The present disclosure belongs to the field of plant breeding and biotechnology, disclosing a method for inducing apomixis in plants, specifically involving the application of the WUS gene in inducing apomixis. The inventors' research revealed that the MOC3 gene in rice (renamed as OsWUS) can be used to construct apomictic lines. In these lines, the seed-setting rate and the induction rate of clonal seeds exhibited varying degrees of change, with some lines maintaining a normal seed-setting rate and achieving a clonal seed efficiency of up to 22%. Therefore, the present disclosure has significant application value.
The present invention belongs to the fields of plant breeding and biotechnology. Disclosed is a method for inducing apomixis in plants. Specifically, the present invention relates to the use of the WUS gene in the induction of apomixis. It is found in the research of the present inventors that the MOC3 gene (renamed OsWUS) in rice can be used for constructing apomictic lines. In these lines, the seed-setting rate and the induction rate of clonal seeds show different degrees of changes. In particular, some lines maintain a normal seed-setting rate, and achieve a clonal seed efficiency of up to 22%. Therefore, the present invention has relatively good application values.
A plant breeding method includes: utilizing technical means to mutate MiMe key genes, screen lines with heterozygous gene loci, and create heterozygous MiMe material; hybridizing heterozygous MiMe material with other plants, and separating new heterozygous MiMe lines from offspring to achieve strategic purpose of transferring MiMe; screening homozygous MiMe material with genetic background recombination in these gregated offspring of heterozygous MiMe material after self-bred; by means of asexual propagation, expanding reproduction of homozygous MiMe with polyploid heterosis, induce and preserve homozygous MiMe by using Fix plants; double self-bred of homozygous MiMe material resulting in polyploid breeding material with heterosis. The invention is applied to combine polyploid breeding with heterosis, solve the problem that the utilization of polyploid heterosis must rely on polyploid induction and hybrid seed must be produced every season, and provide a simple and efficient plant breeding method of utilizing polyploid heterosis.
Disclosed is a method for rapidly predicting the initial heading stage of rice. The present invention belongs to the technical field of rice cultivation. The method in the present invention builds a mathematical model formula for the light temperature adaptability level of a rice variety, and quantizes the light temperature adaptability level of the rice variety by means of planting at three test points of different latitudes. The quantization allows for not only trans-regional prediction, but also cross-seedtime prediction, effectively improving prediction accuracy, saving much time and labor, and providing technical support for commercial breeding and market promotion of rice.
G06Q 10/04 - Forecasting or optimisation specially adapted for administrative or management purposes, e.g. linear programming or "cutting stock problem"
5.
A METHOD FOR INDUCING HAPLOIDS AND ITS APPLICATION IN PLANT BREEDING
The present invention relates to the field of biotechnology and plant breeding, and in particular relates to a method using plant cell proliferation regulator gene to induce haploid, and a use thereof in plant breeding. In the present invention, expression profiles of unfertilized egg cells of rice and rice embryos 5 days after fertilization are analyzed, and 2 genes are discovered to have relatively high expression, OsCPRO1 and OsCPRO2. The research shows that these two genes can be utilized to induce the production of haploid descendants. Specifically, a promoter specifically expressed in egg cells controls the gene to convert a plant, obtaining induced haploid plant material of a positive transgenic plant. Furthermore, the induced haploid plant material can be combined with a MiMe system to obtain apomixes material, and thereby generate clone seeds.
The present disclosure discloses use of a rice OsPR6 gene or a protein encoded thereby in regulating resistance of rice to Magnaporthe oryzae, and relates to the technical field of prevention and treatment of rice blast. A CDS nucleotide sequence of the rice OsPR6 gene is shown in SEQ ID NO. 2. A study finds that the rice gene OsPR6 plays an important role in a resistance process of rice to rice blast. The gene may be used for screening to obtain a rice plant line resistant to Magnaporthe oryzae, or the protein encoded thereby may be used as a drug for preventing and treating rice blast.
The present invention relates to the fields of biotechnology and plant breeding, and in particular relates to a method using a plant cell proliferation regulator gene to induce haploidy, and a use thereof in plant breeding. In the present invention, expression profiles of unfertilized egg cells of rice and rice embryos 5 days after fertilization are analyzed, and 2 genes are discovered to have relatively high expression, OsCPRO1 and OsCPRO2. The research shows that these two genes may be utilized to induce the production of haploid descendants. Specifically, a promoter specifically expressed by egg cells controls the gene to convert a plant, obtaining induced haploid plant material of a positive transgenic plant. Furthermore, the induced haploid plant material may be combined with a MiMe system to obtain apomictic material, and thereby generate a clone seed.
