In accordance with one embodiment, a method comprises exciting differing fluorochromes to fluorescence that mark a plurality of moving cells; collecting a broadband light signal having a broadband wavelength range from the fluorescence of the differing fluorochromes; splitting out wavelength ranges in the broadband light signal into smaller wavelength ranges in light signal paths; separately detecting, with differing photo detectors, the light signals in each of the smaller wavelength ranges in the light signal paths; generating digital signals, with analog to digital converters, from the detected light signals in the smaller wavelength ranges in the light signal paths; aligning the digital signals over the broadband wavelength range; and combining the aligned digital signals together into a full spectral response based on the broadband light signal having the broadband wavelength range.
In one embodiment, a system includes a flow cell assembly having a flow cell body with a base having an opening allowing sheathed sample fluid to flow out, a bracket coupled to the flow cell body, a flat mirror mounted to the bracket, a hardware triggered camera coupled to the bracket on one side, and a diffused light emitting diode strobe light coupled to the bracket on an opposite side. The camera and strobe light are pointed at the mirror on opposite sides. Activation of the diffused LED strobe light generates a diffused strobe light into the mirror backlighting a droplet stream. Triggering of the camera is synchronized with the generation of the strobe light to periodically capture a brightfield still image of the droplet stream. The image can be analyzed for measured jet breakoff point and measured droplet interval point for visual feedback control of the droplet stream.
G06V 10/34 - Smoothing or thinning of the patternMorphological operationsSkeletonisation
G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersectionsConnectivity analysis, e.g. of connected components
G06V 10/762 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using clustering, e.g. of similar faces in social networks
In accordance with one embodiment, a method comprises exciting differing fluorochromes to fluorescence that mark a plurality of moving cells; collecting a broadband light signal having a broadband wavelength range from the fluorescence of the differing fluorochromes; splitting out wavelength ranges in the broadband light signal into smaller wavelength ranges in light signal paths; separately detecting, with differing photo detectors, the light signals in each of the smaller wavelength ranges in the light signal paths; generating digital signals, with analog to digital converters, from the detected light signals in the smaller wavelength ranges in the light signal paths; aligning the digital signals over the broadband wavelength range; and combining the aligned digital signals together into a full spectral response based on the broadband light signal having the broadband wavelength range.
G02B 27/09 - Beam shaping, e.g. changing the cross-sectioned area, not otherwise provided for
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
G02B 7/04 - Mountings, adjusting means, or light-tight connections, for optical elements for lenses with mechanism for focusing or varying magnification
H10F 30/225 - Individual radiation-sensitive semiconductor devices in which radiation controls the flow of current through the devices, e.g. photodetectors the devices having potential barriers, e.g. phototransistors the devices being sensitive to infrared, visible or ultraviolet radiation the devices having only one potential barrier, e.g. photodiodes the potential barrier working in avalanche mode, e.g. avalanche photodiodes
G02B 27/14 - Beam splitting or combining systems operating by reflection only
A multispectral imaging flow cytometer acquires a variety of images in different imaging modes, such as brightfield, side scatter, and a plurality of fluorescent images of a different moving biological cells in a sample fluid. These images can be processed by a plurality of artificial intelligence algorithms and/or machine learning tools executed by a processor, a neural engine, a neural processor, or a convolutional neural network (CNN). Deep learning analysis of the images can be performed with the CNN on the images to extract image features. Feature data can be extracted about the moving biological cell as well. An AI algorithm, such as random forest algorithm, can use both the image features of a cell and the feature data of the cell to classify the biological cell as to its type.
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
G06V 10/40 - Extraction of image or video features
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
A flow cytometer or cell sorting system includes a linear array detector having a plurality of detectors; a plurality of low noise gain amplifiers respectively coupled to the plurality of detectors in the linear array detector; a plurality of analog to digital converters respectively coupled to the plurality of detectors of the linear array detector; and image reconstruction logic coupled to the plurality of analog to digital converters. The linear array detector receives the brightfield image of each cell of the plurality of biological cells and transduces the light into analog signals representative of pixels in a plurality of brightfield image lines of each cell. The plurality of analog to digital converters transduce the analog signals into digital numeric signals for each pixel. The image reconstruction logic reconstructs the plurality of brightfield image lines of each cell over time periods into a single overall brightfield image of each cell.
A user interface wizard generated by AI software walks a user through setting up an artificial intelligence (AI) image analysis experiments on multispectral cellular images using AI image analysis algorithms and AI feature analysis algorithms. In a training experiment mode, a new AI model a new AI model can be trained for a desired experiment on training image cellular data of biological cells in a sample and subsequently to run a classification experiment in a classification mode to classify new image cellular data of biological cells in the sample using the new AI model.
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
G06F 9/451 - Execution arrangements for user interfaces
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
7.
COMBINING BRIGHTFIELD AND FLUORESCENT CHANNELS FOR CELL IMAGE SEGMENTATION AND MORPHOLOGICAL ANALYSIS IN IMAGES OBTAINED FROM AN IMAGING FLOW CYTOMETER
A classifier engine provides cell morphology identification and cell classification in computer-automated systems, methods and diagnostic tools. The classifier engine performs multispectral segmentation of thousands of cellular images acquired by a multispectral imaging flow cytometer. As a function of imaging mode, different ones of the images provide different segmentation masks for cells and subcellular parts. Using the segmentation masks, the classifier engine iteratively optimizes model fitting of different cellular parts. The resulting improved image data has increased accuracy of location of cell parts in an image and enables detection of complex cell morphologies in the image. The classifier engine provides automated ranking and selection of most discriminative shape based features for classifying cell types.
In one embodiment, a method to synthesize highly fluorescent complexes is disclosed. The highly fluorescent complexes are synthesized by using inorganic nanoparticles, coupling agents, linkers, and fluorescent dye molecules. The unique nanoparticle-dye complexes (referred to as “SN-dye”) can provide ultra-bright fluorescent labelling. This is demonstrated by coupling the complexes to the antibody and subsequently using the conjugated antibody for more sensitive immunological detection in flow cytometry applications.
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
In one embodiment, a method to synthesize highly fluorescent complexes is disclosed. The highly fluorescent complexes are synthesized by using inorganic nanoparticles, coupling agents, linkers, and fluorescent dye molecules. The unique nanoparticle-dye complexes (referred to as "SN-dye") can provide ultra-bright fluorescent labelling. This is demonstrated by coupling the complexes to the antibody and subsequently using the conjugated antibody for more sensitive immunological detection in flow cytometry applications.
Methods for a sorting flow cytometer system are disclosed. The method includes forming a stream of a sample biological fluid; charging the stream of the sample biological fluid with a first voltage having a first voltage polarity; breaking a first charged drop off of the stream, wherein the first charged drop is charged with the first voltage; dropping the first charged drop between a first electrostatic charge plate and a second electrostatic charge plate opposite the first electrostatic charge plate, wherein the first electrostatic charge plate and the second electrostatic charge plate are positioned on an angle to form a progressively larger gap between each; deflecting the first charged drop off of a center stream path towards the first electrostatic charge plate to drop along a line with a first angle; and orienting a first pivotal scupper to allow the first charged drop to fall into a first test tube.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
A pressure regulated fluidics system of a flow cytometer includes a sheath tank holding sheath fluid; a degasser coupled to the sheath tank by a first sheath line; a manifold assembly coupled to the degasser by a second sheath line; a first valve coupled to the second sheath line; a pressure regulator coupled to the sheath tank; and a transducer coupled to and between the pressure regulator and the first valve. The transducer senses measured pressure and converts it into a voltage. The pressure regulator applies regulated pressure to the sheath fluid to maintain a total flow rate of fluids through the flow cytometer based on the voltage. The degasser pulls gas molecules out of the sheath fluid.
