01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes and enzyme preparations and compositions for
industrial purposes, particularly for the food, beverage and
starch industries; biochemical catalysts; biological
preparations, other than for medical or veterinary purposes;
ferments for chemical purposes; beer-clarifying and
preserving agents; filtering preparations for the beverages
industry; starch-liquifying chemicals [ungluing agents].
A bleaching process of pulp is disclosed comprising performing a delignification of the pulp by carrying out in a following sequence: an enzymatic treatment step comprising contacting the pulp with a glycoside hydrolase family GH11 xylanase enzyme at a pH of at least 9.5 and a temperature of at least 85° C., an oxidation step wherein a bleaching agent is added, an alkaline extraction step wherein an alkaline agent is added, and recovering bleached pulp, wherein the GH11 xylanase enzyme has an ability to cause at least 20% reduction in viscosity of a xylan-containing mixture at 90° C. and pH 10.5, compared to the same mixture without any xylanase enzyme.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes and enzyme preparations and compositions for industrial purposes; enzymes [other than for medical or veterinary use]; biochemical catalysts; biological preparations, other than for medical or veterinary purposes; ferments for chemical purposes; enzymes and enzyme preparations for use in drinks; enzymes and enzyme preparations for use in fermentation; enzymes and enzyme preparations for use in flavouring; enzymes and enzyme preparations for use in foodstuffs; enzymes and enzyme preparations for use in the bakery industry; enzymes and enzyme preparations for use in the dairy industry; enzymes and enzyme preparations for use in the detergent industry; enzymes and enzyme preparations for use in the food industry; enzymes and enzyme preparations for use in the textile industry; enzymes and enzyme preparations for use in the pulp and paper industry; enzymes and enzyme preparations for use in the manufacture of animal feed; enzymes for animal feed; enzymes and enzyme preparations in solid form or liquid form for industrial use; enzymes and enzyme preparations for use in food processing; enzymes and enzyme preparations for cloth processing; enzymes and enzyme preparations for food or drinks; enzymes and enzyme preparations for industrial purposes; enzymes and enzyme preparations for industrial use; enzymes and enzyme preparations for the bakery industry; enzymes and enzyme preparations for the beverage industry; enzymes and enzyme preparations for the brewing industry; enzymes and enzyme preparations for the detergent industry; enzymes and enzyme preparations for the food industry; enzymes and enzyme preparations for technical use; enzymes and enzyme preparations for scientific purposes; enzymes and enzyme preparations for scientific and research purposes.
The present invention relates to a xylanase polypeptide comprising (i) an amino acid sequence at least 70% identical to SEQ ID NO: 1, (ii) an amino acid exchange at the position corresponding to amino acid D11 in SEQ ID NO: 1; and (iii) an amino acid exchange at the position corresponding to amino acid S22 in SEQ ID NO: 1. The present invention further relates to polynucleotides, host cells, methods, compositions, and uses related thereto.
The present invention relates to a method of producing a dough, comprising (i) admixing a lactic acid producing microorganism and a propionic acid producing microorganism to flour and water; and (ii) incubating the admixture of step (i) for a first incubation period, wherein the lactic acid producing microorganism produces lactic acid and the propionic acid producing microorganism produces propionic acid, wherein the lactic acid producing microorganism and the propionic acid producing microorganism are capable of growing in a medium consisting of 50% (w/w) flour in water. The present invention also relates to a composition, preferably a starter culture for bakery good production, comprising viable cells of a lactic acid producing microorganism and viable cells of a propionic acid producing microorganism, wherein the lactic acid producing microorganism and the propionic acid producing microorganism are capable of growing in a medium consisting of 50% (w/w) flour in water. The present invention also relates to uses, baked goods, and methods related to the aforesaid method and composition.
