iii) providing a sample comprising T cells, iv) contacting said sample with an expansion solution comprising at least one APC as prepared in step ii, v) stimulating and expanding T cells with specificity for said at least one antigenic peptide comprised on at least one APC in culture, and optionally harvesting the T cells from the culture, to obtain a person-tailored T cell composition.

IPC Classes  ?

• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• C07K 14/74 - Major histocompatibility complex [MHC]
• C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells

Abstract

Disclosed is a method of 3D-printing a polymer-based structure comprising at least one polymer-based substructure. The method comprised the steps of depositing a photocurable resin onto a spinnable substrate, spin coating a layer comprising said photocurable resin on said spinnable substrate by spinning said spinnable substrate, and irradiating said layer, while spinning said spinnable substrate, at one or more selected positions with a light source to cure at least a portion of said layer, thereby forming a polymer-based substructure. A system for 3D-printing a polymer-based structure comprising at least one polymer-based substructure is further disclosed.

IPC Classes  ?

• B29C 64/241 - Driving means for rotary motion
• B29C 64/129 - Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask
• B29C 64/245 - Platforms or substrates
• B29C 64/268 - Arrangements for irradiation using laser beamsArrangements for irradiation using electron beams [EB]
• B33Y 10/00 - Processes of additive manufacturing
• B33Y 30/00 - Apparatus for additive manufacturingDetails thereof or accessories therefor

Abstract

The present disclosure provides peptide conjugated drugs based on MuV SH Protein, their use as therapeutic agents and their use to provide delivery to and/or transfer across a membrane of the drug. The present disclosure provides use of the peptide conjugated drug for delivery of the drug to and/or across a membrane harbouring G protein-coupled receptor 125 (GPR125), such as the choroid plexus.

IPC Classes  ?

• A61K 47/64 - Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
• A61K 38/16 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
• A61K 38/26 - Glucagons

Abstract

The present invention relates to immune stimulating nanoparticle compositions, and their use in treatment of diseases and disorders, such as cancer. In particular, the present invention relates to nanoparticle compositions comprising a TLR7 agonist, such as 1V270.5

IPC Classes  ?

• A61K 31/675 - Phosphorus compounds having nitrogen as a ring hetero atom, e.g. pyridoxal phosphate
• A61K 9/107 - Emulsions
• A61K 9/127 - Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
• A61K 9/51 - Nanocapsules
• A61K 31/4745 - QuinolinesIsoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having nitrogen as a ring hetero atom, e.g. phenanthrolines
• A61K 45/06 - Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• A61P 35/00 - Antineoplastic agents

Abstract

The present disclosure relates to a method of manufacturing a transferable lamella comprising interconnected nanostructures, the method comprising the steps of: a) providing a substrate such as a planar substrate; b) forming at least one superstructure on the substrate, said superstructure comprising a plurality of elongated nanostructures (formed e.g. by growth, deposition, and/or etching); wherein the elongated nanostructures are formed such that at least two of said nanostructures are conductively interconnected, and/or wherein at least a first layer is grown or deposited to conductively interconnect or insulate at least a part of the elongated nanostructures; c) encapsulating at least a portion of said superstructure in an encapsulating material, said portion comprising at least two interconnected nanostructures; and d) cutting the encapsulating material in a direction that intersects at least two interconnected nanostructures, thereby manufacturing a transferable lamella comprising interconnected nanostructures. The present disclosure further relates to an electronic device manufactured from one or more of the lamellas provided by the method.

IPC Classes  ?

• C30B 29/60 - Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape characterised by shape
• C30B 11/12 - Vaporous components, e.g. vapour-liquid-solid-growth
• C30B 29/40 - AIIIBV compounds
• C30B 29/66 - Crystals of complex geometrical shape, e.g. tubes, cylinders
• H01L 29/06 - Semiconductor bodies characterised by the shapes, relative sizes, or dispositions of the semiconductor regions
• H01L 29/20 - Semiconductor bodies characterised by the materials of which they are formed including, apart from doping materials or other impurities, only AIIIBV compounds
• H01L 29/66 - Types of semiconductor device
• H01L 29/76 - Unipolar devices
• H01L 31/0352 - SEMICONDUCTOR DEVICES NOT COVERED BY CLASS - Details thereof characterised by their semiconductor bodies characterised by their shape or by the shapes, relative sizes or disposition of the semiconductor regions
• H01L 33/06 - SEMICONDUCTOR DEVICES NOT COVERED BY CLASS - Details thereof characterised by the semiconductor bodies with a quantum effect structure or superlattice, e.g. tunnel junction within the light emitting region, e.g. quantum confinement structure or tunnel barrier
• H10N 60/12 - Josephson-effect devices

Abstract

The invention relates to a mechanical oscillator device comprising an unsupported membrane with a multitude of discrete mass elements distributed to form Phononic crystal cells in the form of regions of additional mass each comprising a plurality of mass elements. The phononic crystal structure has a defect for confining a mechanical oscillation mode having a resonance frequency, f, with the mass elements have a smallest lateral dimension of less than 1/10 of a wavelength of the mechanical oscillation mode. The invention is based on a distribution of tiny additional mass elements providing a periodic density contrast pattern to create the bandgap. This approach keeps the tensile stress uniform which ensures perfect overlap between the tensile stress distribution and mode-shape. This again reduces the damping and thus allows for very high quality factors, Q.

IPC Classes  ?

• H03H 9/02 - Networks comprising electromechanical or electro-acoustic elementsElectromechanical resonators Details
• G01H 13/00 - Measuring resonant frequency

Abstract

The invention regards a method for electrochemical ammonia synthesis, comprising the steps of: —providing an electrolysis cell having a cathode, —contracting the cathode with a source of cations preferably lithium cations, a source of nitrogen a source of oxygen, and a source of protons, wherein the oxygen source provides a predefined oxygen concentration, and —subjecting the cell to a potential and current load, whereby ammonia is synthesized.

IPC Classes  ?

• C25B 15/08 - Supplying or removing reactants or electrolytesRegeneration of electrolytes
• C25B 1/27 - Ammonia
• C25B 9/05 - Pressure cells
• C25B 9/65 - Means for supplying currentElectrode connectionsElectric inter-cell connections
• C25B 15/02 - Process control or regulation

Abstract

The present invention relates to an AC-DC power converter which includes a resonant DC-DC converter and a charge pump circuit. The charge pump circuit is configured to perform power factor correction of the AC-DC power converter by drawing current pulses at a switching frequency of the converter from an AC line voltage such that electrical charges of the current pulses vary substantially proportionally with instantaneous amplitude of the AC line voltage.

IPC Classes  ?

