A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor relative the substrate to be determined from linear portions of the sinusoidal signal without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02K 11/00 - Structural association of dynamo-electric machines with electric components or with devices for shielding, monitoring or protection
H02K 11/215 - Magnetic effect devices, e.g. Hall-effect or magneto-resistive elements
H02K 15/02 - Processes or apparatus specially adapted for manufacturing, assembling, maintaining or repairing of dynamo-electric machines of stator or rotor bodies
H02K 21/22 - Synchronous motors having permanent magnetsSynchronous generators having permanent magnets with stationary armatures and rotating magnets with magnets rotating around the armatures, e.g. flywheel magnetos
H02K 29/08 - Motors or generators having non-mechanical commutating devices, e.g. discharge tubes or semiconductor devices with position sensing devices using magnetic effect devices, e.g. Hall-plates or magneto-resistors
2.
EXPONENTIAL BASE-3 AND GREATER NUCLEIC ACID AMPLIFICATION WITH REDUCED AMPLIFICATION TIME
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.
Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising modifying the aptamer by uracil-DNA glycosylase enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. In some aspects, the oligonucleotide aptamers are circular and comprise one or more deoxyuridine nucleotides providing for aptamer-specific recognition and modification of the circular aptamer by the uracil-DNA glycosylase enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing uracil-DNA glycosylase enzymatic activity are provided. The methods can be practiced using kits comprising a DNA polymerase-binding oligonucleotide aptamer and at least one uracil-DNA glycosylase enzymatic activity having oligonucleotide aptamer-specific recognition to provide for specific modification of the aptamer by the uracil-DNA glycosylase enzymatic activity.
Compositions, methods, and systems for detecting human epidermal growth factor receptor 2 (HER2)-low expression levels in a biological sample are disclosed. Also disclosed are compositions, methods, and systems for distinguishing HER2-low expression from HER2-negative or HER2-positive expression. Compositions for improved RNA recovery during sample preparation steps and detection of nucleic acids in the methods herein are also disclosed.
Described herein is a comprehensive novel amplification system that can employ novel nucleic acid constructs as blocked primers, such constructs including a target-specific region, wherein the target-specific region comprises a target-specific cleavage domain, and a universal adapter sequence located 5′ of the target-specific region, wherein the universal adapter sequence comprises a non-natural nucleotide base. Also described is a fusion protein between a polymerase and an endonuclease, which is useful in this system, as well as related methods and kits.
Thermal control devices utilizing a thermal switch to facilitate cooling of a biological sample, particularly a biological sample tested with a semiconductor diagnostic detection chip. Such thermal switches can include a mass of thermally conductive material, such as copper or aluminum, that selectively contacts the diagnostic chip or sample tube by use of an air cylinder or a servo-driven moveable support. Alternatively, the thermal switch can utilize a thermally conductive material that selectively contacts the diagnostic chip or sample tube by use of an inflatable bladder. Alternatively, the thermal switch can utilize a voice coil and a heat sink to selectively contact the diagnostic chip. The thermal control device can further include thermal overshoot feature, such as an air blower or placement of a heat sink in close proximity to the chip or tube. Associated methods of assembly and use of thermal control devices are also provided herein.
Thermal control devices utilizing a thermal switch to facilitate cooling of a biological sample, particularly a biological sample tested with a semiconductor diagnostic detection chip. Such thermal switches can include a mass of thermally conductive material, such as copper or aluminum, that selectively contacts the diagnostic chip or sample tube by use of an air cylinder or a servo-driven moveable support. Alternatively, the thermal switch can utilize a thermally conductive material that selectively contacts the diagnostic chip or sample tube by use of an inflatable bladder. Alternatively, the thermal switch can utilize a voice coil and a heat sink to selectively contact the diagnostic chip. The thermal control device can further include thermal overshoot feature, such as an air blower or placement of a heat sink in close proximity to the chip or tube. Associated methods of assembly and use of thermal control devices are also provided herein.
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Downloadable and recorded operating system software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; downloadable and recorded software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for collecting, managing, storing, analyzing, and reporting data generated by the apparatus and instruments; downloadable and recorded operating software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; downloadable and recorded software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; downloadable and recorded software for collecting, managing, storing, analyzing, and reporting biological and medical information; downloadable and recorded software used for collecting, managing, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; downloadable and recorded data analysis software for laboratory, scientific, research, and medical use; downloadable and recorded computer software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; downloadable and recorded laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; downloadable and recorded computer programs, namely, computer programs for medical monitoring and analysis of biological and medical data; downloadable and recorded software for clinical decision support; downloadable and recorded software for providing medical information to a clinician; downloadable and recorded software for data analysis and clinical decision support for health care; downloadable and recorded software using artificial intelligence for data analysis and clinical decision support for health care. Software as a service (SAAS) services featuring software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; Software as a service (SAAS) services featuring software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for uploading, collecting, managing, storing, analyzing, sharing, reporting, and preserving data generated by the apparatus and instruments; Software as a service (SAAS) services featuring software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; Software as a service (SAAS) services featuring software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; Software as a service (SAAS) services featuring software for collecting, managing, storing, analyzing, and reporting biological and medical information; Software as a service (SAAS) services featuring software for collecting, managing, aggregating, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; Software as a service (SAAS) services featuring data analysis software for laboratory, scientific, research, and medical use; Software as a service (SAAS) services featuring software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; Software as a service (SAAS) services featuring laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; Software as a service (SAAS) services featuring software for medical monitoring and analysis of biological and medical data; Providing temporary use of non-downloadable medical analysis software for use in medical diagnostics; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data to predict epidemiological trends; providing online non-downloadable software for clinical decision support; providing temporary use of non-downloadable software for providing medical information to a clinician; providing on-line non-downloadable software for data analysis and clinical decision support for health care; software as a service (SAAS) services featuring software using artificial intelligence for data analysis and clinical decision support for health care.