The present invention relates to a method for converting sucrose comprised in a liquid food product into at least one fructooligosaccharide (FOS), the method comprising contacting said liquid food product with an Aspergillus niger fructosyltransferase under conditions allowing enzymatic conversion of sucrose to at least one FOS by said fructosyltransferase, wherein said contacting is performed at least until the sucrose concentration in said liquid food product is at most 15g/L; and to compositions, uses, and products related thereto.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes and enzyme preparations and compositions for
industrial purposes; enzymes [other than for medical or
veterinary use]; biochemical catalysts; biological
preparations, other than for medical or veterinary purposes;
ferments for chemical purposes; enzymes and enzyme
preparations for use in drinks; enzymes and enzyme
preparations for use in fermentation; enzymes and enzyme
preparations for use in flavouring; enzymes and enzyme
preparations for use in foodstuffs; enzymes and enzyme
preparations for use in the bakery industry; enzymes and
enzyme preparations for use in the dairy industry; enzymes
and enzyme preparations for use in the detergent industry;
enzymes and enzyme preparations for use in the food
industry; enzymes and enzyme preparations for use in the
textile industry; enzymes and enzyme preparations for use in
the pulp and paper industry; enzymes and enzyme preparations
for use in the manufacture of animal feed; enzymes for
animal feed; enzymes and enzyme preparations in liquid form
for industrial use; enzymes and enzyme preparations for use
in food processing; enzymes and enzyme preparations for
cloth processing; enzymes and enzyme preparations for food
or drinks; enzymes and enzyme preparations for industrial
purposes; enzymes and enzyme preparations for industrial
use; enzymes and enzyme preparations for the bakery
industry; enzymes and enzyme preparations for the beverage
industry; enzymes and enzyme preparations for the brewing
industry; enzymes and enzyme preparations for the detergent
industry; enzymes and enzyme preparations for the food
industry; enzymes and enzyme preparations for technical use;
enzymes and enzyme preparations for scientific purposes;
enzymes and enzyme preparations for scientific and research
purposes.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
(1) Enzymes and enzyme preparations and compositions for industrial purposes; enzymes [other than for medical or veterinary use]; biochemical catalysts; biological preparations, other than for medical or veterinary purposes; ferments for chemical purposes; enzymes and enzyme preparations for use in drinks; enzymes and enzyme preparations for use in fermentation; enzymes and enzyme preparations for use in flavouring; enzymes and enzyme preparations for use in foodstuffs; enzymes and enzyme preparations for use in the bakery industry; enzymes and enzyme preparations for use in the dairy industry; enzymes and enzyme preparations for use in the detergent industry; enzymes and enzyme preparations for use in the food industry; enzymes and enzyme preparations for use in the textile industry; enzymes and enzyme preparations for use in the pulp and paper industry; enzymes and enzyme preparations for use in the manufacture of animal feed; enzymes for animal feed; enzymes and enzyme preparations in liquid form for industrial use; enzymes and enzyme preparations for use in food processing; enzymes and enzyme preparations for cloth processing; enzymes and enzyme preparations for food or drinks; enzymes and enzyme preparations for industrial purposes; enzymes and enzyme preparations for industrial use; enzymes and enzyme preparations for the bakery industry; enzymes and enzyme preparations for the beverage industry; enzymes and enzyme preparations for the brewing industry; enzymes and enzyme preparations for the detergent industry; enzymes and enzyme preparations for the food industry; enzymes and enzyme preparations for technical use; enzymes and enzyme preparations for scientific purposes; enzymes and enzyme preparations for scientific and research purposes.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes and enzyme preparations and compositions for industrial purposes; biochemical catalysts; enzymes and enzyme preparations for use in drinks; enzymes and enzyme preparations for use in flavouring; enzymes and enzyme preparations for use in foodstuffs; enzymes and enzyme preparations for use in the bakery industry; enzymes and enzyme preparations for use in the dairy industry; enzymes and enzyme preparations for use in the detergent industry; enzymes and enzyme preparations for use in the food industry; enzymes and enzyme preparations for use in the textile industry; enzymes and enzyme preparations for use in the pulp and paper industry; enzymes and enzyme preparations for use in the animal feed industry; enzymes for use in the animal feed industry; enzymes and enzyme preparations in liquid form for industrial use; enzymes and enzyme preparations for cloth processing; enzymes and enzyme preparations for food or drinks; enzymes and enzyme preparations for industrial purposes; enzymes and enzyme preparations for industrial use; enzymes and enzyme preparations for the bakery industry; enzymes and enzyme preparations for the beverage industry; enzymes and enzyme preparations for the brewing industry; enzymes and enzyme preparations for the detergent industry
A bleaching process of pulp is disclosed comprising perform¬ ing a delignification of the pulp by carrying out in a following sequence: an enzymatic treatment step comprising contacting the pulp with a glycoside hy¬ drolase family GH11 xylanase enzyme at a pH of at least 9.5 and a tempera¬ ture of at least 85 °C, an oxidation step wherein a bleaching agent is added, an alkaline extraction step wherein an alkaline agent is added, and recovering bleached pulp, wherein the GH11 xylanase enzyme has an ability to cause at least 20 % reduction in viscosity of a xylan-containing mixture at 90°C and pH 10.5, compared to the same mixture without any xylanase enzyme.
A bleaching process of pulp is disclosed comprising performing a delignification of the pulp by carrying out in a following sequence: an enzymatic treatment step comprising contacting the pulp with a glycoside hydrolase family GH11 xylanase enzyme at a pH of at least 9.5 and a temperature of at least 85 °C, an oxidation step wherein a bleaching agent is added, an alkaline extraction step wherein an alkaline agent is added, and recovering bleached pulp, wherein the GH11 xylanase enzyme has an ability to cause at least 20 % reduction in viscosity of a xylan-containing mixture at 90°C and pH 10.5, compared to the same mixture without any xylanase enzyme.
The invention relates to the use of a protein having nuclease activity for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth, which comprises intact microorganisms after termination of the fermentation process. The invention moreover relates to a method for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth comprising intact microorganisms after fermentation and comprising a step of introducing a protein having nuclease activity into the fermentation broth during the fermentation until the start of recovering the end product. The invention further relates to fermentation processes comprising said use of a protein having nuclease activity to allow more flexibility to the downstream processing of the fermentation broth.