• H02M 1/42 - Circuits or arrangements for compensating for or adjusting power factor in converters or inverters
• H02M 1/00 - Details of apparatus for conversion
• H02M 3/07 - Conversion of DC power input into DC power output without intermediate conversion into AC by static converters using resistors or capacitors, e.g. potential divider using capacitors charged and discharged alternately by semiconductor devices with control electrode
• H02M 3/335 - Conversion of DC power input into DC power output with intermediate conversion into AC by static converters using discharge tubes with control electrode or semiconductor devices with control electrode to produce the intermediate AC using devices of a triode or a transistor type requiring continuous application of a control signal using semiconductor devices only

Abstract

A spray forming method for producing a metallic ingot and metallic powder from a metallic source of metal or metal alloy includes: forming one or more streams of metal or alloy from the source, gas atomizing one or more streams of metal or alloy to form one or more sprays of atomized droplets, directing the spray(s) of droplets through a spray nozzle to a rotatable hot body, depositing the droplets to the hot body to form the ingot, controlling the process parameters 1) temperature of metal or alloy, 2) inlet and outlet pressure of the spray nozzle, 3) rotation speed of the hot body, and/or 4) distance between the hot body and the spray(s) of droplets, and collecting the metallic powder having a predefined size distribution. The process parameters are controlled such that the ingot yield is 60-80% and the metallic powder yield is 40-20%, relative to the metallic source.

IPC Classes  ?

• B22F 9/08 - Making metallic powder or suspensions thereofApparatus or devices specially adapted therefor using physical processes starting from liquid material by casting, e.g. through sieves or in water, by atomising or spraying
• B22F 1/05 - Metallic powder characterised by the size or surface area of the particles
• B33Y 80/00 - Products made by additive manufacturing

Abstract

Disclosed herein are methods of producing glyco-modified viral antigens that provide a shift of the glycosylation profile of recombinant produced viral antigens (e.g. glycoproteins) towards the naturally occurring viral antigens (e.g. glycoproteins). Disclosed are methods of producing a modified viral antigen comprising expressing a viral antigen in a recombinant mammalian cell line having one or more of the endogenous genes Mgat2, Mgat4A, Mgat4B, Mgat5, St3Gal3, St3Gal4, B4galt1, B4galt2, B4galt3, B4galt4, B4galt5, B3gnt2, St3Gal6, SPPL3, and/or FUT8 inactivated and/or downregulated; and optionally a gene ST6Gall inserted. Disclosed are glyco-modified viral antigens produced by the method of using a recombinant mammalian cell line. Disclosed are methods of treating a subject in need thereof comprising administering a composition comprising a therapeutically effective amount of one or more of the glyco-modified viral antigens. Disclosed are methods of screening for an antibody specific to one or more of the disclosed glyco-modified viral antigens.

IPC Classes  ?

• A61K 39/29 - Hepatitis virus
• A61K 49/00 - Preparations for testing in vivo
• C07K 1/107 - General processes for the preparation of peptides by chemical modification of precursor peptides
• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
• C12N 15/86 - Viral vectors
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The invention relates to a microbiome-based therapeutic composition comprising an engineered probiotic cell expressing a sulfotransferase and the use of the microbiome-based therapeutic composition in medicine and a therapeutic regimen.

IPC Classes  ?

• A61K 38/45 - Transferases (2)
• A61K 35/741 - Probiotics
• A61K 36/064 - Saccharomycetales, e.g. baker's yeast
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Abstract

The present disclosure relates to methods for production of (Z)-11-hexadecen-1-ol in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecenal in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecen-1-yl acetate in a yeast cell. The disclosure also provides for nucleic acid constructs and yeast cells useful for performing the present methods, as well as to pheromone compositions.

IPC Classes  ?

• C07K 14/435 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
• C12N 1/16 - YeastsCulture media therefor
• C12P 7/04 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
• C12P 7/24 - Preparation of oxygen-containing organic compounds containing a carbonyl group
• C12P 7/6436 - Fatty acid esters

Abstract

A measurement based photonic quantum computing system includes an optical input generator configured for receiving first optical paired inputs, and second optical paired inputs, inducing a first predefined relative time delay into each optical paired inputs for making one mode of the first and one mode of the second optical paired inputs into coexisting optical inputs, representing a logic level of the quantum computing system, a delocalized measurement architecture configured for optically interfering coexisting optical inputs for generating intermediate outputs, optically interfering by first and second variable path-couplers for each intermediate output a first mode of the intermediate outputs to a second mode of first or second neighboring intermediate outputs, respectively, for generating interfered outputs, and detecting by two optical detectors, preferably homodyne optical detectors, each mode of the intermediate outputs and interfered outputs, such that the delocalized measurement architecture is configured for gate implementation of the quantum computing system.

IPC Classes  ?

• G06N 10/20 - Models of quantum computing, e.g. quantum circuits or universal quantum computers

Abstract

A method and apparatus for X-ray diffraction analysis of amorphous and/or semi-crystalline materials is able to provide the internal strain map of at least the exposed region of the material, even in the amorphous regions of the material. This is achieved in part by providing several unique and novel analysis methods that are able to extract material properties of semi-crystalline and amorphous materials based on the amorphous diffraction signal. The ability to analyse the amorphous diffraction signal is further facilitated by the use of one or more state-of-the-art energy dispersive detectors, which the inventors have found especially suitable for this purpose. This further allows the use of a polychromatic X-ray source as opposed to the monochromatic X-ray sources typically encountered in X-ray diffraction experiments.

IPC Classes  ?

• G01N 23/207 - Diffractometry, e.g. using a probe in a central position and one or more displaceable detectors in circumferential positions

Abstract

A waveguide-coupled cavity structure configured to exhibit Fano resonance (such as asymmetric Fano resonance or symmetric Fano resonance (inverse Lorentzian resonance), is utilized in an optical receiver or a method for direct detection of coherent optical signals by converting a phase-modulation on a coherent optical signal into an intensity-modulation of the optical signal. The waveguide-coupled cavity structure is designed for a transmission spectrum of the Fano resonance to overlap with a spectrum of the In modulated coherent optical signal to suppress transmission of at least one sideband of the modulated coherent optical signal through the structure, the sideband suppression being asymmetrical with respect to the carrier frequency of the modulated coherent optical signal. The invention may be used for direct detection of more advanced coherent modulation formats such as quadrature phase-shift keying (QPSK) signals and high order quadrature amplitude modulation (n-QAM) signals.

IPC Classes  ?

• H04B 10/61 - Coherent receivers
• H04B 10/40 - Transceivers
• H04B 10/50 - Transmitters

Abstract

The present invention concerns oxidative degradation of polysaccharides, and provides a composition, kit, and method for increasing the efficiency of recalcitrant cellulose degradation, using an auxiliary activity family 7 (AA7) enzymes and a polysaccharide monooxygenase enzyme (E.C. 1.14.99.-). Said AA7 enzyme comprises dehydrogenase activity (E.C. 1.1.1.-) and gluco-oligosaccharide oxidase activity (E.C. 1.1.3.-) and provides a novel “functionality” within a previously not described Glade of AA7 dehydrogenases.

