e.ginter aliainter alia, compositions and methods for designing, constructing, testing, screening, growing, fermenting, and the like recombinant filamentous fungal strains expressing intein-modified fitness proteins.
Described herein, inter cilia, are glucoamylase variants and methods of using the same for saccharifying a starch substrate. Moreover, the disclosure also relates to a process of producing fermentation products and a method for increasing starch digestibility in an animal as well as a method of producing a fermented beverage using said as well as a. method of producing a fermented beverage using said glucoamylase variants.
The instant disclosure is generally related to recombinant polynucleotides comprising novel pro-region DNA sequences. Certain aspects of the disclosure are related to recombinant Gram-positive bacterial strains comprising one or more introduced polynucleotides comprising novel pro-region DNA sequences operably linked to DNA sequences encoding proteins of interest.
Disclosed herein are cellulase variants, or active fragments thereof, and polynucleotides encoding same, wherein the cellulase variants, or active fragments thereof, hav endoglucanase activity. Also disclosed herein are compositions comprising said cellulase variants, or active fragments thereof; vectors and/or host cells comprising the polynucleotides encoding said cellulase variants, or active fragments thereof; and methods for making and/or using said cellulase variants, or active fragments thereof and/or compositions containing same; wherein said cellulase variants, or active fragments thereof, have endoglucanase activity.
Certain aspects of the instant disclosure are related to the industrial scale production and recovery of lectin proteins. Certain aspects are related to recombinant Gram-positive bacterial cells producing heterologous lectin proteins. Certain other aspects are related to compositions and methods for recovering and/or purifying one or more lectins and enhanced purity lectin preparations thereof.
A recombinant yeast cell comprising a nucleotide sequence encoding a protein, which protein comprises an amino acid sequence of SEQ ID NO: 01 or an amino acid sequence which has at least 90% sequence identity, preferably at least 95%, 98%, or 99% sequence identity with the amino acid sequence of SEQ ID NO: 01.
Disclosed herein are compositions and methods for preventing, reducing, or removing malodor from liquid solutions, as well as from surfaces, such as fabrics, textiles or hard surfaces. Further disclosed herein are compositions and methods for preventing, reducing, or removing microbial growth from liquid solutions, as well as from surfaces, such as fabrics, textiles or hard surfaces.
C12N 9/36 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4 bonds between N-acetylmuramic acid and 2-acetylamino 2-deoxy-D-glucose, e.g. lysozyme
C11D 3/00 - Other compounding ingredients of detergent compositions covered in group
The present disclosure relates to serine proteases and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
Described are compositions and methods relating to yeast having a genetic mutation that results in decreased amounts of Cdc42 effector proteins, resulting in increased alcohol and lysine production. Such yeast is well-suited for use commercial alcohol production to increase yields and to increase the value of Such yeast is well-suited for use commercial alcohol production to increase yields and to increase the value of amino-acid-containing, fermentation-co-products.
Described are strains and methods relating to genetically-engineered yeast cells that overproduce lysine in a tunable manner by altering feedback inhibition of the lysine synthetic pathway by way of the LYS20 and LYS21 homocitrate synthase polypeptides. The yeast can be used in a conventional bioethanol production facility to produce alcohol along with increased amounts of lysine, resulting in increased quality and commercial value of fermentation products and co-products, such as animal feed ingredients.
inter aliaBacillusBacillusBacillus cells of the instant disclosure are particularly suitable for use in the expression, production and secretion of heterologous proteins.
Described herein is a method for producing complex branched isomalto-oligosaccharides (IMO) from maltodextrins. The method involves an enzyme having α-1,6-branching activity in combination with enzymes having transglucosidase and pullulanase activity to produce more complexly branched long-chain length IMO compared to IMO produced using a conventional method.
C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
A23L 29/30 - Foods or foodstuffs containing additivesPreparation or treatment thereof containing carbohydrate syrupsFoods or foodstuffs containing additivesPreparation or treatment thereof containing sugarsFoods or foodstuffs containing additivesPreparation or treatment thereof containing sugar alcohols, e.g. xylitolFoods or foodstuffs containing additivesPreparation or treatment thereof containing starch hydrolysates, e.g. dextrin
C07H 3/06 - Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
C12P 19/16 - Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Disclosed herein is a cleaning composition comprising at least one subtilisin variant and at least one surfactant, and a method for cleaning a surface with said cleaning composition.
