The present invention relates to a mutated hydroxyphenylpyruvate dioxygenase (HPPD) polypeptide, and a coding gene and use thereof. The mutated HPPD polypeptide retains the activity of catalyzing the conversion of 4-hydroxyphenylpyruvic acid into homogentisic acid or homogentisate, and has lower sensitive to an HPPD inhibitor herbicide than that to wild type HPPD. An amino acid sequence corresponding to the amino acid sequence as shown in SEQ ID NO: 1 comprises amino acid mutation at the following sites: an F372 site is substituted by A, G or V, and an F383 site is substituted by W. The present invention discloses for the first time that the combination mutation at the 372 site and the 383 site of HPPD polypeptides from different species sources can endow plants with synergistic tolerance to HPPD inhibitor herbicides, and the application prospect in plants is wide.
The present invention relates to a nucleic acid sequence for detecting corn plant DBN9229 and a detection method therefor, wherein the nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, or SEQ ID NO: 2 or a complementary sequence thereof. The corn plant DBN9229 has better resistance to Lepidopteran and better tolerance to glufosinate herbicides and has no influence on the yield, and the detection method can accurately and quickly identify whether a DNA molecule of the transgenic corn event DBN9229 is contained in a biological sample.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
The present invention relates to a nucleic acid sequence for detecting corn plant DBN9235 and a detection method therefor, wherein the nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, or SEQ ID NO: 2 or a complementary sequence thereof. The corn plant DBN9235 has better resistance to Lepidopteran and better tolerance to glufosinate herbicides and has no influence on the yield, and the detection method can accurately and quickly identify whether a DNA molecule of the transgenic corn event DBN9235 is contained in a biological sample.
The present invention relates to the use of a protoporphyrinogen oxidase. A method for controlling weeds comprises applying a herbicide containing an effective dose of a PPO inhibitor to a field in which at least one transgenic plant is present, wherein the transgenic plant comprises, in the genome thereof, a polynucleotide sequence encoding protoporphyrinogen oxidase, and the transgenic plant has reduced plant damage and/or has an increased plant yield compared with other plants that do not have a polynucleotide sequence encoding protoporphyrinogen oxidase. The protoporphyrinogen oxidase PPO1-PPO14 of the present invention has a high tolerance to a PPO inhibitor herbicide. In addition, the plants containing the polynucleotide sequence encoding protoporphyrinogen oxidase have a strong tolerance to the PPO-inhibitor herbicide, and show high-resistance tolerance to almost all of 4-fold field concentration of oxyfluorfen, saflufenacil and flumioxazin and 2-fold field concentration of sulfentrazone. Therefore, the protoporphyrinogen oxidase has broad application prospects in plants.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
A01N 33/22 - Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
A01N 43/84 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms, as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
A use of an insecticidal protein. The insecticidal protein can be used for controlling the pest Etiella zinckenella. A method for controlling the pest Etiella zinckenella comprises: making the pest Etiella zinckenella at least in contact with the Cry1Ac protein. The pest Etiella zinckenella is controlled by generating the Cry1Ac protein capable of killing Etiella zinckenella in a plant body. Compared with an agricultural prevention and treatment method, a chemical prevention and treatment method and a biological prevention and treatment method used in the prior art, the method achieves protection of the whole plant during the whole growth period so as to prevent and treat invasion of the pest Etiella zinckenella, the method does not cause pollution or residue, and the method has a stable and thorough effect, and is simple, convenient and economical.
Use of an insecticidal protein. The insecticidal protein can be used for controlling Etiella zinckenella pests. The method for controlling the Etiella zinckenella pests comprises: allowing the Etiella zinckenella pests to be at least in contact with a Vip3Aa protein. The method controls the Etiella zinckenella pests by means of the Vip3Aa protein that is produced in a plant and capable of killing the Etiella zinckenella pests. Compared with the agricultural control methods, chemical control methods, and biological control methods used in the prior art, the method provides the whole plant with protection throughout the growth period so as to prevent and control the infestation of the Etiella zinckenella pests, and is pollution-free, residue-free, stable and thorough in effect, simple, convenient, and economical.
The present invention relates to use of protoporphyrinogen oxidase. A method for controlling weeds comprises applying a herbicide containing an effective dose of PPO inhibitor to a field with at least one transgenic plant. The transgenic plant has a polynucleotide sequence encoding protoporphyrinogen oxidase in a genome thereof, and has reduced plant damage and/or increased plant production compared to other plants that do not have a polynucleotide sequence encoding protoporphyrinogen oxidase. According to the present invention, the protoporphyrinogen oxidase PPOA-POF has a relatively high tolerance to a PPO inhibitor herbicide, and the plants having the polynucleotide sequence encoding protoporphyrinogen oxidase have great tolerance to the PPO inhibitor herbicide, and show high-resistance tolerance to almost all 4-fold field concentrations of saflufenacil, oxyfluorfen, and flumioxazin. Therefore, the application prospect for plants is wide.
Provided are a nucleic acid sequence for detecting a Glycine max plant DBN8205 and a detection method therefor. The nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and/or SEQ ID NO: 2 or a complementary sequence thereof. The Glycine max plant DBN8205 has good resistance to insects of Lepidoptera order and good tolerance to glufosinate herbicides, and therefore the yield is not affected. By utilizing the detection method, it is possible to accurately and quickly identify whether a biological sample contains a DNA molecule of a transgenic Glycine max event DBN8205.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
A23J 1/14 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seedsObtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from press-cake or oil-bearing seeds
A23D 9/00 - Other edible oils or fats, e.g. shortenings or cooking oils
A23L 29/30 - Foods or foodstuffs containing additivesPreparation or treatment thereof containing carbohydrate syrupsFoods or foodstuffs containing additivesPreparation or treatment thereof containing sugarsFoods or foodstuffs containing additivesPreparation or treatment thereof containing sugar alcohols, e.g. xylitolFoods or foodstuffs containing additivesPreparation or treatment thereof containing starch hydrolysates, e.g. dextrin
A23L 29/00 - Foods or foodstuffs containing additivesPreparation or treatment thereof
Related is a use of an insecticidal protein. The insecticidal protein may be used to control a thrip pest. A method for controlling the a thrip pest includes: allowing the a thrip pest to be at least in contact with an ACh1 protein. In the present application, the a thrip pest is controlled through producing the ACh1 protein that can kill the a thrip pest in bacteria and/or a plant in vivo.