IPC Classes  ?

• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase

Abstract

A method of manufacturing a water-soluble transfer stack comprising multilayer two-dimensional material includes: i. Providing a growth stack comprising a growth substrate and a two-dimensional material layer; ii. Applying an intercalating solution, to the growth stack; iii. Applying a transfer layer comprising a water-soluble polymer film, to the growth stack; iv. Delaminating the water-soluble polymer film together with the two-dimensional material layer, thereby obtaining a delaminated film, from the growth substrate; and v. Repeating steps i.-iv. a number of times, wherein the delaminated film is used as the transfer layer; thereby manufacturing a water-soluble transfer stack comprising multilayer two-dimensional material.

IPC Classes  ?

• H01L 21/02 - Manufacture or treatment of semiconductor devices or of parts thereof
• H01L 21/683 - Apparatus specially adapted for handling semiconductor or electric solid state devices during manufacture or treatment thereofApparatus specially adapted for handling wafers during manufacture or treatment of semiconductor or electric solid state devices or components for supporting or gripping

Abstract

A switched-capacitor balancing and characterization system for balancing and characterization of a plurality of stacked battery cells includes at least one capacitor connectable to a plurality of battery cells via switches, configured to connect or disconnect the at least one capacitor to the battery cells and a processing unit configured to control the plurality of switches. The switched-capacitor balancing and characterization system is configured to perform simultaneous balancing and characterization of the battery cells. The disclosure further relates to a battery system comprising the switched-capacitor balancing and characterization system and the stack of battery cells and to a method for simultaneous balancing and characterization of a stack of battery cells, using a switched-capacitor balancing and characterization system and/or using a battery system.

IPC Classes  ?

• H02J 7/00 - Circuit arrangements for charging or depolarising batteries or for supplying loads from batteries
• G01R 31/389 - Measuring internal impedance, internal conductance or related variables

Abstract

The invention relates to a method for preparing a substrate comprising a sample reception area and a sensing area. The method comprises the steps of: 1) applying a sample on the sample reception area; 2) rotating the substrate around a predetermined axis; 3) during rotation, at least part of the liquid travels from the sample reception area to the sensing area due to capillary forces acting between the liquid and the substrate; and 4) removing the wave of particles and liquid formed at one end of the substrate. The sensing area is closer to the predetermined axis than the sample reception area. The sample comprises a liquid part and particles suspended therein.

IPC Classes  ?

• G01N 1/40 - Concentrating samples
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 21/07 - Centrifugal type cuvettes
• G01N 21/65 - Raman scattering
• G01N 33/49 - Physical analysis of biological material of liquid biological material blood
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor

Abstract

The present invention relates to thermophilic cells and methods for the microbial production of volatile compounds, including acetone, butanone and isopropanol. Also provided are nucleic acid constructs, vectors and host cells useful in such methods.

IPC Classes  ?

• C12P 7/28 - Acetone-containing products
• C12P 7/04 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
• C12N 9/10 - Transferases (2.)
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12N 9/88 - Lyases (4.)
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Abstract

The present disclosure relates to a remote characterization of optical components in multi-span fiber links, the characterization based on a parametric model of optical components, such as a machine learning model. The present disclosure also relates to the use of the presently disclosed model for optimization of a link, based on an arbitrary optimization strategy related to the spectral power profile and the quality of service of the link.

IPC Classes  ?

• H04J 14/02 - Wavelength-division multiplex systems

Abstract

A method for producing a multi-material three-dimensional object includes: computing projections describing the object from different orientation angles, including: a number of first projections describing a first part of said object to be formed in a first material; a number of second projections describing a second part of said object to be formed in a second material; providing a build volume comprising: a first photosensitive component for polymerizing into the first material upon irradiation by a first wavelength light; a second photosensitive component for polymerizing into the second material upon irradiation by a second wavelength light, the second material having different mechanical properties than the first; and irradiating the build volume with a number of patterns of light, at the respective corresponding orientations/wavelengths such that the first wavelength light deposits energy according to a first energy distribution and the second wavelength light deposits energy according to a second energy distribution.

IPC Classes  ?

• B29C 41/22 - Making multilayered or multicoloured articles
• B29C 64/124 - Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
• B29C 64/129 - Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask
• B29C 64/277 - Arrangements for irradiation using multiple radiation means, e.g. micromirrors or multiple light-emitting diodes [LED]
• B29C 64/393 - Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
• B33Y 10/00 - Processes of additive manufacturing
• B33Y 30/00 - Apparatus for additive manufacturingDetails thereof or accessories therefor
• B33Y 50/02 - Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
• C08F 2/46 - Polymerisation initiated by wave energy or particle radiation
• C08F 20/06 - Acrylic acidMethacrylic acidMetal salts or ammonium salts thereof
• C08L 63/00 - Compositions of epoxy resinsCompositions of derivatives of epoxy resins
• B29K 33/00 - Use of polymers of unsaturated acids or derivatives thereof, as moulding material
• B29K 33/04 - Polymers of esters
• B29K 63/00 - Use of epoxy resins as moulding material

Abstract

The present invention relates to a drug delivery platform. In particular, it relates to a foil-based drug delivery platform for delivery to the intestine.

IPC Classes  ?

• A61K 9/00 - Medicinal preparations characterised by special physical form
• A61K 38/28 - Insulins
• A61K 35/741 - Probiotics

Abstract

The present invention relates to an engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system comprising engineered dual guide nucleic acids (e.g., RNAs) capable of activating a CRISPR-Associated (Cas) nuclease, such as a type V-A Cas nuclease. Also provided are methods of targeting, editing, and/or modifying a nucleic acid using the engineered CRISPR system, and compositions and cells comprising the engineered CRISPR system.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12N 15/90 - Stable introduction of foreign DNA into chromosome

Abstract

The present disclosure relates to an inspection system, in particular a mobile inspection system, for outdoor inspection of photovoltaic modules in situ, the inspection system comprising a movable detector unit and a processing unit. The detector unit is configured to obtain, when positioned at a user-controllable position spaced apart from a photovoltaic module of a photovoltaic power system, luminescence data from a light-receiving surface of said photovoltaic module. The detector unit may include a light source, in particular a laser source, configured to emit an illumination beam, means for selectively directing the illumination beam onto respective portions of the light-receiving surface so as, when the illumination beam is directed onto a first portion of the light-receiving surface, to only illuminate the first portion while leaving a corresponding first remaining portion of the light-receiving surface unilluminated by the illumination beam.

IPC Classes  ?

• H02S 50/15 - Testing of PV devices, e.g. of PV modules or single PV cells using optical means, e.g. using electroluminescence

Abstract

Embodiments disclosed herein include novel nucleic acid-guided nucleases, novel guide nucleic acids, and novel targetable nuclease systems, and methods of use. In some embodiments, engineered non-naturally occurring nucleic acid-guided nucleases, can be used with known guide nucleic acids in a targetable nuclease system. In certain embodiments, targetable nuclease systems can be used to edit targeted genomes of humans and other species. In some embodiments, methods include, but are not limited to, recursive genetic engineering and trackable genetic engineering methods.