The present disclosure relates to variant lipolytic enzymes, more particularly variant lipolytic enzymes that have improved stability and/or improved hydrolytic activity on a polyester. Such variant lipolytic enzymes find use in the degradation of polyesters, such as polyethylene terephthalate. Also provided are compositions and methods related to such variant lipolytic enzymes.
The present disclosure is generally related to Gram-positive bacterial strains comprising enhanced protein productivity phenotypes. Certain aspects are therefore related to compositions and methods for constructing recombinant (modified) Gram-positive bacterial strains for the enhanced production of proteins of interest.
inter aliainter alia, are compositions and methods for treating and/or preventing conditions associated with skin and/or oral inflammation via use of exogenously administered ATP degrading enzymes such as NTPDase enzymes of the GDA1_CD39 superfamily, or phosphatase enzymes that are capable of functioning along a physiologically relevant pH range.
This disclosure relates to a biodegradable core-shell microcapsule that includes (a) a microcapsule core comprising an active material; and (b) a microcapsule shell comprising a reaction product of an unfolded enzyme with a polyisocyanate; wherein the microcapsule shell is substantially free of or free of a self-condensed polyisocyanate, and the microcapsule shell has a biodegradation rate of at least 30%, 40%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, or 90%, based on the weight of the microcapsule shell, within 60 days according to OECD301F. This disclosure also relates to a liquid fragrance composition containing such biodegradable core-shell microcapsule. This disclosure also relates to a consumer product containing such biodegradable core-shell microcapsule. This disclosure also relates to a core-shell microcapsule slurry containing such biodegradable core-shell microcapsule. This disclosure also relates to a process for producing a core-shell microcapsule slurry.
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
The present invention relates to novel, stable forms of acetolactate decarboxylases (ALDCs) which have improved stability in the presence of proteases. More particularly, the present invention relates to an improved brewing process where stable ALDCs are used in conjunction with proline specific endoproteases during beer fermentation to provide a beer having less off-flavor which is colloidally stable in a shortened time.
Provided herein are compositions and methods relating to improved production of allulose by converting fructose to allulose using a allulose or psicose-3-epimerase from Brachyspira suanatina in the presence of base NaOH at 50 or 100mg/L.
Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Disclosed are compositions and methods involving proteases specific for mannose-modified proteins. The compositions and methods are particularly useful for making linker-specific cleavages in proteins produced by yeast and fungal cells. One use of the compositions and methods is for agglomerating yeast and yeast components in fermentation products. Another use of the composition is for producing a fraction of protein with reduced carbohydrate content.
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
Disclosed are compositions and methods relating to maltopentaose/maltohexaose-forming α-amylases. The variant α-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Described is a process for producing high quality starch hydrolysates using course-ground grains and seeds in a high temperature steeping process. The process is ideal for producing high quality starch hydrolysates in an energy-efficient manner, as well as for producing high quality animal feed and oil co-products.
The present disclosure is generally related to Gram-positive bacterial strains comprising enhanced protein productivity phenotypes. Certain aspects are therefore related to compositions and methods for constructing recombinant (modified) Gram-positive bacterial strains for the enhanced production of proteins of interest.
Described are compositions and methods relating to modified yeast that over-express cytochrome B2. The yeast produces an increased amount of alcohol compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates.
Described are compositions and methods relating to the over-expression of sugar transporter-like polypeptides to reduce the amount of glycerol and acetate produced by modified yeast having an exogenous pathway that cause it to produce more ethanol and acetate than its parental yeast.
Described herein, inter alia, are hybrid glucoamylase polypeptides derived from glucoamylases from Zygomycetes (e.g. Mucorales). Additionally, the disclosure also relates to processes for using the hybrid glucoamylase polypeptides disclosed herein for producing fermentation products as well as methods for increasing starch digestibility in an animal and in methods for producing fermented beverages.
Disclosed herein is one or more subtilisin variant useful for cleaning applications and in methods of cleaning. One embodiment is directed to one or more subtilisin variant, including one or more Bacillus sp. subtilisin polypeptide variant, and one or more cleaning composition comprising one or more such variant.