The present invention relates to a xylanase polypeptide comprising (i) an amino acid sequence at least 70% identical to SEQ ID NO:1, (ii) an amino acid exchange at the position corresponding to amino acid D11 in SEQ ID NO:1; and (iii) an amino acid exchange at the position corresponding to amino acid S22 in SEQ ID NO:1. The present invention further relates to polynucleotides, host cells, methods, compositions, and uses related thereto.
The present invention relates to a xylanase polypeptide comprising (i) an amino acid sequence at least 70% identical to SEQ ID NO:1, (ii) an amino acid exchange at the position corresponding to amino acid D11 in SEQ ID NO:1; and (iii) an amino acid exchange at the position corresponding to amino acid S22 in SEQ ID NO:1. The present invention further relates to polynucleotides, host cells, methods, compositions, and uses related thereto.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes and enzyme preparations and compositions for industrial purposes; enzymes [other than for medical or veterinary use]; biochemical catalysts; biological preparations, other than for medical or veterinary purposes; ferments for chemical purposes; enzymes and enzyme preparations for use in drinks; enzymes and enzyme preparations for use in fermentation; enzymes and enzyme preparations for use in flavouring; enzymes and enzyme preparations for use in foodstuffs; enzymes and enzyme preparations for use in the bakery industry; enzymes and enzyme preparations for use in the dairy industry; enzymes and enzyme preparations for use in the detergent industry; enzymes and enzyme preparations for use in the food industry; enzymes and enzyme preparations for use in the textile industry; enzymes and enzyme preparations for use in the pulp and paper industry; enzymes and enzyme preparations for use in the manufacture of animal feed; enzymes for animal feed; enzymes and enzyme preparations in liquid form for industrial use; enzymes and enzyme preparations for use in food processing; enzymes and enzyme preparations for cloth processing; enzymes and enzyme preparations for food or drinks; enzymes and enzyme preparations for industrial purposes; enzymes and enzyme preparations for industrial use; enzymes and enzyme preparations for the bakery industry; enzymes and enzyme preparations for the beverage industry; enzymes and enzyme preparations for the brewing industry; enzymes and enzyme preparations for the detergent industry; enzymes and enzyme preparations for the food industry; enzymes and enzyme preparations for technical use; enzymes and enzyme preparations for scientific purposes; enzymes and enzyme preparations for scientific and research purposes.
The present invention relates to a method of producing a fermented oil preparation from an oil preparation, said method comprising (a) admixing at least one food- and/or feed-grade microorganism to said oil preparation; and (b) thereby producing a fermented oil preparation; and to compositions, methods, organic products, and microorganisms related thereto.
C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
17.
USE OF GH12 CELLULASES IN PREPARING BAKERY PRODUCTS COMPRISING RYE-FLOUR
The invention relates to the use of a composition comprising an effective amount of at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives for improving at least one property of a dough comprising rye flour and/or of a bakery product prepared from said dough as well as to a method of preparing a bakery product based on rye having at least one improved property, said method comprising (i) providing a dough that has been added an effective amount of at least one polypeptide having GH12 cellulase activity or a composition comprising at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives, and rye flour, (ii) baking the dough for a time and temperature sufficient to yield the bakery product as well as to a baking enzyme composition for preparing rye-based bakery products comprising a polypeptide having a GH12 cellulase activity optionally together with one or more dough(s) or bakery product additives.
The present invention relates to a method of producing a dough, comprising (i) admixing a lactic acid producing microorganism and a propionic acid producing microorganism to flour and water; and (ii) incubating the admixture of step (i) for a first incubation period, wherein the lactic acid producing microorganism produces lactic acid and the propionic acid producing microorganism produces propionic acid, wherein the lactic acid producing microorganism and the propionic acid producing microorganism are capable of growing in a medium consisting of 50% (w/w) flour in water. The present invention also relates to a composition, preferably a starter culture for bakery good production, comprising viable cells of a lactic acid producing microorganism and viable cells of a propionic acid producing microorganism, wherein the lactic acid producing microorganism and the propionic acid producing microorganism are capable of growing in a medium consisting of 50% (w/w) flour in water. The present invention also relates to uses, baked goods, and methods related to the aforesaid method and composition.
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Ingredients used in the manufacture of foodstuffs, including
bakery goods (terms considered too vague by the
International Bureau - rule 13 (2) (b) of the Regulations);
cell cultures for use as ingredients for bakery goods. Food leavening agents.
20.
USE OF A NUCLEASE FOR REDUCING THE VISCOSITY AND/OR PREVENTING AN INCREASE IN VISCOSITY OF A FERMENTATION BROTH
The invention relates to the use of a protein having nuclease activity for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth, which comprises intact microorganisms after termination of the fermentation process. The invention moreover relates to a method for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth comprising intact microorganisms after fermentation and comprising a step of introducing a protein having nuclease activity into the fermentation broth during the fermentation until the start of recovering the end product. The invention further relates to fermentation processes comprising said use of a protein having nuclease activity to allow more flexibility to the downstream processing of the fermentation broth.