IPC Classes  ?

• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 9/22 - Ribonucleases

Abstract

Embodiments disclosed herein include novel nucleic acid-guided nucleases, novel guide nucleic acids, and novel targetable nuclease systems, and methods of use. In some embodiments, engineered non-naturally occurring nucleic acid-guided nucleases, can be used with known guide nucleic acids in a targetable nuclease system. In certain embodiments, targetable nuclease systems can be used to edit targeted genomes of humans and other species. In some embodiments, methods include, but are not limited to, recursive genetic engineering and trackable genetic engineering methods.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/90 - Stable introduction of foreign DNA into chromosome

Abstract

The invention provides a microbial production cell for synthesis of a product, further comprising a burden-addiction genetic circuit whose expression confers a selective growth and/or survival advantage on those cells that synthesize the product; while limiting proliferation of low- or non-productive escaper cells.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli

Abstract

Herein are disclosed bacterial cells useful for production of 2-fluoro-cis,cis-muconate and derivatives thereof. The disclosure also provides methods and nucleic acid constructs therefor.

IPC Classes  ?

• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas

Abstract

The present invention relates to c-di-GMP lowering chemical compounds having anti- biofilm properties. In particular, the present invention relates to anti-biofilm compounds or salts or tautomers thereof for use in treatment and/or prevention of bacterial biofilm infection in human subjects caused by biofilm-forming bacteria of the genus Pseudomonas, in particular Pseudomonas spp. including P. aeruginosa. Methods of treating such infections in human subjects are contemplated as well. The present inventions further relates to the use of an anti-biofilm compound or a salt or tautomer thereof for dispersing biofilms in industrial water systems.

IPC Classes  ?

• A61K 31/415 - 1,2-Diazoles
• A61K 31/635 - Compounds containing para-N-benzene- sulfonyl-N-groups, e.g. sulfanilamide, p-nitrobenzenesulfonohydrazide having a heterocyclic ring, e.g. sulfadiazine
• A61K 31/4439 - Non-condensed pyridinesHydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
• A61K 31/4709 - Non-condensed quinolines containing further heterocyclic rings
• A61K 31/42 - Oxazoles
• A61P 31/04 - Antibacterial agents
• A61K 31/702 - Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
• A61K 31/496 - Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
• A61P 13/02 - Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
• A61K 9/00 - Medicinal preparations characterised by special physical form
• A01N 43/56 - 1,2-DiazolesHydrogenated 1,2-diazoles
• A01N 43/80 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms, as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
• A01P 1/00 - DisinfectantsAntimicrobial compounds or mixtures thereof
• C02F 1/50 - Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment

Abstract

An apparatus for the separation of dry matter and liquid from a medium includes: a plurality of press rollers and at least one pore roller, at least one separation chamber for receiving the medium, and at least one filtrate chamber defined in cross section by press rollers and the at least one pore roller. The apparatus is configured for establishing a relative negative pressure inside the pore roller interior, such that liquid in the medium is sucked into the pore roller interior, and for roller rotation such that during separation operation dry matter of the medium initially passes between the pore roller and a press roller when transferring from the separation chamber to the filtrate chamber, and subsequently passes between two press rollers, when exiting from the filtrate chamber.

IPC Classes  ?

• B01D 33/06 - Filters with filtering elements which move during the filtering operation with rotary cylindrical filtering surfaces, e.g. hollow drums
• B01D 33/80 - Accessories
• B01D 33/46 - Regenerating the filter material in the filter by scrapers, brushes or the like acting on the cake-side of the filtering element

Abstract

The invention provides an invasive recombinant bacterial cell for use in prevention and/or treatment of an immune-related disorder; said bacterial cell comprising one or more recombinant nucleic acid molecule(s) encoding one or more therapeutic agent(s) for use in prevention and/or treatment of said immune-related disease in a mammal in need thereof.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
• A61K 38/16 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
• A61K 38/46 - Hydrolases (3)
• A61P 37/02 - Immunomodulators

Abstract

The present invention relates to artificial antigen presenting cell (aAPC) scaffolds to provide cells with specific functional stimulation to obtain phenotypic and functional properties ideal to mediate tumor regression or viral clearance. In particular, the scaffolds of the present invention comprise antigens, such as peptide-MHC (pMHC) class I molecules, and specific combinations of cytokines and co-stimulatory molecules to allow effective expansion and functional stimulation of specific T cells.

IPC Classes  ?

• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• A61K 35/17 - LymphocytesB-cellsT-cellsNatural killer cellsInterferon-activated or cytokine-activated lymphocytes
• A61K 39/12 - Viral antigens
• A61K 39/145 - Orthomyxoviridae, e.g. influenza virus
• A61K 39/245 - Herpetoviridae, e.g. herpes simplex virus
• A61P 31/16 - Antivirals for RNA viruses for influenza or rhinoviruses
• A61P 31/22 - Antivirals for DNA viruses for herpes viruses
• A61P 35/00 - Antineoplastic agents
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
• C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof

Abstract

A grating reflector. The grating reflector includes a mesh structure defining a mesh plane and having a thickness normal thereto. The mesh structure includes parallel bars and parallel crossbars, which extend along a direction orthogonal to the bars. The bars and crossbars define a 2D grid of elongated holes, each extending through the mesh structure perpendicular to the mesh plane. The holes are elongated along a direction parallel to the bars and have a substantially rectangular shape with rounded corners. The 2D grid is defined by a cross-shaped unit cell having a bar section and an intersecting crossbar section. The grating reflector has a reflectivity in a bandwidth around a center wavelength higher than 0.99. A ratio between the unit cell volume and the center wavelength in the mesh material cubed is between 1.35 and 1.55.

IPC Classes  ?

• B81B 3/00 - Devices comprising flexible or deformable elements, e.g. comprising elastic tongues or membranes
• H01S 5/183 - Surface-emitting [SE] lasers, e.g. having both horizontal and vertical cavities having only vertical cavities, e.g. vertical cavity surface-emitting lasers [VCSEL]

Abstract

A power converter is embodied on a semiconductor substrate member and has a first region with a passive electrical component with a first electrically conductive layer pattern of an electrically conductive material and a second electrically conductive layer pattern of an electrically conductive material deposited on respective sides of the semiconductor substrate member. A trench or through-hole is formed (by etching) in the substrate within the first region, and the electrically conductive material is deposited at least on a bottom portion of the trench or on a sidewall of the through-hole and electrically connected to one or both of the first conductive layer pattern and the second conductive layer pattern. A second region has an active semiconductor component integrated with the semiconductor substrate by being fabricated by a semiconductor fabrication process. There is also provided a power supply, such as a DC-DC converter, embedded the semiconductor substrate member.