Disclosed are compositions and methods relating to engineered α-amylases. The engineered α-amy lases outperform commercial combinatoral variant α-amylases, which are currently the industry standard. The engineered α-amylases are useful for starch liquefaction and saccharification, and may also be useful for cleaning starchy stains, textile desizing, baking, and brewing.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
31 - Agricultural products; live animals
Goods & Services
Enzymes for industrial purposes; enzyme preparations for industrial purposes. Enzymes for veterinary purposes; enzyme preparations for veterinary purposes. Pet food; animal foodstuffs.
A method for preparing a dairy product having a stable content of GOS fiber, using a neutral lactase that will be inactive at pH below 4.7, and to a fermented GOS enriched milk-based product prepared by the method.
A23C 9/12 - Fermented milk preparationsTreatment using microorganisms or enzymes
C12N 9/38 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
A23C 21/02 - WheyWhey preparations containing, or treated with, microorganisms or enzymes
38.
RECOMBINANT FUNGAL CELLS AND METHODS THEREOF FOR INDUSTRIAL SCALE PRODUCTION OF LECTINS
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
A process for the production of ethanol. comprising: fermentation of a feed. under anaerobic conditions. wherein the feed contains a di-saccharide. oligo-saccharide and/or poly-saccharide and wherein the fermentation is carried out in the presence of a recombinant yeast cell. which recombinant yeast produces a combination of proteins having glucosidase activity: and recovery of ethanol. and a recombinant yeast cell for use therein.
A variant polypeptide of a parent polypeptide, wherein the parent polypeptide comprises the amino acid sequence of SEQ ID NO: 1, and wherein the variant polypeptide comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises an amino acid substitution of V202I, A203N, V333S, Y335M and/or D336G, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. A recombinant yeast cell functionally expressing a nucleotide encoding the variant polypeptide and a process for the production of ethanol using the variant polypeptide and/or recombinant yeast.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
PET FOOD; ANIMAL FOODSTUFFS ENZYMES FOR INDUSTRIAL PURPOSES; ENZYME PREPARATIONS FOR INDUSTRIAL PURPOSES ENZYMES FOR VETERINARY PURPOSES; ENZYME PREPARATIONS FOR VETERINARY PURPOSES
42.
USE AND PRODUCTION OF STORAGE-STABLE NEUTRAL METALLOPROTEASE
The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
A recombinant yeast cell functionally expressing: a) a nucleic acid sequence encoding a protein having NAD+-dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and b) a nucleic acid sequence encoding a protein having transketolase activity (EC 2.2.1.1), wherein the expression of the nucleic acid sequence encoding the protein having transketolase activity is under control of a promoter (the “TKL promoter”), which TKL promoter has an anaerobic/aerobic expression ratio for the transketolase of 2 or more.
Disclosed are compositions and methods relating to an improved hybrid lipase enzyme for reducing foaming in, for example, a carbohydrate fermentation process.
Disclosed herein are enzyme-containing granules, and compositions and methods related to the production and use thereof, including bioactive-containing granules that have improved features compared to one or more reference granules.
The present disclosure is generally related to recombinant fungal cells (strains) for use in the commercial scale production of proteins (polypeptides) of interest. Certain embodiments are therefore related to recombinant fungal cells (strains) producing proteins of interest and overexpressing one or more gene(s) encoding a regulatory protein of the disclosure.
Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more Bacillus gibsonii-clade subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
The present disclosure is generally related to recombinant microbial cells expressing heterologous proteins of interest. Certain aspects of the disclosure are therefore related to, inter alia, recombinant Bacillus cells having enhanced protein production capabilities, novel protein signal sequences, recombinant polynucleotides encoding heterologous proteins of interest, and related compositions and/or methods thereof. Thus, as exemplified herein, the recombinant Bacillus cells of the instant disclosure are particularly suitable for use in the expression, production and secretion of heterologous proteins.
Provided herein are animal diets containing phytase polypeptides or fragments thereof wherein the diet contains no or substantially no exogenously added trace minerals.
inter aliainter alia, are ruminant feed or feed additive compositions comprising one or more compounds that increase hydrogen production in a ruminant animal (such as, without limitation, 3-Nitrooxypropanol (3NOP)) and one or more acetogenic bacteria as well as methods for use of the same to reduce methane emissions in ruminant animals while simultaneously improving animal performance.