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
(1) Ingredients used in the manufacture of foodstuffs and bakery goods, namely enzymes for use in the bakery industry, enzymes for use in the food industry; cell cultures for use as ingredients for bakery goods, namely bacterial cultures for the bakery industry; food leavening agents, namely bacterial cultures for the food industry.
(2) Food leavening agents, namely, leaven and yeast.
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Ingredients used in the manufacture of foodstuffs and bakery goods, namely, microorganism cultures for the production of baked goods; cell cultures for use as ingredients for bakery goods Food leavening agents
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Ingredients used in the manufacture of foodstuffs, including bakery goods; cell cultures for use as ingredients for bakery goods. Food leavening agents.
24.
USE OF GH12 CELLULASES IN PREPARING BAKERY PRODUCTS COMPRISING RYE-FLOUR
The invention relates to the use of a composition comprising an effective amount of at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives for improving at least one property of a dough comprising rye flour and/or of a bakery product prepared from said dough 5 as well as to a method of preparing a bakery product based on rye having at least one improved property, said method comprising (i) providing a dough that has been added an effective amount of at least one polypeptide having GH12 cellu- lase activity or a composition comprising at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives,10 and rye flour, (ii) baking the dough for a time and temperature sufficient to yield the bakery product as well as to a baking enzyme composition for preparing rye- based bakery products comprising a polypeptide having a GH12 cellulase activity optionally together with one or more dough(s) or bakery product additives.
The invention relates to the use of a composition comprising an effective amount of at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives for improving at least one property of a dough comprising rye flour and/or of a bakery product prepared from said dough 5 as well as to a method of preparing a bakery product based on rye having at least one improved property, said method comprising (i) providing a dough that has been added an effective amount of at least one polypeptide having GH12 cellu- lase activity or a composition comprising at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives,10 and rye flour, (ii) baking the dough for a time and temperature sufficient to yield the bakery product as well as to a baking enzyme composition for preparing rye- based bakery products comprising a polypeptide having a GH12 cellulase activity optionally together with one or more dough(s) or bakery product additives.
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Enzymes and enzyme preparations and compositions for industrial purposes, particularly for the food and baking industries; biochemical catalysts; biological preparations, other than for medical or veterinary purposes; ferments for chemical purposes; flour containing enzymes for industrial purposes. Flour; flour mixes containing enzymes; flour mixtures containing enzymes for use in baking.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes and enzyme preparations and compositions for industrial purposes, particularly for the food, beverage and starch industries; biochemical catalysts; biological preparations, other than for medical or veterinary purposes; ferments for chemical purposes; beer-clarifying and preserving agents; filtering preparations for the beverages industry; starch-liquifying chemicals [ungluing agents].
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes and enzyme preparations and compositions for industrial purposes, particularly for the food and beverage industries; biochemical catalysts; biological preparations, other than for medical or veterinary purposes; ferments for chemical purposes; beer-clarifying and preserving agents; filtering preparations for the beverages industry; oil-degumming preparations.
29.
Polypeptides having phytase activity and the encoding nucleic acids
E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.
The invention relates to a process for identifying gene(s)/genetic element(s) associated with mating impairment in strains of Trichoderma reesei QM6a or strains derived thereof comprising the steps of a) providing a first strain being a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, b) sexually crossing said strain with a second strain being a mating competent strain of a Trichoderma reesei (Hypocrea jecorina) strain having a complementary locus, i.e. the MAT1-1 locus, c) repeatedly back-crossing the MAT1-1 progenies from the crossing of b) or the back-crossing thereof with the first strain of a), until a strain is obtained that is substantially identical to the first Trichoderma reesei QM6a strain or a strain derived thereof, but carries the MAT1-1 locus and is mating competent for crossing with Trichoderma reesei QM6a or any of its MAT1-2 progeny, d) selecting the progeny from step c) that is mating competent for crossing with a Trichoderma reesei (Hypocrea jecorina) having a MAT1-2 locus, and e) identifying the gene(s)/genetic element(s) associated with mating impairment by comparing the genome of the progenies selected in step d) with the genome sequences of the first strain of a) whereby said gene(s)/genetic element(s) may be fully or partially missing or existing in a mutated form or in a form having deletions or insertions in the first strain thus being a gene or a genetic element directly or indirectly associated with mating impairment in strains of Trichoderma reesei QM6a or a strain derived thereof as well as to a process for correcting the mating impairment of a Trichoderma reesei QM6a strain or a strain derived thereof having a MAT1-1 locus and that is not competent to mate with a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, wherein one or more mutated or fully or partially missing gene(s) and/or genetic element(s) identified as above is/are replaced by or complemented with the corresponding functional gene(s) and/or genetic element(s). Moreover, the invention relates to the use of a thus obtained fungal strain of the genus Trichoderma in industrial breeding and production of a product of interest. Moreover, the invention relates to the genes associated with mating impairment of Trichoderrna reesei QM6a and strains derived therefrom and to genes essential for mating of Trichoderma reesei QM6a and strains derived therefrom.