IPC Classes  ?

• H02M 3/15 - Conversion of DC power input into DC power output without intermediate conversion into AC by static converters using discharge tubes with control electrode or semiconductor devices with control electrode using devices of a triode or transistor type requiring continuous application of a control signal using discharge tubes only
• H01L 49/02 - Thin-film or thick-film devices
• H01F 17/06 - Fixed inductances of the signal type with magnetic core with core substantially closed in itself, e.g. toroid
• H01L 23/48 - Arrangements for conducting electric current to or from the solid state body in operation, e.g. leads or terminal arrangements
• H01L 23/522 - Arrangements for conducting electric current within the device in operation from one component to another including external interconnections consisting of a multilayer structure of conductive and insulating layers inseparably formed on the semiconductor body
• H02M 3/156 - Conversion of DC power input into DC power output without intermediate conversion into AC by static converters using discharge tubes with control electrode or semiconductor devices with control electrode using devices of a triode or transistor type requiring continuous application of a control signal using semiconductor devices only with automatic control of output voltage or current, e.g. switching regulators

Abstract

The invention relates to microbial cells and microbial cells for use as a medicament, the cells expressing a recombinant nucleic acid encoding a eukaryotic tyrosine hydroxylase. The cells produce L-DOPA and dopamine.

IPC Classes  ?

• A61K 38/44 - Oxidoreductases (1)
• C12N 15/52 - Genes encoding for enzymes or proenzymes

Abstract

A laboratory-based 3D scanning X-ray scanning Laue micro-diffraction system and method for characterisation of crystalline materials, comprising: a focusing optic, a sample located at a distance from the focusing optic, a laboratory X-ray source, a stage to translate and rotate the sample, a detector arranged to detect the Laue diffraction patterns of the diffracted X-rays. The method comprising scanning each layer of the sample by translating the sample relatively to the focused beam at different rotations to illuminate each voxel in the layer in more than one rotation and indexing each voxel within the layer using the recorded Laue diffraction patterns at different rotations. By repeating the translation and rotation for different layers of the sample, a 3D image of the grain structure of the sample is reconstructed.

IPC Classes  ?

• G01N 23/00 - Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups , or
• G01N 23/20025 - Sample holders or supports therefor
• G01N 23/207 - Diffractometry, e.g. using a probe in a central position and one or more displaceable detectors in circumferential positions
• G06T 17/00 - 3D modelling for computer graphics

Abstract

An AC-DC power converter includes: an AC rectification circuit configured to convert an AC line voltage into a rectified line voltage; a charge-pump-based power factor correction sub-converter configured to provide a DC-bus supply voltage; a resonant sub-converter configured to convert the DC-bus supply voltage to a DC output voltage or DC output current, the resonant sub-converter including: a resonant tank configured to convert the DC-bus supply voltage to a resonant voltage or current; and an output rectification circuit configured to generate the DC output voltage or DC output current from the resonant voltage or current to supply a converter load, and a controllable switch network shared by the charge-pump-based power factor correction sub-converter and the resonant sub-converter.

IPC Classes  ?

• H02M 1/42 - Circuits or arrangements for compensating for or adjusting power factor in converters or inverters
• H02M 3/07 - Conversion of DC power input into DC power output without intermediate conversion into AC by static converters using resistors or capacitors, e.g. potential divider using capacitors charged and discharged alternately by semiconductor devices with control electrode
• H02M 3/335 - Conversion of DC power input into DC power output with intermediate conversion into AC by static converters using discharge tubes with control electrode or semiconductor devices with control electrode to produce the intermediate AC using devices of a triode or a transistor type requiring continuous application of a control signal using semiconductor devices only

Abstract

The present invention relates to the in vitro use of a group of cell-penetrating conjugate systems or a formulation incorporating the same for transporting e.g. therapeutically active agents, such as chemotherapeutics, insulin and immunosuppressants across the cellular membranes of mammalian cells, including human cells. The invention also relates to an in vitro methods of transporting physiologically active agents, such as therapeutics, across cell-membranes by using the cell-penetrating conjugate systems according to the present invention as well as the cell-penetrating conjugate systems for use in treatment of cancer, diabetes and for use in immunotherapy.

IPC Classes  ?

• A61K 47/26 - Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharidesDerivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• A61K 47/18 - AminesAmidesUreasQuaternary ammonium compoundsAmino acidsOligopeptides having up to five amino acids
• A61K 31/4745 - QuinolinesIsoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having nitrogen as a ring hetero atom, e.g. phenanthrolines
• A61K 31/337 - Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
• A61K 31/282 - Platinum compounds
• A61P 35/00 - Antineoplastic agents

Abstract

A method for formation of monolithic nanostructures on an implantable device includes: a. depositing a metal film to a surface of the implantable device; b. heating the metal film for a period of time, such that the metal film transforms into multiple discrete nanoparticles, the multiple nanoparticles thereby forming an etch mask on the surface of the implantable device; c. etching the implantable device such that the surface of the implantable device is etched through the etch mask, thereby forming monolithic nanostructures in the surface of the implantable device; and d. (optionally) removing the etch mask, such as by immersion in an aqua regia solution.

IPC Classes  ?

• A61C 8/00 - Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereonDental implantsImplanting tools
• C23F 1/02 - Local etching

Abstract

A power converter assembly includes an interposer; an integrated circuit, such as a power management integrated circuit, arranged in a cavity or pocket of the interposer or monolithically integrated in the interposer; one or more electrical components stacked on a top side of the interposer; and one or more vias arranged in the interposer forming electrical connections in the interposer, wherein the integrated circuit and the electrical components are configured to perform a power conversion of an input voltage to an output voltage.

IPC Classes  ?

• H01L 23/538 - Arrangements for conducting electric current within the device in operation from one component to another the interconnection structure between a plurality of semiconductor chips being formed on, or in, insulating substrates
• H02M 3/00 - Conversion of DC power input into DC power output
• H01L 25/16 - Assemblies consisting of a plurality of individual semiconductor or other solid-state devices the devices being of types provided for in two or more different subclasses of , , , , or , e.g. forming hybrid circuits
• H01L 23/498 - Leads on insulating substrates
• H01L 23/053 - ContainersSeals characterised by the shape the container being a hollow construction and having an insulating base as a mounting for the semiconductor body

Abstract

The present disclosure relates to an ingestible device for sampling of the gastrointestinal tract. In particular, the device comprises an oral delivery capsule comprising a container having on the inside a hydrogel pre-polymer. The hydrogel prepolymer is capable of polymerising in situ, such as within the gastrointestinal tract, thereby collecting a sample of the gastrointestinal lumen by absorbing the lumen and its contents into the formed hydrogel.

IPC Classes  ?

