The present invention relates to the technical field of genetic engineering, and particularly, to a glutamine synthetase mutant with glufosinate resistance. According to the present invention, mutations are induced at positions 55 and 64 of the amino acid sequence of a glutamine synthetase wild-type, and the glutamine synthetase mutants with enhanced glufosinate resistance are selected. Plants transformed with the mutant shall have glufosinate resistance suitable for commercial applications while retaining the normal enzymatic activity of glutamine synthetase, which meets the requirement of the normal growth and development of the plants.
A glutamine synthetase mutant having glufosinate-ammonium resistance and an application thereof, relating to the field of gene engineering. The glutamine synthetase mutant having glufosinate-ammonium resistance is obtained by mutating an nth site of wild-type glutamine synthetase, the mutated site is A, C, D, E, F, G, H, I, L, M, N, Q, R, S, T, V, and W, or is deleted, and such mutation imparts glufosinate-ammonium resistance to the glutamine synthetase. Plants transformed with the glutamine synthetase mutant have glufosinate-ammonium resistance, and can also normally grow and develop. Therefore, the glutamine synthetase mutant can be used to breed new varieties of glufosinate-ammonium resistant plants, and glufosinate-ammonium resistant recombinant bacteria and recombinant cells.
The present disclosure provides a glutamine synthetase mutant and an application thereof in cultivating a glufosinate-resistant plant variety. The glutamine synthetase mutant is obtained by mutating an n-th position of a wild-type glutamine synthetase, and the site corresponds to the 62-th position of an amino acid sequence shown in SEQ ID NO:1.
Provided are a glutamine synthetase mutant and an application thereof in breeding of glufosinate-ammonium-resistant plant varieties. The glutamine synthetase mutant is obtained by mutation at position n of a wild-type glutamine synthetase, the position is A, G, M, N, Q, S, T, V or deleted after the mutation, and the position corresponds to position 69 of an amino acid sequence as represented by SEQ ID NO. 1.
Provided are a glutamine synthetase mutant having glufosinate-ammonium resistance, a nucleic acid molecule and the use, which relate to the technical field of genetic engineering. The glutamine synthetase mutant can be obtained by means of mutation at the nth site of wild glutamine synthetase. After mutation, the nth site is transformed into C, E, F, I, M, N, P, S and Y or is deleted, and the mutation can endow the glutamine synthetase with glufosinate-ammonium resistance suitable for commercial application. The glutamine synthetase mutant has application potential in terms of constructing expression vectors for transforming plants and cultivating glufosinate-ammonium-resistant crops.
Provided are an iron/ascorbate-dependent oxidoreductase (HIR) mutant having resistance to triketone herbicides and an application thereof in plant breeding. The HIR mutant is obtained by mutating wild-type HIR derived from a plant, and can degrade triketone herbicides, so that plants expressing the mutant are resistant to triketone herbicides.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
7.
GLUTAMINE SYNTHETASE MUTANT HAVING GLUFOSINATE AMMONIUM RESISTANCE AND APPLICATION THEREOF AND CULTIVATION METHOD THEREFOR
Glutamine synthetase mutant having glufosinate ammonium resistance, application thereof and a cultivation method therefor. Comparing with the reference sequence, the amino acid sequence of the glutamine synthetase mutant has one or a combination of the following mutations: (1) the amino acid of the glutamine synthetase mutant corresponding to amino acid site 59 of the reference sequence is mutated to X1, wherein X1=A, C, D, E, F, G, H, I, K, P, T, V or Y; (2) the amino acid of the glutamine synthetase mutant corresponding to amino acid site 296 of the reference sequence is mutated to X2, wherein X2=A, D, E, G, I, K, M, P, Q, R, S, T, or V.
Provided are a plant-derived glufosinate-ammonium-resistant glutamine synthase mutant, a nucleic acid molecule, and applications. Compared with wild-type glutamine synthases, the mutant has a mutation at position 68, which turns to D, E, G, H, N, P, Q, V or deletion after the mutation, and such mutation gives a glutamine synthase glufosinate-ammonium resistance. The glutamine synthase mutant can be used for breeding new plant varieties having glufosinate-ammonium resistance.
