A sectionable tissue sample support structure including a gel compound formed into a self supporting geometric shape for retention and orientation of at least one tissue sample during a histopathology process including processing, embedding and microtome slicing of the tissue sample. A method of orienting, processing, embedding and microtome slicing a tissue sample using a gel compound preformed into a self supporting geometric shape. A combination including the sectionable tissue sample support structure and a package containing the sectionable tissue sample support structure.
A histologic tissue sample support device includes a tissue cassette a frame, and a lid. The tissue cassette has a recess including a body with at least one side wall and a bottom wall and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette is movably coupled to the frame. The lid and the tissue cassette are capable of moving from a first position to a second position with respect to the frame, and in the second position the bottom wall and at least a portion of the side wall extend beyond a bottom edge of the frame for sectioning in the microtome.
A method for preparing one or more biopsy tissue samples for histological examination includes positioning a tissue sample in a tissue cassette, closing a peripheral portion of a frame including a cassette lid, and moving the lid and the tissue cassette from a first position to a second position. The tissue cassette is coupled to a frame by a frame-cassette connector and moving the lid and the tissue cassette from the first position to the second position includes breaking the frame-cassette connector. The tissue cassette has a recess including at least one side wall and a bottom wall and is formed of a first material that can be successfully sectioned in a microtome. In the second position, the bottom wall and at least a portion of the side wall extend downwardly beyond a bottom edge of the frame for sectioning in the microtome.
A histologic tissue sample support device includes a tissue cassette, a frame, and a lid. The tissue cassette has a recess including at least one side wall and a bottom wall and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The lid is coupled to the frame and includes a platen configured to be received within the recess of the tissue cassette. The tissue cassette is movably coupled to the frame by a frame-cassette connector. The lid and the tissue cassette are capable of moving from a first position to a second position with respect to the frame, and in the second position the bottom wall and at least a portion of the side wall extend beyond a bottom edge of the frame for sectioning in a microtome.
A method for manufacturing a histologic tissue sample support device includes molding a tissue cassette, molding a frame, and coupling the tissue cassette to the frame by assembling a frame-cassette connector. The tissue cassette has a recess including at least one side wall and a bottom wall. The tissue cassette is formed of a first material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The frame includes a bottom edge and is formed of a second material different from the first material and more rigid than the first material. The frame-cassette connector includes a first retaining structure formed integrally with the frame extending through a second retaining structure formed integrally with the cassette. The frame is capable of being decoupled from the cassette by separating the frame-cassette connector.
A histologic tissue sample support device includes a tissue cassette, a frame, a lid, and a frame-cassette connector. The tissue cassette formed of a first material has a recess including a body with at least one side wall and a bottom wall and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette is movably coupled to the frame by a frame-cassette connector. The lid is coupled to the frame. The lid and the tissue cassette are capable of moving from a first position to a second position with respect to the frame, and in the second position the bottom wall and at least a portion of the side wall extend beyond a bottom edge of the frame for sectioning in a microtome.
A histologic tissue sample support device includes a tissue cassette a frame, and a lid. The tissue cassette has a recess including a body with at least one side wall and a bottom wall and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette is movably coupled to the frame. The lid is separably coupled to a peripheral portion of the frame. When the lid is separated from the peripheral portion, the lid and the tissue cassette are capable of moving from a first position to a second position with respect to the frame, and in the second position the bottom wall and at least a portion of the side wall extend beyond a bottom edge of the frame for sectioning in the microtome.
A histologic tissue sample support assembly (10) includes a tissue cassette (12), a frame (14), and a lid (18). The tissue cassette (12) has a recess (11) including a body with at least one side wall (16a, 16b, 16c, 16d) and a bottom wall (12b) and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette (12) is movably coupled to the frame (14) by a frame-cassette connector (64). The lid (18) is coupled to a peripheral portion of the frame (14) by a lid-frame connector (36). The lid (18) and the tissue cassette (12) are capable of moving from a first position to a second position with respect to the frame (14), and in the second position the bottom wall (12b) and at least a portion of the side wall (16a, 16b, 16c, 16d) extend beyond a bottom edge (14e) of the frame (14) for sectioning in a microtome.