Related is a use of an insecticidal protein. The insecticidal protein may be used to control Monolepta hieroglyphica (Motschulsky). A method for controlling the Monolepta hieroglyphica (Motschulsky) includes: allowing the Monolepta hieroglyphica (Motschulsky) to be at least in contact with an ACe1 protein. In the present application, the ACe1 protein that can kill the Monolepta hieroglyphica (Motschulsky) is produced in bacteria and/or a plant body to control the Monolepta hieroglyphica (Motschulsky).
Lepidoptera insects as well as good tolerance to glufosinate herbicide without compromising the yield, and the detection methods can accurately and rapidly identify whether a biological sample contains the DNA molecule of the transgenic soybean event DBN8002.
Related is a use of an insecticidal protein. The insecticidal protein may be used to control Monolepta hieroglyphica (Motschulsky). A method for controlling the Monolepta hieroglyphica (Motschulsky) includes: allowing the Monolepta hieroglyphica (Motschulsky) to be at least in contact with an ACh1 protein. In the present application, the Monolepta hieroglyphica (Motschulsky) is controlled through producing the ACh1 protein that can kill the Monolepta hieroglyphica (Motschulsky) in bacteria and/or a plant in vivo.
Provided is a mutant hydroxyphenylpyruvate dioxygenase (HPPD) polypeptide, an encoding gene thereof and a use thereof. The mutant HPPD polypeptide retains the activity of catalyzing the conversion of hydroxyphenylpyruvate acid into homogentisic acid or homogentisate, and the sensitivity to HPPD inhibitor herbicides is lower than that of original unmutated HPPD. On position 372 corresponding to the amino acid sequence represented by SEQ ID NO: 1, the mutant HPPD polypeptide comprises the following mutations: F372A, F372G, F372V, F372P, F372S, F372T, F372C, F372M, F372Q, F372D or F372 deletion. The described mutant can provide plants with high tolerance to HPPD inhibitor herbicides, and can be used to cultivate plants that are tolerant to HPPD inhibitor herbicides.
The present invention relates to a mutated hydroxyphenylpyruvate dioxygenase (HPPD) polypeptide, and a coding gene and use thereof. The mutated HPPD polypeptide retains the activity of catalyzing the conversion of 4-hydroxyphenylpyruvic acid into homogentisic acid or homogentisate, and has lower sensitivity to an HPPD inhibitor herbicide than that to wild type HPPD. An amino acid sequence corresponding to the amino acid sequence as shown in SEQ ID NO: 1 comprises amino acid mutation at the following sites: an F372 site is substituted by A, G or V, and an F383 site is substituted by W. The present invention discloses for the first time that the combination mutation of the 372 site and the 383 site of HPPD polypeptides from different species sources can endow plants with synergistic tolerance to HPPD inhibitor herbicides, and the application prospect in plants is wide.
The present invention relates to a mutated hydroxyphenylpyruvate dioxygenase (HPPD) polypeptide, and a coding gene and use thereof. The mutated HPPD polypeptide retains the activity of catalyzing the conversion of 4-hydroxyphenylpyruvic acid into homogentisic acid or homogentisate, and has lower sensitivity to an HPPD inhibitor herbicide than that to wild type HPPD. An amino acid sequence corresponding to the amino acid sequence as shown in SEQ ID NO: 1 comprises amino acid mutation at the following sites: an F372 site is substituted by A, G or V, and an F383 site is substituted by W. The present invention discloses for the first time that the combination mutation of the 372 site and the 383 site of HPPD polypeptides from different species sources can endow plants with synergistic tolerance to HPPD inhibitor herbicides, and the application prospect in plants is wide.
Related is a use of an insecticidal protein. The insecticidal protein may be used to control Apolygus lucorum. A method for controlling the Apolygus lucorum includes: allowing the Apolygus lucorum to be at least in contact with an ACh1 protein. In the present application, the ACh1 protein that can kill the Apolygus lucorum is produced in bacteria and/or a plant in vivo to control the Apolygus lucorum.
Related is a use of an insecticidal protein. The insecticidal protein may be used to control Ostrinia furnacalis (Hubern). A method for controlling Ostrinia furnacalis (Hubern) includes: allowing the Ostrinia furnacalis (Hubern) to be at least in contact with an ACe1 protein. In the present application, the ACe1 protein that can kill the Ostrinia furnacalis (Hubern) is produced in bacteria and/or a plant body to control the Ostrinia furnacalis (Hubern).
A01N 61/00 - Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
The present invention relates to the use of a protoporphyrinogen oxidase. A method for controlling weeds comprises applying a herbicide containing an effective dose of a PPO inhibitor to a field in which at least one transgenic plant is present, wherein the transgenic plant comprises, in the genome thereof, a polynucleotide sequence encoding protoporphyrinogen oxidase, and the transgenic plant has reduced plant damage and/or has an increased plant yield compared with other plants that do not have a polynucleotide sequence encoding protoporphyrinogen oxidase. The protoporphyrinogen oxidase PPO1-PPO14 of the present invention has a high tolerance to a PPO inhibitor herbicide. In addition, the plants containing the polynucleotide sequence encoding protoporphyrinogen oxidase have a strong tolerance to the PPO inhibitor herbicide, and show high-resistance tolerance to almost all of 4-fold field concentration of oxyfluorfen, saflufenacil and flumioxazin and 2-fold field concentration of sulfentrazone. Therefore, the protoporphyrinogen oxidase has broad application prospects in plants.
The present invention relates to the use of a protoporphyrinogen oxidase. A method for controlling weeds comprises applying a herbicide containing an effective dose of a PPO inhibitor to a field in which at least one transgenic plant is present, wherein the transgenic plant comprises, in the genome thereof, a polynucleotide sequence encoding protoporphyrinogen oxidase, and the transgenic plant has reduced plant damage and/or has an increased plant yield compared with other plants that do not have a polynucleotide sequence encoding protoporphyrinogen oxidase. The protoporphyrinogen oxidase PPO1-PPO14 of the present invention has a high tolerance to a PPO inhibitor herbicide. In addition, the plants containing the polynucleotide sequence encoding protoporphyrinogen oxidase have a strong tolerance to the PPO inhibitor herbicide, and show high-resistance tolerance to almost all of 4-fold field concentration of oxyfluorfen, saflufenacil and flumioxazin and 2-fold field concentration of sulfentrazone. Therefore, the protoporphyrinogen oxidase has broad application prospects in plants.
