Abstract

This technology relates in part to methods of identifying plant cultivars based on the terpene synthase genes that are identified and/or quantified (e.g., copy number, ploidy) in the cultivars. The methods provided herein permit plant cultivars with desired characteristics/phenotypes, e.g., a desired terpene production profile, to be selected for use in various applications, such as agriculture (e.g., selecting cultivars for breeding desired characteristics and/or lineages) and medicine (e.g., therapeutic activity).

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells

Abstract

This technology relates in part to methods and compositions for identifying and/or selecting one or more terpene synthase genes and/or variants thereof in a plant, based on which a plant with a desired terpene profile can be obtained and/or used. A desired terpene profile of a plant (i.e., the types and, optionally, the relative amounts of terpenes in a plant) can include, for example, an energetic terpene profile, an anti-nociceptive terpene profile and an insecticidal terpene profile.

IPC Classes  ?

• A01H 1/00 - Processes for modifying genotypes
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
• C12Q 1/6851 - Quantitative amplification

Abstract

The unique annual herbaceous Cannabis plant variety ‘LPA004’ is provided.

IPC Classes  ?

• A01H 6/28 - Cannabaceae, e.g. cannabis
• A01H 5/02 - Flowers
• A01H 5/10 - Seeds
• A01H 5/12 - Leaves

Abstract

cannabis plants. In some aspects, the technology relates to methods and compositions for classifying a hops latent viroid genotype. In certain aspects, the technology relates to methods and compositions for determining the presence, absence and/or amount of one or more pathogens in plants, either independently or simultaneously. In aspects, the pathogen is a virus. In some aspects, the virus is selected from among one or more of hops latent viroid, beet curly top virus and alfalfa mosaic virus.

IPC Classes  ?

• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage

Abstract

This technology relates in part to methods of identifying plant cultivars based on the terpene synthase genes that are identified and/ or quantified (e.g., copy number, ploidy) in the cultivars. The methods provided herein permit plant cultivars with desired characteristics/ phenotypes, e.g., a desired terpene production profile, to be selected for use in various applications, such as agriculture (e.g., selecting cultivars for breeding desired characteristics and/or lineages) and medicine (e.g., therapeutic activity). Preferred plants are Cannabis plants, in particular Cannabis sativa.

IPC Classes  ?

• A01H 1/04 - Processes of selection
• A01H 6/28 - Cannabaceae, e.g. cannabis
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

This technology relates in part to methods of identifying plant cultivars based on the terpene synthase genes that are identified and/ or quantified (e.g., copy number, ploidy) in the cultivars. The methods provided herein permit plant cultivars with desired characteristics/ phenotypes, e.g., a desired terpene production profile, to be selected for use in various applications, such as agriculture (e.g., selecting cultivars for breeding desired characteristics and/or lineages) and medicine (e.g., therapeutic activity). Preferred plants are Cannabis plants, in particular Cannabis sativa.

IPC Classes  ?

• A01H 1/04 - Processes of selection
• A01H 6/28 - Cannabaceae, e.g. cannabis
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

The technology relates in part to methods and compositions for detecting one or more pathogens in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in cannabis plants. In some aspects, the technology relates to methods and compositions for classifying a hops latent viroid genotype. In certain aspects, the technology relates to methods and compositions for determining the presence, absence and/or amount of one or more pathogens in plants, either independently or simultaneously. In aspects, the pathogen is a virus. In some aspects, the virus is selected from among one or more of hops latent viroid, beet curly top virus and alfalfa mosaic virus.

IPC Classes  ?

• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage

Abstract

The technology relates in part to methods and compositions for detecting one or more pathogens in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in cannabis plants. In some aspects, the technology relates to methods and compositions for classifying a hops latent viroid genotype. In certain aspects, the technology relates to methods and compositions for determining the presence, absence and/or amount of one or more pathogens in plants, either independently or simultaneously. In aspects, the pathogen is a virus. In some aspects, the virus is selected from among one or more of hops latent viroid, beet curly top virus and alfalfa mosaic virus.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage

Abstract

The technology relates in part to methods and compositions for detecting one or more pathogens in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in cannabis plants. In some aspects, the technology relates to methods and compositions for classifying a hops latent viroid genotype. In certain aspects, the technology relates to methods and compositions for determining the presence, absence and/or amount of one or more pathogens in plants, either independently or simultaneously. In aspects, the pathogen is a virus. In some aspects, the virus is selected from among one or more of hops latent viroid, beet curly top virus and alfalfa mosaic virus.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage

Abstract

Provided herein are systems, methods and kits for micropropagating disease-free cannabis plants, referred to as the Clean Stock® method. Also provided are Clean Stock® cannabis plants produced using such systems, methods and kits.

IPC Classes  ?

• A01H 6/28 - Cannabaceae, e.g. cannabis
• A01H 4/00 - Plant reproduction by tissue culture techniques

Abstract

Provided herein are systems, methods and kits for micropropagating disease-free cannabis plants, referred to as the Clean Stock® method. Also provided are Clean Stock® cannabis plants produced using such systems, methods and kits.

IPC Classes  ?

• A01H 4/00 - Plant reproduction by tissue culture techniques
• A01H 5/02 - Flowers
• A01H 6/28 - Cannabaceae, e.g. cannabis

Abstract

Provided herein are systems, methods and kits for micropropagating disease-free cannabis plants, referred to as the Clean Stock® method. Also provided are Clean Stock® cannabis plants produced using such systems, methods and kits.

IPC Classes  ?

• A01H 4/00 - Plant reproduction by tissue culture techniques
• A01H 5/02 - Flowers
• A01H 6/28 - Cannabaceae, e.g. cannabis