A histologic tissue sample support assembly (10) includes a tissue cassette (12), a frame (14), and a lid (18). The tissue cassette (12) has a recess (11) including a body with at least one side wall (16a, 16b, 16c, 16d) and a bottom wall (12b) and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette (12) is movably coupled to the frame (14) by a frame-cassette connector (64). The lid (18) is coupled to a peripheral portion of the frame (14) by a lid-frame connector (36). The lid (18) and the tissue cassette (12) are capable of moving from a first position to a second position with respect to the frame (14), and in the second position the bottom wall (12b) and at least a portion of the side wall (16a, 16b, 16c, 16d) extend beyond a bottom edge (14e) of the frame (14) for sectioning in a microtome.
A histologic tissue sample support device includes a tissue cassette, a frame, and a lid. The tissue cassette has a recess including a body with at least one side wall and a bottom wall and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette is movably coupled to the frame by a frame-cassette connector. The lid is coupled to a peripheral portion of the frame by a lid-frame connector. The lid and the tissue cassette are capable of moving from a first position to a second position with respect to the frame, and in the second position the bottom wall and at least a portion of the side wall extend beyond a bottom edge of the frame for sectioning in a microtome.
A sectionable tissue sample support structure including a gel compound formed into a self supporting geometric shape for retention and orientation of at least one tissue sample during a histopathology process including processing, embedding and microtome slicing of the tissue sample. A method of orienting, processing, embedding and microtome slicing a tissue sample using a gel compound preformed into a self supporting geometric shape. A combination including the sectionable tissue sample support structure and a package containing the sectionable tissue sample support structure.
A histologic tissue sample support device includes a tissue cassette a frame, and a lid. The tissue cassette has a recess including a body with at least one side wall and a bottom wall and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette is movably coupled to the frame. The lid is separably coupled to a peripheral portion of the frame. When the lid is separated from the peripheral portion, the lid and the tissue cassette are capable of moving from a first position to a second position with respect to the frame, and in the second position the bottom wall and at least a portion of the side wall extend beyond a bottom edge of the frame for sectioning in the microtome.
A histologic tissue sample support device (200) includes a tissue cassette (112), a frame (14), and a lid (118"). The tissue cassette (112) has a recess (112b) including a body (112a) with at least one side wall and a bottom wall (112c) and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette (112) is movably coupled to the frame (14). The lid (118") is separably coupled to a peripheral portion (16) of the frame (14). When the lid (118") is separated from the peripheral portion (16), the lid (118") and the tissue cassette (112) are capable of moving from a first position to a second position with respect to the frame (14), and in the second position the bottom wall (112c) and at least a portion of the side wall extend beyond a bottom edge of the frame (14) for sectioning in the microtome.
A histologic tissue sample support device (200) includes a tissue cassette (112), a frame (14), and a lid (118"). The tissue cassette (112) has a recess (112b) including a body (112a) with at least one side wall and a bottom wall (112c) and is formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. The tissue cassette (112) is movably coupled to the frame (14). The lid (118") is separably coupled to a peripheral portion (16) of the frame (14). When the lid (118") is separated from the peripheral portion (16), the lid (118") and the tissue cassette (112) are capable of moving from a first position to a second position with respect to the frame (14), and in the second position the bottom wall (112c) and at least a portion of the side wall extend beyond a bottom edge of the frame (14) for sectioning in the microtome.
A sectionable tissue sample support structure (12) including a gel compound formed into a self supporting geometric shape for retention and orientation of at least one tissue sample (16) during a histopathology process including processing, embedding and microtome slicing of the tissue sample (1 6). A method of orienting, processing, embedding and microtome slicing a tissue sample (16) using a gel compound preformed into a self supporting geometric shape (12). A combination including the sectionable tissue sample support structure (12) and a package (40, 42) containing the sectionable tissue sample support structure (12).