09 - Scientific and electric apparatus and instruments
Goods & Services
Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, flow cytometers; Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, cell sorters; Medical laboratory research instruments for analyzing biological cells and particles; Recorded software for the operation of laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses; and Downloadable computer software and software recorded on computer media for clinical and laboratory instruments for analyzing biological fluids;
42 - Scientific, technological and industrial services, research and design
Goods & Services
Cloud computing featuring software for use in the field of biological science; and Providing temporary use of on-line non-downloadable cloud computing software for preparation of biological science experiments.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Biochemical reagents used for non-medical purposes; Laboratory chemicals, namely, an antibody reagent used for the detection of antigens in cell and tissue analysis for scientific in vitro diagnostic use; Reagents for scientific and research use; Reagents for biological analysis for scientific use; Biochemical reagents, other than for medical or veterinary purposes, for detecting and analyzing molecules in protein or nucleotide arrays; Chemical reagents, other than for medical or veterinary purposes; Diagnostic reagents for scientific or research use; Diagnostic reagents for laboratory use, other than for medical or veterinary purposes; Biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; Fluorescent dye for scientific or research use; and Chemicals, namely, buffer and standard solutions used in analytical chemistry. Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, flow cytometers; Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, cell sorters; Medical laboratory research instruments for analyzing biological cells and particles; and Recorded software for the operation of laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses. Cloud computing featuring software for use in preparing experiments in the field of biological science; and Providing temporary use of on-line non-downloadable cloud computing software for preparation of biological science experiments.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Biochemical reagents used for non-medical purposes; Laboratory chemicals, namely, an antibody reagent used for the detection of antigens in cell and tissue analysis for scientific in vitro diagnostic use; Reagents for scientific and research use; Reagents for biological analysis for scientific ues; Biochemical reagents, other than for medical and veterinary purposes, for detecting and analyzing molecules in protein or nucleotide arrays; Chemical reagents, other than for medical or veterinary purposes; Diagnostic reagents for scientific or research use; Diagnostic reagents for laboratory use, other than for medical and veterinary purposes; Biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; Fluorescent dye for scientific or research use; and Chemicals, namely, buffer and standard solutions used in analytical chemistry. Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, flow cytometers; Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, cell sorters; Medical laboratory research instruments for analyzing biological cells and particles; and Recorded software for the operation of laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses. Cloud computing featuring software for use in preparing experiments in the field of biological science; and Providing temporary use of on-line non-downloadable cloud computing software for preparation of biological science experiments.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Biochemicals for in vitro and in vivo scientific use; Buffer solutions for use in analytical chemistry; Reagents for scientific and research use; Reagents for scientific or medical research use; Antibody reagents used for the detection of antigens in cells and tissue, other than for medical or veterinary purposes; Biochemical reagents used for non-medical purposes; Biochemical reagents, other than for medical or veterinary purposes; Diagnostic reagents for scientific or research use; Diagnostic reagents, other than for medical or veterinary purposes; Monoclonal antibodies for scientific purposes
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Biochemicals for in vitro and in vivo scientific use; Buffer solutions for use in analytical chemistry; Reagents for scientific and research use; Reagents for scientific or medical research use; Antibody reagents used for the detection of antigens in cells and tissue, other than for medical or veterinary purposes; Biochemical reagents used for non-medical purposes; Biochemical reagents, other than for medical or veterinary purposes; Diagnostic reagents for scientific or research use; Diagnostic reagents, other than for medical or veterinary purposes; Monoclonal antibodies for scientific purposes
A circular nozzle assembly is disclosed. A cuvette nozzle subsystem (assembly) for flow cytometry systems is disclosed including a cuvette assembly and a circular nozzle assembly selectively engaged with the cuvette assembly. The cuvette assembly includes a cuvette having a pocket and a flow channel, and a receptacle coupled within the pocket to the cuvette. The receptacle has a through-hole with a tapered conical portion and a circular cylindrical portion. The circular nozzle assembly includes an o-ring gasket coupled to a nozzle body with a flow channel. A tapered conical portion of the nozzle body engages the tapered conical portion of the through-hole to align the respective flow channels of the cuvette and the nozzle body together.
A smart flow cytometer is disclosed including a plurality of tanks holding fluids for operation of a flow cytometer; a plurality of level sensors respectively coupled to the plurality of tanks; a plurality of valves coupled to one or more tubes for operation of the flow cytometer; one or more flow sensors coupled to the one or more tubes for monitoring flow rates; and a microcontroller coupled to the plurality of level sensors and the one or more flow sensors. The microcontroller constantly monitors fluid levels of the plurality of tanks to detect when to refill a fluid in a first tank before the first tank is completely empty and to detect when to empty a second tank before the second tank becomes completely filled with a waste fluid.
A method for a flow cytometer includes generating at least one light output with at least one excitation light source; carrying a bio-sample in a medium to a region of a flow path, wherein the bio-sample in the region is illuminated by the at least one light output of the at least one excitation light source to give off luminescence; spatially dispersing a spectrum of the luminescence across photo detection inputs of a photo-detector array; receiving eight or more analog signals from the photo-detector array and converting them to eight or more digital signals respectively; generating a self trigger signal based on the eight or more digital signals and the luminescence; and causing a digital storage space and a digital processing unit to respectively store and analyze the digital data of the eight or more digital signals based on the self trigger signal.
A circular nozzle assembly is disclosed. A cuvette nozzle subsystem (assembly) for flow cytometry systems is disclosed including a cuvette assembly and a circular nozzle assembly selectively engaged with the cuvette assembly. The cuvette assembly includes a cuvette having a pocket and a flow channel, and a receptacle coupled within the pocket to the cuvette. The receptacle has a through-hole with a tapered conical portion and a circular cylindrical portion. The circular nozzle assembly includes an o-ring gasket coupled to a nozzle body with a flow channel. A tapered conical portion of the nozzle body engages the tapered conical portion of the through-hole to align the respective flow channels of the cuvette and the nozzle body together.
G01N 21/78 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
24.
FLOW CYTOMETERY SYSTEM WITH FLUIDICS CONTROL SYSTEM
A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.
A flow cell subassembly includes a carriage assembly coupled to a flow cell body to selectively engage a nozzle with a base of a cuvette. The carriage assembly includes a linear bearing slidingly engaged with the flow cell body, a tiltable carriage plate coupled to the linear bearing, and a nozzle mount coupled to the tiltable carriage plate. The nozzle mount receives a nozzle assembly with the nozzle. Set screws can adjust pitch angle of the tiltable carriage plate with the linear bearing to adjust engagement between the nozzle and cuvette in a first dimension. To adjust engagement between the nozzle and cuvette in a second dimension, axial play in bolts/screws through the tiltable carriage plate into threaded holes of the linear bearing allow for yaw angle adjustments.
In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a spectral flow cytometer with a least three excitation lasers and thirty-eight color detectors. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
09 - Scientific and electric apparatus and instruments
Goods & Services
Computer hardware with preinstalled software for the control of scientific instruments and laboratory equipment for scientific, laboratory, and general research purposes; Downloadable computer programs for the control of scientific instruments and laboratory equipment for scientific, laboratory, and general research purposes; Downloadable computer firmware for the control of scientific instruments and laboratory equipment for scientific, laboratory, and general research purposes; Downloadable computer software for the control of scientific instruments and laboratory equipment for scientific, laboratory, and general research purposes; Recorded computer programs for the control of scientific instruments and laboratory equipment for scientific, laboratory, and general research purposes; Recorded computer firmware for the control of scientific instruments and laboratory equipment for scientific, laboratory, and general research purposes; Recorded computer software and hardware for the control of scientific instruments and laboratory equipment for scientific, laboratory, and general research purposes sold as a unit; Recorded computer software for the control of scientific instruments and laboratory equipment for scientific, laboratory, and general research purposes
28.
