Abstract

An epigenetic regulatory factor ZmULT1 related to corn grain size regulation and heat stress response and the use thereof, which solve the technical problems of how to increase the grain size and/or heat resistance and/or lodging resistance of corn. Use of a substance for reducing the content of the protein ZmULT1 or a substance for inhibiting the expression of the encoding gene of the protein ZmULT1, which use is any one of the following: P1, the use in increasing the grain size of corn; P2, the use in improving the heat resistance of corn; P3, the use in increasing the lodging resistance of corn; and P4, the use in plant breeding. The homozygous mutant strain with knockout of the ZmULT1 gene has a reduced plant height and an increased grain size, and exhibits significant heat stress resistance, this indicates that the research on the gene can further enrich the use of epigenetics in crops and is of important significance for improving the grain size, heat resistance and lodging resistance of corn.

IPC Classes  ?

• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/84 - Ti-plasmids
• A01H 5/10 - Seeds
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize

Abstract

A use of a composition in detecting N6-methyladenosine modification in mRNA bound with a plant protein. The composition is composed of ethanol, sodium acetate, and glycogen, and, in the composition, the ratio of ethanol to sodium acetate to glycogen is 500 ul of ethanol to 3 mol of sodium acetate to 0.1 g of glycogen.

IPC Classes  ?

• G01N 30/02 - Column chromatography
• G01N 30/06 - Preparation
• G01N 30/60 - Construction of the column
• G01N 30/72 - Mass spectrometers

Abstract

A transcription detection method for identifying downy mildew resistance of soybean germplasm. The transcription detection method for identifying the downy mildew resistance of the soybean germplasm comprises using an SAGT1 gene specific primer to measure the expression level of an SAGT1 gene in a soybean, using a PR1 gene specific primer to measure the expression level of a PR1 gene in the soybean, and determining the downy mildew resistance of the soybean on the basis of the expression levels of the SAGT1 and PR1 genes. Experiments prove that the transcription expression levels of the SAGT1 and PR1 genes are highly positively correlated with the soybean downy mildew resistance of soybeans, the consistency of a disease resistance result of soybeans detected by utilizing the expression levels of the SAGT1 and PR1 genes and a field identification result is as high as 70.00%, and the present invention can be used for identifying and screening the downy mildew resistance of soybean germplasm resources.

IPC Classes  ?

• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
• C12Q 1/6851 - Quantitative amplification

Abstract

The present application discloses a method for improving density-tolerant yield of maize and use thereof, belongs to the field of plant biotechnology breeding. Compared with the Ig1 mutant strain, the LG1/Ig1 heterozygous genotype strain has excellent agronomic characters such as a widened leaf, a larger spike, a reduced empty stalk ratio and/or an increased yield by close planting; compared with the LG1 wild-type strain, the LG1/Ig1 heterozygous genotype material reduces a leaf angle and tassel branch number of maize, and has the effect of increasing the yield by close planting. The method has a wide application prospect in the field of maize high-yield breeding.

IPC Classes  ?

• A01H 1/00 - Processes for modifying genotypes

Abstract

Provided are ZmYUC2 and ZmYUC4 genes for regulating and controlling an included angle and the lodging resistance of a maize root system, and use thereof. The ZmYUC2 and ZmYUC4 genes can specifically regulate and control local auxin synthesis of a root tip, and can regulate and control the included angle of the maize root system without an adverse effect on the remaining agronomic traits, so that the ZmYUC2 and ZmYUC4 genes can be applied to a lodging-resistant breeding variety.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 9/22 - Ribonucleases

Abstract

Disclosed are a method for improving maize yield in a density-tolerant manner and the use thereof, which belong to the field of plant biotechnical breeding. The homozygous lg1 mutant has adverse agronomic traits such as small ears, low yield and high empty stem rate. Provided is a new density-tolerant breeding method. An LG1/lg1 heterozygous genotype material is obtained by hybridizing an lg1 mutant strain with an LG1 wild-type strain. Compared with the LG1 wild-type strain, the LG1/lg1 heterozygous genotype material reduces the maize leaf angle and the tassel branch number, and increases the yield under high density conditions; compared with the lg1 mutant, the LG1/lg1 heterozygous genotype material has excellent agronomic traits such as wider leaves, larger ears, reduced empty stem rate, dense-planting increased yield; and the breeding method has wide application prospects in a density-tolerant and high-yield maize breeding field.

IPC Classes  ?