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Software; downloadable and recorded software; downloadable and recorded computer software platforms; software as a medical device [SaMD]; computer operating programs; downloadable and recorded operating system software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; downloadable and recorded software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for collecting, managing, storing, analyzing, and reporting data generated by the apparatus and instruments; downloadable and recorded operating software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; downloadable and recorded software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; downloadable and recorded software for collecting, managing, storing, analyzing, and reporting biological and medical information; downloadable and recorded software used for collecting, managing, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; downloadable and recorded data analysis software for laboratory, scientific, research, and medical use; downloadable and recorded computer software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; downloadable and recorded laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; downloadable and recorded computer programs, namely, computer programs for medical monitoring and analysis of biological and medical data; downloadable and recorded software for clinical decision support; downloadable and recorded software for providing medical information to a clinician; downloadable and recorded software for data analysis and clinical decision support for health care; downloadable and recorded software using artificial intelligence for data analysis and clinical decision support for health care (1) Software as a service [SaaS]; providing online non-downloadable computer software; platform as a service [PaaS]; computer software consultancy; computer software design; maintenance of computer software; updating of computer software; Software as a service (SAAS) services featuring software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; Software as a service (SAAS) services featuring software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for uploading, collecting, managing, storing, analyzing, sharing, reporting, and preserving data generated by the apparatus and instruments; Software as a service (SAAS) services featuring software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; Software as a service (SAAS) services featuring software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; Software as a service (SAAS) services featuring software for collecting, managing, storing, analyzing, and reporting biological and medical information; Software as a service (SAAS) services featuring software for collecting, managing, aggregating, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; Software as a service (SAAS) services featuring data analysis software for laboratory, scientific, research, and medical use; Software as a service (SAAS) services featuring software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; Software as a service (SAAS) services featuring laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; Software as a service (SAAS) services featuring software for medical monitoring and analysis of biological and medical data; Providing temporary use of non-downloadable medical analysis software for use in medical diagnostics; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data to predict epidemiological trends; providing online non-downloadable software for clinical decision support; providing temporary use of non-downloadable software for providing medical information to a clinician; providing on-line non-downloadable software for data analysis and clinical decision support for health care; software as a service (SAAS) services featuring software using artificial intelligence for data analysis and clinical decision support for health care
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
downloadable and recorded operating system software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; downloadable and recorded software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for collecting, managing, storing, analyzing, and reporting data generated by the apparatus and instruments; downloadable and recorded operating software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; downloadable and recorded software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; downloadable and recorded software for collecting, managing, storing, analyzing, and reporting biological and medical information; downloadable and recorded software used for collecting, managing, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; downloadable and recorded data analysis software for laboratory, scientific, research, and medical use; downloadable and recorded computer software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; downloadable and recorded laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; downloadable and recorded computer programs, namely, computer programs for medical monitoring and analysis of biological and medical data; downloadable and recorded software for clinical decision support; downloadable and recorded software for providing medical information to a clinician; downloadable and recorded software for data analysis and clinical decision support for health care; downloadable and recorded software using artificial intelligence for data analysis and clinical decision support for health care Software as a service (SAAS) services featuring software for use in connection with laboratory and diagnostic apparatus and instruments for analyzing biological samples and bodily fluids; Software as a service (SAAS) services featuring software for use in operating, monitoring, and controlling laboratory and diagnostic apparatus and instruments for uploading, collecting, managing, storing, analyzing, sharing, reporting, and preserving data generated by the apparatus and instruments; Software as a service (SAAS) services featuring software for use with laboratory and diagnostic instruments, namely, for use in providing automation of laboratory processes, control and monitoring of laboratory instruments, instrument operation, setting up experiment protocols, sample management, connectivity, and data, collection, analysis, management, and reporting; Software as a service (SAAS) services featuring software for technical support and advice, troubleshooting, diagnosis, repair, and maintenance of laboratory equipment and diagnostic instruments; Software as a service (SAAS) services featuring software for collecting, managing, storing, analyzing, and reporting biological and medical information; Software as a service (SAAS) services featuring software for collecting, managing, aggregating, storing, analyzing, and reporting data from laboratory and diagnostic apparatus and instruments; Software as a service (SAAS) services featuring data analysis software for laboratory, scientific, research, and medical use; Software as a service (SAAS) services featuring software using artificial intelligence for managing and analyzing data from laboratory and diagnostic instruments; Software as a service (SAAS) services featuring laboratory middleware software for providing an interface between laboratory, physician, hospital, and medical information systems and laboratory and diagnostic instruments; Software as a service (SAAS) services featuring software for medical monitoring and analysis of biological and medical data; Providing temporary use of non-downloadable medical analysis software for use in medical diagnostics; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data; Software as a service (SAAS) services featuring software for aggregating and analyzing biological data to predict epidemiological trends; providing online non-downloadable software for clinical decision support; providing temporary use of non-downloadable software for providing medical information to a clinician; providing on-line non-downloadable software for data analysis and clinical decision support for health care; software as a service (SAAS) services featuring software using artificial intelligence for data analysis and clinical decision support for health care
12.
THREE-PHASE NESTED AMPLIFICATION AND MULTI-PHASIC DETECTION
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
14.
INTEGRATED VALVE BODY AND SYRINGE TUBE COMPONENT FOR ASSAY CARTRIDGES
Integrated valve body and syringe tube components to facilitate controlled fluid flow in assay cartridges are provided herein. Such components can include a valve body and syringe tube that are integrally formed as a single component. Components can include a syringe tube having a conduit in fluid communication with a valve body having a filter cavity and set of ports to facilitate direct fluid flow between chambers and fluid flow through a filter in the filter cavity of the valve body. Valve body can include an off-center filter cavity and a direct flow path in fluid communication with a filter flow path disposed in a common plane and designed to inhibit cross-contamination between flow paths. Valve body can be sealed by a cap snap-fit or welded to the valve body or a thin film. Flow valves can also be included. Two-part welding stack improves control of filter compression and performance.
Integrated valve body and syringe tube components to facilitate controlled fluid flow in assay cartridges are provided herein. Such components can include a valve body and syringe tube that are integrally formed as a single component. Components can include a syringe tube having a conduit in fluid communication with a valve body having a filter cavity and set of ports to facilitate direct fluid flow between chambers and fluid flow through a filter in the filter cavity of the valve body. Valve body can include an off-center filter cavity and a direct flow path in fluid communication with a filter flow path disposed in a common plane and designed to inhibit cross-contamination between flow paths. Valve body can be sealed by a cap snap-fit or welded to the valve body or a thin film. Flow valves can also be included. Two-part welding stack improves control of filter compression and performance.
A semi-fluid dispensing device having an injection block with vent relief to facilitate improve dispensing of a semi-fluid, such as grease, to a component. The system can further include a metering assembly for precise metering of the semi-fluid to the component to avoid excess deposition and waste. The injection block can include multiple plates stacked together, and can include a middle injection plate having defined therein an injection conduit, injection flow channels extending around a through-hole that receives the component and a vent relief channel that allows air to exit the channels through a vent relief outlet as the semi-fluid is dispensed. The device can incorporate the in-line needle shut-off valve into an existing grease deposition equipment thereby providing compatibility with an existing footprint and assembly workflow.
Automated oligonucleotide synthesis-compatible sulforhodamine dye phosphoramidite compounds and labeled polynucleotides incorporating these dyes are provided.
A biological sample processing apparatus having an enclosure. A plurality of sample processing modules are held by the enclosure. Each sample processing module is configured to hold a removable sample cartridge and to only perform sample processing on a sample within the corresponding removable sample cartridge. Each sample processing module is configured to perform at least one of a plurality of testing processes on the sample within the removable sample cartridge. At least one module in the apparatus is configured to perform nucleic acid amplification and detection.