A sectionable tissue sample support structure (12) including a gel compound formed into a self supporting geometric shape for retention and orientation of at least one tissue sample (16) during a histopathology process including processing, embedding and microtome slicing of the tissue sample (1 6). A method of orienting, processing, embedding and microtome slicing a tissue sample (16) using a gel compound preformed into a self supporting geometric shape (12). A combination including the sectionable tissue sample support structure (12) and a package (40, 42) containing the sectionable tissue sample support structure (12).
A sectionable tissue sample support structure including a gel compound formed into a self supporting geometric shape for retention and orientation of at least one tissue sample during a histopathology process including processing, embedding and microtome slicing of the tissue sample. A method of orienting, processing, embedding and microtome slicing a tissue sample using a gel compound preformed into a self supporting geometric shape. A combination including the sectionable tissue sample support structure and a package containing the sectionable tissue sample support structure.
Tissue orientation devices include a perforated tissue support with at least one perforated channel for receiving a tissue sample, and a plurality of tabs configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices include elongated legs coupled together for holding one or more biopsy tissue samples therebetween. Associated methods include using the cassettes and orientation devices to hold and orient tissue samples for processing, embedding and microtome sectioning.
A histologic tissue sample support device includes a tissue support formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. A resilient cellular material is coupled to the tissue support and is configured to engage and retain tissue in place during processing and embedding. The resilient cellular material is also capable of successful sectioning in the microtome and porous to allow infiltration of the solvents and chemicals used to fix, process and stain tissue, and of embedding material used to embed the tissue while the tissue is retained by the resilient cellular material.
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
20.
MICROTOME SECTIONABLE BIOPSY SUPPORT FOR ORIENTING TISSUE SAMPLES
Tissue orientation devices (10) include a perforated tissue support (12) with at least one perforated channel (30a-d) for receiving a tissue sample (100), and a plurality of tabs (18) configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices (200) include elongated legs (204, 206, 208, 210) coupled together for holding one or more biopsy tissue samples therebetween. Associated methods include using the cassettes (12) and ori-entation devices (200) to hold and orient tissue samples for processing, embedding and microtome sectioning.
Tissue orientation devices (10) include a perforated tissue support (12) with at least one perforated channel (30a-d) for receiving a tissue sample (100), and a plurality of tabs (18) configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices (200) include elongated legs (204, 206, 208, 210) coupled together for holding one or more biopsy tissue samples therebetween. Associated methods include using the cassettes (12) and orientation devices (200) to hold and orient tissue samples for processing, embedding and microtome sectioning.
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
22.
Microtome sectionable biopsy support for orienting tissue samples
Tissue orientation devices include a perforated tissue support with at least one perforated channel for receiving a tissue sample, and a plurality of tabs configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices include elongated legs coupled together for holding one or more biopsy tissue samples therebetween. Associated methods include using the cassettes and orientation devices to hold and orient tissue samples for processing, embedding and microtome sectioning.
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
23.
SYSTEMS AND METHODS FOR PROCESSING TISSUE SAMPLES FOR HISTOPATHOLOGY
A system for processing tissue biopsy samples during a histopathology process, including a tissue carrier (40) constructed to carry a tissue biopsy sample (54) during at least one step of the histopathology process, a database storing information associated with the tissue biopsy sample and associated with a tissue processing procedure, a machine-readable indicator (50) physically associated with the tissue carrier (40), the machine-readable indicator (50) including a machine- readable reference identified with the tissue biopsy sample (54), and an electronic control (48) operative to read the reference, access the information in the database using the reference, and implement at least a portion of the tissue processing procedure in accordance with the accessed information.
G06F 3/00 - Input arrangements for transferring data to be processed into a form capable of being handled by the computerOutput arrangements for transferring data from processing unit to output unit, e.g. interface arrangements
24.
BIOPSY SUPPORT WITH SECTIONABLE RESILIENT CELLULAR MATERIAL
A histologic tissue sample support device includes a tissue support (12) formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue (40). A resilient cellular material (20) is coupled to the tissue support (12) and is configured to engage and retain tissue (40) in place during processing and embedding. The resilient cellular material (20) is also capable of successful sectioning in the microtome and porous to allow infiltration of the solvents and chemicals used to fix, process and stain tissue, and of embedding material (50) used to embed the tissue (40) while the tissue (40) is retained by the resilient cellular material (20).