The present invention relates to an herbicide tolerance protein, an encoding gene thereof and use thereof, the herbicide tolerance protein comprising: a protein (a) having an amino acid sequence as shown in SEQ ID NO: 1, and having an alanine substitution at least at position 176 and/or having a valine substitution at position 178 of SEQ ID NO: 1; or (b) having an amino acid sequence as shown in SEQ ID NO: 3; or (c) having an amino acid sequence as shown in SEQ ID NO: 5; or (d) having an amino acid sequence as shown in SEQ ID NO: 7; or (e) being derived from (a) by means of the amino acid sequence of (a) undergoing substitution and/or deletion and/or by added one or several amino acids, and having the activity of thifensulfuron hydrolase. The herbicide tolerance protein of the present invention has a broad application prospects in plants.
A01N 47/36 - Ureas or thioureas containing the groups N—CO—N or N—CS—N containing the group N—CO—N directly attached to at least one heterocyclic ringThio-analogues thereof
21.
Method for improving soybean transformation efficiency
The present invention relates to a method of improving soybean transformation efficiency, which comprises: transforming a plant cell using a recombinant vector containing a gene of interest and a gene encoding a sulfonylurea herbicide hydrolase; screening and culturing the transformed plant cell by external application of an ALS inhibitor, using the gene encoding the sulfonylurea herbicide hydrolase as a selective marker; selecting a plant cell that has not been killed and/or not been inhibited. The present invention firstly proposes that a selective agent is added to a proliferation medium and a differentiation medium in a manner of external application during plant transformation process, and optimizes the effective screening concentration range of the selective agent, so the transformation efficiency is remarkably increased, and the proportion of positive plants obtained in the progeny thereof is significantly increased; at the same time, the transgenic plants obtained by the transformation using the sulfonylurea herbicide hydrolase gene as a selective marker in the present invention have high commercial value, good resistance and genetic stability.
Monolepta hieroglyphica, comprising: a) a polynucleotide sequence shown as SEQ ID NO: 1; or (b) a polynucleotide sequence having at least 15 or 17 or 19 or 21 contiguous nucleotides of SEQ ID NO: 1, a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence being capable of inhibiting the growth of coleopteran pests after being ingested by the coleopteran pests; or (c) any one of polynucleotide sequences shown as SEQ ID NO: 3 to SEQ ID NO: 20; or (d) a polynucleotide sequence that hybridizes under stringent conditions to a polynucleotide sequence as defined in (a), (b) or (c).
A01N 25/02 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
24.
MUTANT HYDROXYPHENYLPYRUVATE DIOXYGENASE POLYPEPTIDE, ENCODING GENE THEREOF AND USE THEREOF
Provided is a mutant hydroxyphenylpyruvate dioxygenase (HPPD) polypeptide, an encoding gene thereof and a use thereof. The mutant HPPD polypeptide retains the activity of catalyzing the conversion of hydroxyphenylpyruvate acid into homogentisic acid or homogentisate, and the sensitivity to HPPD inhibitor herbicides is lower than that of original unmutated HPPD. On position 372 corresponding to the amino acid sequence represented by SEQ ID NO: 1, the mutant HPPD polypeptide comprises the following mutations: F372A, F372G, F372V, F372P, F372S, F372T, F372C, F372M, F372Q, F372D or F372 deletion. The described mutant can provide plants with high tolerance to HPPD inhibitor herbicides, and can be used to cultivate plants that are tolerant to HPPD inhibitor herbicides.
Provided is a mutant hydroxyphenylpyruvate dioxygenase (HPPD) polypeptide, an encoding gene thereof and a use thereof. The mutant HPPD polypeptide retains the activity of catalyzing the conversion of hydroxyphenylpyruvate acid into homogentisic acid or homogentisate, and the sensitivity to HPPD inhibitor herbicides is lower than that of original unmutated HPPD. On position 372 corresponding to the amino acid sequence represented by SEQ ID NO: 1, the mutant HPPD polypeptide comprises the following mutations: F372A, F372G, F372V, F372P, F372S, F372T, F372C, F372M, F372Q, F372D or F372 deletion. The described mutant can provide plants with high tolerance to HPPD inhibitor herbicides, and can be used to cultivate plants that are tolerant to HPPD inhibitor herbicides.
A use of an insecticidal protein, comprising: bringing a Helicoverpa gelotopoeon pest into contact with at least a Vip3Aa protein; and controlling the Helicoverpa gelotopoeon pest by means of producing in a plant a Vip3Aa protein capable of killing the Helicoverpa gelotopoeon.
Disclosed are a nucleic acid sequence for detecting soybean plant DBN8002 and a detection method therefor. The nucleic acid sequence comprises SEQ ID NO: 1 or the complementary sequence thereof, and/or SEQ ID NO: 2 or the complementary sequence thereof. The soybean plant DBN8002 has a relatively good resistance to Lepidoptera insects, has a relatively good tolerance of glufosinate-ammonium herbicides, and has no influence on the yield, and the detection method can accurately and rapidly identify whether a biological sample contains a DNA molecule of the transgenic soybean event DBN8002.
A nucleic acid sequence used for detecting a soybean plant DBN8007 and a detection method therefor, the nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and/or SEQ ID NO: 2 or a complementary sequence thereof. The soybean plant DBN8007 has good resistance to lepidopteran insects and has good tolerance of glufosinate-ammonium herbicide, which has no effect on the yield. In addition, the detection method may accurately and quickly identify whether a biological sample contains a DNA molecule of transgenic soybean event DBN8007.
Provided are a nucleic acid sequence for detecting a genetically modified maize event DBN9501 and a detection method therefor, the nucleic acid sequence comprising SEQ ID NO: 1 or a complementary sequence thereof, or SEQ ID NO: 2 or a complementary sequence thereof. Also provided are a method for preparing a maize plant that is resistant to insects and/or tolerant to glufosinate-ammonium herbicide, a method for cultivating a maize plant that is resistant to insects and/or tolerant to glufosinate-ammonium herbicide, and a method for protecting a maize plant from damage caused by herbicides or for controlling weeds in the field in which a maize plant is grown, the genome of the maize plant comprising nucleic acid sequences shown in SEQ ID NO: 1 and/or SEQ ID NO:2, nucleic acid sequences shown in SEQ ID NO:3 and/or SEQ ID NO:4 or a nucleic acid sequence shown in SEQ ID NO:5.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
30.
POLYNUCLEOTIDE AND METHOD USED FOR CONTROLLING INSECT INVASION
Disclosed by the present invention are an isolated polynucleotide and method used for controlling insect invasion, the isolated polynucleotide comprising: (a) a polynucleotide sequence as shown in SEQ ID NO: 1; or (b) a polynucleotide sequence having at least 15, 17, 19 or 21 contiguous nucleotides of SEQ ID NO: 1, wherein the growth of a pest of the order Coleoptera is inhibited when the pest of the order Coleoptera ingests double-stranded RNA comprising at least one strand that is complementary to the described polynucleotide sequence; or (c) any polynucleotide sequence as shown in SEQ ID NO: 3 to SEQ ID NO: 6; or (d) a polynucleotide sequence which hybridizes under stringent conditions with the polynucleotide sequence defined in (a), (b) or (c). Disclosed by the present invention for the first time is a plurality of target sequences used for controlling a target gene c4506 of Monolepta hieroglyphica, which is a pest of the order Coleoptera.