METHODS AND APPARATUS FOR A TWENTY-FIVE-COLOR FLUORESCENCE-BASED ASSAY AND FLOW CYTOMETRY PANEL
In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with four excitation lasers and forty-eight color detectors. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Cloud computing featuring software for use in preparing experiments in the field of biological science; Providing temporary use of on-line non-downloadable cloud computing software for preparation of biological science experiments
30.
METHODS FOR DETECTING FLUORESCENT LIGHT WITH DE-MULTIPLEXING IMAGING ARRAYS OF A COMPACT DETECTION MODULE IN A FLOW CYTOMETER
A method includes launching, from an optical fiber, fluorescent light of differing wavelengths generated by different fluorochromes attached to particles in a sample fluid; magnifying an image size from an end of the optical fiber to a first dichroic filter of a row of a plurality of dichroic filters in a de-multiplexing imaging array; alternatively reflecting the fluorescent light between the plurality of dichroic filters and a plurality of micro-mirrors to collimate the fluorescent light on odd numbered dichroic filters and re-image the fluorescent light on even numbered dichroic filters; band passing different wavelength ranges of the fluorescent light at each of the plurality of dichroic filters to de-multiplex the wavelength spectrum of the wavelength range of the fluorescent light; and detecting fluorescent light in each of the different wavelength ranges to count a number of each of the different particles in the sample fluid.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; Laboratory chemicals, namely, an antibody reagent used for the detection of antigens in cell and tissue analysis for in vitro diagnostic use
32.
METHODS, APPARATUS, AND SYSTEMS FOR AN OPTICAL FIBER FORWARD SCATTER CHANNEL IN FLOW CYTOMETERS
An optical fiber forward scatter channel in a flow cytometer is disclosed. A detector system in the flow cytometer includes fiber optic cable for receiving scattered light from an incident laser light that is directed at cells/particles passing through the flow cytometer. The fiber optic cable delivers the scattered light to a sensor system, which collects data to perform analyses on the scattered light. Such analyses may include, for example, calculating the size of a cell/particle, counting cells/particles, and so on. The fiber optic cable is an inherently efficient and accurate filter for the acceptance or rejection of the scattered light.
09 - Scientific and electric apparatus and instruments
Goods & Services
Automated test tube reagent mixture preparation apparatus for scientific, laboratory, and general research purposes; Automated test tube chemical mixture preparation instrument for scientific, laboratory, and general research purposes; and Automated robotic test tube mix-making equipment for scientific, laboratory, and general research purposes
A compact sorting flow cytometer system is disclosed. The system includes a flow cell and a deflection unit in communication with the flow cell to receive charged drops and uncharged drops in a stream of a sample biological fluid. The deflection unit includes a case having a deflection cone and chamber, a tub with a drain, and a slot in a base. The deflection unit further includes electrostatic charge plates in the cone forming an electrostatic charge field; and first and second pivotal scuppers, and a center non-pivotal collector arranged along a shaft in the tub of the case. Uncharged drops pass the electrostatic charge field undeflected in a center stream path while charged drops are deflected away from it. The pivotal scuppers pivot between positions to direct charged drops into the tub for aspiration or to pass through the slot in the base for collection.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
09 - Scientific and electric apparatus and instruments
Goods & Services
Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, flow cytometers and cell sorters; and Medical laboratory research instruments for analyzing biological cells and particles
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, flow cytometers; and Medical laboratory research instruments for analyzing biological cells and particles in a stream of drops Medical instruments, apparatus, and equipment for medical diagnostic uses, namely, flow cytometers
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Laboratory instruments, apparatus, and equipment for scientific, laboratory, and general research uses, namely, flow cytometers; and Medical laboratory research instruments for analyzing biological cells and particles in a stream of drops Medical instruments, apparatus, and equipment for medical diagnostic uses, namely, flow cytometers
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Antibody reagents for scientific and research use in the field of flow cytometry with flow cytometers; Diagnostic antibody reagents for clinical or medical laboratory use in the field of flow cytometry with flow cytometers
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Biochemicals for in vitro and in vivo scientific use; Diagnostic reagents for clinical or medical laboratory use; Reagents for scientific and research use
A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometer or cell sorter system includes a fluidics system and a flow cell. The fluidics system is under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes a drop drive assembly, a flow cell body, and a cuvette coupled together. The drop drive assembly includes a sample injection tube (SIT) in communication with the fluidics system to receive sample fluid. The flow cell body receives the sample fluid from the sample injection tube and sheath fluid. The flow cell body has a charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; and a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein.
A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometer or cell sorter system includes a fluidics system and a flow cell. The fluidics system is under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes a drop drive assembly, a flow cell body, and a cuvette coupled together. The drop drive assembly includes a sample injection tube (SIT) in communication with the fluidics system to receive sample fluid. The flow cell body receives the sample fluid from the sample injection tube and sheath fluid. The flow cell body has a charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; and a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein.
A flow cell body for a flow cytometer or a cell sorter is provided. The flow cell body comprises the following: a three-dimensional opaque (e.g., black) polymer body having top, bottom, left, right, front, and back sides. The opaque polymer body includes the following: a top side opening into a chamber to receive a drop drive assembly including a sample injection tube (SIT), wherein the chamber has an upper circular cylindrical portion and a lower funnel portion; two or more opposing top side openings receiving threaded inserts to engage two or more threaded bolts to hold a hub of the drop drive assembly coupled to the top side of the flow cell body; a left side port opening into the chamber adjacent a top of the lower funnel portion of the chamber, the left side port opening for fluid flow into or out of the chamber; a right side port opening into the chamber adjacent the top of the lower funnel portion of the chamber, the right side port opening for fluid flow out of or into the chamber; a bottom side opening into the chamber to allow a fluid stream to exit the chamber and the flow cell body; and wherein the lower funnel portion of the chamber forms the fluid stream out of the bottom side opening.
A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometer or cell sorter system includes a fluidics system and a flow cell. The fluidics system is under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes a drop drive assembly, a flow cell body, and a cuvette coupled together. The drop drive assembly includes a sample injection tube (SIT) in communication with the fluidics system to receive sample fluid. The flow cell body receives the sample fluid from the sample injection tube and sheath fluid. The flow cell body has a charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; and a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein.
A flow cell body for a flow cytometer or a cell sorter is provided. The flow cell body comprises the following: a three-dimensional opaque (e.g., black) polymer body having top, bottom, left, right, front, and back sides. The opaque polymer body includes the following: a top side opening into a chamber to receive a drop drive assembly including a sample injection tube (SIT), wherein the chamber has an upper circular cylindrical portion and a lower funnel portion; two or more opposing top side openings receiving threaded inserts to engage two or more threaded bolts to hold a hub of the drop drive assembly coupled to the top side of the flow cell body; a left side port opening into the chamber adjacent a top of the lower funnel portion of the chamber, the left side port opening for fluid flow into or out of the chamber; a right side port opening into the chamber adjacent the top of the lower funnel portion of the chamber, the right side port opening for fluid flow out of or into the chamber; a bottom side opening into the chamber to allow a fluid stream to exit the chamber and the flow cell body; and wherein the lower funnel portion of the chamber forms the fluid stream out of the bottom side opening.