Methods, systems, and computer storage media may be used to detect and differentiate bacterial and viral infections. Cellular population parameters such as the monocyte distribution width (MDW) can be acquired for an individual. Clinical measurements such as a complete blood count (CBC), a measure of procalcitonin (PCT) or a measure of C-reactive protein (CRP) can be obtained for an individual. A trained model can be used to compute an output value from the parameters. The output value can be compared to a threshold value to determine a probability associated with a bacterial or viral infection. A report can be generated from the probability and sent to the appropriate party.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
G16H 15/00 - ICT specially adapted for medical reports, e.g. generation or transmission thereof
G16H 20/00 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance
G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indicesICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
20.
SYSTEMS AND METHODS FOR AI BASED INFECTION CLASSIFICATION
Methods, systems, and computer storage media may be used to detect and differentiate bacterial and viral infections. Cellular population parameters such as the monocyte distribution width (MDW) can be acquired for an individual. Clinical measurements such as a complete blood count (CBC), a measure of procalcitonin (PCT) or a measure of C-reactive protein (CRP)can be obtained for an individual. A trained model can be used to compute an output value from the parameters. The output value can be compared to a threshold value to determine a probability associated with a bacterial or viral infection. A report can be generated from the probability and sent to the appropriate party.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
G16H 15/00 - ICT specially adapted for medical reports, e.g. generation or transmission thereof
G16H 20/00 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance
G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indicesICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
The present disclosure provides pancoronavirus biomarker panels that can be used to detect a wide variety of characterized coronavirus strains and, in some embodiments, emerging or novel coronavirus strains. In certain embodiments, this detection can be carried out in a single, highly-multiplexed nucleic acid amplification assay.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
Thermal control devices and methods to provide improved control, speed and accuracy in temperature cycling of a fluid. Such devices and methods measure or estimate temperature of fluid non-invasively and in real-time by use of capacitance measurements of the fluid. Such systems include use of multiple electrodes positioned along the reaction-vessel, such that capacitance of the fluid is determined without contact, thereby preventing contamination of the fluid or sensors. The temperature or changes in temperature can be estimated based on capacitance in combination with a known or assumed relationship between temperature and the dielectric constant of the fluid. Optionally, the capacitance-based estimate can be improved by algorithmically combining with a measurement(s) from additional temperature sensor(s) in proximity to the reaction-vessel. Such systems and methods can be used for thermal cycling in biological processing and testing, as well as any industrial and chemical processes that can employ non-invasive fluid temperature monitoring.
The present disclosure provides a set of primers and optional probes for identifying the presence of α-coronavirus, β-coronavirus, SARS-COV-2, adenovirus, Chlamydia pneumoniae, Influenza A, Influenza B, metapneumovirus, rhinovirus/enterovirus, Mycoplasma, Bordetella spp., parainfluenza, and respiratory syncytial virus (RSV), which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
24.
ENCODERLESS MOTOR WITH IMPROVED QUANTIZATION AND METHODS OF USE AND CALIBRATION
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor to be determined from the sinusoidal signals by utilizing a transformation matrix or piece-wise algorithm applied in substantially linear portions of the sinusoidal signals without requiring use of additional hardware encoder or position sensors and without requiring noise-reduction or filtering of the signal.
A fluidic bridge device for transport of a fluid sample between a first sample processing device and a second sample processing device. The fluidic bridge may include one or more fluid channels extending between fluid-tight couplings attachable to transfer ports of the first and second sample processing device. In one aspect, the first device is a sample preparation device and the second device is an assay device. The fluidic bridge can include at least two fluid conduits, at least one for transport of the prepared sample, and at least one other to facilitate displacement of air to allow flow of the prepared sample through the other fluid conduit. The fluid channels can include one or more of an amplification chamber, a processing chamber, a gas-permeable vent, a bubble trap, a filter, and an external port. Methods of preparing and transporting a fluid sample between devices are provided herein.
Described herein are methods and compositions that provide highly efficient primer annealing and denaturation during nucleic acid amplification. The basic method employs destabilizing nucleotide triphosphates (dNTPs) in amplification reaction mixtures. The incorporation of such dNTPs into the amplicon produced upon amplification lowers the Tm of the amplicon, promoting denaturation/strand separation for the next round of primer annealing.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Described herein are methods and compositions that provide highly efficient primer annealing and denaturation during nucleic acid amplification. The basic method employs destabilizing nucleotide triphosphates (dNTPs) in amplification reaction mixtures. The incorporation of such dNTPs into the amplicon produced upon amplification lowers the Tm of the amplicon, promoting denaturation/strand separation for the next round of primer annealing.
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
The present disclosure provides a set of primers and optional probes for identifying the presence of α-coronavirus, β-coronavirus, SARS-CoV-2, adenovirus, Chlamydia pneumoniae, Influenza A, Influenza B, metapneumovirus, rhinovirus/enterovirus, mycoplasma, Bordetella spp., parainfluenza, and respiratory syncytial virus (RSV), which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
The present disclosure provides a set of primers and optional probes for identifying the presence of MPOX virus, Clade II, which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
The present disclosure provides a set of primers and optional probes for identifying the presence of MPOX virus, Clade II, which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
35.
MOTOR HAVING INTEGRATED ACTUATOR WITH ABSOLUTE ENCODER AND METHODS OF USE
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with a first set of permanent magnets distributed radially about the rotor to facilitate rotation of the rotor and a second set of permanent magnets on the rotor to facilitate determination of an absolute position of the rotor. The motor further includes first and second set of sensors for detection of the magnets of the inner and outer rings. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an absolute position of the rotor relative the substrate to be determined from the sinusoidal signals without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02P 7/025 - Arrangements for regulating or controlling the speed or torque of electric DC motors the DC motors being of the linear type the DC motors being of the moving coil type, e.g. voice coil motors
Methods and systems for detecting defect in sample cartridges in real-time during manufacturing. Such systems utilize one or more external sensors that detect characteristics or parameters of the sample cartridge and/or the manufacturing process from one or more data sets. The external sensor(s) include any of: an RGB camera, IR camera, high-resolution optical camera, and ultrasonic microphone or combination thereof. An automated system obtains data sets from external sensor(s) and compares the data sets to a baseline of the sample cartridge and/or manufacturing process such that defects can be determined based on a variance from the baseline. Such methods can utilize feature extraction and spectrum analysis to identify features or characteristics for comparison with the baseline. A machine learning model can be used to determine an algorithm based on data sets of acceptable sample cartridges and data sets from the external sensor(s) that are associated with the cartridge defect.
B23K 20/10 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating making use of vibrations, e.g. ultrasonic welding
G01N 21/90 - Investigating the presence of flaws, defects or contamination in a container or its contents
Methods and system for identifying faulty sample cartridges in real-time during manufacturing are provided herein. Such systems use monitored operational parameters associated with operation of manufacturing equipment that manufactures the sample cartridges. One or more data sets of operational parameters are obtained from existing equipment controls and/or additional sensors and compared to corresponding operational parameters associated with acceptable cartridges. A faulty detection unit can be configured to compare the operational parameters and can optionally include algorithms and/or models, such as a machine learning model, by which faulty cartridges can be identified. The comparison can include determining whether the monitored operational parameters are within a pre-defined range of acceptable values and/or deviate from a characteristic profile of the operational parameter in a manner indicative of faulty cartridges. The faulty cartridge detection can be integrated within automation controls so that faulty cartridges can be detected in real-time and automatically discarded during manufacturing.