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
25.
Biopsy support with sectionable resilient cellular material
A histologic tissue sample support device includes a tissue support formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. A resilient cellular material is coupled to the tissue support and is configured to engage and retain tissue in place during processing and embedding. The resilient cellular material is also capable of successful sectioning in the microtome and porous to allow infiltration of the solvents and chemicals used to fix, process and stain tissue, and of embedding material used to embed the tissue while the tissue is retained by the resilient cellular material.
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
26.
Cassette for handling and holding tissue samples during processing, embedding and microtome procedures, and methods therefor
A cassette for holding a tissue sample includes a body having a bottom wall and a plurality of side walls extending upwardly with respect to the bottom wall to define an interior space for receiving the tissue sample. A lid is configured to be received in the interior space, and the cassette is sectionable in a microtome. Sensing elements are associated with the body or lid and configured to allow an automated sensing system to determine at least one characteristic of the cassette. A flange extends along upper portions of at least two of the side walls and includes a plurality of holes. Other features are included to more effectively and efficiently manufacture and use the cassette.
An automated machine for handling and embedding tissue samples contained on microtome sectionable supports. The machine includes an input member configured to hold a plurality of the microtome sectionable supports prior to a tissue embedding operation. An output member is configured to hold a plurality of the microtome sectionable supports after the tissue embedding operation. A cooling unit is configured to hold at least one of the microtome sectionable supports during the tissue embedding operation. A motorized carrier assembly is mounted for movement and configured to hold at least one of the microtome sectionable supports. The carrier assembly moves the support from the input member to the cooling unit and, finally, to the output member. A dispensing device dispenses an embedding material onto the microtome sectionable support and at least one tissue sample carried by the microtome sectionable support during the embedding operation.
G01N 21/00 - Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
28.
Cassette and embedding assembly for handling and holding tissue samples during processing, embedding and microtome procedures, staging devices therefore, and methods therefor
The disclosure relates to a cassette, frame and mold for holding a tissue sample during an embedding and mircotoming process, and related methods. The cassette is sectionable in a microtome and includes a body with a bottom wall and a plurality of side walls. First and second side walls are generally V-shaped to present an apex of the “V” to the microtome blade. A lid of the cassette is stiffer than the bottom wall of the cassette to assist with positioning the tissue sample. The side walls of the cassette are perforated so as to significantly reduce the amount of cassette material that must be cut by the microtome blade. In one embodiment, to additionally reduce blade wear the ribs on one side wall are offset lengthwise relative to the ribs on an opposite side wall. An upper flange of the cassette includes depressions configured to register with detents in the frame.
A cassette (10) for holding a tissue sample includes a body having a bottom wall (28) and a plurality of side walls (26) extending upwardly with respect to the bottom wall to define an interior space for receiving the tissue sample. A lid (24) is configured to be received in the interior space, and the cassette (10) is sectionable in a microtome. Sensing elements (12) are associated with the body (16) or lid (24) and configured to allow an automated sensing system to determine at least one characteristic of the cassette (10). A flange (14) extends along upper portions of at least two of the side walls (26) and includes a plurality of holes (12, 20). Other features are included to more effectively and efficiently manufacture and use the cassette (10).
A cassette (10) for holding a tissue sample includes a body having a bottom wall (28) and a plurality of side walls (26) extending upwardly with respect to the bottom wall to define an interior space for receiving the tissue sample. A lid (24) is configured to be received in the interior space, and the cassette (10) is sectionable in a microtome. Sensing elements (12) are associated with the body (16) or lid (24) and configured to allow an automated sensing system to determine at least one characteristic of the cassette (10). A flange (14) extends along upper portions of at least two of the side walls (26) and includes a plurality of holes (12, 20). Other features are included to more effectively and efficiently manufacture and use the cassette (10).
patents