The instant disclosure provides, among other things, novel nuclease proteins (enzymes), novel nuclease compositions and protein preparations thereof, recombinant polynucleotides (DNA) encoding such nuclease proteins, recombinant host cells expressing and producing one or more nuclease proteins (optionally co-expressing one or more proteins of interest) and the like. More particularly, the nuclease proteins (enzymes) of the instant disclosure are particularly useful in mitigating DNA contamination, such as contaminating DNA present in a fermentation broth in which microbial host cells have been fermented, contaminating DNA present in recovered proteins of interest, contaminating DNA present in protein preparations, and the like.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Research and development of new products; research and development of new products for others in the field of bio-based materials; consulting services in the field of product design and development; consultancy services for others in the field of biotechnology, namely, bio-based materials; scientific and technological services, namely, research and development in the field of bio-based materials
The present compositions and methods relate to modified yeast cells that heterologously express the RuBisCo enzyme from a particular species of iron-oxidizing bacteria. The modified yeast cells demonstrate reduced glycerol and acetate accumulation in fermentation, while maintaining high ethanol production, making them useful for large-scale ethanol production from starch substrates, where glycerol and acetate represent undesirable by-products.
Disclosed herein are methods of producing a food product or food precursor. These methods can comprise: (a) providing a food product/precursor that comprises at least water and sucrose, and (b) contacting the food product/precursor with at least one glucosyltransferase enzyme (GTF) and, in some aspects, a second enzyme that utilizes sucrose as a substrate, such as an invertase. One or more GTFs can be selected from (i) a GTF that synthesizes alpha-1,6-glucan, and/or (ii) a GTF that synthesizes alpha-1,3-glucan. Such enzymatic treatment typically results in alpha-glucan production in the food product/precursor. Treatment of a food product/precursor with a second enzyme that utilizes sucrose as a substrate can modify the effects that the GTF treatment has on the food product/precursor, such as with texture formation, sugar composition, and/or sweetness. Food products and food precursors produced by this methodology are also disclosed.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Described are compositions and methods relating to modified yeast cells that over-express α-ketoglutarate dehydrogenase (K.GD2). Die modified yeast cells produce increased amounts of ethanol compared to otherwise identical parental yeast cells. Such yeast cells are particularly useful for large-scale ethanol production from starch substrates.
The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.
The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.
The present disclosure relates to serine proteases and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
C12N 9/54 - Proteinases derived from bacteria bacteria being Bacillus
C12N 15/70 - Vectors or expression systems specially adapted for E. coli
C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
C12N 15/76 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for ActinomycesVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Streptomyces
C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
The present disclosure relates to variant lipolytic enzymes, more particularly variant lipolytic enzymes that have improved stability and/or improved hydrolytic activity on a polyester. Such variant lipolytic enzymes find use in the degradation of polyesters, such as polyethylene terephthalate. Also provided are compositions and methods related to such variant lipolytic enzymes.
C12N 9/20 - Triglyceride splitting, e.g. by means of lipase
C08J 11/10 - Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
The invention relates to a recombinant cell, preferably a yeast cell comprising one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.
The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability compared to one or more reference subtilisin.
The present disclosure provides lipolytic enzyme variants having one or more modifications as compared to a parent lipolytic enzyme. Specifically, the present disclosure provides one or more lipolytic enzyme variants that has at least one improved performance when compared to one or more reference lipolytic enzymes. In addition, the present disclosure provides compositions comprising a lipolytic enzyme variant of the disclosure. The present disclosure also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the disclosure.
Certain embodiments of the disclosure are related to recombinant Bacillus strains comprising enhanced protein productivity phenotypes, compositions and methods for constructing such recombinant Bacillus cells, and the like. More particularly, the recombinant Bacillus strains described herein are particularly useful for the enhanced production of proteins of interest when grown/cultivated/fermented under suitable conditions.
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
The present invention relates to improved techniques for brewing. More particularly, the present invention relates to compositions and methods for improving mash filterability and yield.
The present invention relates to the production of rye based spirits using enzymes. More particularly, the present invention relates to methods of mashing rye grists using xylanases that have reduced sensitivity to rye XIP inhibitor.