Methods and systems for training a model for automated defect detection of a product during manufacturing are provided herein. Such methods utilize a combination of supervised transfer learning through auxiliary tasks and a combination of supervised and unsupervised learning. The methods can utilize supervised transfer learning with expert labels on a generalized auxiliary task, such as product classification, which is transferred to more specific auxiliary tasks, such as identification of specific product features and/or anomaly detection, where additional expert labels are then applied to the anomalies, and another iteration of supervised learning further improves the model. The anomalies can correspond to features associated with defects, which can be induced experimentally to improve efficiency of the training procedure. The product can be a sample cartridge such that the model allows detection of faulty cartridges based on sample cartridge and/or manufacturing process data.
Film seal assemblies that seal one or more reagents within a sample cartridge, which allow for single lid cartridge designs. Such film seal assemblies can include one or more layers, a bottom-most layer that permanently seals with a sample cartridge having multiple chambers for one or more reagents, and top-most layer that releasably seals vent opening(s) in the film seal assembly and is removable by the end user. This configuration allows use of a single lid, which can be integrally formed with cartridge body or a separate component attached atop the cartridge body. The film seal assembly can include peelable liner(s) or can releasably attached to an underside of the single lid. Such film assemblies can include multiple layers that include a polypropylene layer for permanently sealing to a rigid propylene cartridge body and polyester top layers for releasably and hermetically sealing with a liner or the lid.
INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulfonating the bound deaminated nucleic acid and/or simultaneously eluting and desulfonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.
C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
42.
FILM SEAL ASSEMBLY AND SINGLE LID ASSAY CARTRIDGE AND ASSOCIATED METHODS
Film seal assemblies that seal one or more reagents within a sample cartridge, which allow for single lid cartridge designs. Such film seal assemblies can include one or more layers, a bottom-most layer that permanently seals with a sample cartridge having multiple chambers for one or more reagents, and top-most layer that releasably seals vent opening(s) in the film seal assembly and is removable by the end user. This configuration allows use of a single lid, which can be integrally formed with cartridge body or a separate component attached atop the cartridge body. The film seal assembly can include peelable liner(s) or can releasably attached to an underside of the single lid. Such film assemblies can include multiple layers that include a polypropylene layer for permanently sealing to a rigid propylene cartridge body and polyester top layers for releasably and hermetically sealing with a liner or the lid.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B65D 17/50 - Non-integral frangible members applied to, or inserted in, preformed openings, e.g. tearable strips or plastic plugs
B65D 51/16 - Closures not otherwise provided for with means for venting air or gas
B65D 51/22 - Caps, lids, or covers co-operating with an inner closure arranged to be opened by piercing, cutting, or tearing having means for piercing, cutting, or tearing the inner closure
B65D 53/08 - Flexible adhesive strips adapted to seal filling or discharging apertures
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. The method employs pairs of primers that differ significantly in Tm and a novel temperature/time course characterized by a temperature pulse during denaturation that enables a high-Tm primer (but not a low-Tm primer) to anneal and prime the synthesis of an additional nucleic acid strand beyond the two strands synthesized in a cycle of classical PCR. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
Methods and systems for detecting defect in sample cartridges in real-time during manufacturing. Such systems utilize one or more external sensors that detect characteristics or parameters of the sample cartridge and/or the manufacturing process from one or more data sets. The external sensor(s) include any of: an RGB camera, IR camera, high-resolution optical camera, and ultrasonic microphone or combination thereof. An automated system obtains data sets from external sensor(s) and compares the data sets to a baseline of the sample cartridge and/or manufacturing process such that defects can be determined based on a variance from the baseline. Such methods can utilize feature extraction and spectrum analysis to identify features or characteristics for comparison with the baseline. A machine learning model can be used to determine an algorithm based on data sets of acceptable sample cartridges and data sets from the external sensor(s) that are associated with the cartridge defect.
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B29C 65/08 - Joining of preformed partsApparatus therefor by heating, with or without pressure using ultrasonic vibrations
Methods and system for identifying faulty sample cartridges in real-time during manufacturing are provided herein. Such systems use monitored operational parameters associated with operation of manufacturing equipment that manufactures the sample cartridges. One or more data sets of operational parameters are obtained from existing equipment controls and/or additional sensors and compared to corresponding operational parameters associated with acceptable cartridges. A faulty detection unit can be configured to compare the operational parameters and can optionally include algorithms and/or models, such as a machine learning model, by which faulty cartridges can be identified. The comparison can include determining whether the monitored operational parameters are within a pre-defined range of acceptable values and/or deviate from a characteristic profile of the operational parameter in a manner indicative of faulty cartridges. The faulty cartridge detection can be integrated within automation controls so that faulty cartridges can be detected in real-time and automatically discarded during manufacturing.
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]
47.
TRANSFER LEARNING METHODS AND MODELS FACILITATING DEFECT DETECTION
Methods and systems for training a model for automated defect detection of a product during manufacturing are provided herein. Such methods utilize a combination of supervised transfer learning through auxiliary tasks and a combination of supervised and unsupervised learning. The methods can utilize supervised transfer learning with expert labels on a generalized auxiliary task, such as product classification, which is transferred to more specific auxiliary tasks, such as identification of specific product features and/or anomaly detection, where additional expert labels are then applied to the anomalies, and another iteration of supervised learning further improves the model. The anomalies can correspond to features associated with defects, which can be induced experimentally to improve efficiency of the training procedure. The product can be a sample cartridge such that the model allows detection of faulty cartridges based on sample cartridge and/or manufacturing process data.
Automated oligonucleotide synthesis-compatible sulforhodamine dye phosphoramidite compounds and labeled polynucleotides incorporating these dyes are provided.
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having a well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.
Compositions and methods for isolating and detecting nucleic acid in a biological sample are provided. The compositions and methods utilize a modified solid support comprising an amine or amide group.
Systems for processing a fluid sample to facilitate analysis with a semiconductor detection chip are provided herein. Such systems can include a sample processing cartridge coupleable with a chip carrier device configured for transport of the processed fluid sample from the sample cartridge. The chip carrier device can include one or more fluid channels extending between fluid-tight couplings attachable to transfer ports of the sample processing cartridge. The chip carrier device can include multiple portions or adapters, including a fluid sample portion, a flowcell portion and a chip carrier. Also provided are methods of preparing and transporting a fluid sample from a sample cartridge into a chip carrier device for analysis with a semiconductor detection chip carried within the chip carrier device.
Test cartridges for performing multi-target assay panels, in particular multiplex vaginal panels (MVP) and methods of use and manufacture. Such cartridges can include a cartridge body having multiple chambers and rotatable valve assembly to facilitate controlled fluid flow, and various reagents and buffers to chemically lyse nucleic acids from multiple targets in MVP. Specialized valve assemblies have been utilized to resist alkaline lysing buffers required to chemically lyse multiple targets, including various protozoa, yeasts and bacteria, including Atopobium spp. (Atopobium vaginae, Atopobium novel species CCUG 55226); Bacterial Vaginosis-Associated Bacterium 2 (BVAB2); and Megasphaera-1. Methods include detecting levels of each respective target in a single sample from a single multi-target assay cartridge and identifying the subject as having bacterial vaginosis, vulvovaginal candidiasis (or the presence of Candida spp.), or trichomoniasis (or presence of Trichomonas vaginalis) based on detected levels of each of: Atopobium spp., BVAB2, Megasphaera-1, Candida spp., and Trichomonas vaginalis.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
53.
Encoderless motor with improved granularity and methods of use
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor relative the substrate to be determined from linear portions of the sinusoidal signal without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02K 11/00 - Structural association of dynamo-electric machines with electric components or with devices for shielding, monitoring or protection
H02K 11/215 - Magnetic effect devices, e.g. Hall-effect or magneto-resistive elements
H02K 15/02 - Processes or apparatus specially adapted for manufacturing, assembling, maintaining or repairing of dynamo-electric machines of stator or rotor bodies
H02K 29/08 - Motors or generators having non-mechanical commutating devices, e.g. discharge tubes or semiconductor devices with position sensing devices using magnetic effect devices, e.g. Hall-plates or magneto-resistors
H02K 21/22 - Synchronous motors having permanent magnetsSynchronous generators having permanent magnets with stationary armatures and rotating magnets with magnets rotating around the armatures, e.g. flywheel magnetos
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
55.
NUCLEIC ACID EXTRACTION AND ISOLATION WITH HEAT LABILE SILANES AND CHEMICALLY MODIFIED SOLID SUPPORTS
Compositions and methods for isolating and detecting nucleic acid in a biological sample are provided. The compositions and methods utilize a modified solid support comprising an amine or amide group.
Improved sample cartridges, valve assemblies and methods of manufacture and assembly are provided herein. Such systems can include a sample processing cartridge having a unitary cartridge body with integral syringe tube and valve interface. Such systems can further includes valve assemblies with an overmolded gasket and gaskets with a protruding conical valve sealing surface. Various additional features can include thin film sealing for cartridge as well as valve assemblies for chemical lysis. Thin film sealing for cartridge lids can include various multi-layered designs to facilitate injection and sealing of reagents within the cartridge. Magnetic separation features are also included. Such features can be included in various design iterations as needed for compatibility with existing technologies and to accommodate needs for manufacturing workflows.
Diagnostic detection chip device designs that reduce cost of fabrication and assembly are described herein. Such chip device designs include features that facilitate use of the chip within a chip carrier device with integrated fluid flow control features and compatibility with conventional sample cartridges and sample processing systems. Associated methods of manufacture and assembly of the chip devices are also provided herein.
Improved sample cartridges, valve assemblies and methods of manufacture and assembly are provided herein. Such systems can include a sample processing cartridge having a unitary cartridge body with integral syringe tube and valve interface. Such systems can further includes valve assemblies with an overmolded gasket and gaskets with a protruding conical valve sealing surface. Various additional features can include thin film sealing for cartridge as well as valve assemblies for chemical lysis. Thin film sealing for cartridge lids can include various multi-layered designs to facilitate injection and sealing of reagents within the cartridge. Magnetic separation features are also included. Such features can be included in various design iterations as needed for compatibility with existing technologies and to accommodate needs for manufacturing workflows.
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. The method employs pairs of primers that differ significantly in Tm and a novel temperature/time course characterized by a temperature pulse during denaturation that enables a high-Tm primer (but not a low-Tm primer) to anneal and prime the synthesis of an additional nucleic acid strand beyond the two strands synthesized in a cycle of classical PCR. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.
RFID-based operations management systems and methods are provided herein, particularly RFID based Kanban systems. Such system can realize increased efficiencies, improved visibility of inventory throughout the workflow and improved response to variations in demand. RFID based solutions can automatically transact consumption of Kanban inventory in real-time and trigger replenishment signals to warehouses automatically, resulting in increased Kanban inventory accuracy and lower manual efforts for replenishment and consumption posting. This approach can interface with existing operational management software (e.g. ERP systems, such as SAP) seamlessly integrating with product manufacturing workflows. Such systems are uniquely applicable to manufacture of biological testing kits, where demands fluctuate considerably in response to crises, disasters, epidemics and pandemics.
G06Q 10/087 - Inventory or stock management, e.g. order filling, procurement or balancing against orders
G06K 19/07 - Record carriers with conductive marks, printed circuits or semiconductor circuit elements, e.g. credit or identity cards with integrated circuit chips
A remote monitoring system for medical data collection can include a data-flagging process embeds authorization and settings information into a file containing the test results. Such data flagging can occur at a medical device or testing site, and may be based in policy settings received from a remote system. A file containing the test results can also include data category information that can be used to protect sensitive information by preventing such information from being communicated to the wrong server.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
H04L 67/12 - Protocols specially adapted for proprietary or special-purpose networking environments, e.g. medical networks, sensor networks, networks in vehicles or remote metering networks
G16H 40/40 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the management of medical equipment or devices, e.g. scheduling maintenance or upgrades
G16H 40/67 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for remote operation
62.
PSEUDO-COMPLEMENTARY BASES IN GENOTYPING AND NUCLEIC ACID SEQUENCING
Described herein are methods and compositions that make use of pseudo-complementary bases to reduce unwanted hybridization in assays to detect and/or quantify particular nucleotide sequences, as well as in nucleic acid sequencing protocols.
Improved sub-assemblies and methods of control for use in a diagnostic assay system adapted to receive an assay cartridge are provided herein. Such sub-assemblies include: a brushless DC motor, a door opening/closing mechanism and cartridge loading mechanism, a syringe and valve drive mechanism assembly, a sonication horn, a thermal control device and optical detection/excitation device. Such systems can further include a communications unit configured to wirelessly communicate with a mobile device of a user so as to receive a user input relating to functionality of the system with respect to an assay cartridge received therein and relaying a diagnostic result relating to the assay cartridge to the mobile device.
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 33/487 - Physical analysis of biological material of liquid biological material
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
H02P 6/182 - Circuit arrangements for detecting position without separate position detecting elements using back-emf in windings
H02P 27/06 - Arrangements or methods for the control of AC motors characterised by the kind of supply voltage using variable-frequency supply voltage, e.g. inverter or converter supply voltage using DC to AC converters or inverters
64.