The invention relates to the use of a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having β-1,3- endoglucanase activity in a process of lysing yeast cell walls as well as to a process for lysing yeast cell walls which comprises exposing said yeast cell walls to a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having β-1,3-endoglucanase activity.
Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.
Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: 2 or a sequence derived therefrom, which may be obtained by substitution, addition, deletion of one or more amino acid(s), c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide which is coded for by a nucleic acid sequence which hybridizes under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acid(s), f) allelic variants to amino acid sequences a) to e).
D06M 16/00 - Biochemical treatment of fibres, threads, yarns, fabrics or fibrous goods made from such materials, e.g. enzymatic
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Enzymes, enzyme preparations as well as mixtures thereof for
enhancing the properties of cereal-based flours, dough,
bakery products and baked goods, in particular for improving
the shelf life of cereal-based bakery products and baked
goods. Cereal-based flours and dough, and bakery products and baked
goods made thereof, all containing enzymes, with enhanced
properties, in particular with an improved shelf life.
36.
CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES
The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
(1) Enzymes, enzyme preparations as well as mixtures thereof for enhancing the properties of cereal-based flours, dough, bakery products and baked goods, namely, for improving the shelf life, crumb softness, freshness, texture, flavor and taste of cereal-based bakery products and baked goods.
(2) Cereal-based flours and dough, and bakery products and baked goods made thereof, all containing enzymes, with enhanced properties, namely, with an improved shelf life, crumb softness, freshness, texture, flavor and taste.
The invention relates to a method for recovering of oil from plant seeds characterized in that a) an aqueous solution containing one or more cellulolytic and/or lipolytic and/or pectinolytic and/or proteolytic enzyme(s) and/or phytase is sprayed onto the seed, b) the thus obtained seed is directly supplied to a one-stage or multistage pressing in a way known per se, optionally coupled to an extraction, and c) the oil is recovered in a way known per se and optionally further processed, and the use of the method, particularly in the production of edible oil or biodiesel.
The invention relates to the use of one or more pectinolytic enzyme(s) for the treatment of fruit or vegetable mash as well as a process for enzymatic treatment of fruit or vegetable mash comprising the step of adding one or more pectinolytic enzyme(s), wherein at least one pectinolytic enzyme is obtainable from Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.
The invention relates to the use of one or more pectinolytic enzyme(s) for the treatment of fruit or vegetable mash as well as a process for enzymatic treatment of fruit or vegetable mash comprising the step of adding one or more pectinolytic enzyme(s), wherein at least one pectinolytic enzyme is obtainable from Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.
E. coli phytase sequence, wherein at least one amino acid at position 200 or position 207 is mutated as compared to the wild-type sequence, where the recombinant DNA molecule is, upon expression in a suitable host cell, associated with an increased activity of the thus encoded protein in the culture supernatant.
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
43.
EXPRESSION SYSTEM USED FOR THE ANTIBIOTIC-FREE PRODUCTION OF POLYPEPTIDES
The invention relates to an expression system for the production of one or more target polypeptide/target polypeptides, comprising a host cell in whose genome the DNA sequence that codes glycerine-3-phosphate dehydrogenase is inactivated or partially or completely deleted and which is transformed by an extrachromosomal element that comprises a DNA sequence that codes the target polypeptide(s) and glycerine-3-phosphate dehydrogenase, whereby not only the host cell genome but also the extrachromosomal element do not carry an antibiotic-resistance gene, as well as a DNA sequence that codes for a polypeptide with glycerine-3-phosphate dehydrogenase activity characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise a nucleotide sequence represented by the nucleotides 1338 to 2375 of SEQ ID NO: 1, c) DNA sequences that are coded by the plasmid pTP01 with the plasmid map according to Figure 4 and deposited under the deposition number DSM 18890, d) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA/nucleotide sequences according to a), b), c), d) or e) due to the degeneracy of the genetic code, and g) complementary strands to the sequences according to a) to f), and the protein coded thereby.
The invention relates to an expression system for the production of one or several target polypeptide(s), comprising a host cell, in whose genome the DNA sequence coding the glycerine-3-phosphate dehydrogenase is inactivated or partially or fully deleted and which is transformed with an extrachromosomal element that comprises a DNA Sequence coding the target polypeptide(s) and the glycerine-3-phosphate dehydrogenase. According to the invention, both the host cell genome and the extrachromosomal element do not carry any antibiotic-resistant gene. The invention also relates to a DNA sequence coding for a polypeptide with glycerine-3-phosphate dehydrogenase activity, characterized in that the DNA sequence is selected from a) DNA sequences comprising a nucleotide sequence according to SEQ ID NO:1; b) DNA sequences comprising a nucleotide sequence represented by the nucleotides 1338 to 2375 of SEQ ID NO:1; c) DNA sequences coded by the plasmid pTP01 with the plasmid card according to figure 4 and filed under the deposition number DSM 18890; d) DNA sequences coding for the protein sequence according to SEQ ID NO:2; e) DNA sequences which hybridize under stringent conditions with one of the DNA sequences listed under a), b), c) or d); f) DNA sequences which are related to the DNA/nucleotide sequences listed under a), b), c), d) or e) because of the degeneration of the genetic code; and g) complementary strands to the sequences listed under a) to f). The invention further relates to the protein coded thereby.