• A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
• A01H 1/00 - Processes for modifying genotypes
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize

Abstract

Disclosed in the present invention are a DMP protein and a coding gene and use thereof. Disclosed is a complete set of proteins consisting of protein A (i.e. DMP8) and protein B (i.e. DMP9). The amino acid sequence of protein A is SEQ ID No. 1, and the amino acid sequence of protein B is SEQ ID No. 2. Also disclosed in the present invention are a method for constructing a plant haploid inducer line and a use thereof, and a plant haploid inducer line constructed by the method.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 9/22 - Ribonucleases
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

Disclosed in embodiments of the present application are a method for improving agronomic traits of plants and the use thereof. By means of overexpressing functional gene ZmSPL12 in plants, the salt stress resistance, alkali stress resistance and/or resistance to low nitrogen of plants are improved. The method and the use thereof provide the possibility for cultivating new salt-alkali resistant, and low-nitrogen resistant crop varieties, especially new corn varieties, and are of great significance for global grain safety and agricultural sustainable development.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

Provided are an expression vector of glyphosate-resistant genes GR79 and GAT, a high glyphosate-resistant corn, and a detection method therefor. The codons of the GR79 gene and GAT gene which have high tolerance to glyphosate are optimized and new DNA sequences are synthesized; meanwhile, a dual-gene plant expression vector pCGG is constructed. Results show that a transgenic corn transformed with the plant expression vector has high resistance to target herbicide glyphosate. The transgenic corn GG2 is a transformation event having a significant glyphosate tolerance effect, and a left border flanking sequence and a right border flanking sequence thereof are obtained by means of a chromosome walking method. The border sequences at two ends can be used as specific detection sequences for the present transformation event. Primers designed on the basis of the two border sequences can specifically detect the transgenic event GG2 and be applied to the development of a detection kit.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

Provided are ZmYUC2 and ZmYUC4 genes for regulating and controlling an included angle and the lodging resistance of a corn root system, and use thereof. The ZmYUC2 and ZmYUC4 genes can specifically regulate and control local auxin synthesis of a root tip, and can regulate and control the included angle of the corn root system without an adverse effect on the remaining agronomic traits, so that the ZmYUC2 and ZmYUC4 genes can be applied to a lodging-resistant breeding variety.

IPC Classes  ?

• C12N 15/53 - Oxidoreductases (1)
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy

Abstract

Provided are an insect-resistant and herbicide-resistant transgenic corn and a cultivation method therefor. The method comprises combining the mCry1Ab, mCry1F and mCP4EPSPS genes into the form of a gene tandem expression cassette, and integrating same into a receptor corn material. Therefore, compared with a single-gene insect-resistant or herbicide-resistant gene used in the prior art, the resistance of the transgenic corn against lepidopterans is improved, and the tolerance of the transgenic corn to glyphosate also reaches a high resistance level.

IPC Classes  ?

• C12N 15/62 - DNA sequences coding for fusion proteins
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 15/84 - Ti-plasmids
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• C12R 1/01 - Bacteria or actinomycetales

Abstract

The present invention belongs to the technical field of plant biology. Disclosed are an insect-resistant and herbicide-resistant transgenic corn and a cultivation method therefor. According to the present invention, the method comprises combining the mCry1Ab, mCry3Bb and mCP4EPSPS genes into the form of a gene tandem expression cassette, and integrating same into a receptor corn material. Therefore, compared with a single-gene insect-resistant or herbicide-resistant gene used in the prior art, the resistance of the transgenic corn against lepidopterans and coleopterans is significantly improved, and the tolerance of the transgenic corn to glyphosate also reaches a high resistance level.

IPC Classes  ?

• C12N 15/62 - DNA sequences coding for fusion proteins
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 15/84 - Ti-plasmids
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• C12R 1/01 - Bacteria or actinomycetales

Abstract

Disclosed in the present invention are a DMP protein and a coding gene and use thereof. Disclosed is a complete set of proteins consisting of protein A (i.e. DMP8) and protein B (i.e. DMP9). The amino acid sequence of protein A is SEQ ID No. 1, and the amino acid sequence of protein B is SEQ ID No. 2. Also disclosed in the present invention are a method for constructing a plant haploid inducer line and a use thereof, and a plant haploid inducer line constructed by the method.

IPC Classes  ?

• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/84 - Ti-plasmids
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/54 - Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut

Abstract

Zea mays L.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells

Abstract

Provided is an artificial and synthetic material having biological activity, applied to agriculture, and containing an artificial hydrophilic protein, artificial hemoglobin, and polyhydroxyalkanoate (PHA) which are designed and synthesized in the present invention. The material can be used for preparing a micron-scale active artificial microcapsule that wraps agricultural microbial excellent strains having the characteristics of nitrogen fixation, phosphorus and potassium solubilization, disease and pest resistance, growth promotion, etc. to form a novel bacterial fertilizer, and can also be used in the agricultural field of preparing a novel crop seed active coating agent, etc.

IPC Classes  ?

• C07K 14/805 - HaemoglobinsMyoglobins
• C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
• C12N 15/12 - Genes encoding animal proteins
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• A01N 47/44 - GuanidineDerivatives thereof
• A01P 21/00 - Plant growth regulators
• C05F 11/08 - Organic fertilisers containing added bacterial cultures, mycelia or the like
• C05G 3/00 - Mixtures of one or more fertilisers with additives not having a specifically fertilising activity
• C05G 3/60 - Biocides or preservatives, e.g. disinfectants, pesticides or herbicidesPest repellants or attractants
• C05G 3/80 - Soil conditioners

Abstract

The present invention designs and constructs five artificial non-coding RNAs, respectively. The sequence of each artificial non-coding RNA has a complementary pairing region with a target mRNA, a binding site of an Hfq protein, and a Rho independent transcription terminator. The artificial non-coding RNAs can all be combined with an SD sequence of a target nitrogen fixation regulatory gene nifL mRNA 5'UTR, and can respectively prevent the mRNA from being combined with a ribosome RNA, so that the expression of a nitrogen fixation gene negative regulatory factor nifL is silenced.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12R 1/38 - Pseudomonas

Abstract

Provided are an expression vector of glyphosate-resistant genes GR79 and GAT, high glyphosate-resistant corn, and a detection method therefor. The codons of the GR79 gene and GAT gene which have high tolerance to glyphosate are optimized and new DNA sequences are synthesized. At the same time, a dual-gene plant expression vector pCGG is constructed. Results show that transgenic corn transformed with the plant expression vector has high resistance to target herbicide glyphosate. The transgenic corn GG2 is a transformation event having a significant glyphosate tolerance effect, and a left border flanking sequence and a right border flanking sequence thereof are obtained by means of a chromosome walking method. The border sequences at two ends can be used as specific detection sequences for the present transformation event. Primers designed on the basis of the two border sequences can specifically detect the transgenic event GG2 and be applied to the development of a detection kit.

IPC Classes  ?

• C12N 15/84 - Ti-plasmids
• C12N 15/54 - Transferases (2)
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

Provided are a vector system for genome methylation under a high temperature stress condition and an application thereof. The system is an expression vector comprising three expression boxes and can be applied to rice genome methylation.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 15/09 - Recombinant DNA-technology

Abstract

An amino acid sequence for improving protein orderliness and several key mutant sites of the sequence. Experimental results indicate that several recombinant proteins respectively obtained by performing gene replacement on these mutant sites can protect the activity of an enzyme under a stress condition, and can reduce enzyme aggregation and prevent the structure of the enzyme from being changed.

IPC Classes  ?

• C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
• C07K 14/00 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 9/96 - Stabilising an enzyme by forming an adduct or a compositionForming enzyme conjugates

Abstract

A functional module SyKerMb capable of efficiently degrading poultry feather waste and synthesizing a novel hemeprotein is artificially designed and constructed by using a synthetic biology method. A recombinant vector of the functional module is constructed, and assembled and expressed in chassis microorganism cell Escherichia coli. Experiments prove that engineering strain integrating the transformation function module has the biological synthesis capability of degrading animal feather waste and efficiently synthesizing an artificial hemeprotein, and can be applied in related industrial production of artificial protein preparations and future artificial food.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C07K 14/00 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin

Abstract

An artificial non-coding RNA module created by a synthetic biological method and a use thereof in construction of an artificial nitrogen fixation system. The RNA module enhances the stability of a nitrogenase coding gene nifHDK mRNA after transcription by interacting with the nifHDK mRNA, thereby improving the nitrogen fixation capability of chassis microorganisms. A fusion expression vector of the artificial RNA module is constructed and transferred into different chassis nitrogen fixation microorganisms. Experiments prove that under the condition of nitrogen fixation, the artificial RNA module can remarkably improve the activity of recombinant engineering strain nitrogenases.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12R 1/38 - Pseudomonas
• C12R 1/22 - Klebsiella
• C12R 1/065 - Azotobacter

Abstract

Provided in the present invention are a protein participating in the plant height and seed size of rice and an encoding gene thereof. Experiments prove that the protein can be used for cultivating dwarf transgenic rice and cultivating rice with a controllable seed size.