A subsystem for a flow cytometer or cell sorter system is provided. The subsystem comprises the following: a carriage assembly including a mount having a through hole, wherein the mount is formed to register a nozzle assembly having a nozzle insert with a center nozzle orifice, wherein a gasket is coupled around a perimeter of the nozzle insert, and wherein the mount registers the nozzle assembly such that the center nozzle orifice and the through hole of the mount are concentric along a center axis; the carriage assembly further including a carriage plate statically coupled to the mount such that the carriage plate enables the mount to have vertical movement along the center axis, wherein the mount presses the gasket on the nozzle assembly against a lower side of a cuvette, and wherein the cuvette is formed to have a lower side facing the mount and an upper side coupled to a base of a flow cell body.
A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometer or cell sorter system includes a fluidics system and a flow cell. The fluidics system is under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes a drop drive assembly, a flow cell body, and a cuvette coupled together. The drop drive assembly includes a sample injection tube (SIT) in communication with the fluidics system to receive sample fluid. The flow cell body receives the sample fluid from the sample injection tube and sheath fluid. The flow cell body has a charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; and a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein.
A flow cell body for a flow cytometer or a cell sorter is provided. The flow cell body comprises the following: a three-dimensional opaque (e.g., black) polymer body having top, bottom, left, right, front, and back sides. The opaque polymer body includes the following: a top side opening into a chamber to receive a drop drive assembly including a sample injection tube (SIT), wherein the chamber has an upper circular cylindrical portion and a lower funnel portion; two or more opposing top side openings receiving threaded inserts to engage two or more threaded bolts to hold a hub of the drop drive assembly coupled to the top side of the flow cell body; a left side port opening into the chamber adjacent a top of the lower funnel portion of the chamber, the left side port opening for fluid flow into or out of the chamber; a right side port opening into the chamber adjacent the top of the lower funnel portion of the chamber, the right side port opening for fluid flow out of or into the chamber; a bottom side opening into the chamber to allow a fluid stream to exit the chamber and the flow cell body; and wherein the lower funnel portion of the chamber forms the fluid stream out of the bottom side opening.
A subsystem for a flow cytometer or cell sorter system is provided. The subsystem comprises the following: a carriage assembly including a mount having a through hole, wherein the mount is formed to register a nozzle assembly having a nozzle insert with a center nozzle orifice, wherein a gasket is coupled around a perimeter of the nozzle insert, and wherein the mount registers the nozzle assembly such that the center nozzle orifice and the through hole of the mount are concentric along a center axis; the carriage assembly further including a carriage plate statically coupled to the mount such that the carriage plate enables the mount to have vertical movement along the center axis, wherein the mount presses the gasket on the nozzle assembly against a lower side of a cuvette, and wherein the cuvette is formed to have a lower side facing the mount and an upper side coupled to a base of a flow cell body.
A flow cytometer or cell sorter system is provided. The system comprises the following: a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid, the flow cell including a flow cell body coupled around the drop drive assembly to receive the sample fluid from the sample injection tube, the flow cell body coupled in communication with the fluidics system to receive the sheath fluid, the flow cell body having charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein; and a nozzle assembly selectively engaged with the cuvette, the nozzle assembly having a nozzle and an O-ring around the nozzle selectively pressed against a face of the cuvette around the channel, the nozzle receiving the sample stream from the cuvette and forming sample drops out of the nozzle assembly.
A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometer or cell sorter system includes a fluidics system and a flow cell. The fluidics system is under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes a drop drive assembly, a flow cell body, and a cuvette coupled together. The drop drive assembly includes a sample injection tube (SIT) in communication with the fluidics system to receive sample fluid. The flow cell body receives the sample fluid from the sample injection tube and sheath fluid. The flow cell body has a charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; and a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein.
A flow cell body for a flow cytometer or a cell sorter is provided. The flow cell body comprises the following: a three-dimensional opaque (e.g., black) polymer body having top, bottom, left, right, front, and back sides. The opaque polymer body includes the following: a top side opening into a chamber to receive a drop drive assembly including a sample injection tube (SIT), wherein the chamber has an upper circular cylindrical portion and a lower funnel portion; two or more opposing top side openings receiving threaded inserts to engage two or more threaded bolts to hold a hub of the drop drive assembly coupled to the top side of the flow cell body; a left side port opening into the chamber adjacent a top of the lower funnel portion of the chamber, the left side port opening for fluid flow into or out of the chamber; a right side port opening into the chamber adjacent the top of the lower funnel portion of the chamber, the right side port opening for fluid flow out of or into the chamber; a bottom side opening into the chamber to allow a fluid stream to exit the chamber and the flow cell body; and wherein the lower funnel portion of the chamber forms the fluid stream out of the bottom side opening.
A subsystem for a flow cytometer or cell sorter system is provided. The subsystem comprises the following: a carriage assembly including a mount having a through hole, wherein the mount is formed to register a nozzle assembly having a nozzle insert with a center nozzle orifice, wherein a gasket is coupled around a perimeter of the nozzle insert, and wherein the mount registers the nozzle assembly such that the center nozzle orifice and the through hole of the mount are concentric along a center axis; the carriage assembly further including a carriage plate statically coupled to the mount such that the carriage plate enables the mount to have vertical movement along the center axis, wherein the mount presses the gasket on the nozzle assembly against a lower side of a cuvette, and wherein the cuvette is formed to have a lower side facing the mount and an upper side coupled to a base of a flow cell body.
A flow cytometer or cell sorter system is provided. The system comprises the following: a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid, the flow cell including a flow cell body coupled around the drop drive assembly to receive the sample fluid from the sample injection tube, the flow cell body coupled in communication with the fluidics system to receive the sheath fluid, the flow cell body having charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein; and a nozzle assembly selectively engaged with the cuvette, the nozzle assembly having a nozzle and an O-ring around the nozzle selectively pressed against a face of the cuvette around the channel, the nozzle receiving the sample stream from the cuvette and forming sample drops out of the nozzle assembly.
A nozzle assembly for a cell sorter system is provided. The nozzle assembly comprises the following: a nozzle handle having a body with a gripping end and a nozzle end, the body having a through hole between top and bottom surfaces near the nozzle end with a partial gland in the top surface extending around the through hole, the partial gland having a slot extending out from the through hole to the nozzle end of the nozzle handle; a nozzle insert positioned in a portion of the through hole of the body of the nozzle handle, the nozzle insert having a circular body with a center nozzle orifice concentric with the through hole to flow drops of a sample fluid, and a beveled ring in a top surface extending out from the circular body; a gasket positioned in the partial gland against the beveled ring of the nozzle insert with a portion extending above the top surface of the nozzle insert and the top surface of the nozzle handle, the gasket to provide a seal around the center nozzle orifice; and wherein the slot extending out from the partial gland to the nozzle end facilitates removal of the gasket.
A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometer or cell sorter system includes a fluidics system and a flow cell. The fluidics system is under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes a drop drive assembly, a flow cell body, and a cuvette coupled together. The drop drive assembly includes a sample injection tube (SIT) in communication with the fluidics system to receive sample fluid. The flow cell body receives the sample fluid from the sample injection tube and sheath fluid. The flow cell body has a charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; and a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein.
A flow cell body for a flow cytometer or a cell sorter is provided. The flow cell body comprises the following: a three-dimensional opaque (e.g., black) polymer body having top, bottom, left, right, front, and back sides. The opaque polymer body includes the following: a top side opening into a chamber to receive a drop drive assembly including a sample injection tube (SIT), wherein the chamber has an upper circular cylindrical portion and a lower funnel portion; two or more opposing top side openings receiving threaded inserts to engage two or more threaded bolts to hold a hub of the drop drive assembly coupled to the top side of the flow cell body; a left side port opening into the chamber adjacent a top of the lower funnel portion of the chamber, the left side port opening for fluid flow into or out of the chamber; a right side port opening into the chamber adjacent the top of the lower funnel portion of the chamber, the right side port opening for fluid flow out of or into the chamber; a bottom side opening into the chamber to allow a fluid stream to exit the chamber and the flow cell body; and wherein the lower funnel portion of the chamber forms the fluid stream out of the bottom side opening.