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01N 57/16 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
Disclosed are an isolated polynucleotide and a method for controlling insect infestation, wherein the isolated polynucleotide comprises: (a) a polynucleotide sequence as shown in SEQ ID NO: 1; or (b) a polynucleotide sequence comprising at least 15 or 17 or 19 or 21 consecutive nucleotides of SEQ ID NO: 1, wherein the ingestion, by Coleoptera insect pests, of a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence inhibits the growth of the Coleoptera insect pests; or (c) any one of the polynucleotide sequences as shown in SEQ ID NO: 3 to SEQ ID NO: 6; or (d) a polynucleotide sequence hybridized with the polynucleotide sequence defined in (a), (b) or (c) mentioned above under stringent conditions. The present invention discloses multiple target sequences of the target gene c46312 for controlling the Coleoptera insect pest Monolepta hieroglyphica for the first time.
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01N 57/16 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
Disclosed are an isolated polynucleotide and a method for controlling insect infestation, wherein the isolated polynucleotide comprises: (a) a polynucleotide sequence as shown in SEQ ID NO: 1; or (b) a polynucleotide sequence comprising at least 15 or 17 or 19 or 21 consecutive nucleotides of SEQ ID NO: 1, wherein the ingestion, by Coleoptera insect pests, of a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence inhibits the growth of the Coleoptera insect pests; or (c) any one of the polynucleotide sequences as shown in SEQ ID NO: 3 to SEQ ID NO: 6; or (d) a polynucleotide sequence hybridized with the polynucleotide sequence defined in (a), (b) or (c) mentioned above under stringent conditions. The present invention discloses multiple target sequences of the target gene c46312 for controlling the Coleoptera insect pest Monolepta hieroglyphica for the first time.
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01N 57/16 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
Disclosed by the present invention are an isolated polynucleotide and method used for controlling insect invasion, the isolated polynucleotide comprising: (a) a polynucleotide sequence as shown in SEQ ID NO: 1; or (b) a polynucleotide sequence having at least 15, 17, 19 or 21 contiguous nucleotides of SEQ ID NO: 1, wherein the growth of a pest of the order Coleoptera is inhibited when the pest of the order Coleoptera ingests double-stranded RNA comprising at least one strand that is complementary to the described polynucleotide sequence; or (c) any polynucleotide sequence as shown in SEQ ID NO: 3 to SEQ ID NO: 6; or (d) a polynucleotide sequence which hybridizes under stringent conditions with the polynucleotide sequence defined in (a), (b) or (c). Disclosed by the present invention for the first time is a plurality of target sequences used for controlling a target gene c4506 of Monolepta hieroglyphica, which is a pest of the order Coleoptera.
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01N 57/16 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
A01N 57/16 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
35.
POLYNUCLEOTIDE AND METHOD FOR CONTROLLING INSECT INVASION
Provided are an isolated polynucleotide and a method for controlling insect invasion. The isolated polynucleotide is a plurality of target sequences for controlling target gene c35112 of a coleopteran pest, Monolepta hieroglyphica, comprising: a) a polynucleotide sequence shown as SEQ ID NO: 1; or (b) a polynucleotide sequence having at least 15 or 17 or 19 or 21 contiguous nucleotides shown as SEQ ID NO: 1, double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence being capable of inhibiting the growth of coleopteran pests after being ingested by the coleopteran pests; or (c) any one of polynucleotide sequences shown as SEQ ID NO: 3 to SEQ ID NO: 20; or (d) a polynucleotide sequence that hybridizes under stringent conditions to a polynucleotide sequence as defined in (a), (b) or (c).
Provided are a resistant protein for use in herbicide dicamba, encoding gene and application thereof, the gene comprising: (a) a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO: 2; or (b) a nucleotide sequence which is complementary to the nucleotide sequence as defined by (a) under stringent conditions; or (c) a nucleotide sequence as shown in SEQ ID NO: 1.
The present invention relates to an herbicide tolerance protein, an encoding gene thereof and use thereof, the herbicide tolerance protein comprising: a protein (a) having an amino acid sequence as shown in SEQ ID NO: 1, and having an alanine substitution at least at position 176 and/or having a valine substitution at position 178 of SEQ ID NO: 1; or (b) having an amino acid sequence as shown in SEQ ID NO: 3; or (c) having an amino acid sequence as shown in SEQ ID NO: 5; or (d) having an amino acid sequence as shown in SEQ ID NO: 7; or (e) being derived from (a) by means of the amino acid sequence of (a) undergoing substitution and/or deletion and/or by added one or several amino acids, and having the activity of thifensulfuron hydrolase. The herbicide tolerance protein of the present invention has a broad application prospects in plants.
A herbicide tolerant protein SUM1, a coding gene thereof and a use thereof, wherein the herbicide tolerant protein comprises: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 1; or (b) a protein which is derived from (a) by substituting and/or deleting and/or adding one or more amino acids in the amino acid sequence of (a), and has the activity of thifensulfuron hydrolase. The herbicide tolerant protein SUM1 can show a higher tolerance to a plurality of sulfonylurea herbicides, can tolerate four-fold field concentration, and thus has a broad application prospect in plants.
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
A01N 47/36 - Ureas or thioureas containing the groups N—CO—N or N—CS—N containing the group N—CO—N directly attached to at least one heterocyclic ringThio-analogues thereof
C07K 14/395 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi from yeasts from Saccharomyces
39.
METHOD FOR IMPROVING TRANSFORMATION EFFICIENCY OF SOYBEAN
Provided is a method for improving the transformation efficiency of soybean, the method comprising: transforming plant cells by using a recombinant vector containing a target gene and a gene encoding a sulfonylurea-type herbicide hydrolase; selectively cultivating the transformed plant cells by externally applying an ALS inhibitor, with the gene encoding the sulfonylurea-type herbicide hydrolase acting as a selective marker; and selecting plant cells which are not killed and/or not inhibited. By adding a selection agent into a proliferation medium and a differential medium by externally applying same in the plant transformation process, the effective screening concentration range of the selection agent is optimized, such that the transformation efficiency and the proportion of positive plants obtained by the progeny thereof are significantly improved; in addition, transgenic plants obtained by means of transformation with the sulfonylurea-type herbicide hydrolase gene acting as the selective marker in the present invention have good resistance and genetic stability.