COMPOSITIONS AND METHODS OF DIAGNOSING AND TREATING TUBERCULOSIS
Compositions and methods for detecting Mycobacterium tuberculosis (MTB) infection in a patient suspected of being infected with MTB and for distinguishing active tuberculosis (ATB), incipient tuberculosis (ITB) or subclinical tuberculosis (STB) from latent tuberculosis and other pulmonary and infectious diseases are provided. The methods may also be used to monitor treatment responses of MTB infected patients. Changes in the expression level of genes are used to aid in the diagnosis, prognosis, and treatment of tuberculosis.
Compositions and methods for detecting mycobacterium tuberculosis (MTB) infection in a patient suspected of being infected with MTB and for distinguishing active tuberculosis (ATB), incipient tuberculosis (ITB) or subclinical tuberculosis (STB) from latent tuberculosis and other pulmonary and infectious diseases are provided. The methods may also be used to monitor treatment responses of MTB infected patients. Changes in the expression level of genes are used to aid in the diagnosis, prognosis, and treatment of tuberculosis.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
66.
Exponential base-3 and greater nucleic acid amplification with reduced amplification time
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.
Described herein is a comprehensive novel amplification system that can employ novel nucleic acid constructs as blocked primers, such constructs including a target-specific region, wherein the target-specific region comprises a target-specific cleavage domain, and a universal adapter sequence located 5' of the target-specific region, wherein the universal adapter sequence comprises a non-natural nucleotide base. Also described is a fusion protein between a polymerase and an endonuclease, which is useful in this system, as well as related methods and kits.
Systems and methods for monitoring, characterizing and controlling operation of LEDs are provided herein. Methods includes measuring a voltage across the LED, and correlating the voltage to a junction temperature of the LED. This correlation can be used to improve operation of the LED by increasing the signal to noise ratio of the LED signal, characterize the LED by comparing to an I-V curve, control LED operation to compensate for LED degradation and avoid crosstalk, and/or to generally improve performance and life expectancy of the LED. Improved performance of the LED can include stabilizing the photon output during performance of an assay to provide a desired dye reporter signal required for the assay and/or reducing an intra-shot during of the LED output during the assay. System and device with control units configured to perform these methods are also described herein.
Systems and methods for monitoring, characterizing and controlling operation of LEDs are provided herein. Methods include measuring a voltage (V) across a LED (1), and correlating the voltage (V) to a junction temperature of the LED (1). This correlation can be used to improve operation of the LED (1) by increasing the signal to noise ratio of the LED signal (5), characterize the LED (1) by comparing to an l-V curve, control LED operation to compensate for LED degradation and avoid crosstalk, and/or to generally improve performance and life expectancy of the LED (1). Improved performance of the LED (1) can include stabilizing the photon output (5) during performance of an assay to provide a desired dye reporter signal required for the assay and/or reducing an intra-shot during of the LED output (5) during the assay. System and device with control units (2) configured to perform these methods are also described herein.
Sample cartridge, valve assembly and processing methods for providing mechanical lysis, chemical lysis or both for a given fluid sample are provided herein. Such systems can include a sample processing cartridge having a valve assembly configured for transport of the processing of fluid sample within the sample cartridge. The valve assembly can include a valve body and cap that secure a filter therebetween and facilitate inflow of mechanical or chemical lysing agents as needed for a fluid sample. Assay workflows for performing both mechanical and chemical lysis of a fluid sample within the same workflow of a single universal sample cartridge are also provided.
Reaction vessels, cartridges, devices and methods for facilitating high-level multiplexing are described herein. Such reaction vessels can include a planar frame defining a fluidic path between a first planar substrate and a second planar substrate, a fluidic interface is located at one end of the planar frame with a pair of fluidic ports, a well chamber and a pre-amplification chamber. Devices for spotting reagents in wells of high-level multiplexing reaction vessels and improved reagent solutions are also described herein.
Reaction vessels, cartridges, devices and methods for facilitating high-level multiplexing are described herein. Such reaction vessels can include a planar frame defining a fluidic path between a first planar substrate and a second planar substrate, a fluidic interface is located at one end of the planar frame with a pair of fluidic ports, a well chamber and a pre-amplification chamber. Devices for spotting reagents in wells of high-level multiplexing reaction vessels and improved reagent solutions are also described herein.
Sample cartridge, valve assembly and processing methods for providing mechanical lysis, chemical lysis or both for a given fluid sample are provided herein. Such systems can include a sample processing cartridge having a valve assembly configured for transport of the processing of fluid sample within the sample cartridge. The valve assembly can include a valve body and cap that secure a filter therebetween and facilitate inflow of mechanical or chemical lysing agents as needed for a fluid sample. Assay workflows for performing both mechanical and chemical lysis of a fluid sample within the same workflow of a single universal sample cartridge are also provided.
Sample cartridge, valve assembly and processing methods for providing mechanical lysis, chemical lysis or both for a given fluid sample are provided herein. Such systems can include a sample processing cartridge having a valve assembly configured for transport of the processing of fluid sample within the sample cartridge. The valve assembly can include a valve body and cap that secure a filter therebetween and facilitate inflow of mechanical or chemical lysing agents as needed for a fluid sample. Assay workflows for performing both mechanical and chemical lysis of a fluid sample within the same workflow of a single universal sample cartridge are also provided.
The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.
A lid apparatus for a multi-chambered container. The lid apparatus has a top-lid that is hingedly attached to a bottom-cap. The top-lid includes one or more openings for fluid filling multiple passages that extend from the bottom-cap. A lower bottom-cap includes welding features for welding to the multi-chambered container. The bottom-cap further includes one or more auxiliary ports for injecting a reagent when the lid apparatus is in a closed configuration sealingly attached to the multi-chambered sample container.
B65D 47/08 - Closures with discharging devices other than pumps with pouring spouts or tubesClosures with discharging devices other than pumps with discharge nozzles or passages having articulated or hinged closures
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
The present disclosure is directed to methods and kits for discriminating between a bacterial infection and viral infection in a human subject. More specifically, the methods can comprise detecting the expression level of a combination of ABL1, IRF9, ITGAM, LY6E, PSTPIP2 and RUNX1 in biological samples from the human subject and determining whether the human subject has a bacterial or viral infection based on those expression levels.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
80.
METHODS FOR DISCRIMINATING BACTERIAL AND VIRAL INFECTIONS IN A HUMAN SUBJECT
The present disclosure is directed to methods and kits for discriminating between a bacterial infection and viral infection in a human subject. More specifically, the methods can comprise detecting the expression level of a combination of ABL1, IRF9, ITGAM, LY6E, PSTPIP2 and RUNX1 in biological samples from the human subject and determining whether the human subject has a bacterial or viral infection based on those expression levels.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
Thermal control devices and methods to provide improved control, speed and efficiency in temperature cycling are provided herein. Such thermal control device and methods can include one or more active elements, such a thermoelectric cooler device, that is controlled by an algorithm that regulates a temperature distribution of an adjacent reaction-vessel according to a temperature distribution command trajectory and estimated reaction-vessel temperature distribution. Some embodiments include two active elements that are bilaterally applied to opposing sides of the reaction-vessel. In some embodiments, the estimated reaction-vessel temperature is determined based on a state of power electronics of the element and a temperature output of one or more sensors of a portion of the element and/or an ambient environment of the reaction-vessel. Methods of calibration of such systems utilizing a thermal calibrator as a proxy for the reaction-vessel are also provided herein.