A subsystem for a flow cytometer or cell sorter system is provided. The subsystem comprises the following: a carriage assembly including a mount having a through hole, wherein the mount is formed to register a nozzle assembly having a nozzle insert with a center nozzle orifice, wherein a gasket is coupled around a perimeter of the nozzle insert, and wherein the mount registers the nozzle assembly such that the center nozzle orifice and the through hole of the mount are concentric along a center axis; the carriage assembly further including a carriage plate statically coupled to the mount such that the carriage plate enables the mount to have vertical movement along the center axis, wherein the mount presses the gasket on the nozzle assembly against a lower side of a cuvette, and wherein the cuvette is formed to have a lower side facing the mount and an upper side coupled to a base of a flow cell body.
A flow cytometer or cell sorter system is provided. The system comprises the following: a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid, the flow cell including a flow cell body coupled around the drop drive assembly to receive the sample fluid from the sample injection tube, the flow cell body coupled in communication with the fluidics system to receive the sheath fluid, the flow cell body having charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein; and a nozzle assembly selectively engaged with the cuvette, the nozzle assembly having a nozzle and an O-ring around the nozzle selectively pressed against a face of the cuvette around the channel, the nozzle receiving the sample stream from the cuvette and forming sample drops out of the nozzle assembly.
A nozzle assembly for a cell sorter system is provided. The nozzle assembly comprises the following: a nozzle handle having a body with a gripping end and a nozzle end, the body having a through hole between top and bottom surfaces near the nozzle end with a partial gland in the top surface extending around the through hole, the partial gland having a slot extending out from the through hole to the nozzle end of the nozzle handle; a nozzle insert positioned in a portion of the through hole of the body of the nozzle handle, the nozzle insert having a circular body with a center nozzle orifice concentric with the through hole to flow drops of a sample fluid, and a beveled ring in a top surface extending out from the circular body; a gasket positioned in the partial gland against the beveled ring of the nozzle insert with a portion extending above the top surface of the nozzle insert and the top surface of the nozzle handle, the gasket to provide a seal around the center nozzle orifice; and wherein the slot extending out from the partial gland to the nozzle end facilitates removal of the gasket.
A flow cytometer or cell sorter system is provided. The system comprises the following: a fluidics system under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; and a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes the following: a flow cell body coupled in communication with the fluidics system to receive the sheath fluid, the flow cell body having charging port to charge the droplets, the flow cell body having a chamber with a circular cylindrical portion and a funnel portion, the funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of a bottom side opening; a drop drive assembly coupled to the flow cell body, the drop drive assembly including a glass sample injection tube (SIT) inserted into the chamber of the flow cell body and having a first end located in the funnel portion of the chamber, the glass sample injection tube having a second end coupled in communication with the fluidics system to receive the sample fluid and inject the sample fluid into the funnel portion of the chamber; and a cuvette coupled to a base of the flow cell body, the cuvette having a flow channel adjacent the bottom side opening of the flow cell body, the cuvette to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the bottom side opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the flow channel by a plurality of different lasers to determine a plurality of different types of cells or particles in the sample fluid.
A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometer or cell sorter system includes a fluidics system and a flow cell. The fluidics system is under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes a drop drive assembly, a flow cell body, and a cuvette coupled together. The drop drive assembly includes a sample injection tube (SIT) in communication with the fluidics system to receive sample fluid. The flow cell body receives the sample fluid from the sample injection tube and sheath fluid. The flow cell body has a charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; and a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein.
A flow cell body for a flow cytometer or a cell sorter is provided. The flow cell body comprises the following: a three-dimensional opaque (e.g., black) polymer body having top, bottom, left, right, front, and back sides. The opaque polymer body includes the following: a top side opening into a chamber to receive a drop drive assembly including a sample injection tube (SIT), wherein the chamber has an upper circular cylindrical portion and a lower funnel portion; two or more opposing top side openings receiving threaded inserts to engage two or more threaded bolts to hold a hub of the drop drive assembly coupled to the top side of the flow cell body; a left side port opening into the chamber adjacent a top of the lower funnel portion of the chamber, the left side port opening for fluid flow into or out of the chamber; a right side port opening into the chamber adjacent the top of the lower funnel portion of the chamber, the right side port opening for fluid flow out of or into the chamber; a bottom side opening into the chamber to allow a fluid stream to exit the chamber and the flow cell body; and wherein the lower funnel portion of the chamber forms the fluid stream out of the bottom side opening.
A subsystem for a flow cytometer or cell sorter system is provided. The subsystem comprises the following: a carriage assembly including a mount having a through hole, wherein the mount is formed to register a nozzle assembly having a nozzle insert with a center nozzle orifice, wherein a gasket is coupled around a perimeter of the nozzle insert, and wherein the mount registers the nozzle assembly such that the center nozzle orifice and the through hole of the mount are concentric along a center axis; the carriage assembly further including a carriage plate statically coupled to the mount such that the carriage plate enables the mount to have vertical movement along the center axis, wherein the mount presses the gasket on the nozzle assembly against a lower side of a cuvette, and wherein the cuvette is formed to have a lower side facing the mount and an upper side coupled to a base of a flow cell body.
A flow cytometer or cell sorter system is provided. The system comprises the following: a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid, the flow cell including a flow cell body coupled around the drop drive assembly to receive the sample fluid from the sample injection tube, the flow cell body coupled in communication with the fluidics system to receive the sheath fluid, the flow cell body having charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein; and a nozzle assembly selectively engaged with the cuvette, the nozzle assembly having a nozzle and an O-ring around the nozzle selectively pressed against a face of the cuvette around the channel, the nozzle receiving the sample stream from the cuvette and forming sample drops out of the nozzle assembly.
A nozzle assembly for a cell sorter system is provided. The nozzle assembly comprises the following: a nozzle handle having a body with a gripping end and a nozzle end, the body having a through hole between top and bottom surfaces near the nozzle end with a partial gland in the top surface extending around the through hole, the partial gland having a slot extending out from the through hole to the nozzle end of the nozzle handle; a nozzle insert positioned in a portion of the through hole of the body of the nozzle handle, the nozzle insert having a circular body with a center nozzle orifice concentric with the through hole to flow drops of a sample fluid, and a beveled ring in a top surface extending out from the circular body; a gasket positioned in the partial gland against the beveled ring of the nozzle insert with a portion extending above the top surface of the nozzle insert and the top surface of the nozzle handle, the gasket to provide a seal around the center nozzle orifice; and wherein the slot extending out from the partial gland to the nozzle end facilitates removal of the gasket.
A flow cytometer or cell sorter system is provided. The system comprises the following: a fluidics system under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; and a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes the following: a flow cell body coupled in communication with the fluidics system to receive the sheath fluid, the flow cell body having charging port to charge the droplets, the flow cell body having a chamber with a circular cylindrical portion and a funnel portion, the funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of a bottom side opening; a drop drive assembly coupled to the flow cell body, the drop drive assembly including a glass sample injection tube (SIT) inserted into the chamber of the flow cell body and having a first end located in the funnel portion of the chamber, the glass sample injection tube having a second end coupled in communication with the fluidics system to receive the sample fluid and inject the sample fluid into the funnel portion of the chamber; and a cuvette coupled to a base of the flow cell body, the cuvette having a flow channel adjacent the bottom side opening of the flow cell body, the cuvette to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the bottom side opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the flow channel by a plurality of different lasers to determine a plurality of different types of cells or particles in the sample fluid.