Involved is a herbicide-resistant protein, coding gene and use thereof. The herbicide-resistant protein comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein with the activity of herbicide-resistance which is derived from the amino acid sequence in (a) by replacing and/or deleting and/or adding one or several amino acids in the same. The herbicide-resistant protein of this invention is especially suitable for expression in plants, with broad resistance spectrum to herbicides, especially to phenoxy auxin herbicides.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Tao, Qing
Yang, Xu
Li, Jianyong
Abstract
An insecticidal protein combination, and insect resistance management method for same. The insect resistance management method comprises enabling Ostrinia furnacalis to contact at least the protein Cry2Ab and the protein Cry1Ac. By employing two types of insecticidal proteins, Cry1Ac and Cry2Ab, to effectively delay or prevent Ostrinia furnacalis from having resistance, the present invention realizes pest control of Ostrinia furnacalis, provides superior protection of plants, and stabilizes yields.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Tao, Qing
Yang, Xu
Li, Jianyong
Abstract
An insecticidal protein combination, and insect resistance management method for same. The insect resistance management method comprises enabling Ostrinia furnacalis to contact at least the protein Cry2Ab and the protein Cry1Ab. By employing two types of insecticidal proteins, Cry1Ab and Cry2Ab, to effectively delay or prevent Ostrinia furnacalis from having resistance, the present invention realizes pest control of Ostrinia furnacalis, provides superior protection of plants, and stabilizes yields.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
29 - Meat, dairy products, prepared or preserved foods
30 - Basic staples, tea, coffee, baked goods and confectionery
31 - Agricultural products; live animals
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Soil conditioning preparations; nitrogenous fertilizers; calcium cyanamide; fertilizers; fertilizing preparations; plant growth regulating preparations; chemical fertilizer; fertilizer for household plants; organic fertilizers; soil amendments Preparations for destroying noxious animals; insecticides; insect repellents; herbicides; herbicides for agricultural use; herbicides (weedkillers); soil-sterilising preparations; preparations for destroying vermin; pesticides; fungicides for agricultural use Edible fats; oils for food; processed oils and fats for food; corn oil for food; processed edible seeds, not being seasonings or flavorings; albumen for culinary purposes; tofu; deep fried tofu (atsu-age) Preparations made from cereals, namely, corn-based snack foods; corn flour; soya flour; groats for human food; corn, milled; corn, roasted; grain-based chips; hominy; corn syrup; popcorn; starch for food Raw popcorn (unprocessed kernels); raw beans; fresh bean; cereal seeds, unprocessed; seed balls for planting; seeds for planting; seeds for sowing; crop seeds; seeds for agricultural purposes; animal foodstuffs; oil cake; grains for animal consumption; cattle food; algarovilla for animal consumption Research and development of technology in the field of agriculture; scientific study and research in the field (s) of agriculture; scientific laboratory services; scientific research; technical writing; scientific research and development; biological research Vermin exterminating for agriculture, aquaculture, horticulture and forestry; pest control services for agriculture, aquaculture, horticulture and forestry
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
29 - Meat, dairy products, prepared or preserved foods
30 - Basic staples, tea, coffee, baked goods and confectionery
31 - Agricultural products; live animals
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Soil conditioning preparations; nitrogenous fertilizers; calcium cyanamide; fertilizers; fertilizing preparations; plant growth regulating preparations; chemical fertilizer; fertilizers for household plants; organic fertilizers; soil amendments Preparations for destroying noxious animals; insecticides; insect repellents; herbicides; herbicides for agricultural use; herbicides (weedkillers); soil-sterilising preparations; preparations for destroying vermin; pesticides; fungicides for agricultural use Edible fats; oils for food; processed oils and fats for food; corn oil for food; processed edible seeds, not being seasonings or flavorings; albumen for culinary purposes; tofu; deep fried tofu (atsu-age) Preparations made from cereals, namely, corn; corn flour; soya flour; groats for human food; corn, milled; corn, roasted; grain-based chips; hominy; corn syrup; popcorn; starch for food Raw popcorn (unprocessed kernels); raw beans; fresh bean; cereal seeds, unprocessed; seed balls for plantings; seeds for planting; seeds for sowing; crop seeds; seeds for agricultural purposes; animal foodstuffs; oil cake; grains for animal consumption; cattle food; algarovilla for animal consumption Research and development of technology in the field of agriculture; scientific study and research in the field (s) of agriculture; scientific laboratory services; scientific research; technical writing; scientific research and development; biological research Vermin exterminating for agriculture, aquaculture, horticulture and forestry; pest control services for agriculture, aquaculture, horticulture and forestry
47.
Herbicide-resistant protein, encoding gene and use thereof
Involved is a herbicide-resistant protein, coding gene and use thereof. The herbicide-resistant protein comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein with the activity of herbicide-resistance which is derived from the amino acid sequence in (a) by replacing and/or deleting and/or adding one or several amino acids in the same. The herbicide-resistant protein of this invention is especially suitable for expression in plants, with broad resistance spectrum to herbicides, especially to phenoxy auxin herbicides.
Involved is a herbicide-resistant protein, coding gene and use thereof. The herbicide-resistant protein comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein with the activity of herbicide-resistance which is derived from the amino acid sequence in (a) by replacing and/or deleting and/or adding one or several amino acids in the same. The herbicide-resistant protein of this invention is especially suitable for expression in plants, with broad resistance spectrum to herbicides, especially to phenoxy auxin herbicides.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
A01N 57/20 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
Provided are a resistant protein for use in herbicide dicamba, encoding gene and application thereof, the gene comprising: (a) a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO:2; or (b) a nucleotide sequence which is complementary to the nucleotide sequence as defined by (a) under a stringent condition; or (c) an amino acid sequence as shown in SEQ ID NO:1.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Wang, Dengyuan
Yu, Caihong
Zhang, Chengwei
Han, Chao
Li, Xiaojiao
Jiang, Ziqin
Zhang, Liangjun
Wu, Zhujun
Tian, Kangle
Bao, Xiaoming
Abstract
Provided is a nucleic acid sequence used for detecting the presence of a transgene soybean event DBN9008 in a biological sample, a test kit containing said nucleic acid sequence, and a detection method thereof.