• A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements

Abstract

The present invention relates to a lab-on-a-chip (LOAC)-system for the rapid detection of e.g. pathogens. The system comprises a tabletop detection apparatus and a portable optical detection cartridge for being received in the inner of the detection apparatus, the cartridges comprising a plurality of test wells for detecting a desired chemical reaction taking place within a respective test well. In embodiments of the invention, the optical detection cartridge is pre-loaded with suitable respective reagents selective for a disease pathogen such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 21/03 - Cuvette constructions
• G01N 21/64 - FluorescencePhosphorescence

Abstract

A permeabilized whole cell catalyst and method for conversion (reducing the amount) of a target substrate to one of more product are provided, wherein the catalyst is obtained by incubating the cells with a permeabilization agent selected from a fatty acid and fatty acid derivative, and wherein the permeabilized cells comprise an intracellular enzyme for catalyzing conversion of the target substrate. The target substrate transits through the channel, holes, or pore made in the cell membrane by the permeabilization agent, and is converted to products by enzymes retained within the cell.

IPC Classes  ?

• A23C 9/123 - Fermented milk preparationsTreatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceaeYoghurt
• A23C 9/12 - Fermented milk preparationsTreatment using microorganisms or enzymes
• A23L 2/60 - Sweeteners
• A23L 33/125 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing carbohydrate syrupsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugarsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugar alcoholsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing starch hydrolysates

Abstract

The present invention relates to detection of interactions between antigenic peptides and binding partners, such as T cell receptors (TCR) and antigenic peptide responsive T cells. In particular, the present invention relates to the provision of loadable detection molecules with peptide-free MHC molecules utilized for high throughput epitope identification and TCR specificity determination.

IPC Classes  ?

• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C12Q 1/6804 - Nucleic acid analysis using immunogens

Abstract

The present invention relates to artificial antigen presenting cell (aAPC) scaffolds to provide cells with specific functional stimulation to obtain phenotypic and functional properties ideal to mediate tumor regression or viral clearance. In particular, the scaffolds of the present invention comprise stabilized MHC class I molecules comprising a heavy chain comprising an alpha-1 domain and an alpha-2 domain connected by a disulfide bridge, wherein said MHC class I molecules are free of antigenic peptide. The scaffolds can be loaded with antigenic peptide on demand, providing an agile platform for effective expansion and functional stimulation of specific T cells in a peptide-MHC-directed fashion.

IPC Classes  ?

• C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
• A61P 35/00 - Antineoplastic agents
• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• A61P 17/00 - Drugs for dermatological disorders

Abstract

The invention provides a composition for use as a medicament, comprising cells of a recombinant microorganism capable of producing increased amounts of one or more of 5-hydroxytryptophan (5-HTP), 5-hydroxytryptamine (5-HT) and tryptamine (TRM) as compared to the non-recombinant microorganism from which it was derived. The composition finds use in preventing and/or treating TRM-; 5-HTP-, or 5-HT-related disorders of the central nerve system (CNS); enteric nervous system (ENS); gastro intestine (GI) and metabolism in a mammal, and may be orally administered to a mammal in need thereof. Additionally, a composition comprising cells of a recombinant microorganism capable of producing melatonin is provided for use as a medicament, such as for treatment of depression, dementia, cancer and sleep disorder.

IPC Classes  ?

• C12P 17/10 - Nitrogen as only ring hetero atom
• A61K 38/44 - Oxidoreductases (1)
• A61K 38/45 - Transferases (2)
• A61K 38/51 - Lyases (4)
• A61P 25/22 - Anxiolytics
• A61P 25/24 - Antidepressants
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 9/88 - Lyases (4.)

Abstract

The invention regards a method for electrochemical ammonia synthesis, comprising the steps of: providing at least one electrolysis cell; contacting the cathode with a source of lithium cations, nitrogen, and protons; and subjecting the cathode to a continuous pulsed cathode potential, including a pulsed cathodic current load, wherein the cathode potential is pulsed between the lithium reduction potential and a less negative cathode potential, whereby ammonia is synthesized.

IPC Classes  ?

• C25B 1/27 - Ammonia
• C01C 1/04 - Preparation of ammonia by synthesis
• C25B 9/15 - Flow-through cells
• C25B 15/02 - Process control or regulation

Abstract

The present invention relates to engineered Clustered Regularly Interspaced Short Palindromic Repeals (CRISPR) systems and corresponding guide RNAs that target specific nucleotide sequences at certain gene loci in the human genome. Also provided are methods of targeting, editing, and/or modifying of the human genes using the engineered CRISPR systems, and compositions and cells comprising the engineered CRISPR systems.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/90 - Stable introduction of foreign DNA into chromosome

Abstract

In certain embodiments a light therapy system (e.g., phototherapy device), such as for treatment of Alzheimer's disease, depression, dementia, short-term memory, or for improved learning, improved athletic performance or improved cognitive performance, is provided where the light system comprises a blue light source that operates at a frequency in the range from 20 to 50 Hz (preferably around 40 Hz), whereby the system enables retinal ganglion cells of a human to be exposed in order to stimulate brain waves (gamma oscillations in the human's brain).

IPC Classes  ?

• A61N 5/06 - Radiation therapy using light

Abstract

Methods for evolving cells or strains towards improved methyltransferase activity, particularly SAM-dependent methyltransferase activity, as well as to cells and strains useful in such methods and methods of using the evolved cells in the production of methylated products.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12N 1/36 - Adaptation or attenuation of cells
• C12N 9/88 - Lyases (4.)
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

a), sampling the vibration of the modified tension member to obtain a second vibration signal (VS2), and estimating the tension of the tension member based on a comparison of the first vibration signal (VS1) and the second vibration signal (VS2). Disclosed is also a system for estimating the tension of a tension member.

IPC Classes  ?

• G01L 5/04 - Apparatus for, or methods of, measuring force, work, mechanical power, or torque, specially adapted for specific purposes for measuring tension in flexible members, e.g. ropes, cables, wires, threads, belts or bands
• G01L 1/10 - Measuring force or stress, in general by measuring variations of frequency of stressed vibrating elements, e.g. of stressed strings
• G01M 7/02 - Vibration-testing

Abstract

A method and use of nisin-permeabilized microbial cells as whole-cell catalysts for reducing the amount of a target substrate in a sample to one of more product are provided. Specifically, a method of reducing the amount of lactose in a dairy sample using nisin-permeabilized lactic acid bacterial cell catalysts, which have been permeabilized by incubating with a nisin producing microbial cell and/or culture medium derived therof. Further provided is a nisin producing microbial cell, derived from parent strain Lactococcus lactis subsp. lactis bv. diacetylactis SD96 (NCBI accession No. SRX6686433).

IPC Classes  ?

• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12N 9/38 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase
• A23C 9/12 - Fermented milk preparationsTreatment using microorganisms or enzymes

Abstract

Disclosed is a pyrolysis system (1) comprising a pyrolysis reactor (2) arranged for producing pyrolysis gas and a first condensing unit (3) arranged to cool the pyrolysis gas to a first temperature to condense a first pyrolysis liquid (20). The system further comprises a second condensing unit (4) arranged to cool the pyrolysis gas to a second temperature to condense a second pyrolysis liquid (21), wherein the first temperature is higher than the second temperature. The system also comprises a return conduit (5) arranged to guide a portion of the pyrolysis gas back into the pyrolysis reactor (2) to drive the pyrolysis process, and heating means (6) arranged to increase the temperature of the portion of the pyrolysis gas before it reenters the pyrolysis reactor (2). The pyrolysis reactor (2) is a fixed bed counterflow pyrolysis reactor (2) comprising a pyrolysis gas outlet (7) arranged at an upper part (8) of the pyrolysis reactor (2) through which the produced pyrolysis gas leaves the pyrolysis reactor (2), a pyrolysis gas inlet (9) arranged at a lower part (10) of the pyrolysis reactor (2), through which the heated pyrolysis gas reenters the pyrolysis reactor (2), a feedstock inlet (11) arranged at the upper part (8) of the pyrolysis reactor (2) through which feedstock (19) enters the pyrolysis reactor (2) and a char outlet (12) arranged at the lower part (10) of the pyrolysis reactor (2), through which char produced in the pyrolysis reactor (2) leaves the pyrolysis reactor (2). Furthermore, a method for producing producing purified pyrolysis gas and pyrolysis liquids and use of a pyrolysis system (1) is disclosed.

IPC Classes  ?

• C10K 1/04 - Purifying combustible gases containing carbon monoxide by cooling to condense non-gaseous materials
• C10B 49/06 - Destructive distillation of solid carbonaceous materials by direct heating with heat-carrying agents including the partial combustion of the solid material to be treated with hot gases or vapours, e.g. hot gases obtained by partial combustion of the charge while moving the solid material to be treated according to the "moving bed" technique
• C10B 53/02 - Destructive distillation, specially adapted for particular solid raw materials or solid raw materials in special form of cellulose-containing material
• C10K 1/02 - Dust removal
• C10K 1/00 - Purifying combustible gases containing carbon monoxide
• C10K 3/00 - Modifying the chemical composition of combustible gases containing carbon monoxide to produce an improved fuel, e.g. one of different calorific value, which may be free from carbon monoxide

Abstract

A supramolecular conductive polymer composition comprising; a polymer comprising repeating subunits comprising at least one functional group, for forming a part in a hydrogen bond, selected from alcohol, amine, ether, amide, carboxylic acid, ester, ketone, nitrile, aldehyde and carbamide; a polyphenol comprising a minimum of 12 aromatic hydroxyl groups; a solvent with a dielectric constant of at least 20; and an electrically conductive filler.

IPC Classes  ?

• C08L 89/00 - Compositions of proteinsCompositions of derivatives thereof
• G01L 1/22 - Measuring force or stress, in general by measuring variations in ohmic resistance of solid materials or of electrically-conductive fluidsMeasuring force or stress, in general by making use of electrokinetic cells, i.e. liquid-containing cells wherein an electrical potential is produced or varied upon the application of stress using resistance strain gauges
• H01B 1/24 - Conductive material dispersed in non-conductive organic material the conductive material comprising carbon-silicon compounds, carbon, or silicon

Abstract

The present disclosure relates to methods for production of 4-hydroxytryptamine and derivatives thereof in a yeast cell. Herein are also disclosed methods for production of halogenated tryptophans and derivatives thereof in a cell. Herein are also disclosed methods for production of methylated tryptamine. The disclosure also provides nucleic acid constructs and cells useful for performing the present methods.

IPC Classes  ?

• C12P 13/22 - TryptophanTyrosinePhenylalanine3,4-Dihydroxyphenylalanine
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Abstract

A quantum random number generator includes an entropy source having a laser source, a single photodiode configured for generating photo current based on received light from the laser source, where the photodiode has a non-unity quantum efficiency for allowing interference of the light from the laser source with a vacuum state to obtain entropy from the vacuum state, a transimpedance amplifier to convert the photo current into voltage, an analog-to-digital converter for converting the analog voltage to a digital output, a security proof which establishes a lower bound on the entropy from the vacuum state, and a processing unit configured to convert the digital output from the analog-to-digital converter to random numbers based on the security proof.

IPC Classes  ?

• G06F 7/58 - Random or pseudo-random number generators

Abstract

The present invention relates to a cleavable linker platform. In particular, the invention relates to construction of an enzyme cleavable linker platform conjugated to a drug or a diagnostically relevant compound, a biomolecule, and an enzyme cleavable group, for which cleavage of the enzyme cleavable group leads to release of the drug or diagnostically relevant compound.

IPC Classes  ?

• A61K 47/65 - Peptidic linkers, binders or spacers, e.g. peptidic enzyme-labile linkers
• A61K 47/68 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
• A61P 35/00 - Antineoplastic agents

Abstract

A valve assembly using at least two pressure-sensitive leaky check valves has a non-linear flow-rate versus pressure-drop relation. A method for optimizing such a valve assembly is capable of determining a minimum required number of such valves and outputting the material parameters of each valve. The valve assembly and method allows for arbitrary flow control, which has numerous applications such as within drug delivery, food processing, and industrial flow control. The valve assembly is completely passive, i.e. there is no need for a feedback network. The flow control is achieved using only fluid-structure interactions.

IPC Classes  ?

• G05D 7/01 - Control of flow without auxiliary power
• G06F 30/28 - Design optimisation, verification or simulation using fluid dynamics, e.g. using Navier-Stokes equations or computational fluid dynamics [CFD]

Abstract

The present invention relates to immune stimulating micelle compositions, and their use in treatment of diseases and disorders, such as cancer. In particular, the present invention relates to micelle compositions comprising a TLR7 agonist, such as 1V270.

IPC Classes  ?

• A61K 47/00 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient
• A61K 8/02 - Cosmetics or similar toiletry preparations characterised by special physical form
• A61K 9/107 - Emulsions
• A61K 31/683 - Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
• A61K 31/704 - Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin, digitoxin
• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
• A61K 47/24 - Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
• A61P 35/00 - Antineoplastic agents
• C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
• C07K 16/32 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products from oncogenes

Abstract

The invention provides a genetically modified micro-organism for intracellular biosynthesis of a cellular metabolite, comprising a synthetic error correction system having a penalty gene, whose expression leads to arrested growth or cell death (e.g. a toxin gene) in combination with a survival gene, whose expression provides an antidote that restores cell viability and normal growth (e.g. a cognate antitoxin gene). Alternatively, the system has a survival gene, alone, whose expression is essential for growth (i.e. essential gene). The synthetic error correction system further comprises a biosensor, whose function is to induce expression of the survival gene which leads to cell growth, only, when the cell produces a pre-defined level of a given metabolite. The invention further encompasses: a method for producing the genetically modified micro-organism; a method for producing a cellular metabolite with the genetically modified micro-organism; and use of the genetically modified micro-organism for producing a cellular metabolite.