A drop drive assembly for a flow cytometer or cell sorter system is provided. The drop drive assembly comprises the following: an outer metal (piezo) hub having a center opening extending from top to bottom through an extended hollow circular plug; an insulating spacer extended over the extended hollow circular plug up to a flange in a base of the outer metal hub; a hollow piezoelectric cylindrical transducer having a first end coupled over the extended hollow circular plug up to insulating spacer, an inner terminal of the hollow piezoelectric cylindrical transducer coupled to the extended hollow circular plug to form an electrical connection to the outer metal hub; an insulated cylindrical sealing base having an extended hollow circular plug coupled into a second end of the hollow piezoelectric cylindrical transducer, the insulated cylindrical sealing base having a gland ring around a through hole opposite the extended hollow circular plug, the insulated cylindrical sealing base having a groove around an outer cylindrical surface; a hollow cylindrical glass sample injection tube (SIT) having a first end inserted into and through the center opening in the outer metal (piezo) hub, the hollow piezoelectric cylindrical transducer, and the through hole in the insulated cylindrical sealing base; a first sealing O-ring mounted in the groove around in the outer cylindrical surface of the insulated cylindrical sealing base, the first sealing O-ring to engage a cylindrical wall of a cylindrical chamber to seal fluids away from the hollow piezoelectric cylindrical transducer; and a second sealing O-ring mounted in the gland ring in the insulated cylindrical sealing base around the hollow cylindrical glass sample injection tube (SIT) to seal fluids away from the hollow piezoelectric cylindrical transducer.
A flow cytometry or cell sorting system is disclosed. Under pressure, a fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a degasser. A flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives the drops of sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
G01N 21/49 - Scattering, i.e. diffuse reflection within a body or fluid
G01N 21/63 - Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
42.
MAGNETICALLY COUPLED COLLECTING SYSTEM FOR FLOW CYTOMETRY AND CELL SORTER SYSTEMS
A collecting system with a magnetically coupled sample mover is provided for flow cytometry and cell sorter systems. The collecting system uses magnets in a driver carriage to control the position of other magnets in a follower carriage. The driver carriage can thereby control the position of the follower carriage without physically touching the follower carriage.
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
G01D 5/347 - Mechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using optical means, i.e. using infrared, visible or ultraviolet light with attenuation or whole or partial obturation of beams of light the beams of light being detected by photocells using displacement encoding scales
G01D 5/14 - Mechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage
G01D 5/24 - Mechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying capacitance
43.
MAGNETICALLY COUPLED COLLECTING SYSTEM FOR FLOW CYTOMETRY AND CELL SORTER SYSTEMS
A collecting system with a magnetically coupled sample mover is provided for flow cytometry and cell sorter systems. The collecting system uses magnets in a driver carriage to control the position of other magnets in a follower carriage. The driver carriage can thereby control the position of the follower carriage without physically touching the follower carriage.
A compact sorting flow cytometer system includes a fluidics system having a flow cell and a deflection chamber in communication with the flow cell and a droplet deposition unit (DDU) system in communication with the deflection chamber. The defection chamber receives drops in a stream of a sample biological fluid with one or more biological cells or particles and selectively deflect the drops in the stream. The DDU system is in communication with the deflection chamber to receive the selectively deflected drops into one or more containers. The DDU system includes a case forming a portion of a containment chamber. When closed, one or more doors seal and close off the containment chamber from an external environment. Air can be evacuated from the containment chamber by one or more fans in one or more tunnels. Air can be conditioned by a thermoelectric cooling array between the containment chamber and an air conditioning chamber that is moved by one or more fans.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
A compact sorting flow cytometer system is disclosed. The system includes a fluidics system having a flow cell and a deflection chamber in communication with the flow cell to receive drops in a stream of a sample biological fluid with one or more biological cells or particles and selectively deflect the drops in the stream of the sample biological fluid with the one or more biological cells or particles; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers. The DDU system includes a case or a housing with an open face surround by edges of the case, the case forming a portion of a containment chamber, the case having a top side opening aligned with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid into one or more containers in the containment chamber, a seal mounted around edges of the case, one or more hinges coupled to a bottom portion of the case, and a door coupled to the one or more hinges to pivot the door about the one or more hinges, the door when closed to press against the seal and close off the containment chamber from an external environment. A method for evacuation of air in a containment chamber of a flow cytometer is disclosed. The method includes turning off a return fan in a first tunnel between an air conditioning chamber and a containment chamber; turning on an evacuation fan in a second tunnel between the air conditioning chamber and the containment chamber, the evacuation fan pulling air out of the containment chamber into the air conditioning chamber, opening a valve in an evacuation vent, the evacuation fan pushing air out of the air conditioning chamber through the evacuation vent into the environment; and continuously running the evacuation fan for a predetermined period of time to evacuate air out of the containment chamber.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Biochemical reagents commonly known as probes, for detecting and analyzing molecules in protein or nucleotide arrays; Biochemical reagents used for non-medical purposes; Biochemicals for in vitro and in vivo scientific use; Buffer solutions for use in analytical chemistry; Reagents for scientific and research use; Diagnostic reagents for scientific or research use
47.
Methods and apparatus for self-triggering flow cytometers
A flow-cytometer has an excitation light source generating an excitation light that excites one or more bio-cells in a bio-sample carried by a flow path to luminesce. The flow-cytometer includes a spectrum dispersive element that disperses the luminescent light generated by the bio-sample into a photo-detector array. The flow-cytometer further includes a digital signal processor (DSP) that receives signals from the photo-detector array and generates a self-triggering signal based on the luminescent light generated by the bio-cells in bio-sample. The self-triggering signal triggers data capture in the DSP to improve synchronization with the data generated from signals received from the photo-detector array.
A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.
In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
50.
METHODS AND APPARATUS FOR FULL SPECTRUM FLOW CYTOMETER
In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Laboratory instruments and equipment, namely, flow
cytometers and cell sorters for scientific, laboratory, and
general research uses. Medical instruments, namely, flow cytometers and cell
sorters for medical, clinical, medical diagnostic, and
therapeutic uses.
52.
Methods and apparatus for central source pressure-based cytometer fluidics system
A system, method, and apparatus are provided for cytometer fluidics. In one example, a pressure regulation system is provided for a cytometer fluidics system. The pressure regulation system includes a pressure regulator and a transducer. The pressure regulator pressurizes sheath fluid in a fluid path. The transducer senses a measured pressure in the fluid path independently of the sheath tank. The transducer converts the measured pressure into a voltage. The transducer communicates the voltage to the pressure regulator. The pressure regulator translates the voltage to a regulated pressure that substantially matches the measured pressure. The pressure regulator is the only pressure regulation source in the cytometry fluidics system.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
(1) Laboratory instruments and equipment, namely, flow cytometers and cell sorters for scientific, laboratory, and general research uses.
(2) Medical instruments, namely, flow cytometers and cell sorters for medical, clinical, medical diagnostic, and therapeutic uses.
54.
METHODS FOR COMPACT MULTI-COLOR FLOW CYTOMETERS HAVING COMPACT DETECTION MODULES
A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.