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
MATERIAL TESTING INSTRUMENTS AND MACHINES, NAMELY, LATERAL FLOW TEST KITS; MEASURING APPARATUS, NAMELY, NEAR-INFARED SPECTROSCOPY (NIR) TECHNOLOGY TO MEASURE ENERGY VALUES IN PLANTS PROVIDING VIRTUAL COMPUTER SYSTEMS THROUGH CLOUD COMPUTING; PROVIDING SCIENTIFIC INFORMATION, ADVICE AND CONSULTANCY RELATING TO ANIMAL NUTRITION; TECHNOLOGICAL ANALYSIS AND CONSULTANCY IN THE FIELD OF ANIMAL NUTRITION
Aspects of the present compositions and methods relate to novel metalloproteases polynucleotides encoding the novel metalloprotease, compositions and methods for use thereof.
Described is a method for increasing the amounts of fermentable sugars in fermentation substrates by treatment with a combination of an enzyme having transglucosidase activity and an enzyme having glucoamylase activity to hydrolyze oligo and/or polysaccharides that are not conventionally hydrolyzed by glucoamylase alone during fermentation. The method is most effective using fermentation substrates containing low amounts of maltose and maltotriose.
The invention relates to a process for the production of ethanol from a composition comprising at least glucose comprising fermenting said composition in the presence of a recombinant yeast; and recovering the ethanol, wherein said yeast comprises one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding for an enzyme having dihydroxyacetone kinase activity (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter, wherein the composition comprises an amount of undissociated acetic acid of 10 mM or less. A recombinant yeast having the genes as described above is particularly sensitive towards acetic acid, and the ethanol yield rapidly decreases when the composition contains more than 10 mM undissociated acetic acid.
The present invention relates to a method or treating plant proteins with enzymes to produce a plant-based food, including plant-based meat alternatives. More particularly, the method relates to use of transglutaminase, a sugar oxidase, and catalase to treat plant-based proteins.
inter aliae.getcetc.) in modified filamentous fungal cells, methods and compositions for producing proteins of interest in recombinant filamentous fungal, wherein the proteins produced and secreted into the broth and/or proteins recovered from the broth have uniform and consistent N-linked glycosylation patterns, and/or reduced (unwanted) glycation of one or more proteins of interest, and the like.
The present invention relates to the use of a fungal protein to create a food product. More particularly, the present invention relates to the use of an overexpressed native polypeptide to create a food product.
inter aliainter alia, methods for remediating biuret in aqueous liquids/solutions, methods for remediating cyanuric acid and biuret in aqueous liquids/solutions. Certain one or more embodiments or aspects of the disclosure are directed to methods for remediating biuret in freshwater swimming pools, saltwater swimming pools and the like.
42 - Scientific, technological and industrial services, research and design
Goods & Services
RESEARCH AND DEVELOPMENT OF NEW PRODUCTS; RESEARCH AND DEVELOPMENT OF NEW PRODUCTS FOR OTHERS IN THE FIELD OF BIO-BASED MATERIALS; CONSULTING SERVICES IN THE FIELD OF PRODUCT DESIGN AND DEVELOPMENT; CONSULTANCY SERVICES FOR OTHERS IN THE FIELD OF THE RESEARCH OF BIO-BASED MATERIALS; SCIENTIFIC AND TECHNOLOGICAL SERVICES, NAMELY, RESEARCH AND DEVELOPMENT IN THE FIELD OF BIO-BASED MATERIALS.
42 - Scientific, technological and industrial services, research and design
Goods & Services
RESEARCH AND DEVELOPMENT OF NEW PRODUCTS; RESEARCH AND DEVELOPMENT OF NEW PRODUCTS FOR OTHERS IN THE FIELD OF BIO-BASED MATERIALS; CONSULTING SERVICES IN THE FIELD OF PRODUCT DESIGN AND DEVELOPMENT; CONSULTANCY SERVICES FOR OTHERS IN THE FIELD OF THE RESEARCH OF BIO-BASED MATERIALS; SCIENTIFIC AND TECHNOLOGICAL SERVICES, NAMELY, RESEARCH AND DEVELOPMENT IN THE FIELD OF BIO-BASED MATERIALS.
The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) of a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grown on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
The present invention provides serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
Described are compositions and methods relating to modified yeast with disrupted genes that produces an increased amount of ethanol and, in some cases, a decreased amount of acetate compared to otherwise identical parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates with high dissolved solids.
Disclosed herein is one or more subtilisin variants, nucleic acids encoding same, and compositions and methods related to the production and uses thereof, including one or more subtilisin variants that has improved stability and/or soil removal compared to one or more reference subtilisin.