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
F25B 21/02 - Machines, plants or systems, using electric or magnetic effects using Peltier effectMachines, plants or systems, using electric or magnetic effects using Nernst-Ettinghausen effect
G01K 1/02 - Means for indicating or recording specially adapted for thermometers
G01K 13/024 - Thermometers specially adapted for specific purposes for measuring temperature of moving fluids or granular materials capable of flow of moving gases
G01N 1/42 - Low-temperature sample treatment, e.g. cryofixation
G01N 1/44 - Sample treatment involving radiation, e.g. heat
84.
LOSSY MECHATRONIC SYSTEMS AND METHODS OF ESTIMATION
Methods and systems for estimating force and motor torque in a mechatronic system are provided herein. Such methods and system are suited for improved control of small-scale mechatronic system, particularly a syringe, valve, and cartridge loader or door opening/closing mechanism of a diagnostic assay system. The methods can compensate for friction and account for various second-order effects, thereby allowing for more accurate pressure estimation, thereby allowing improved syringe operation. The methods can further allow for improved estimation of force or motor torque to allow for improved control of an actuatable valve interfacing the sample cartridge and cartridge loader or door opening/closing system. Methods of calibrating such systems are also provided.
Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising cleaving the aptamer by endonuclease V enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. The oligonucleotide aptamers of the present invention are circular and comprise one or more deoxyinosine nucleotides providing for aptamer-specific recognition and cleavage of the circular aptamer by the endonuclease V enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing endonuclease V enzymatic activity are provided. The methods can be practiced using kits comprising a DNA polymerase-binding oligonucleotide aptamer and at least one endonuclease V enzymatic activity having oligonucleotide aptamer-specific recognition to provide for specific cleavage of the aptamer by the endonuclease V enzymatic activity.
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising modifying the aptamer by uracil-DNA glycosylase enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. In some aspects, the oligonucleotide aptamers are circular and comprise one or more deoxyuridine nucleotides providing for aptamer-specific recognition and modification of the circular aptamer by the uracil-DNA glycosylase enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing uracil-DNA glycosylase enzymatic activity are provided. The methods can be practiced using kits comprising a DNA polymerase-binding oligonucleotide aptamer and at least one uracil-DNA glycosylase enzymatic activity having oligonucleotide aptamer-specific recognition to provide for specific modification of the aptamer by the uracil-DNA glycosylase enzymatic activity.
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
Methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulphonating the bound deaminated nucleic acid and/or simultaneously eluting and desulphonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.
Thermal control devices and methods to provide improved control, speed and efficiency in temperature cycling are provided herein. Such thermal control device and methods can include one or more active elements, such a thermoelectric cooler device, that is controlled by an algorithm that regulates a temperature distribution of an adjacent reaction-vessel according to a temperature distribution command trajectory and estimated reaction-vessel temperature distribution. Some embodiments include two active elements that are bilaterally applied to opposing sides of the reaction-vessel. In some embodiments, the estimated reaction-vessel temperature is determined based on a state of power electronics of the element and a temperature output of one or more sensors of a portion of the element and/or an ambient environment of the reaction-vessel. Methods of calibration of such systems utilizing a thermal calibrator as a proxy for the reaction-vessel are also provided herein.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
90.
LOSSY MECHATRONIC SYSTEMS AND METHODS OF ESTIMATION
Methods and systems for estimating force and motor torque in a mechatronic system are provided herein. Such methods and system are suited for improved control of small-scale mechatronic system, particularly a syringe, valve, and cartridge loader or door opening/closing mechanism of a diagnostic assay system. The methods can compensate for friction and account for various second-order effects, thereby allowing for more accurate pressure estimation, thereby allowing improved syringe operation. The methods can further allow for improved estimation of force or motor torque to allow for improved control of an actuatable valve interfacing the sample cartridge and cartridge loader or door opening/closing system. Methods of calibrating such systems are also provided.
An n phase encoder for use in a mechatronic system, including a movable element (104, 304) that applies a magnetic field with period S representing a total displacement, and a stationary support (201, 310) with n magnetic field sensors (202a-c, 312) mounted thereon and positioned on the stationary support (201, 310) so as to measure the magnetic field imparted by the movable element (104, 304), wherein n is greater than 1, the encoder being configured to obtain signals from the n magnetic field sensors (202a-c, 312) corresponding to the measurements of the magnetic field imparted by the movable element (104, 304), and to process n signals from the n magnetic field sensors (202a-c, 312) by utilizing a transformation matrix to determine a displacement of the movable element (104, 304), as well as associated signal processing and calibration methods.
G01D 5/244 - Mechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using electric or magnetic means influencing characteristics of pulses or pulse trainsMechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using electric or magnetic means generating pulses or pulse trains
G01D 5/14 - Mechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage
G01D 5/245 - Mechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using electric or magnetic means influencing characteristics of pulses or pulse trainsMechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using electric or magnetic means generating pulses or pulse trains using a variable number of pulses in a train
92.
ENCODERLESS MOTOR WITH IMPROVED QUANTIZATION AND METHODS OF USE AND CALIBRATION
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor to be determined from the sinusoidal signals by utilizing a transformation matrix or piece-wise algorithm applied in substantially linear portions of the sinusoidal signals without requiring use of additional hardware encoder or position sensors and without requiring noise-reduction or filtering of the signal.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Diagnostic reagents for scientific use; reagents and kits comprised primarily of reagents for scientific and research use in connection with the amplification, analysis, labeling and detection of nucleic acids. Medical diagnostic reagents; reagents and kits comprised primarily of reagents for medical, clinical, medical laboratory or medical diagnostic use in connection with the amplification, analysis, labeling and detection of nucleic acids. Laboratory equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples, nucleic acid amplifiers and optical detectors; laboratory equipment, namely, instrument systems, comprised of apparatus for nucleic acid amplification, analysis, labeling and detection, software for use in preparing and processing biological samples, cartridges, sample preparation land reaction containers and reaction vessels for preparing and processing biological samples, sold as a unit; apparatus for nucleic acid amplification, analysis, labeling and detection; laboratory equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection; laboratory devices for detecting genetic sequences; diagnostic apparatus for the detection of pathogens for laboratory or research use; scientific and research apparatus and instruments, namely, genetic sequencers for use in genetic analysis; apparatus for DNA and RNA testing for scientific research purposes; data processing apparatus. Clinical and medical equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples, nucleic acid amplifiers and optical detectors; clinical and medical equipment for diagnostic purposes, namely, instrument systems, comprised of apparatus for nucleic acid amplification, analysis, labeling and detection, software for use in preparing and processing biological samples, cartridges, sample preparation and reaction containers and reaction vessels for preparing and processing biological samples, sold as a unit; clinical and medical equipment for diagnostic purposes, namely, apparatus for nucleic acid amplification, analysis, labeling and detection; clinical and medical equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection; clinical and medical diagnostic apparatus and instruments for detecting genetic sequences; clinical and medical diagnostic apparatus for use in detecting pathogens; clinical and medical diagnostic apparatus and instruments for use in genetic analysis; apparatus for DNA and RNA testing for medical purposes; clinical and medical diagnostic apparatus and instruments for the analysis of body fluids.