In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network. A central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers. Each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure. The monitoring system can detect an advanced failure of components based on the operational parameters being monitored. The monitoring system can also detect an advanced need for repair and maintenance based on the operational parameters being monitored.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Providing a website featuring educational information in the field of flow cytometry; Providing a website featuring educational information in the field of biological science
In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Laboratory instruments and equipment, namely, flow cytometers and cell sorters for scientific, laboratory, and general research uses Medical instruments, namely, flow cytometers and cell sorters for medical, clinical, medical diagnostic, and therapeutic uses
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemical solutions and preparations (term considered too
vague by the International Bureau - Rule 13 (2) (b) of the
Regulations); reactants (term considered too vague by the
International Bureau - Rule 13 (2) (b) of the Regulations);
pre-mixed reactants (term considered too vague by the
International Bureau - Rule 13 (2) (b) of the Regulations);
reagents (term considered too vague by the International
Bureau - Rule 13 (2) (b) of the Regulations); pre-mixed
reagents (term considered too vague by the International
Bureau - Rule 13 (2) (b) of the Regulations); reagents for
scientific or medical research use; reagents with markers
for labelling or marking biological cells and other
molecules; reagents with conjugated antibodies for labelling
or marking cells in biological samples; kits comprised of
such chemical solutions and preparations, reactants, or
reagents (term considered too vague by the International
Bureau - Rule 13 (2) (b) of the Regulations).
61.
Linear resistance stepper flow control valve for sheath fluid of flow cytometry systems
A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell. A stepper flow control valves are disclosed that apply a physical fluid resistance to a flow of sheath fluid in the flow cytometer. The physical fluid resistance regulates a flow rate of the sheath fluid and thereby regulates a flow rate of sample fluid in the flow cytometer.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Laboratory instruments, apparatus, and equipment, namely, flow cytometers for scientific, laboratory, and general research uses Medical instruments, apparatus, and equipment, namely, flow cytometers for medical, clinical, medical diagnostic, and therapeutic uses
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemical solutions and preparations, namely, reactants, pre-mixed reactants, reagents, and pre-mixed reagents for scientific or medical research use; Reagents for scientific or medical research use; Reagents with markers for labelling or marking biological cells and other molecules for scientific or medical research use; Reagents with conjugated antibodies for labelling or marking cells in biological samples for scientific or medical research use; Kits comprised of chemical solutions and preparations, consisting primarily of reactants for scientific or medical research use; and Kits comprised of chemical solutions and preparations, consisting primarily of reagents for scientific or medical research use
09 - Scientific and electric apparatus and instruments
Goods & Services
Downloadable computer software and software recorded on computer media for clinical and laboratory instruments for analyzing biological fluids
66.
Method to combine brightfield and fluorescent channels for cell image segmentation and morphological analysis using images obtained from imaging flow cytometer (IFC)
A classifier engine provides cell morphology identification and cell classification in computer-automated systems, methods and diagnostic tools. The classifier engine performs multispectral segmentation of thousands of cellular images acquired by a multispectral imaging flow cytometer. As a function of imaging mode, different ones of the images provide different segmentation masks for cells and subcellular parts. Using the segmentation masks, the classifier engine iteratively optimizes model fitting of different cellular parts. The resulting improved image data has increased accuracy of location of cell parts in an image and enables detection of complex cell morphologies in the image. The classifier engine provides automated ranking and selection of most discriminative shape based features for classifying cell types.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Unprocessed polymer material in the nature of beads, micro-beads, test beads, quality control beads, counting beads, optimization beads, compensation beads, capture beads, and compensation capture beads for use in calibrating and assuring quality in the operation of sorting flow cytometers or cell sorters, but not spectrometers, for medical, scientific, laboratory, or research use; organically dyed polystyrene beads for use in calibrating and assuring quality in the operation of sorting flow cytometers or cell sorters, but not spectrometers, for medical, scientific, laboratory, or research use; and calibration micro-spheres and calibration particles in the nature of organically dyed polystyrene beads in a water solution for use in calibrating or assuring quality in the operation of sorting flow cytometers or cell sorters, but not spectrometers, for medical, scientific, laboratory, or research use
A crystal for flow cytometry with dual laser beams is disclosed. The crystal is a birefringent crystal comprising a material composition including a quartz mineral having a face side including a face angle of ninety degrees plus or minus one tenth of a degree; a wedge side that is substantially perpendicular to the face side, wherein the wedge side includes a wedge angle of two degrees plus or minus one tenth of a degree; and a major side that is substantially perpendicular to the face side and the wedge side. The major side includes a thickness of one and one-half millimeter plus or minus one tenth of a millimeter. A polarized light beam entering the birefringent crystal at an incident angle is separated into an ordinary light beam and an extraordinary light beam.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
(1) Reagents, namely antibody reagents for scientific purposes, chemical reagents for use in medical biotechnology scientific research, cell culture reagents for laboratory use, cell culture reagents for scientific or research purposes; reagents for scientific or medical research use, namely in vitro diagnostic reagents for scientific purposes, diagnostic reagents for medical-scientific research use; diagnostic reagents with markers for labelling and marking biological cells and other molecules for medical-scientific research use; antibody reagents with conjugated antibodies for labelling and marking cells in biological samples for scientific purposes; kits comprised of such chemical solutions and preparations, reactants, or reagents, namely kits comprising chemical reagents for identifying cell subsets in human blood, such kits being used for scientific research purposes.
A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Diagnostic reagents, contrast dyes, dyes to label or stain biological cells, used singly or in combination with other materials and in kits, for medical use; Diagnostic preparations for medical purposes, namely, diagnostic agents and diagnostic reagents; Contrast dyes, stains, namely, fluorescent dyes and chemicals for medical use; Dyes, stains, namely, fluorescent dyes and chemicals for labeling or staining biological cells for medical purposes; and Fluorescent brightening agents, namely fluorescent dyes, stains, and chemicals for labeling or staining biological cells for medical purposes
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Diagnostic reagents, contrast dyes, dyes to label or stain biological cells, used singly or in combination with other materials and in kits, for scientific, laboratory, or research use; Diagnostic preparations for scientific, laboratory, and research purposes, namely, diagnostic agents, and diagnostic reagents; Contrast dyes, stains, namely, fluorescent dyes and chemicals for scientific, laboratory, or research use; Dyes, stains, namely, fluorescent dyes and chemicals for labeling or staining biological cells for scientific, laboratory, or research purposes; and Fluorescent brightening agents, namely, fluorescent dyes, stains, and chemicals for labeling or staining biological cells for scientific, laboratory, or research purposes
09 - Scientific and electric apparatus and instruments
16 - Paper, cardboard and goods made from these materials
42 - Scientific, technological and industrial services, research and design
Goods & Services
Downloadable software for use by professionals in the field of science and medicine; Digital media, namely, a downloadable document file, and a downloadable poster for use by professionals in the field of science and medicine; and Electronic downloadable publications, namely, a downloadable document file, and a downloadable poster for use by professionals in the field of science and medicine Posters; and Educational publications, namely, posters in the field of biological science and flow cytometry Providing a website featuring information in the field of flow cytometry; and Software as a service (SAAS) services featuring software for selecting dyes, fluorochromes, and fluorescent proteins in the field of flow cytometry
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Chemical solutions and preparations, namely, reactants, pre-mixed reactants, reagents, and pre-mixed reagents for scientific or medical research use; Reagents for scientific or medical research use; Reagents with markers for labelling or marking biological cells and other molecules for scientific or medical research use; Reagents with conjugated antibodies for labelling or marking cells in biological samples for scientific or medical research use; Diagnostic reagents for clinical or medical laboratory use; Kits comprised of chemical solutions and preparations, consisting primarily of reactants for scientific or medical research use; and Kits comprised of chemical solutions and preparations, consisting primarily of reagents for scientific or medical research use Clinical medical reagents
75.