IPC Classes  ?

• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 5/04 - Plant cells or tissues

Abstract

Provided is an efficient artificial associative rhizosphere nitrogen fixation system, comprising: recombinant nitrogen fixation engineering bacteria which transform coding genes of a nitrogen fixation activator protein Neb and an ammonium transport protein amtR; and a recombinant plant which transforms a coding gene of an ammonium high-affinity protein Ham. Both components are functionally coupled on crop rhizospheres by means of a coating and inoculation technique.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• C12R 1/38 - Pseudomonas

Abstract

Provided is an application of the ZmSBP12 gene in the regulation and control of drought resistance, plant height and ear height of corn. After overexpressing the ZmSBP12 gene in corn, a corn mutant plant has characteristics such as increased drought resistance, reduced plant height and reduced ear height. Overexpression of ZmSBP12 leads to an increase in drought resistance and a reduction of plant height and ear height, indicating that the ZmSBP12 gene plays a vital role in characteristics such as drought resistance and plant type (plant height) corn. By increasing the expression abundance of the ZmSBP12 gene, the effects such as improving the drought resistance of the corn and reducing the plant height and ear height of the corn are achieved, and the gene may be applied to auxiliary breeding of new drought-resistant and lodging-resistant corn varieties and may also be applied to cultivation of excellent inbred lines and hybrids of the corn.

IPC Classes  ?

• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 5/04 - Stems
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

A ZmPHYCs mutant protein related to a flowering period of maize, an encoding gene, a recombinant vector, and use thereof. A CRISPR/Cas9 gene editing technology is used for mutating maize ZmPHYC1 and ZmPHYC2 genes together to create a novel early-flowering maize allele and delete an exogenous sequence of a CRISPR vector so as to obtain a strain with phenotype-stable inheritance. A functional deletion double mutant thereof exhibits an early-flowering phenotype under long-day conditions, so that it is determined that the ZmPHCYs mutant protein produced after the simultaneous mutation of the maize ZmPHYC1 and ZmPHYC2 genes have the effect of promoting the flowering of maize under long-day conditions. The present invention has great significance for regulating the flowering period of maize, can also be applied to maize inbred line improvement and cross breeding, and has advantages such as precision and high efficiency compared with conventional breeding.

IPC Classes  ?

• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize

Abstract

Provided are a key DNA sequence for regulating maize leaf angles and a mutant thereof. The polynucleotide sequence of the mutant is as shown in SEQ ID No. 1 and SEQ ID No. 2. The provided DNA sequence for regulating maize leaf angles and the mutant thereof can regulate the expression of ZmNAC16 gene in maize leaf pulvini, can therefore be applied to the regulation of maize leaf angles and improvement of the plant type, and can further be applied to the cultivation of new maize varieties. Also provided are specific detection primers for the DNA key sequence and mutation of the mutant thereof, and detection primers for detecting the expression of ZmNAC16 gene in maize. These detection primers can be used to directionally regulate the leaf angles of maize, and also have application potential for high dense planting and high-yield breeding of maize.

IPC Classes  ?

• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

Provided is a fungal operon, an exogeneous gene site-directed quantitative fixed-time expression system based on the fungal operon, and applications of the system in expressing heterogeneous genes. The system comprises the following sequences connected in series: a host target gene stop codon upstream fragment containing a stop codon, a fungal operon intergenic region, a target gene, and a DNA fragment of a host target gene stop codon downstream fragment. The system utilizes the fungal operon intergenic region to serially connect the target gene, inserts same to the downstream of a host cell expression gene stop codon by means of site-directed gene insertion, and utilizes a promoter of the target gene to complete the transcriptional expression of the target gene.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12R 1/865 - Saccharomyces cerevisiae

Abstract

Provided is a method for obtaining a gene module and route of rice sheath protoplast transformation, in particular: optimized conditions for transforming rice leaf sheath protoplast using a PEG-mediated method are provided, the conditions comprising the most suitable concentration ratio of protoplast to plasmid used for transformation, a PEG solution and amount that are used, the standing and culturing conditions once a rice leaf sheath protoplast suspension is added to the PEG solution, the pH values of related solutions, etc. It is confirmed by comparative experiments that the method of the present invention increases the transformation efficiency to 80%, and enables intelligent transformation components to function in 80% of plant cells.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 5/04 - Plant cells or tissues