Disclosed in the present invention is a plant breeding method using polyploid heterosis. The method comprises the following steps: S1, mutating a MiMe key gene by using a technical means, screening strains with all gene loci being heterozygous, and creating a heterozygous MiMe material; S2, hybridizing the heterozygous MiMe material with other strains, and separating a new heterozygous MiMe strain from a progeny to achieve the purpose of a MiMe transfer strategy; S3, carrying out selfing separation on the heterozygous MiMe material, and screening a homozygous MiMe material with a recombinant genetic background; S4, carrying out expanding propagation on the homozygous MiMe with polyploidy heterosis in asexual propagation modes such as tillering, stem node culture and anther culture, carrying out mass seed propagation, and carrying out Fix induced preservation on the homozygous MiMe; and S5, carrying out selfing doubling on the homozygous MiMe material to generate a polyploid breeding material with heterosis. By means of applying the technical solution of the present invention, polyploid breeding and heterosis are combined, the problem of polyploid heterosis utilization necessarily depending on polyploid induction and hybrid seed production of each generation is solved, and a simple and efficient plant breeding method with polyploid heterosis is provided.
A organic fertilizer for controlling pollution or accumulation of heavy metal cadmium in rice and a preparation method and an application method thereof are related to the technical field of fertilizers. The fertilizer is, based on 100 kg of organic material with a water content of 15%, added with 0.05-5 kg of iron salt calculated as iron, and is also added with manganese salt, zinc salt and copper salt, wherein a mass ratio of the iron salt calculated as iron, the manganese salt calculated as manganese, the zinc salt calculated as zinc and the copper salt calculated as copper is 1:0.2-8:0.08-2:0.001-0.5.
A preparation method of a carbon-based selenium-enriched fertilizer for rice is disclosed. The preparation method of the carbon-based selenium-enriched fertilizer for rice includes planting a selenium-enriched plant material; collecting and concentrating a selenium-enriched extracting solution; preparing modified selenium-enriched biomass charcoal from the tertiary residues; and preparing the carbon-based selenium-enriched fertilizer. In the present disclosure, active organic selenium enriched and generated by plant is compounded with the biomass charcoal, so that the fixation and loss of the active selenium in soil are reduced, and the utilization efficiency of the selenium fertilizer is improved.
The present disclosure discloses a method for using plant heterosis. The method comprises the following steps: S1, transforming the meiosis of germ cells of hybrids into mitosis-like so as to obtain gametes whose genotype and chromosome ploidy are consistent with hybrids by using gene mutation or gene engineering technology; and S2, influencing and involving in the development of gametes or embryos in plants by using gene mutation and gene engineering technology, wherein a protein involved is MTL protein.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
12.
NOVEL ORGANIC FERTILIZER FOR CONTROLLING POLLUTION OR ACCUMULATION OF HEAVY METAL CADMIUM IN RICE, AND PREPARATION METHOD AND APPLICATION METHOD THEREFOR
A novel organic fertilizer for controlling the pollution or accumulation of heavy metal cadmium in rice, a preparation method and an application method therefor, which relate to the technical field of fertilizers. Using a baseline of 100 kg of organic material that has a water content of 15%, a fertilizer is added having 0.05 to 5 kg of an iron salt for iron, and manganese salt, zinc salt and copper salt are added according to the mass ratio of iron salt for iron: manganese salt for manganese: zinc salt for zinc: copper salt for copper being 1:0.2 to 8:0.08 to 2:0.001 to 0.5. The organic fertilizer is developed on the basis of the chemical process of heavy metal cadmium in rice paddy field soil, the absorption process of rice roots and the transfer process of plant cadmium. The fertilizer is convenient to apply, low in usage amount, low in price, and low in costs, is generally applicable to farmland that has heavy, medium and light cadmium pollution in China, and has wide adaptability.
Provided are a method and a kit for constructing a simplified genomic library. The method comprises: performing a non-specific amplification on a whole genome with a first pair of primers to obtain random amplified fragments; performing a specific amplification on the random amplified fragments with a second pair of primers to obtain the simplified genomic library.