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Providing a website featuring scientific and technological information and advice in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; scientific and technological consulting services in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment. Providing a website featuring medical information and advice in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment and laboratory equipment; medical consulting services in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; medical diagnostic testing, monitoring and reporting services.
95.
DIAGNOSTIC ASSAY SYSTEM WITH REPLACEABLE PROCESSING MODULES AND REMOTE MONITORING
A biological sample processing apparatus having an enclosure and a plurality of sample processing modules held within an enclosure with a tiltable graphical user interface screen. In one aspect, the individual modules that are readily removable for repair, replacement or upgrade. Each module is configured to be independently operable and readily inserted into the enclosure for connection with a processing unit of the enclosure. Each module can include quick-release mechanisms so that the module can be readily removed and replaced manually or with minimal tools through the front of the enclosure without requiring substantial or total disassembly of the module or entire enclosure. In another aspect, the user interface screen can display identifying information, such as a barcode, that can be scanned by a user's portable device so as to monitor the progress of an assay remotely.
A biological sample processing apparatus having an enclosure and a plurality of sample processing modules held within an enclosure with a tiltable graphical user interface screen. In one aspect, the individual modules that are readily removable for repair, replacement or upgrade. Each module is configured to be independently operable and readily inserted into the enclosure for connection with a processing unit of the enclosure. Each module can include quick-release mechanisms so that the module can be readily removed and replaced manually or with minimal tools through the front of the enclosure without requiring substantial or total disassembly of the module or entire enclosure. In another aspect, the user interface screen can display identifying information, such as a barcode, that can be scanned by a user's portable device so as to monitor the progress of an assay remotely.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
(1) Diagnostic reagents for scientific use; reagents and kits comprised primarily of reagents for scientific and research use in connection with the amplification, analysis, labeling and detection of nucleic acids
(2) Medical diagnostic reagents; reagents and kits comprised primarily of reagents for medical, clinical, medical laboratory or medical diagnostic use in connection with the amplification, analysis, labeling and detection of nucleic acids
(3) Laboratory equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples, nucleic acid amplifiers and optical detectors; Laboratory equipment, namely, instrument systems, comprised of apparatus for nucleic acid amplification, analysis, labeling and detection, software for use in preparing and processing biological samples, cartridges, sample preparation land reaction containers and reaction vessels for preparing and processing biological samples, sold as a unit; apparatus for nucleic acid amplification, analysis, labeling and detection; Laboratory equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection; Laboratory devices for detecting genetic sequences; Diagnostic apparatus for the detection of pathogens for laboratory or research use; Scientific and research apparatus and instruments, namely, genetic sequencers for use in genetic analysis; Apparatus for DNA and RNA testing for scientific research purposes; Data processing apparatus
(4) Clinical and medical equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples, nucleic acid amplifiers and optical detectors; Clinical and medical equipment for diagnostic purposes, namely, instrument systems, comprised of apparatus for nucleic acid amplification, analysis, labeling and detection, software for use in preparing and processing biological samples, cartridges, sample preparation and reaction containers and reaction vessels for preparing and processing biological samples, sold as a unit; Clinical and medical equipment for diagnostic purposes, namely, apparatus for nucleic acid amplification, analysis, labeling and detection; Clinical and medical equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection; Clinical and medical diagnostic apparatus and instruments for detecting genetic sequences; Clinical and medical diagnostic apparatus for use in detecting pathogens; Clinical and medical diagnostic apparatus and instruments for use in genetic analysis; Apparatus for DNA and RNA testing for medical purposes; Clinical and medical diagnostic apparatus and instruments for the analysis of body fluids (1) Providing a website featuring information and advice in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; Consulting services in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; Analysis and evaluation of the goods of others to assure compliance with standards, industry standards, and objectively determinable standards; Analysis and evaluation of the services of others to assure compliance with standards, industry standards, and objectively determinable standards
(2) Providing a website featuring information and advice in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; Consulting services in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; Medical diagnostic testing, monitoring and reporting services
98.
Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising cleaving the aptamer by endonuclease V enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. In some aspects, the oligonucleotide aptamer comprises one or more deoxyinosine nucleotides providing for aptamer-specific recognition and cleavage of the aptamer by the endonuclease V enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing endonuclease V enzymatic activity are provided. The methods can be practiced using kits comprising a DNA polymerase-binding oligonucleotide aptamer and at least one endonuclease V enzymatic activity having oligonucleotide aptamer-specific recognition to provide for specific cleavage of the aptamer by the endonuclease V enzymatic activity.
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Diagnostic reagents for scientific use; reagents and kits
comprised primarily of reagents for scientific and research
use in connection with the amplification, analysis, labeling
and detection of nucleic acids. Medical diagnostic reagents; reagents and kits comprised
primarily of reagents for medical, clinical, medical
laboratory or medical diagnostic use in connection with the
amplification, analysis, labeling and detection of nucleic
acids. Laboratory equipment, namely, a device for bioanalysis
incorporating a cartridge for sample preparation, nucleic
acid purification, nucleic acid amplification and optical
detection; laboratory equipment, namely, reaction vessels,
fluidic blocks for preparing and processing biological
samples, cartridges for preparing and processing biological
samples. Medical equipment, namely, a device for bioanalysis
incorporating a cartridge for sample preparation, nucleic
acid purification, nucleic acid amplification and optical
detection; medical equipment, namely, reaction vessels,
fluidic blocks for preparing and processing biological
samples, cartridges for preparing and processing biological
samples.
100.
METHODS OF DIAGNOSING TUBERCULOSIS AND DIFFERENTIATING BETWEEN ACTIVE AND LATENT TUBERCULOSIS
Compositions and methods for detecting mycobacterium tuberculosis (MTB) infection in a patient suspected of being infected with mycobacterium tuberculosis and for distinguishing between active and latent tuberculosis infection are provided. The methods may also be used to monitor progression of MTB infection or to monitor treatment of MTB infected patients. Changes in the expression level of genes are used to aid in the diagnosis, prognosis and treatment of tuberculosis.