E. coli EPSPS. The mutation of either of the two sites or the simultaneous mutation of the two sites can confer or improve the resistance of the plant EPSPS mutant to glyphosate. Plants or recombinant bacteria for transforming the plant EPSPS mutant can grow normally in the presence of glyphosate.
The invention provides a plant EPSPS mutant (i.e., 5-enolpyruvylshikimate-3-phosphate synthase mutant), which is derived from a plant and is mutated to have glyphosate resistance. Also provided is an encoding gene, which can encode the plant EPSPS mutant. In addition, a vector containing the encoding gene and a cell containing the vector is provided by the invention. Further, a use of the plant EPSPS mutant is provided.
The invention provides a plant EPSPS mutant (i.e. 5-enolpyruvylshikimate-3-phosphate synthase mutant), which is derived from plant, and has glyphosate resistance after mutation. Also provided is an encoding gene, which can encode the above plant EPSPS mutant; and a vector containing the above encoding gene; and a cell containing the above vector. Further provided are uses of the above plant EPSPS mutant.
A paddy rice EPSPS mutant, and an encoding gene and use thereof, relating to the technical field of genetic engineering. The paddy rice EPSPS mutant has an amino acid sequence shown as SEQ ID NO. 1. The paddy rice EPSPS mutant has glyphosate resistance and can resist glyphosate having a concentration of 100 mM. The paddy rice EPSPS mutant has very wide use prospect in the field of cultivating glyphosate-resistant plants.
Provided are a plant EPSPS mutant containing mutations L195P and S247G and an encoding gene and the use thereof, related to the field of genetic engineering technology. Comparing the plant EPSPS mutant with E. coli EPSPS, the amino acid sequence of the plant EPSPS mutant has the mutation L195P at position 195 corresponding to E. coli EPSPS and/or the mutation S247G at position 247 corresponding to E. coli EPSPS. The mutation of either of the two sites or the simultaneous mutation of the two sites can confer or improve the resistance of the plant EPSPS mutant to glyphosate. Plants or recombinant bacteria for transforming the plant EPSPS mutant can grow normally in the presence of glyphosate. The plant EPSPS mutant can not only be used for the cultivation of transgenic crops, but also be used for cultivating non-transgenic plants resistant to glyphosate, such as rice, tobacco, soybean, corn, wheat, cotton and sorghum.
Provided are a plant EPSPS mutant containing mutations L195P and S247G and an encoding gene and the use thereof, related to the field of genetic engineering technology. Comparing the plant EPSPS mutant with E. coli EPSPS, the amino acid sequence of the plant EPSPS mutant has the mutation L195P at position 195 corresponding to E. coli EPSPS and/or the mutation S247G at position 247 corresponding to E. coli EPSPS. The mutation of either of the two sites or the simultaneous mutation of the two sites can confer or improve the resistance of the plant EPSPS mutant to glyphosate. Plants or recombinant bacteria for transforming the plant EPSPS mutant can grow normally in the presence of glyphosate. The plant EPSPS mutant can not only be used for the cultivation of transgenic crops, but also be used for cultivating non-transgenic plants resistant to glyphosate, such as rice, tobacco, soybean, corn, wheat, cotton and sorghum.
Provided are a glyphosate-resistant gene screening method, an EPSPS mutant gene having glyphosate resistance screened by the method, an EPSPS and C-P Lyase deficient strain and a use thereof.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
19.
A138T MUTATION-CONTAINING PLANT EPSPS MUTANT, AND ENCODING GENE AND APPLICATION THEREOF
Provided are an A138T mutation-containing plant EPSPS mutant, and an encoding gene and an application thereof. The site at position 138, corresponding to escherichia coli EPSPS, of the amino acid sequence of the plant EPSPS mutant has the mutation A>T. The mutation significantly increases the resistance of different EPSPS mutants of various plants to glyphosate, and maintains the biological enzyme catalysis activity. A plant or recombinant strain transformed with the plant EPSPS mutant can grow normally in the presence of glyphosate. The plant EPSPS mutant may be used for genetically modified crop cultivation, and may also be used for cultivating glyphosate-resistant non-genetically modified plants, such as rice, tobacco, soybean, corn, wheat, cotton and sorghum.