The invention relates to a method for recovering oil from plant seeds, characterized in that a) an aqueous solution, containing one or more cellulolytic, and/or lipolytic, and/or pectinolytic, and/or proteolytic enzyme or enzymes, and or phytases is sprayed onto the seed material; b) the seed material so obtained is supplied in a known manner to a one-step or multistep pressing which optionally is coupled to an extraction step; and c) the oil is recovered in a known manner and optionally processed further; and the use of the method, particularly for the production of food oil or biodiesel.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
CHEMICALS USED IN INDUSTRY, NAMELY, ENZYMES, ENZYME MIXTURES AND ENZYME PREPARATIONS FOR USE IN FRUIT JUICE PRODUCTION, FRUIT PROCESSING AND WINE-MAKING
47.
POLYPEPTIDE HAVING PHYTASE ACTIVITY AND INCREASED TEMPERATURE RESISTANCE OF THE ENZYME ACTIVITY, AND NUCLEOTIDE SEQUENCE CODING SAID POLYPEPTIDE
The invention relates to a recombinant DNA molecule coding a polypeptide having phytase activity after being expressed in a prokaryotic or eukaryotic host cell. Said recombinant DNA molecule comprises a DNA sequence selected from among a) DNA sequences coding a polypeptide that has phytase activity and is obtained by varying the mature wild-type E. coli phytase sequence, the variation being selected from among i) the mutation lysine → aspartic acid in position 74 (K74D), and/or ii) a combination of the mutations asparagine → arginine in position 139 (N139R) and aspartic acid → glutamic acid in position 142 (D142E), and/or iii) a combination of the mutations leucine → isoleucine in position 145 (L145I) and leucine → isoleucine in position 198 (L198I), and/or iv) a mutation valine → proline in position 200 (V200P), and/or v) an N-terminal or C-terminal or an N-terminal and C-terminal addition of a sequence section of the acidic phosphatase of Aspergillus niger var. awamori or the phytase of Aspergillus niger, b) DNA sequences that are 70 to 100 percent homologous to the sequences listed under a), c) DNA sequences which are related to the sequences listed under a) and b) because of the degeneration of the genetic code. When expressed in a suitable host cell, the recombinant DNA molecule has increased temperature resistance as well as increased protease stability of the enzyme activity of the protein coded in said manner. Variations iii) and iv) are provided only in combination with a variation i), ii), and v). The invention also relates to the proteins coded thereby.
The invention relates to a substance with increased thermal stability comprising a core and a coating layer, and characterised in that the core comprises at least one biologically active protein, and the coating layer comprises a micronised product from leguminous plants. Also disclosed are a method for producing this substance and the use of a micronised product from leguminous plants to increase the thermal stability of biologically active proteins during the production process of a granulated product, as well as a method for producing animal feed, foodstuffs and medicaments.
The invention relates to a DNA sequence which codes for a polypeptide having a lysophospholipase activity and isolated from Aspergillus, and sequences derived therefrom. The invention also relates to the polypeptides having a lysophospholipase activity and encoded by said sequences, and to the use of said polypeptides for improving the filterability of syrups consisting of wheat starch and for related uses.
The invention relates to a DNA sequence which codes for a polypeptide having a phospholipase activity and isolated from Aspergillus, and sequences derived therefrom. The invention also relates to the polypeptides having a lysophospholipase activity and encoded by said sequences, and to the use of said polypeptides for desliming plant oil, for producing pasta and/or pastries, for producing dairy products, for using in processing steps in the textile industry, and for related uses.
The invention relates to a vector construct for expressing and secreting polypeptides in filamentous fungi, comprising, in 5'3' direction, in functional linkage, - optionally a promoter which may have an enhancer placed upstream, at least one DNA sequence which codes for a first polypeptide and which may comprise a signal sequence or not, - optionally a DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, - a DNA sequence which codes for a second polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, - optionally a DNA sequence which codes for a third polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, optionally a DNA sequence which codes for a fourth polypeptide with signal sequence, and a terminator, where at least one 2A cleavage site or a sequence derived therefrom is present in the construct.
The present invention relates to a method for producing an improved enzyme containing product for use in the manufacture of animal feed. The present invention further provides a product obtainable by a method of the present invention, a method for preparing an animal feed comprising combining a product obtainable by a method of the present invention with suitable feed ingredients and an animal feed so produced.