Provided is a method using plant hybrid vigor, comprising the following steps: S1: transforming, using gene mutations or the genetic engineering technique, the meiosis of germ cells of hybrids into mitosis-like to obtain gametes of which the genotype and the chromosome ploidy are consistent to those of hybrids; and S2: influencing, using genetic mutations and the genetic engineering technique, the participation in a process of plant gamete or embryo development, wherein the protein involved is MTL protein.
A sequencing data result analysis method and apparatus, and a sequencing library construction and sequencing method. The sequencing data result analysis method comprises: acquiring a sequencing data result of a sequencing library (S101), with the sequencing library comprising a plurality of mixed samples, each of the samples corresponding to a label sequence combination, and different samples corresponding to different label sequence combinations, wherein each label sequence combination comprises a plurality of label sequences, the sequencing data result is a sequencing fragment set obtained by sequencing the plurality of mixed samples, and the sequencing fragment set comprises a plurality of disordered sequencing fragments; determining a label sequence combination of each of the sequencing fragments (S102); and determining, according to the label sequence combination of each of the sequencing fragments, a sample corresponding to each of the sequencing fragments (S103). The method solves the technical problems of a relatively low efficiency and relatively high costs caused by the artificial discrimination against samples needing to be performed by scientific and technological workers with a technical background for a sequencing data result in the relevant art.
G06F 19/22 - for sequence comparison involving nucleotides or amino acids, e.g. homology search, motif or Single-Nucleotide Polymorphism [SNP] discovery or sequence alignment
16.
METHOD AND TEST KIT FOR CONSTRUCTING SIMPLIFIED GENOMIC LIBRARY
Provided are a method and test kit for constructing a simplified genomic library. The method comprises: using a first pair of primers to perform non-specific amplification on a whole genome, and obtaining random amplified fragments; using a second pair of primers to perform specific amplification on the random amplified fragments, and obtaining a simplified genomic library.
Provided are a gene combination used for controlling foreign gene expression in specific plant tissue, and a method applying the gene combination to cultivate a transgenic plant. The method is used to cultivate, for example, an endosperm zero expression-type transgenic rice, i.e. rice grain endosperm produced by the rice does not contain any transgenic product protein synthesis and accumulation.
An activator for soil heavy metal and a preparation method therefor, which belong to the technical field of soil remediation. The activator is made from the following raw materials in parts by weight: 60-140 parts of an activating substance, 50-500 parts of a sulfur-containing compound, 0-1000 parts of an organic material of 10% of the water content, 0-500 parts of an alkaline substance calculated by sodium hydroxide, 0-500 parts of a reducing substance, and 0-200 parts of water. The activator has very strong bonding capability to heavy metals and extremely strong specificity. The contents of the heavy metals, in particular cadmium, copper, lead and mercury, in the soil solution are greatly improved after the activator has been applied to the soil, The arsenic content in the soil solution is greatly improved under flooded conditions, and the activator can be directly applied to phytoremediation and washing remediation of the soil heavy metals. During the application process, the fertilizer is simple to operate, has a low amount, a low price, and low use costs, and is generally applicable to Chinese dry lands polluted by cadmium to heavy, medium and light degrees.
The present invention provides a soil heavy metal curing agent for controlling accumulation of heavy metals of crops and its preparation method. The curing agent is made from the following parts of raw materials by weight: 60˜140 parts of substance containing carbon-carbon double bond; 1˜400 parts of sulfo-compound by sulfur; 50˜500 parts of organic matter by 10% water content; 0˜400 parts of water; 0˜100 parts of an initiator; 0˜200 parts of a reducer; and 0˜200 parts of a strong base. The curing agent for heavy metals in the soil according to the present invention can reduce the cadmium, lead and mercury content in the soil and further greatly reduce the roots' absorption of these heavy metals.
C09K 17/50 - Organic compounds mixed with inorganic active ingredients, e.g. polymerisation catalysts the organic compound being of natural origin, e.g. cellulose derivatives
C05G 3/00 - Mixtures of one or more fertilisers with additives not having a specifically fertilising activity
C05G 3/04 - Mixtures of one or more fertilisers with materials not having a specifically fertilising activity with soil conditioners
C09K 17/40 - Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
20.
Noninvasive method of source-sink regulation in rice
A noninvasive method of source-sink regulation in rice belongs to the technical field of rice production. In this method, the source-sink relationship is regulated by a rice sterile line and its identical type of maintaining line being subjected to mixed-planting and insulated pollination, or sowing and transplanting at different times and insulated pollination, so as to construct rice plant samples with gradient difference of source-sink levels. The present invention is a kind of native, natural noninvasive method of source-sink regulation, which could broaden the traditional thinking of source-sink theoretical research, especially overcome the deficiency in conventional methods such as leaf-cutting, spikelet-thinning that lead to physical injury or physiological interference. The method provides a brand new approach and solution for thoroughly investigating source-sink relationship in rice, wheat, maize and other crops, and will play an important role in enriching crop source-sink theory and also promoting the development of the related disciplines.