Provided are a K85 mutation-containing plant EPSPS mutant, and an encoding gene and an application thereof. The site at position 85, corresponding to escherichia coli EPSPS, of the amino acid sequence of the plant EPSPS mutant has a K85T or K85I mutation. The mutation of the site increases the resistance of different EPSPS mutants of various plants to glyphosate, and maintains the biological enzyme catalysis activity. A plant or recombinant strain transformed with the plant EPSPS mutant can grow normally in the presence of glyphosate. The plant EPSPS mutant may be used for genetically modified crop cultivation.
Provided are an A138T mutation-containing plant EPSPS mutant, and an encoding gene and an application thereof. The site at position 138, corresponding to escherichia coli EPSPS, of the amino acid sequence of the plant EPSPS mutant has the mutation A>T. The mutation significantly increases the resistance of different EPSPS mutants of various plants to glyphosate, and maintains the biological enzyme catalysis activity. A plant or recombinant strain transformed with the plant EPSPS mutant can grow normally in the presence of glyphosate. The plant EPSPS mutant may be used for genetically modified crop cultivation, and may also be used for cultivating glyphosate-resistant non-genetically modified plants, such as rice, tobacco, soybean, corn, wheat, cotton and sorghum.
Provided are a K85 mutation-containing plant EPSPS mutant, and an encoding gene and an application thereof. The site at position 85, corresponding to escherichia coli EPSPS, of the amino acid sequence of the plant EPSPS mutant has a K85T or K85I mutation. The mutation of the site increases the resistance of different EPSPS mutants of various plants to glyphosate, and maintains the biological enzyme catalysis activity. A plant or recombinant strain transformed with the plant EPSPS mutant can grow normally in the presence of glyphosate. The plant EPSPS mutant may be used for genetically modified crop cultivation.
A paddy rice EPSPS mutant, and an encoding gene and use thereof, relating to the technical field of genetic engineering. The paddy rice EPSPS mutant has an amino acid sequence shown as SEQ ID NO.1. The paddy rice EPSPS mutant has glyphosate resistance and can resist glyphosate having a concentration of 100mM. The paddy rice EPSPS mutant has very wide use prospect in the field of cultivating glyphosate-resistant plants.
A paddy rice EPSPS mutant, and an encoding gene and use thereof, relating to the technical field of genetic engineering. The paddy rice EPSPS mutant has an amino acid sequence shown as SEQ ID NO.1. The paddy rice EPSPS mutant has glyphosate resistance and can resist glyphosate having a concentration of 100mM. The paddy rice EPSPS mutant has very wide use prospect in the field of cultivating glyphosate-resistant plants.
Provided are a glyphosate-resistant gene screening method, an EPSPS mutant gene having glyphosate resistance screened by the method, an EPSPS and C-P Lyase deficient strain and a use thereof.
Provided are a glyphosate-resistant gene screening method, an EPSPS mutant gene having glyphosate resistance screened by the method, an EPSPS and C-P Lyase deficient strain and a use thereof.
Provided is a method of screening an glyphosate-tolerant EPSPS gene. In this method, a high-throughput screening on microorganisms is performed by using a medium containing glyphosate, and thus the glyphosate-tolerant EPSPS gene can be screened, isolated and analyzed in a short time. Provided also is use of the resistance gene screened by using the method for screening the glyphosate-tolerant EPSPS gene in cultivation of glyphosate-tolerance plant varieties.
The present invention discloses a method for screening a gene for resistance against an HPPD inhibitor-type herbicide. The method comprises high-throughput screening of a microorganism in a nutrient medium containing an HPPD inhibitor, using 16s rRNA sequencing to identify the screened microorganism, using the identification results to determine the species and genus of the screened microorganism, and cloning an HPPD gene for verification.