The present invention relates to a method for producing an improved enzyme containing product for use in the manufacture of animal feed. The present invention further provides a product obtainable by a method of the present invention, a method for preparing an animal feed comprising combining a product obtainable by a method of the present invention with suitable feed ingredients and an animal feed so produced.
The invention relates to the use of an a-amylase obtainable from Thermoactinomyces vulgaris for producing dough and/or baked goods in which monoglyceride-based emulsifiers are partially or fully dispensed with, and to a method for producing baked goods which do not contain any or contain only small quantities of monoglyceride-based emulsifiers, characterised in that i) a dough for the particular baked good is produced using a method that is known per se but not using any or only small quantities of monoglyceride-based emulsifiers, ii) an a-amylase obtainable from Thermoactinomyces vulgaris is added to the dough or to part of the dough and iii) the dough is worked in a known manner to produce ready-to-eat baked goods.
The invention relates to a method for producing white pepper seeds, characterised in that a) ripe or substantially ripe pepper berries are washed with water one or more times, b) the washed pepper berries are treated with a preparation that contains pectinase(s), c) the fruit peel and fruit flesh of the pepper berries thus treated is mechanically removed, d) the pepper seeds thus obtained are washed with water one or more times and e) the washed pepper seeds are dried.
E. coli phytase, in particular, Quantum™ phytase. The invention further relates to hybridoma cell lines that produce anti-phytase monoclonal antibodies.
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
G01N 1/18 - Devices for withdrawing samples in the liquid or fluent state with provision for splitting samples into portions
59.
POLYPEPTIDE HAVING A PHYTASE ACTIVITY AND NUCLEOTIDE SEQUENCE CODING THEREFOR
The invention relates to a recombinant DNA molecule, which, upon expression in a prokaryotic or eukaryotic host cell, encodes a polypeptide having phytase activity, wherein the recombinant DNA molecule comprises a DNA sequence selected from a) DNA sequences that have been obtained by variations of the mature wild-type E. coli phytase sequence, wherein at least one amino acid in the region of position 189 to 211 and/or an amino acid in the region of position 137 to 152 is mutated as compared to the wild-type sequence, b) DNA sequences having a homology of 70% to 100% to the sequences according to a), c) DNA sequences that are related to the sequences according to a) and b) due to the degeneracy of the genetic code, wherein the recombinant DNA molecule is, upon expression in a suitable host cell, associated with an increased activity of the thus encoded protein in the culture supernatant, as well as the proteins encoded by the same.
01 - Chemical and biological materials for industrial, scientific and agricultural use
31 - Agricultural products; live animals
Goods & Services
Herbicide tolerant genes used for agricultural seeds and
chemical compounds for agriculture; enzyme for animal feed. Animal feed containing enzymes; additives to fodder (for
non-medical use).
01 - Chemical and biological materials for industrial, scientific and agricultural use
31 - Agricultural products; live animals
Goods & Services
Herbicide-tolerant genes for use in agricultural seed and chemical compounds for use in agriculture; enzyme for animal feed. Animal enzyme feed; fodder additives (not for medicinal purposes).
01 - Chemical and biological materials for industrial, scientific and agricultural use
31 - Agricultural products; live animals
Goods & Services
(1) Herbicide-tolerant genes for use in agricultural seed and chemical compounds for use in agriculture; enzyme for animal feed; fodder additives (not for medicinal purposes).
(2) Animal enzyme feed.
01 - Chemical and biological materials for industrial, scientific and agricultural use
29 - Meat, dairy products, prepared or preserved foods
30 - Basic staples, tea, coffee, baked goods and confectionery
32 - Beers; non-alcoholic beverages
Goods & Services
Produits chimiques pour l'industrie, les sciences et la
photographie, agents clarifiants, matières à clarifier des
jus de fruits; glucose, fécule. Gelées de fruits. Produits d'amidon pour aliments et denrées alimentaires,
glucose, fécule. Eaux minérales, boissons non alcooliques, jus de fruits,
sirop de fécule.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzyme Agents for Improving the Baking Qualities of Flour Used as Additives in the Preparation of Flour, in the Preparation of Leavening Agents and as Baking-Additive in the Food and Baking Industries
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
(1) Enzyme preparations for starch processing, enzyme preparations for fruit processing, enzyme preparations for use in the beverage industry, starch-splitting enzymes, sugar-splitting enzymes, all for use in industry and science.
