A method for introducing foreign nucleic acids into cells assisted with acoustophoresis and including a step of continuously sweeping a frequency of acoustic waves from a first frequency f1 to a second frequency f2 using a sweep time ranging from 1 ms to 100 ms, f2 being superior to f1. Also, a method for performing transduction of cells, cells obtained by the method and an acoustophoresis device for introducing foreign nucleic acids into cells including a chamber, at least two inlets, at least two outlets and at least one acoustic wave generator is configured to continuously sweep a frequency of the acoustic waves from a first frequency f1 to a second frequency f2 using a sweep time ranging from 1 ms to 100 ms, f2 being superior to f1.
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
2.
DEVICE FOR PARTICLES HANDLING, WASHING, TRANSFECTION THROUGH ACOUSTOPHORETIC INDUCED MIGRATION
A device for separating and/or isolating and/or washing target particles from a particles suspension. The device includes at least two inlets, at least two outlets, a container having a longitudinal axis and a chamber for fluid flow, being configured to be associated with a transducer, at least one transducer configured to generate bulk acoustic waves within the chamber, and at least one flow rate sensor configured to measure the flow rate of the fluid in the chamber. The inlets are located on one end of the container and the outlets are located on the other end along the longitudinal axis. The first and second inlet are each located on either side of the longitudinal axis, the first inlet and the second outlet are each located on either side of the longitudinal axis, and the second inlet and the first outlet are each located on either side of the longitudinal axis.
A system for changing a concentration of at least one group of particles in a fluid, which includes a first container and a second container; a first transfer device and a second transfer device, and an element for keeping the volume of fluid in the first and second containers constant. The first container is fluidly connected to an inlet of the first transfer device, and the second container is fluidly connected to an inlet of the second transfer device. The first and second transfer devices include a chamber associated with at least one acoustic wave generator for generating acoustic waves within the chamber; at least two outlets including a first outlet for fluid enriched with the particles and a second outlet for fluid depleted of the particles; the first outlet being fluidly connected to the first container and the second outlet being fluidly connected to the second container.
INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) (France)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (France)
ÉCOLE SUPÉRIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS (France)
Inventor
Provost, Jean
Gachelin, Jérémie
Pialot, Baptiste
Couture, Olivier
Vincent, Emmanuel
Abstract
A method for determining a flow rate and/or a concentration of particles of a fluid flowing in a chamber, which includes the steps of: producing an ultrasound beam of a given frequency with a first transducer such that all fluid components traveling through an intersection region between the ultrasound beam and the chamber are insonated by the first transducer; receiving Doppler-shifted ultrasound signals generated by the fluid components in the insonated region of the chamber with a second transducer; acquiring the ultrasound signals received by the second transducer during an acquisition time; obtaining a Doppler Power Spectrum of the acquired ultrasound signals; and determining the flow rate and/or the concentration of particles of the fluid by adjustment between, on the one hand, the obtained Doppler Power Spectrum and, on the other hand, a model of the Doppler Power Spectrum.
G01F 1/06 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by using mechanical effects using rotating vanes with tangential admission
G01F 1/663 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters by measuring Doppler frequency shift
G01N 15/06 - Investigating concentration of particle suspensions
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
6.
DEVICE FOR PARTICLES HANDLING, WASHING, TRANSFECTION THROUGH ACOUSTOPHORETIC INDUCED MIGRATION
The present invention relates to a device for separating and/or isolating and/or washing target particles from a particles suspension, said device comprising: - at least two inlets (113, 114); - at least two outlets (115, 116); - a container having a longitudinal axis (A1), comprising a chamber (111) for fluid flow, said chamber (111) being configured to be associated with a transducer (112); - at least one transducer (112) configured to generate bulk acoustic waves within the chamber (111); - at least one flow rate sensor configured to measure the flow rate of the fluid in the chamber (111); wherein at least two inlets (113, 114) are located on one end of the container and at least two outlets (115, 116) are located on the other end along longitudinal axis (A1) of the container; wherein the at least one first inlet and the second inlet are each located on either side of longitudinal axis (A1) of the container, wherein the at least one first inlet and the second outlet are each located on either side of longitudinal axis (A1) of the container, and wherein the second inlet and the at least one first outlet are each located on either side of longitudinal axis (A1) of the container.
INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) (France)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (France)
ÉCOLE SUPÉRIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS (France)
Inventor
Provost, Jean
Gachelin, Jérémie
Pialot, Baptiste
Couture, Olivier
Abstract
The present invention relates to a method for determining a flow rate and/or a concentration of particles of a fluid flowing in a chamber (2), the method comprising steps of: - producing an ultrasound beam of a given frequency by means of a first transducer (61), in such a way that all fluid components traveling through an intersection region between the ultrasound beam and the chamber (2) are insonated by the first transducer (61); - receiving Doppler-shifted ultrasound signals generated by the fluid components in said insonated region of the chamber (2) by means of a second transducer (62); - acquiring the ultrasound signals received by the second transducer (62) during an acquisition time; - obtaining a Doppler Power Spectrum of the acquired ultrasound signals; - determining the flow rate and/or the concentration of particles of the fluid by adjustment between, on the one hand, the obtained Doppler Power Spectrum and, on the other hand, a model of the Doppler Power Spectrum.
G01F 1/66 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters
This system (1) for changing a concentration of at least one given group of particles comprised in a fluid, the system comprising: - a first container (11) and a second container (12); - a first transfer device (13) and a second transfer device (14), wherein the first container (11) is fluidly connected to an inlet (133) of the first transfer device (13) and the second container (12) is fluidly connected to an inlet (143) of the second transfer device (14), each of the first and second transfer devices (13, 14) comprising: a chamber configured to be associated with at least one acoustic wave generator for generating acoustic waves within the chamber; at least two outlets (134, 135, 144, 145) comprising a first outlet (134, 144) for fluid enriched with said given group of particles and a second outlet (135, 145) for fluid depleted of said given group of particles; the first outlet (134, 144) being fluidly connected to the first container (11) and the second outlet (135, 145) being fluidly connected to the second container (12); and - means for keeping the volume of fluid in each of the first and second containers (11, 12) constant.
Disclosed is a multiple bag system for fractionating blood, the system including a fluid collecting bag including at least one outlet port; at least first and second sampling bags, each including at least one inlet port and at least one outlet port; and a unit for transferring fluid from the fluid collecting bag to the sampling bags; wherein the unit for transferring fluid includes an acoustic sorter. Also disclosed is a method for fractionating blood into blood products.
Disclosed is a multiple bag system for fractionating a fluid, including a fluid collecting bag with at least one outlet port; at least first and second sampling bags, each having at least one inlet port and at least one outlet port; and a mechanism for transferring fluid from the fluid collecting bag to the sampling bags. The fluid transfer mechanism includes an acoustic sorter. Also disclosed is a method for fractionating a fluid into fluid products.
A61L 33/00 - Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of bloodMaterials for such treatment
A61M 1/36 - Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
B01D 21/28 - Mechanical auxiliary equipment for acceleration of sedimentation, e.g. by vibrators or the like
G01N 15/02 - Investigating particle size or size distribution
Disclosed is a device, suitable for use as an acoustic resonator, including a base adapted to be coupled to at least one acoustic wave generator, a spacer including an aperture and a reflector, wherein the base includes a protruding part having a thickness t; the aperture of the spacer is complementary to the protruding part of the base; the device further includes a housing having an aperture complementary to the protruding part of the base and wherein the inner edge of the aperture has the same thickness t than the protruding part; and the housing is positioned between the spacer and the reflector, such that the thickness of the inner edge of the spacer defined the thickness of a cavity between the protruding part and the reflector. Also disclosed is a method of trapping particles in a fluid.
Disclosed is a multiple bag system for fractionating blood, the system including a fluid collecting bag including at least one outlet port; at least first and second sampling bags, each including at least one inlet port and at least one outlet port; and a fluid transfer unit to transfer fluid from the collecting bag to the sampling bags. The fluid transfer unit includes an acoustic sorter. Also disclosed is a method for fractionating blood into blood products.
The present invention relates to a multiple bag system for fractionating blood, said system comprising a fluid collecting bag (B.00) comprising at least one outlet port; at least first and second sampling bags (B.10, B.12), each comprising at least one inlet port and at least one outlet port; and means for transferring fluid from the fluid collecting bag (B.00) to the sampling bags (B.10, B.12); wherein the means for transferring fluid comprises an acoustic sorter (AS0). The present invention also relates to a method for fractionating blood into blood products.
The present invention relates to a multiple bag system for fractionating blood, said system comprising a fluid collecting bag (B.00) comprising at least one outlet port; at least first and second sampling bags (B.10, B.12), each comprising at least one inlet port and at least one outlet port; and means for transferring fluid from the fluid collecting bag (B.00) to the sampling bags (B.10, B.12); wherein the means for transferring fluid comprises an acoustic sorter (AS0). The present invention also relates to a method for fractionating blood into blood products.
The present invention relates to a device (1), suitable for use as an acoustic resonator, comprising a base (2) adapted to be coupled to at least one acoustic wave generator, a spacer (3) comprising an aperture (31) and a reflector (5), wherein the base (2) comprises a protruding part (21) having a thickness t; the aperture of the spacer (31) is complementary to the protruding part of the base (21); the device (1) further comprises a housing (4) having an aperture (41) complementary to the protruding part of the base (21) and wherein the inner edge of the aperture (41) has the same thickness t than the protruding part (21); and the housing (4) is positioned between the spacer (3) and the reflector (5), such that the thickness of the inner edge of the spacer (3) defined the thickness of a cavity between the protruding part (21) and the reflector (5). The present invention also relates to a method of trapping particles in a fluid.
The present invention relates to a device (1), suitable for use as an acoustic resonator, comprising a base (2) adapted to be coupled to at least one acoustic wave generator, a spacer (3) comprising an aperture (31) and a reflector (5), wherein the base (2) comprises a protruding part (21) having a thickness t; the aperture of the spacer (31) is complementary to the protruding part of the base (21); the device (1) further comprises a housing (4) having an aperture (41) complementary to the protruding part of the base (21) and wherein the inner edge of the aperture (41) has the same thickness t than the protruding part (21); and the housing (4) is positioned between the spacer (3) and the reflector (5), such that the thickness of the inner edge of the spacer (3) defined the thickness of a cavity between the protruding part (21) and the reflector (5). The present invention also relates to a method of trapping particles in a fluid.
The present invention relates to a multiple bag system for fractionating blood, said system comprising a fluid collecting bag (B.00) comprising at least one outlet port; at least first and second sampling bags (B.10, B.12), each comprising at least one inlet port and at least one outlet port; and means for transferring fluid from the fluid collecting bag (B.00) to the sampling bags (B.10, B.12); wherein the means for transferring fluid comprises an acoustic sorter (AS0). The present invention also relates to a method for fractionating blood into blood products.
The present invention relates to a multiple bag system for fractionating blood, said system comprising a fluid collecting bag (B.00) comprising at least one outlet port; at least first and second sampling bags (B.10, B.12), each comprising at least one inlet port and at least one outlet port; and means for transferring fluid from the fluid collecting bag (B.00) to the sampling bags (B.10, B.12); wherein the means for transferring fluid comprises an acoustic sorter (AS0). The present invention also relates to a method for fractionating blood into blood products.
patents