Abstract

Provided is a gene dicX4 obtained from a metagenome of a soil sample and having a herbicide dicamba degradation function, and a recombinant vector containing the gene is constructed and expressed in a prokaryotic host cell Escherichia coli. Experiments have demonstrated that the gene has the herbicide dicamba degradation function, and can be used for breeding herbicide-resistant transgenic crops and for biologically repairing contaminated soil.

IPC Classes  ?

• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C07K 14/00 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy

Abstract

Provided are applications of a Zm5008 gene and a protein ZM5008 encoded thereby in regulation of a plant height and/or an internode distance of maize. Further provided is an application of an RNA inhibiting ZM5008 protein activity or inhibiting Zm5008 gene expression in regulation of a plant height and/or an internode distance of maize.

IPC Classes  ?

• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• A01H 5/04 - Stems

Abstract

Provided is a method for expressing a nitrogenase gene in an eukaryotic cell, comprising the following steps: 1) amplifying a nitrogenase gene fragment; 2) connecting the nitrogenase gene fragment to a virus transformation vector to construct a recombinant vector; 3) transfecting the eukaryotic cell by the recombinant vector constructed in step 2); and 4) screening a plant cell for expressing the nitrogenase gene. According to the present invention, the expression of the nitrogenase gene in a plant is implemented, thereby laying a foundation for autonomous nitrogen fixation of the plant.

IPC Classes  ?

• C12N 15/53 - Oxidoreductases (1)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/83 - Viral vectors, e.g. cauliflower mosaic virus

Abstract

Provided is a dwh gene separated from deinococcus radiodurans R1, the nucleotide sequence thereof being shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein thereof being shown in SEQ ID NO. 2. Further provided is an application of the dwh gene for cultivating antioxidant microorganisms. The expression of the dwh gene can improve the antioxidant capacity of E. coli.

IPC Classes  ?

• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12R 1/19 - Escherichia coli

Abstract

Aspergillus oryzae, with own signal peptides removed. The transglycosidase activity of the mutant is over 15% higher than that of wild types. Meanwhile, the present invention also discloses a DNA molecule which encodes the mutant, a recombinant expression vector containing the DNA molecule, and a host cell expressing the DNA molecule. In addition, the present invention also provides a method for preparing β-galactosidase mutant with high transglycosidase activity by using the recombinant expression vector and applications of the mutant, the DNA molecule, the recombinant expression vector and the host cell in preparation of β-galactosidase.

IPC Classes  ?

• C12N 9/38 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)
• C12N 9/14 - Hydrolases (3.)
• C12P 21/06 - Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• C07K 1/00 - General processes for the preparation of peptides

Abstract

Provided is amino acid optimization of a functional motif on IrrE protein of a deinococcus strain and a homologous protein thereof by using a manner of site mutation, in which second-site or fifth-site alanine in a functional domain motif 154LAELAR159 is mutated into serine.

IPC Classes  ?

• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12R 1/01 - Bacteria or actinomycetales

Abstract

Provided in the present invention are a glyphosate resistance gene-containing expression vector and the use thereof. The glyphosate resistance gene-containing expression vector is an annular vector which simultaneously expresses a glyphosate resistance gene 1 and a glyphosate resistance gene 2; a protein coded by the glyphosate resistance gene 1 is: a protein with glyphosate resistance obtained through the protein shown in sequence 1 or its amino acid sequence subjected to substitutions and/or deletions and/or additions by one or several amino acid residues; and a protein coded by the glyphosate resistance gene 2 is: a protein with glyphosate resistance obtained through the protein shown in sequence 2 or its amino acid sequence subjected to substitutions and/or deletions and/or additions by one or several amino acid residues.

IPC Classes  ?

• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/84 - Ti-plasmids
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants

Abstract

Provided is a β-galactosidase mutant with high transfer-glycoside activity, which is obtained on a basis of an amino acid sequence of β-galactosidase of aspergillus candidus and aspergillus oryzae with own signal peptide removed and by means of site-directed saturated mutation of a single site. The transfer-glycoside activity of the mutant is increased by more than 15% than a wild type. Also provided are a DNA molecule encoding the mutant, a recombinant expression vector comprising the DNA molecule and a host cell expressing the DNA molecule. Also provided are a method for preparing the β-galactosidase with high transfer-glycoside activity by using the recombinant expression vector, and uses of the mutant, the DNA molecule, the recombinant expression vector and the host cell in preparing the β-galactosidase.