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
A01N 57/20 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
NUCLEIC ACID SEQUENCE FOR DETECTING EXISTENCE OF TRANSGENIC SOYBEAN EVENT DBN9004 IN BIOLOGICAL SAMPLE, KIT CONTAINING SAME AND DETECTION METHOD THEREFOR
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Wang, Dengyuan
Yu, Caihong
Zhang, Chengwei
Han, Chao
Li, Xiaojiao
Jiang, Ziqin
Zhang, Liangjun
Wu, Zhujun
Tian, Kangle
Bao, Xiaoming
Abstract
Provided are a nucleic acid sequence for detecting the existence of a transgenic soybean event DBN9004 in a biological sample, a kit containing the same and a detection method therefor.
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
A01N 57/20 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
The present invention relates to the use of a herbicide-tolerant protein, wherein the method for controlling weeds comprises applying a herbicide containing an effective dose of tribenuron-methyl to a plant growth environment where at least one transgenic plant is present, wherein the transgenic plant comprises a nucleotide sequence encoding a thifensulfuron hydrolase in its genome, and compared to other plants without the nucleotide sequence encoding the hydrolase, the transgenic plant has reduced plant damage and/or an increased plant yield. The present invention discloses for the first time that a thifensulfuron hydrolase can show a high tolerance to a tribenuron-methyl herbicide, plants containing a nucleotide sequence encoding the thifensulfuron hydrolase are strongly tolerant to the tribenuron-methyl herbicide and can at least tolerate 1-fold field concentration, and thus the hydrolase has broad application prospects in plants.
A herbicide tolerant protein SUM1, an encoding gene and use thereof. Said herbicide tolerant protein includes: (a) a protein having an amino acid sequence as set forth in SEQ ID NO:1; or (b) a protein derived from (a) by means of substitution and/or deletion and/or addition of one or more amino acid(s) in the amino acid sequence of (a) and having hydrolase activity to thifensulfuron-methyl. The herbicide tolerant protein can exhibit higher tolerance to multiple sulfonylurea herbicides, can have tolerance up to four times the field concentration, and hence has broad application prospects on plants.
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
A01N 47/36 - Ureas or thioureas containing the groups N—CO—N or N—CS—N containing the group N—CO—N directly attached to at least one heterocyclic ringThio-analogues thereof
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Xie, Xiangting
Tao, Qing
Ding, Derong
Abstract
The present invention relates to an application for a herbicide-tolerant protein, a method for controlling weeds comprising applying a herbicide containing an effective dosage of halosulfuron-methyl to a plant growth environment containing at least one transgenic plant, the genome of the transgenic plant comprising a nucleotide sequence encoding thifensulfuron hydrolase; compared to other plants having a nucleotide sequence not encoding thifensulfuron hydrolase, the transgenic plant has reduced plant damage and/or increased plant yield. The present invention discloses for the first time that thifensulfuron hydrolase can show high tolerance to halosulfuron-methyl herbicide, and that plants containing a nucleotide sequence encoding thifensulfuron hydrolase have a high tolerance to halosulfuron-methyl herbicide and can tolerate at least double the field concentration, thus having wide prospects for application in plants.
A01N 57/20 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
A01N 47/36 - Ureas or thioureas containing the groups N—CO—N or N—CS—N containing the group N—CO—N directly attached to at least one heterocyclic ringThio-analogues thereof
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Xie, Xiangting
Tao, Qing
Pang, Jie
Ding, Derong
Bao, Xiaoming
Abstract
Provided is a method for weeds control, which comprises transferring a nucleotide sequence encoding thifensulfuron-methyl hydrolysis enzyme to a plant genome; when compared with a control plant, the transgenic plant has alleviated plant damage and/or increased plant yield; further applying a herbicide containing an effective amount of tribenuron-methyl to a growing environment of the transgenic plant, as the thifensulfuron-methyl hydrolysis enzyme has a relatively high tolerance to the tribenuron-methyl herbicide, weeds control can be achieved.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Xie, Xiangting
Tao, Qing
Pang, Jie
Abstract
Use of a herbicide tolerant protein and a method for controlling weeds. The method for controlling weeds includes the step of applying a herbicide having an effective dose of sulfometuron-methyl to a plant growing environment in which at least one transgenic plant is present. The transgenic plant comprises a nucleotide sequence encoding thifensulfuron-methyl hydrolase in its genome. Compared to other plants without the nucleotide sequence encoding thifensulfuron-methyl hydrolase, the transgenic plant sustains mitigated damage and/or an increased plant yield. The thifensulfuron-methyl hydrolase can exhibit higher tolerance to the sulfometuron-methyl herbicide, and the plant having the nucleotide sequence encoding the thifensulfuron-methyl hydrolase has strong tolerance to the sulfometuron-methyl herbicide and can at least withstand one times the field concentration.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Xie, Xiangting
Tao, Qing
Bao, Xiaoming
Abstract
A herbicide tolerant protein SUM1, an encoding gene and use thereof. Said herbicide tolerant protein includes: (a) a protein having an amino acid sequence as set forth in SEQ ID NO:1; or (b) a protein derived from (a) by means of substitution and/or deletion and/or addition of one or more amino acid(s) in the amino acid sequence of (a) and having hydrolase activity to thifensulfuron-methyl. The herbicide tolerant protein can exhibit higher tolerance to multiple sulfonylurea herbicides, can have tolerance up to four times the field concentration, and hence has broad application prospects on plants.
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
A01N 47/36 - Ureas or thioureas containing the groups N—CO—N or N—CS—N containing the group N—CO—N directly attached to at least one heterocyclic ringThio-analogues thereof
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Ding, De'Rong
Zhang, Aihong
Abstract
Disclosed are a nucleotide sequence and the method thereof for controlling an insect infestation, wherein the isolated nucleotide sequence comprises: (a) the polynucleotide sequence as shown in SEQ ID NO: 1, 2 or 3; or (b) a polynucleotide sequence hybridized with the polynucleotide sequence defined in (a) under stringent conditions; or (c) a polynucleotide sequence having at least 80% identity to the polynucleotide sequence defined in (a); or (d) a polynucleotide sequence of at least 19 consecutive nucleotides of the polynucleotide sequence defined in (a), wherein a Hemiptera insect pest ingests a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence, thus inhibiting the growth of the Hemiptera insect pest; or (e) a complementary sequence of the polynucleotide sequence as defined in the above-mentioned (a), (b), (c) or (d). The three target sequences for controlling a Hemiptera insect pest of Lygus have the advantages of high efficiency, specificity and convenience, and a low cost.