Provided are an artificially designed and modified insecticidal protein or a segment thereof. The insecticidal protein belongs to a Vip3A-type insecticidal protein. The protein or the segment thereof has an insecticidal activity against a pest, especially a lepidoptera pest. Also provided are a nucleic acid for coding the protein or the segment thereof, an insecticidal composition, a DNA constructor, and a transformed microorganism or a plant comprising the nucleic acid, and a method for controlling a pest, especially a plant pest.
The present invention provides a natural and non-harmful method for regulating and controlling a source and a library of rice. The library regulation and control are implemented by planting a sterile-series rice and a same-model maintenance-series rice thereof in a matching manner, or carrying out sowing in different periods and carrying out isolated pollination, so that rice plant samples whose sources and libraries have gradient difference horizontally are constructed. The present invention overcomes defects of physical injury or physiological interference exists in a leaf in cutting, ear cutting and other commonly used methods, and provides a new method and approach for deeply researching relationships of sources and libraries of crops such as rice, wheat and corns.
The present invention relates to the technical field of fertilizers. Disclosed are a soil heavy metal curing agent for controlling accumulation of heavy metals of crops and a preparation method thereof. The soil heavy metal curing agent is prepared by using the following raw materials in parts by weight: 60 to 140 parts of substances containing carbon-carbon double bonds, 1 to 400 parts of sulfocompounds calculated by sulfur, 50 to 500 parts of organic materials with water content of 10%, 0 to 400 parts of water, 0 to 100 parts of an initiator, 0 to 200 parts of a reducing agent, and 0 to 200 parts of alkali. The soil heavy metal curing agent of the present invention can reduce the content of cadmium, lead, and mercury in a soil solution, and greatly reduce absorption of roots on these heavy metals.
Disclosed are a soil replacement tray substrate for rice for raising seedlings and a method for raising seedlings thereof, belonging to the technical field of soil replacement tray substrates for raising seedlings for crops. The invention is characterized in that a pulp, an adhesive, a water-retaining agent and a fertilizer are included, with the amount of pulp being 100.0-1000.0 g/tray, the amount of adhesive being 0.1-2.0 g/tray, the amount of water-retaining agent being 2.0-20.0 g/tray, and the amount of nitrogen in the fertilizer being 0.5-5.0 g/tray, the amount of phosphorus in the fertilizer being 0.2-3.0 g/tray, and the amount of potassium in the fertilizer being 0.1-1.5 g/tray. The soil replacement tray substrate for rice for raising seedlings contains a pulp and a water-retaining agent, with the advantages of retaining water and fertility, preserving heat, good air permeability, a simple production process, and light weight of the end product, etc. The substrate is suitable for tray raising seedlings for a mechanical rice transplanter. The quality of the seedlings raised by the soil replacement substrate is superior to that of seedlings raised by traditional nutrient soil. Meanwhile, the cost of raising seedlings is significantly decreased, and the end product seedling tray is lighter so that the load and work strength of the transplanter is greatly lessened.
A seedling-raising and root-shaping agent for inserting into the plate of a rice seeding machine and application method thereof are disclosed. The root-shaping agent comprises an auxin-like plant growth regulator (with a content of 1-10 ppm) and sodium dichloroisocyanurate (with a content of 50-200 ppm). The root-shaping agent can promote the growth of the machine planted seedling, generate the tiller in advance and increase effective spike to dramatically improve the output of the rice; and has simple and convenient use including two application methods of soaking seeds and spraying.
A01N 43/40 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
A01N 47/40 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
A hybrid rice seed producing method includes steps as follows: (1) breeding small grain sterile line and corresponding hybrid rice with properties of Zhong Hua 11 small grain mutant (CGMCC No.2741); (2) hybridizing the small grain sterile line with large grain or normal grain maintaining line hybrid rice for seed producing; (3) carrying out mechanized seed producing by utilizing the small grain sterile line hybrid rice to perform mixing sowing and mixing harvesting; (4) screening and separating of seeds after mechanical harvest. By applying said method simplifies the seed producing procedures and reduces the cost of seed producing.
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