IPC Classes  ?

• C12P 7/42 - Hydroxy carboxylic acids
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/67 - General methods for enhancing the expression
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon
• C12P 1/02 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using fungi
• C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
• C12P 5/02 - Preparation of hydrocarbons acyclic
• C12P 7/40 - Preparation of oxygen-containing organic compounds containing a carboxyl group
• C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

An on-chip interferometer and a spectrometer including the interferometer are provided. An on-chip interferometer includes a waveguide for propagation of an optical signal including an input waveguide; at least two interferometer arms having one or more slot waveguides; and an output waveguide; wherein the input waveguide is split into the at least two interferometer arms which are recombined into the output waveguide; and a control mechanism configured for controlling a relative time delay between optical signals propagating in the two interferometer arms by modifying one or more slot widths of one or more of the slot waveguides; and wherein the relative time delay is at least 1, 2, 5, or at least 10 fs or at least one optical period of the longest optical wavelength of the optical signal.

IPC Classes  ?

• G01J 3/02 - SpectrometrySpectrophotometryMonochromatorsMeasuring colours Details
• G02F 1/225 - Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulatingNon-linear optics for the control of the intensity, phase, polarisation or colour by interference in an optical waveguide structure
• G01J 3/453 - Interferometric spectrometry by correlation of the amplitudes

Abstract

A magnetron plasma sputtering arrangement including an evacuable chamber, wherein in the evacuable chamber a tuning electrode, operatively connected to a biasing source with respect to ground, and including an aperture defining at least one axis of length, is arranged in a flow path for plasma between a sputtering head and a substrate. A plasma sputtered material originating at a sputtering target will traverse the aperture before depositing onto the surface of the substrate as a thin film.

IPC Classes  ?

• H01J 37/34 - Gas-filled discharge tubes operating with cathodic sputtering
• C23C 14/35 - Sputtering by application of a magnetic field, e.g. magnetron sputtering

Abstract

A method/system is provided for estimating distribution system conditions in a low-voltage network having local nodes, the local nodes including distributed energy resources. The method includes: acquiring periodic measurements of periodic node voltages and/or branch currents from nodes, the measurements acquired with fixed time intervals; acquiring event-driven measurements of event-driven data based on node voltages and/or branch currents from the energy resources and/or other event-driven data sources, the measurements acquired when a power operating point and/or other measured quantity changes exceed a predefined threshold; in a primary process, executing a distribution system state estimation based on a data set including the periodic measurements; in a secondary process, estimating impacts on the node voltages and/or branch currents in local nodes based on the event-driven measurements; wherein the estimated impacts on node voltages and/or branch currents in local nodes from the secondary process update the data set in the primary process.

IPC Classes  ?

• H02J 13/00 - Circuit arrangements for providing remote indication of network conditions, e.g. an instantaneous record of the open or closed condition of each circuitbreaker in the networkCircuit arrangements for providing remote control of switching means in a power distribution network, e.g. switching in and out of current consumers by using a pulse code signal carried by the network
• H02J 3/38 - Arrangements for parallelly feeding a single network by two or more generators, converters or transformers

Abstract

Systems and methods for assessment of one or more suspected conditions of an individual that include receiving collaborative identification data pertaining to features of a suspected condition and subject-specific phenomena of presentation, selecting target phenomena, receiving quantitative hypotheses for the target phenomena, defining an observation protocol comprising instructions for how the individual is to self-monitor and register observations by interaction with a portable data acquisition system, upon each occurrence of the target phenomena, directing the individual to provide the stored data upon observation of a target phenomenon during observation periods, transmitting the stored data from the portable data acquisition system to the analysis component, directing the individual to recall each observation period, completing one or more quantitative comparisons between any of the stored data, the quantitative hypotheses, and the recollection, inferring the suspected conditions, and determining a continued progress of the assessment.

IPC Classes  ?

• G16H 40/67 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for remote operation
• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients
• G16H 70/20 - ICT specially adapted for the handling or processing of medical references relating to practices or guidelines
• G16H 10/20 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for electronic clinical trials or questionnaires
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 20/70 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance relating to mental therapies, e.g. psychological therapy or autogenous training
• G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indicesICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
• H04L 12/58 - Message switching systems
• A61B 5/0205 - Simultaneously evaluating both cardiovascular conditions and different types of body conditions, e.g. heart and respiratory condition
• A61B 5/16 - Devices for psychotechnicsTesting reaction times

Abstract

An in-plane photonic device is provided for transmission of an optical signal across a gap, in particular an in-plane photonic device for use in a photonic integrated circuit with one or more in-plane crossings of electrical connections and photonic waveguides. One embodiment relates to an in-plane photonic device for use in a photonic integrated circuit with in-plane crossings of electrical connections and photonic waveguides, including: at least one input optical waveguide; and at least one output optical waveguide; wherein the at least one input optical waveguide and the at least one output optical waveguides are positioned such that a gap between them separates the input and the output optical waveguide(s), and wherein the input and the output optical waveguides are configured for optical mode matching across the gap, such that an optical signal can be transmitted from the input optical waveguide to the output optical waveguide across the gap.

IPC Classes  ?

• G02B 6/12 - Light guidesStructural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
• G02B 6/26 - Optical coupling means
• G02B 6/42 - Coupling light guides with opto-electronic elements
• G02B 6/14 - Mode converters

Abstract

The present invention relates to immunotoxins useful in treating diseases related to CMV infection. The invention also relates to use of the immunotoxin and pharmaceutical compositions comprising the immunotoxin as a medicament, and a kit for treatment or prevention of CMV infection comprising the immunotoxin.

IPC Classes  ?

• C07K 14/52 - CytokinesLymphokinesInterferons
• C07K 14/21 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Pseudomonadaceae (F)
• A61K 47/64 - Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
• A61P 31/12 - Antivirals

Abstract

Herein are provided microbial factories, in particular yeast factories, for production of strictosidine aglycone and optionally other plant-derived compounds. Also provided are methods for producing strictosidine aglycone in a microorganism, as well as useful nucleic acids, vectors and host cells.

IPC Classes  ?

• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 9/88 - Lyases (4.)
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)

Abstract

The present invention relates to microbial factories, in particular yeast factories, for production of ergothioneine. Also provided are methods for producing ergothioneine in a yeast cell, as well as useful nucleic acids, polypeptides, vectors and host cells.

IPC Classes  ?

• C12P 17/10 - Nitrogen as only ring hetero atom
• C07K 14/35 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Mycobacteriaceae (F)
• C12N 1/18 - Baker's yeastBrewer's yeast
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 9/88 - Lyases (4.)