Smart flow cytometers with self monitoring and self validation
In some embodiments, a smart flow cytometer includes a monitoring system to monitor differing operational parameters of the smart flow cytometer to detect an advanced failure of components and an advanced need for maintenance. In others, a smart flow cytometer includes a quality control system including a reservoir of quality control beads to periodically run a validation test with the quality control beads in order to determine the ability of the flow cytometer to generate quality data output from a plurality of light detectors. In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network; a central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers; wherein each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure.
In some embodiments, a smart flow cytometer includes a monitoring system to monitor differing operational parameters of the smart flow cytometer to detect an advanced failure of components and an advanced need for maintenance. In others, a smart flow cytometer includes a quality control system including a reservoir of quality control beads to periodically run a validation test with the quality control beads in order to determine the ability of the flow cytometer to generate quality data output from a plurality of light detectors. In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network; a central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers; wherein each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure.
n one embodiment, a method of performing fast compensation in a flow cytometry experiment is provided. The method includes the following: generating an initial spillover matrix by using a plurality of single stained compensation controls; running a sample through the flow cytometer; generating a measured sample event vector by measuring fluorescence of a plurality of cells passing through the flow cytometer; generating a compensated sample event vector by using the initial spillover matrix and the measured sample event vector; generating an adjusted spillover matrix by finely adjusting the initial spill-over matrix; and calculating a re-compensated event vector by using the adjusted spillover matrix and the measured sample event vector.
In one embodiment, a method of performing fast compensation in a flow cytometry experiment is provided. The method includes the following: generating an initial spillover matrix by using a plurality of single stained compensation controls; running a sample through the flow cytometer; generating a measured sample event vector by measuring fluorescence of a plurality of cells passing through the flow cytometer; generating a compensated sample event vector by using the initial spillover matrix and the measured sample event vector; generating an adjusted spillover matrix by finely adjusting the initial spill-over matrix; and calculating a re-compensated event vector by using the adjusted spillover matrix and the measured sample event vector.
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
79.
Method to combine brightfield and fluorescent channels for cell image segmentation and morphological analysis using images obtained from imaging flow cytometer (IFC)
A classifier engine provides cell morphology identification and cell classification in computer-automated systems, methods and diagnostic tools. The classifier engine performs multispectral segmentation of thousands of cellular images acquired by a multispectral imaging flow cytometer. As a function of imaging mode, different ones of the images provide different segmentation masks for cells and subcellular parts. Using the segmentation masks, the classifier engine iteratively optimizes model fitting of different cellular parts. The resulting improved image data has increased accuracy of location of cell parts in an image and enables detection of complex cell morphologies in the image. The classifier engine provides automated ranking and selection of most discriminative shape based features for classifying cell types.
A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell. A stepper flow control valves are disclosed that apply a physical fluid resistance to a flow of sheath fluid in the flow cytometer. The physical fluid resistance regulates a flow rate of the sheath fluid and thereby regulates a flow rate of sample fluid in the flow cytometer.
A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.
A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell. A stepper flow control valves are disclosed that apply a physical fluid resistance to a flow of sheath fluid in the flow cytometer. The physical fluid resistance regulates a flow rate of the sheath fluid and thereby regulates a flow rate of sample fluid in the flow cytometer.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Unprocessed polymer resins in bead form for calibrating and
assuring quality in the operation of flow cytometers;
unprocessed polystyrene resins for scientific or medical
research in bead form.
In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.
G06F 19/00 - Digital computing or data processing equipment or methods, specially adapted for specific applications (specially adapted for specific functions G06F 17/00;data processing systems or methods specially adapted for administrative, commercial, financial, managerial, supervisory or forecasting purposes G06Q;healthcare informatics G16H)
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
G01N 21/00 - Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
G01N 21/77 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
A system, method, and apparatus are provided for cytometry with dual laser beams. In one example, the method includes directing an incident light beam from a source to enter an optical waveplate; polarizing the incident light beam into a polarized light beam in response to the incident light beam entering through the optical waveplate; directing the polarized light beam to enter a birefringent crystal; separating the polarized light beam into an ordinary light beam and an extraordinary light beam in response to the polarized light beam entering the birefringent crystal; directing the ordinary light beam and the extraordinary light beam to enter a lens; focusing the ordinary light beam and the extraordinary light beam into dual light beams separated by a beam displacement; and coupling the dual light beams to form a sample region having substantially uniform light intensity to analyze moving particles in the particle analyzer.
A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.
A system, method, and apparatus are provided for cytometry with dual laser beams. In one example, the method includes directing an incident light beam from a source to enter an optical waveplate; polarizing the incident light beam into a polarized light beam in response to the incident light beam entering through the optical waveplate; directing the polarized light beam to enter a birefringent crystal; separating the polarized light beam into an ordinary light beam and an extraordinary light beam in response to the polarized light beam entering the birefringent crystal; directing the ordinary light beam and the extraordinary light beam to enter a lens; focusing the ordinary light beam and the extraordinary light beam into dual light beams separated by a beam displacement; and coupling the dual light beams to form a sample region having substantially uniform light intensity to analyze moving particles in the particle analyzer.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
(1) Beads used in the initialization, calibration and testing of flow cytometers; quality control beads, compensation particles, counting beads, standardization beads, capture beads, all for use with flow cytometers.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Cell analysis instrument for laboratory purposes in cellular
research, immunology, molecular biology, cancer research. Cell analysis instrument for medical purposes in cellular
research, immunology, molecular biology, cancer research.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
(1) Cell analysis tool for laboratory purposes in cellular research, immunology, molecular biology, cancer research, namely, a flow cytometer.
(2) Cell analysis tool for medical purposes in cellular research, immunology, molecular biology, cancer research, namely, a flow cytometer.
01 - Chemical and biological materials for industrial, scientific and agricultural use
17 - Rubber and plastic; packing and insulating materials
Goods & Services
Unprocessed polymer material in the nature of beads, micro-beads, test beads, quality control beads, counting beads, optimization beads, compensation beads, capture beads, and compensation capture beads for use in calibrating and assuring quality in the operation of flow cytometers and scientific or research use; unprocessed polystyrene resin in the nature of beads for use in calibrating and assuring quality in the operation of flow cytometers and medical or scientific research; calibrating and quality control particles in the nature of microspheres and beads of unprocessed artificial resin for use in calibrating and assuring quality in the operation of flow cytometers and medical or scientific research Semi-processed polystyren resin in the nature of beads for use in calibrating and assuring quality in the operation of flow cytometers
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Laboratory instruments and equipment, namely, flow cytometers for scientific and general research uses Medical instruments, namely, flow cytometers for analyzing biological fluids for medical diagnostic uses; flow cytometers for medical diagnostic uses
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Cell analysis tool for laboratory purposes in cellular research, immunology, molecular biology, cancer research, namely, a flow cytometer Cell analysis tool for medical purposes in cellular research, immunology, molecular biology, cancer research, namely, a flow cytometer
A disposable rapid cell sorter comprises a microfluidic chip with electrodes and sorts biological cells of interest though a magnetic field and an electric field based on biological cell functional antibody bonded magnetic beads and luminescent labeling.
A flow-cytometer has an excitation light source generating an excitation light that excites one or more bio-cells in a bio-sample carried by a flow path to luminesce. The flow-cytometer includes a spectrum dispersive element that disperses the luminescent light generated by the bio-sample into a photo-detector array. The flow-cytometer further includes a digital signal processor (DSP) that receives signals from the photo-detector array and generates a self-triggering signal based on the luminescent light generated by the bio-cells in bio-sample. The self-triggering signal triggers data capture in the DSP to improve synchronization with the data generated from signals received from the photo-detector array.