01 - Chemical and biological materials for industrial, scientific and agricultural use
02 - Paints, varnishes, lacquers
03 - Cosmetics and toiletries; cleaning, bleaching, polishing and abrasive preparations
Goods & Services
Produits chimiques pour l'industrie, les sciences et la
photographie, amidon et fécule pour la technique, les
sciences et la photographie, agents émulsionnants, à doubler
des tissus, liants et de dispersion pour la technique,
glycérine (non pour des buts médicinaux), matières de
remplissage pour matériaux plastiques, matières de
remplissage pour l'industrie du cuir et du savon, solvants
pour laques, dissolvants des résines, produits extincteurs,
trempes, soudures, esters de résines, matières à dégraisser
le cuir, compositions de résines synthétiques, matières
plastiques contenant des résines synthétiques, compositions
de résines synthétiques chargées de fibres textiles et
fibreuses, bois artificiel plastiques, matières premières
minérales (comprises dans la classe 1), matières à améliorer
des laques, matières premières pour laques, résines, résines
synthétiques, glutens pour des buts industriels, colles,
pellicules collantes, dextrine, amidon apprêtant et amidon
gonflant, agents d'encollage, matières à apprêter et à
tanner, produits de pelanage. Agents colorants pour l'industrie du cuir, du textile et du
papier, vernis, laques, matières à améliorer des laques,
matières premières pour laques, siccatifs, mordants,
résines, agents liants et épaississants pour couleurs,
matières pour le traitement du plancher. Cirages, matières à astiquer le cuir, matières à polir des
bois, cuir, papier et pierres; matières à polir des bois,
cuir, papier et pierres; matières pour le traitement du
plancher, cire à parquet.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes for Starch Processing, Enzymes for Fruit Processing, Enzymes for Use in the Production of Alcohol and Beer, Starch-Splitting Enzymes, Sugar-Splitting Enzymes, All for Use in Industry and Science
01 - Chemical and biological materials for industrial, scientific and agricultural use
02 - Paints, varnishes, lacquers
03 - Cosmetics and toiletries; cleaning, bleaching, polishing and abrasive preparations
Goods & Services
Produits chimiques pour l'industrie, les sciences et la
photographie, amidon et fécule pour la technique, les
sciences et la photographie, agents émulsionnants, à doubler
des tissus et agents liants et de dispersion pour la
technique, matières de remplissage pour matériaux
plastiques, matières de remplissage pour l'industrie du cuir
et du savon, solvants pour laques, dissolvants des résines,
produits extincteurs, trempes, soudures, esters de résines,
matières à dégraisser le cuir, compositions des résines
synthétiques, matières plastiques contenant des résines
synthétiques, matières plastiques chargées de fibres
textiles et de matières fibreuses, résines synthétiques;
matières premières pour laques, résines, glutens et colles
pour l'industrie, dextrine, agents d'encollage; matières à
apprêter et à tanner, produits de pelanage. Vernis, laques, matières à améliorer des laques, matières
premières pour laques, siccatifs, mordants, résines, agents
liants et épaississants pour couleurs, agents colorants pour
l'industrie du cuir, du textile et du papier. Préparations à enlever des laques, matières à dégraisser le
cuir.
01 - Chemical and biological materials for industrial, scientific and agricultural use
29 - Meat, dairy products, prepared or preserved foods
30 - Basic staples, tea, coffee, baked goods and confectionery
31 - Agricultural products; live animals
32 - Beers; non-alcoholic beverages
Goods & Services
Auxiliary agents for cooking, namely diastase malt-based
preparations, enzyme preparations of animal, vegetable and
bacterial origin, acidifying agents for dough, mixtures of
the above-mentioned auxiliary cooking agents, also involving
the use of inorganic materials for food purposes; chemical
products for use in industry (except auxiliary agents and
semi-finished products for the manufacture of cosmetic,
pharmaceutical and disinfectant products, particularly
chemical products for the milling industry, starch and
starch products for technical applications, for use in
science and photography, flour-improving preparations,
enzyme preparations of animal, vegetable and bacterial
origin for use as addition agents for flour; agents for
clarifying fruit juices; farina. Vegetable juices, fruit and vegetable jellies. Yeasts, baking powders, raising flours, mixtures of raising
flours and agents for acidifying dough; starch decomposing
agents for particularly cooking maltose, mixtures of the
above-mentioned auxiliary cooking agents, also involving the
use of inorganic materials for food purposes;
flour-improving preparations; starch preparations for food
and consumption products, starch sugar, starch syrup,
starch. Auxiliary agents for cooking, particularly malt for cooking. Fruit and vegetable juices.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Produits chimiques destinés à l'industrie, la science et la
photographie; agents auxiliaires chimiques et produits
chimiques semi-finis pour l'industrie des aliments et des
boissons, ainsi que pour la fabrication de produits
cosmétiques; matières premières pour la fabrication de
préparations pour lessiver, matières à améliorer les terres;
enzymes, compositions d'enzymes et produits chimiques
contenant des enzymes pour buts chimico-scientifiques et
techniques; mouillants, produits chimiques pour supprimer la
mousse, produits à dégraisser le cuir, confits pour
tanneries, matériaux tannants, matières à tanner et agents
auxiliaires pour le tannage, produits d'enchaussenage et de
pelanage, dépilatoires et produits pour le délainage,
matières de trempe pour les cuirs et les peaux; matières à
améliorer les textiles, matières à apprêter, agents
d'encollage et de désencollage.