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
A01N 57/16 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Zhang, Aihong
Yang, Xu
Abstract
Provided in the present invention is the use of a pesticidal protein for controlling Chilo sacchariphagus, comprising: contacting Chilo sacchariphagus at least with Vip3A protein, and controlling Chilo sacchariphagus by producing the Vip3A protein in plants which can kill Chilo sacchariphagus. The present invention can protect the whole plant throughout the whole growth period.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Yang, Xu
Zhang, Aihong
Abstract
An application of an insecticidal protein. A chilo sacchariphagus injurious insect is controlled by generating Cry1A protein capable of killing the chilo sacchariphagus in a plant body. A method for controlling a chilo sacchariphagus injurious insect comprises: the chilo sacchariphagus injurious insect at least contacts Cry1A protein. Compared with an agricultural prevention and control method, a chemical prevention and control method and a physical prevention and control method used in the prior art, the insecticidal protein protects a whole plant in a whole growth period so as to prevent and control the damage of the chilo sacchariphagus injurious insect, has no pollution and no residue, is stable and thorough in effect, and is simple, convenient and economical.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Yang, Xu
Zhang, Aihong
Abstract
The present invention relates to an application of an insecticidal protein. A method for controlling a chilo sacchariphagus injurious insect comprises: the chilo sacchariphagus injurious insect at least contacts Cry2Ab protein. The present invention controls a chilo sacchariphagus injurious insect by generating Cry2Ab proteins capable of killing the chilo sacchariphagus in a plant body; compared with an agricultural prevention and control method, a chemical prevention and control method and a physical prevention and control method used in the prior art, the present invention protects a whole plant in a whole growth period so as to prevent and control the damage of the chilo sacchariphagus injurious insect, has no pollution and no residue, is stable and thorough in effect and is simple, convenient and economical.
A01H 4/00 - Plant reproduction by tissue culture techniques
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
A01N 37/18 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N, e.g. carboxylic acid amides or imidesThio-analogues thereof
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
A01N 57/20 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Ding, Derong
Kang, Yuejing
Zhang, Yunzhu
Liu, Haili
Pang, Jie
Wang, Lijun
Jia, Zhiwei
Huang, Jincun
Guo, Hanzi
Wang, Lei
Fu, Xueqian
Zhou, Yi
Li, Feng
Bao, Xiaoming
Lu, Yuping
Zhang, Shiping
Abstract
Provided is a transgenic maize event DBN9978. A maize plant comprising the event is resistant to Lepidopteran insects and is tolerant to a glyphosate herbicide. Also provided are a nucleic acid sequence used for detecting in a biological sample the presence of maize event DBN9978 and a detection method therefor.
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
A01N 57/20 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
A01N 57/20 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
C07K 14/71 - ReceptorsCell surface antigensCell surface determinants for growth factorsReceptorsCell surface antigensCell surface determinants for growth regulators
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
A61K 38/17 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Han, Chao
Wang, Lijun
Yu, Caihong
Yue, Jianting
Abstract
Uses of insecticidal protein and a method for controlling chilo suppressalis insects. The method comprises: allowing chilo suppressalis insects to be at least in contact with a Vip3A protein. The chilo suppressalis insects are controlled by means of the Vip3A protein that is generated in plant bodies and that can kill chilo suppressalis insects. Compared with the agricultural control method, the chemical control method and the physical control method used in the prior art, the whole plants are protected at the total growth period so as to control invasion of the chilo suppressalis insects, and the insecticidal protein is pollution-free, residue-free, stable and thorough in effect, simple, convenient and economical.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Li, Jianyong
Yang, Xu
Li, Mei
Yu, Caihong
Abstract
Provided is a method for controlling prodenia litura. The method comprises: allowing prodenia litura to be in contact with a Cry2Ab protein. The method specifically comprises: growing genetically modified plants by using polynucleotide coded with a Cry2Ab protein; and allowing the prodenia litura to be in contact with the Cry2Ab protein by allowing the prodenia litura to eat genetically modified plants, wherein after the contact, the prodenia litura is restrained in growth and/or finally dies, so as to prevent the prodenia litura doing harm to plants.
Provided are a protein having herbicide resistance and an encoding gene and an application thereof. The protein comprises: (a) a protein composed of an amino acid sequence shown by SEQ ID NO:2; or (b) a protein that is derived from (a) and provided with herbicide resistance activity through replacement and/or deficiency and/or addition of one or more amino acids of the amino acid sequence in (a). The protein having herbicide resistance is suitable for expression in plants, and can improve the resistance of plants to 2,4-dichlorophenoxyacetic acid (2,4-D).
A01G 7/06 - Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
The present invention provides herbicide resistance protein, and encoding genes and application thereof. The herbicide resistance protein comprises: protein (a) composed of an amino acid sequence shown in SEQ ID NO:2, or protein (b) derived by (a) through replacement and/or deficiency and/or addition of one or more amino acid of the amino acid sequence in (a) and provided with herbicide resistance activity. The herbicide resistance protein in the present invention is particularly suitable for expression in plants, wide in herbicide resistance, and particularly used in phenoxy auxin herbicide.
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Tao, Qing
Wu, Yechun
Niu, Xiaoguang
Xie, Xiangting
Pang, Jie
Bao, Xiaoming
Abstract
Provided are herbicide resistance protein, and encoding genes and an application thereof. The herbicide resistance protein comprises: protein (a) composed of an amino acid sequence shown in SEQ ID NO:2, or protein (b) derived by (a) through replacement and/or deficiency and/or addition of one or more amino acid of the amino acid sequence in (a) and provided with herbicide resistance activity. The herbicide resistance protein has particularly good resistance to phenoxy auxin herbicide.
Provided are herbicide resistance protein, and encoding genes and an application thereof. The herbicide resistance protein comprises: protein (a) composed of an amino acid sequence shown in SEQ ID NO:2, or protein (b) derived by (a) through replacement and/or deficiency and/or addition of one or more amino acid of the amino acid sequence in (a) and provided with herbicide resistance activity. The herbicide resistance protein has particularly good resistance to phenoxy auxin herbicide.
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Wu, Yechun
Niu, Xiaoguang
Tao, Qing
Pang, Jie
Abstract
Provided are a protein having herbicide resistance and an encoding gene and an application thereof. The protein comprises: (a) a protein composed of an amino acid sequence shown by SEQ ID NO:2; or (b) a protein that is derived from (a) and provided with herbicide resistance activity through replacement and/or deficiency and/or addition of one or more amino acids of the amino acid sequence in (a). The protein having herbicide resistance is suitable for expression in plants, and can improve the resistance of plants to 2,4-dichlorophenoxyacetic acid (2,4-D).