The disclosure relates to the field of molecular biology, to compositions and methods for the usage of selection marker swapping systems in microbial cells. Specifically, this disclosure pertains to compositions and methods for swapping between two selection marker constructs at a predetermined target sequence within a microbial genome, by replacing a first removable selection marker construct with a second removable selection marker construct, followed by the reverse replacement in consecutive transformation steps. Methods and compositions are also disclosed in which selection marker swapping systems are used in multiplex genome engineering, by combining selection marker swapping with simultaneously modifying at least one additional target sequence at a different genome sequence.
The present disclosure is generally related to compositions and methods for obtaining Bacillus licheniformis cells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modified Bacillus licheniformis cells/strains derived from parental B. licheniformis cells/strains comprising a variant rghR2 gene.
The disclosure relates to the field of molecular biology, to compositions and methods for the usage of recombinant self-excisable selection marker systems in microbial cells. Specifically, this disclosure pertains to compositions and methods for reestablishing a predetermined target sequence within a microbial genome, by integrating and excising two recombinant self-excisable selection marker constructs in consecutive transformation steps. Methods and compositions are also disclosed in which recombinant self-excisable selection marker systems are used in multiplex genome engineering, by combining marker integration with simultaneously modifying at least one additional target sequence at a different genome sequence.
The disclosure relates to the field of molecular biology, to compositions and methods for the usage of bidirectional selection marker systems in microbial cells. Specifically, this disclosure pertains to compositions and methods for reestablishing a predetermined target sequence within a microbial genome, by integrating and excising a bidirectional selection marker construct in consecutive transformation steps. Methods and compositions are also disclosed in which bidirectional selection marker systems are used in multiplex genome engineering, by combining marker integration or excision with simultaneously modifying at least one additional target sequence at a different genome sequence.
Described herein is at least one novel trypsin-like serine protease polypeptide and uses thereof. Further described herein are cleaning compositions containing at least one polypeptide described herein, wherein said composition can be used to clean fabrics and hard surfaces. Even further described herein is at least one cleaning composition selected from a laundry detergent, a dishwashing detergent (e.g., automatic and hand dish), and a personal care composition. Even still further, at least one polypeptide having improved soil removal and/or stability compared to at least one reference polypeptide is described herein.
inter aliae.ge.g., mitigating/reducing bioreactor cooling needs and reducing operating costs) and the like, methods and compositions for cultivating/fermenting filamentous fungal strains at increased temperature ranges for the production of proteins of interest and the like.
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
The present disclosure relates to variant lipolytic enzymes, more particularly variant lipolytic enzymes that have improved stability and/or improved hydrolytic activity on a polyester. Such variant lipolytic enzymes find use in the degradation of polyesters, such as polyethylene terephthalate. Also provided are compositions and methods related to such variant lipolytic enzymes.
Disclosed herein are methods of glucosylating an isoflavone glycoside, as well as methods of reducing off-flavor in a food product/precursor. Further disclosed are compositions comprising one or more glucosylated isoflavone glycosides, such as a food product/precursor.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/44 - Preparation of O-glycosides, e.g. glucosides
A23L 27/00 - SpicesFlavouring agents or condimentsArtificial sweetening agentsTable saltsDietetic salt substitutesPreparation or treatment thereof
A23L 29/00 - Foods or foodstuffs containing additivesPreparation or treatment thereof
98.
COMPOSITIONS FOR CLEANING AND METHODS RELATED THERETO
Disclosed herein are cleaning compositions comprising renewable components. More particularly, the cleaning compositions comprise an organic acid derivative of mono- and diglycerides, a functionalized polymer, an enzyme system, and a polar protic solvent other than water.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Providing scientific information, advice and consultancy relating to process optimization and product development for manufacturers of sugar syrups; technological consultancy; scientific and technological research relating to process optimization and product development for manufacturers of sugar syrups.
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Providing scientific information, advice and consultancy relating to process optimization and product development for manufacturers of sugar syrups; advisory and consultancy services in the field of grain processing; providing scientific research and technical advice relating to process optimization and product development for manufacturers of sugar syrups; scientific and technological research in the field of grain processing; providing technological consultancy, research and development services in the field of process optimization and product development for manufacturers of sugar syrups; providing technology consultancy in the field of grain processing.
(2) Product design consulting services