IPC Classes  ?

• C12N 9/38 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase
• C12N 15/56 - Hydrolases (3) acting on glycosyl compounds (3.2), e.g. amylase, galactosidase, lysozyme
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12R 1/84 - Pichia

Abstract

Provided is a novel DNA polymerase with a continuous synthesis capability and salt tolerance. The DNA polymerase is a hybrid DNA polymerase which is prepared by inserting a thioredoxin binding structural domain from a T7 bacteriophage DNA polymerase into staphylococcus aureus DNA polymerase I (Sau), the amino acid sequence of the DNA polymerase is as shown in SEQ ID No.2, or an amino acid sequence which is formed by substitution or deletion or addition of one or more amino acids of the amino acid sequence is as shown in SEQ ID No.2 and has the same functions. Further provided are a gene encoding the protein, as well as a preparation method and a use of the protein.

IPC Classes  ?

• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
• C12P 21/00 - Preparation of peptides or proteins
• C07K 14/31 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)

Abstract

An EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) with high glyphosate resistance and a nucleotide sequence encoding the synthase are disclosed. The gene encoding the EPSP synthase has low homology with the reported EPSP synthase. A transgenic plant obtained by the expression of the gene in plant has an increased resistance to glyphosate after experimental confirmation.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12N 15/54 - Transferases (2)
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 1/00 - Processes for modifying genotypes
• A01P 21/00 - Plant growth regulators

Abstract

A nucleotide encoding sequence that can improve salt tolerance and drought tolerance of plant and a nucleotide encoding sequence artificially synthesized by using bias codons are firstly provided. The recombinant vectors comprising the genes are constructed, and then transform host cells of prokaryotic cells and eukaryotic cells, respectively. It turns out by experiments that after expressing the genes in the plants, the tolerances to salt and drought stress of the obtained transgenic plants are enhanced.

IPC Classes  ?

• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 5/14 - Plant cells
• C12P 19/30 - Nucleotides
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 1/00 - Processes for modifying genotypes
• C12R 1/00 - Microorganisms

Abstract

A method for expressing antigens of foot-and-mouth disease virus in insect in vivo by using recombinant baculovirus is disclosed, which comprises: cloning different genes combination of foot-and-mouth disease virus into baculovirus carrying vector to construct transfer vector, transfecting baculovirus with the transfer vector to proceed DNA recombination, and thus to get recombinant baculovirus, infecting insect host with the recombinant baculovirus, culturing the infected insect host to express the antigens of foot-and-mouth disease virus, and then collecting and purifying the expressed antigens. The preferred genes, baculovirus carrying vector, baculovirus and insect host are selected from P1-2A3C, ORF and VP1 genes of foot-and-mouth disease virus as shown in SEQ ID NO 1, 3 or 5, pVL1393, Bombyx mori Bm-NPV-ZJ8 and larvae or pupa of Bombyx mori, respectively.

IPC Classes  ?

• C12N 15/42 - Foot-and-mouth disease virus
• C12N 15/866 - Baculoviral vectors
• C12N 7/01 - Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
• C07K 14/09 - Foot-and-mouth disease virus
• A61K 39/135 - Foot-and-mouth disease virus
• A61P 31/12 - Antivirals
• C12R 1/03 - Actinomadura

Abstract

This patent belongs to the field of biotechnology. Highly efficient and controllable expression system for endogenous piggybacking exogeneous genes includes DNA fragments in the following order: DNA fragment upstream of endogenous target gene stop codon (including stop codon), intergenic gap of operon (IGG), gene of interest (GOI), DNA fragment downstream of endogenous target gene stop codon. It inserts the IGG linked GOI between the endogenous target gene and its terminator to form a bicistron by gene knock-in, leading to GOI expression controlled by the promoter of endogenous target gene. It would greatly simplify the operation, improve work efficiency, increase the controllability and stability of GOI expression, and reduce the requirement of multi-gene heterologous expression for characterized promoters. It would be a great synthetic biology tool for mining of novel natural products, yield improvement of valuable natural products and the development of unnatural compounds.

IPC Classes  ?

• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts