42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Data analysis services in the field of agriculture and biofuels; collection, compilation, analysis and management of data pertaining to agriculture, crops, and biofuels.
(2) Diagnostic services in the field of agriculture, crops, and biofuels; agricultural services, namely, crop data collection, crop data sampling, and crop observation for analysis purposes.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Business data analysis services in the field of agriculture and biofuels; collection, compilation, analysis and management of business data pertaining to agriculture, crops, and biofuels Diagnostic services in the nature of scientific research services in the field of agriculture, crops, and biofuels; agricultural services, namely, crop data collection, crop data sampling, and crop observation for scientific analysis purposes
3.
METHODS FOR QUANTIFYING STARCH AND CELLULOSE IN CORN SAMPLES
Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
Methods and solutions are disclosed for the quantification of mixed-linkage beta-glucan in plant biomass samples. Improved alkaline pretreatment, enzymatic hydrolysis, and glucose measurement parameters are provided that increase the accuracy, precision, and sensitivity of mixed-linkage beta-glucan assays.
C12Q 1/54 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving glucose or galactose
Methods and solutions are disclosed for the quantification of mixed-linkage beta-glucan in plant biomass samples. Improved alkaline pretreatment, enzymatic hydrolysis, and glucose measurement parameters are provided that increase the accuracy, precision, and sensitivity of mixed-linkage beta-glucan assays.
C12Q 1/54 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving glucose or galactose
The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.
Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
G01N 33/487 - Physical analysis of biological material of liquid biological material
9.
Cellulosic enzyme recycling from separation of saccharified biomass
The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Engineering services, namely, engineering in the field of cellulosic ethanol; scientific and technological services in the field of ethanol production, namely, scientific research, analysis and consultation in the field of ethanol production; performance of chemical analyses
42 - Scientific, technological and industrial services, research and design
Goods & Services
Engineering services, namely, engineering in the field of cellulosic ethanol; scientific and technological services in the field of ethanol production, namely, scientific research, analysis and consultation in the field of ethanol production; performance of chemical analyses
12.
High-solids biomass slurry generation for enhanced efficiency hydrolysis processing and equipment design to yield the same
This disclosure provides a high solids biomass slurry that is readily pumpable and transportable to downstream processing units, such as chemical and/or biochemical processing units. The slurry is amenable to saccharification efficiencies of >70 % in processing times of <36 hours. Also provided are devices for processing materials, such as the high solids biomass slurry.
Described herein are beta-glucosidase enzymes that have improved beta-glucosidase activity compared to a control beta-glucosidase enzyme. The improved beta-glucosidase enzymes are useful for converting a cellulosic biomass to fermentable sugars such as glucose. Also described are isolated polynucleotides that encode polypeptides having improved beta-glucosidase activity, expression cassettes for expressing the improved beta-glucosidase polypeptides, and cells, such as yeast cells, transformed with the expression cassettes.
The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.
The present disclosure provides methods and systems for generating sugars from biomass. The methods and systems provide increased yields of carbohydrates and fermentable sugars by combining enzyme recycling with removal of saccharification enzyme inhibitors and increased solids loading.
Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.
Improved haa1 transcriptional regulatory proteins, polynucleotides encoding improved haa1 transcriptional regulatory proteins and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved haa1 transcriptional regulatory proteins and cells expressing heterologous haa1 transcriptional regulatory proteins as disclosed herein.
Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.
Improved manganese peroxidases, polynucleotides encoding improved manganese peroxidase and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved manganese peroxidases and cells expressing a heterologous manganese peroxidase as disclosed herein.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12P 19/00 - Preparation of compounds containing saccharide radicals
C12P 7/14 - Multiple stages of fermentationMultiple types of microorganisms or reuse for microorganisms
C12N 9/08 - Oxidoreductases (1.), e.g. luciferase acting on hydrogen peroxide as acceptor (1.11)
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
[ Fermentation of corn to produce ethanol for others; production of bio-fuels and alternative fuels for others; ] consulting in the field of bio-fuel and alternative fuel production
22.
Recovery of desired co-products from fermentation stillage streams
Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.
The present invention provides for combinations of enzymes and other proteins that result in improved saccharification of plant material. The invention provides for saccharification in the presence of and optional fermentation by, yeast cells expressing the enzymes and other proteins.
This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process.
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
(1) Fermentation of corn to produce ethanol for others; development and production of bio-fuels and alternative fuels; consulting in the field of bio-fuels and alternative fuels.
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
(1) Fermentation of corn to produce ethanol for others; development and production of bio-fuels and alternative fuels; consulting in the field of bio-fuels and alternative fuels.
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
[ Fermentation of corn to produce ethanol for others; production of bio-fuels and alternative fuels for others; ] consulting in the field of bio-fuel and alternative fuel production