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Tao, Qing
Wu, Yechun
Niu, Xiaoguang
Ding, Derong
Abstract
The present invention provides herbicide resistance protein, and encoding genes and application thereof. The herbicide resistance protein comprises: protein (a) composed of an amino acid sequence shown in SEQ ID NO:2, or protein (b) derived by (a) through replacement and/or deficiency and/or addition of one or more amino acid of the amino acid sequence in (a) and provided with herbicide resistance activity. The herbicide resistance protein in the present invention is particularly suitable for expression in plants, wide in herbicide resistance, and particularly used in phenoxy auxin herbicide.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Yang, Xu
Ding, Derong
Zhang, Aihong
Tao, Qing
Li, Jianyong
Li, Mei
Zhang, Yunzhu
Abstract
The present invention relates to uses of an insecticidal protein. The method for controlling Chilo infuscatellus pests comprises: bringing Chilo infuscatellus pests into contact with Cry1A protein. The present invention controls Chilo infuscatellus pests by means of Cry1A protein that is produced in vivo by a plant and capable of killing Chilo infuscatellus. Compared with the prior art in which agricultural prevention methods, chemical prevention methods, and physical prevention methods are used, the present invention provides the plant with protection throughout the growth period and for the entire plant to prevent damages due to Chilo infuscatellus pests, is pollution-free and residue-free, provides stable and thorough effects, and is simple, convenient, and economical.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Yang, Xu
Ding, Derong
Zhang, Xinxin
Kang, Yuejing
Abstract
The present invention relates to uses of an insecticidal protein. The method for controlling Chilo infuscatellus pests comprises: bringing Chilo infuscatellus pests into contact with Cry1A.105 protein. The present invention controls Chilo infuscatellus pests by means of Cry1A.105 protein that is produced in vivo by a plant and capable of killing Chilo infuscatellus. Compared with the prior art in which agricultural prevention and treatment methods, chemical prevention and treatment methods, and physical prevention and treatment methods are used, the present invention provides the plant with protection throughout the growth period and for the entire plant to prevent damages due to Chilo infuscatellus pests, is pollution-free and residue-free, provides stable and thorough effects, and is simple, convenient, and economical.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Zhang, Aihong
Bao, Xiaoming
Liu, Haili
Pei, Yanxin
Zhang, Yunzhu
Abstract
Provided is a method for controlling Prodenia litura, wherein the Prodenia litura comes into contact with a Cry1F protein. The method comprises producing a transgenic plant using the Cry1Fa protein, the Prodenia litura comes into contact with the Cry1Fa protein by eating the transgenic plant, then the growth of the Prodenia litura after contact is suppressed and/or they finally die therefrom, thereby achieving the control of Prodenia litura which are harming the plants.
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD. (China)
BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (China)
Inventor
Kang, Yuejing
Wang, Dengyuan
Jiao, Guowei
Tian, Cong
Zhang, Yunzhu
Abstract
A method for controlling spodoptera litura pests, the spodoptera litura pests contacting the Vip3A protein; and the spodoptera litura pests are controlled via the Vip3A protein generated in a plant and capable of killing the spodoptera litura.
A01N 57/16 - Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
Some embodiments of the present invention can include herbicide-resistant proteins, coding genes, and uses thereof. Certain herbicide-resistant proteins can comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein consisting of an amino acid sequence at least 90% identical to that set forth in SEQ ID NO: 2. Other herbicide-resistant proteins of this disclosure can be suitable for expression in plants, can have resistance to herbicides (e.g., to phenoxy auxinherbicides), or both.
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
Some embodiments of the present invention can include herbicide-resistant proteins, coding genes, and uses thereof. Certain herbicide-resistant proteins can comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein consisting of an amino acid sequence at least 90% identical to that set forth in SEQ ID NO: 2. Other herbicide-resistant proteins of this disclosure can be suitable for expression in plants, can have resistance to herbicides (e.g., to phenoxy auxinherbicides), or both.
Involved is a herbicide-resistant protein, coding gene and use thereof. The herbicide-resistant protein comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein consisting of an amino acid sequence at least 90% identical to that set forth in SEQ ID NO: 2. The herbicide-resistant protein of this application is especially suitable for expression in plants, with broad resistance spectrum to herbicides, especially to phenoxy auxin herbicides.
A01N 25/32 - Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
Involved is a herbicide-resistant protein, coding gene and use thereof. The herbicide-resistant protein comprises: (a) a protein consisting of an amino acid sequence shown in SEQ. ID NO: 2; or (b) a protein consisting of an amino acid sequence at least 90% identical to that set forth in SEQ ID NO: 2. The herbicide-resistant protein of this application is especially suitable for expression in plants, with broad resistance spectrum to herbicides, especially to phenoxy auxin herbicides
The invention relates to an insecticidal protein, its gene encoding and the uses thereof. The protein comprises: (a) a protein consisting of an amino acid sequence shown by SEQ ID NO:2; or (b) a protein derived from (a) consisting of an amino acid sequence by substitution, deletion, or addition of one or more amino acid residues of the amino acid sequences in (a), and having insecticidal activity; or (c) a protein generated by the expression of nucleic acid molecules containing a nucleotide sequence of SEQ ID NO:1; or (d) a protein generated by the expression of nucleic acid molecules containing a complementary sequence that hybridized with SEQ ID NO:1 under stringent conditions; or (e) a protein generated by the expression of nucleic acid molecules that contain nucleotide sequences isocoding with the nucleotide sequences in (d). The insecticidal protein of the invention has high expression level and strong toxicity against pests.
A01N 37/44 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio-analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio-analogue of a carboxylic group, e.g. amino-carboxylic acids
A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
C07K 14/325 - Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
The invention relates to an insecticidal protein, its gene encoding and the uses thereof. The protein comprises: (a) a protein consisting of an amino acid sequence shown by SEQ ID NO:2 or a sequence of amino acid residuses at positions 1 to 640 thereof; or (b) a protein derived from (a) by substitution, deletion, or addition of one or more amino acid residuses of the amino acid sequences in (a), and having insecticidal activity; or (c) a protein generated by the expression of nucleic acid molecules containing a sequence of amino acid residuses at the positions 1 to 1920 of SEQ ID NO:1; or (d) a protein generated by the expression of nucleic acid molecules containing a complementary sequence that hybridized with the sequence of nucleotides at the positions 1 to 1920 of SEQ ID NO:1 under stringent conditions. The protein has high expression level and strong toxicity against pests.
A01N 37/44 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio-analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio-analogue of a carboxylic group, e.g. amino-carboxylic acids
A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
C07K 14/325 - Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
A01N 63/02 - Substances produced by, or obtained from, microorganisms or animals
