A method for performing fluorescence measurement on a sample, including: illuminating the sample using a light source, acquiring at least one fluorescence image of the illuminated sample, and processing the fluorescence image to determine a fluorescence intensity of the sample; characterized in that processing the fluorescence image to determine a fluorescence intensity of the sample includes: extracting, from the fluorescence image, a region of interest (ROI) free from artefacts, by application of a trained model, and determining the fluorescence intensity of the sample from the extracted region of interest.
The invention relates to a computer-implemented method for characterising a microbial strain, which method comprises the following steps: - obtaining a spectral image of a colony, representing a colony of a microbial strain, the spectral image of the colony comprising a plurality of pixels, each pixel comprising a plurality of spectral channels; - filtering the spectral image of the colony in order to obtain a "test image", each pixel in the test image being dependent on an average of a packet of neighbouring pixels in the image of the colony, each packet comprising M
G06V 10/34 - Smoothing or thinning of the patternMorphological operationsSkeletonisation
G06V 10/58 - Extraction of image or video features relating to hyperspectral data
G06V 10/80 - Fusion, i.e. combining data from various sources at the sensor level, preprocessing level, feature extraction level or classification level
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
3.
SYSTEM AND METHOD FOR IDENTIFYING ANALYTES IN ASSAY USING NORMALIZED TM VALUES
Techniques are provided for generating an array-specific range of Tm values to be used for calling a sample in a given array positive or negative for a target nucleic acid sequence. A sample well in an array is provided with a control sample containing a control nucleic acid sequence. The control sample is amplified by thermal cycling the sample well. A Tm value for the control sample is identified and compared to an expected Tm value for the control nucleic acid sequence to calculate a relationship between the identified control Tm value and the expected control Tm value. By applying this relationship to an expected Tm value for a target nucleic acid sequence, an array-specific range of Tm values for the target nucleic acid sequence is generated and can be used for calling an experimental sample in the same array positive or negative for the target nucleic acid sequence.
The invention relates to an in vitro or ex vivo determination method for determining the risk of death of a patient infected by a respiratory virus, said method comprising the steps of measuring, in a biological sample from said patient, the expression level of the ADGRE3 gene, and of comparing the expression level thus measured or a value derived from this quantity with a predetermined reference value. The method thus makes it possible to conclude that there is an increased risk of death of the patient when an underexpression of the ADGRE3 gene is statistically demonstrated from the biological sample. Advantageously, the measurement of the expression level of ADGRE3 can be supplemented by the measurement of one or more additional genes such as C3AR1, CD177, OAS2, CIITA, IL-10, IL1R2, CD74, TDRD9) and combinations thereof. The invention also relates to kits for measuring the expression of ADGRE3 and optionally of one or more additional genes.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
5.
METHOD FOR DETECTING THE PRESENCE OF A BIOLOGICAL SPECIES OF INTEREST BY ITERATIVE REAL-TIME SEQUENCING
A metagenomic analysis of a sample for the purpose of detecting the presence of a species of interest in the sample is carried out iteratively. During each iteration, sequences corresponding to each species of interest are identified and counted. The iterations stop when the presence of a species of interest is confirmed or when a maximum number of iterations have been carried out. The detection of a species of interest can be followed by a more precise characterization of the genome of said species of interest. The characterization implements supplementary iterations.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6809 - Methods for determination or identification of nucleic acids involving differential detection
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
37 - Construction and mining; installation and repair services
39 - Transport, packaging, storage and travel services
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
Chemical reagents and biological preparations for diagnosis,
other than for medical or veterinary use; reagents and
culture media for the monitoring, detection, diagnosis and
analysis of microorganisms in industrial, agri-food,
cosmetic and pharmaceutical products and in the production
environments of these products, namely water, air and work
surfaces. Reagents and culture media for pharmaceutical, medical and
veterinary use enabling the monitoring, detection, diagnosis
and analysis of microorganisms. Scientific apparatus and instruments for diagnosis (not for
medical use) and for monitoring and detecting microorganisms
in industrial, agri-food, cosmetic and pharmaceutical
products and in the production environments of these
products, namely water, air and work surfaces; petri dishes. Diagnostic apparatus and instruments for medical and
veterinary use; petri dishes for medical and veterinary use;
containers for collecting medical and veterinary waste;
containers for collecting petri dishes contaminated by
microorganisms. Disinfecting, sanitizing, sterilizing and cleaning petri
dishes and apparatus and instruments for diagnosing
microorganisms; providing information, advice and assistance
with respect to the disinfection, sanitation, sterilization
and cleaning of petri dishes and apparatus and instruments
for diagnosing microorganisms. Collecting and transporting waste, namely petri dishes and
apparatus and instruments for diagnosing microorganisms;
provision of information, advice and assistance with respect
to the collection and transport of waste, namely petri
dishes and apparatus and instruments for diagnosing
microorganisms. Sorting, recycling, treating and processing waste, namely
petri dishes and apparatus and instruments for diagnosing
microorganisms; provision of information, advice and
assistance with respect to sorting, recycling, treating and
processing waste, namely petri dishes and apparatus and
instruments for diagnosing microorganisms.
8.
METHOD FOR MANUFACTURING A BIOLOGICAL ANALYSIS CARD INVOLVING A HEATING BLOCK
The invention relates to a method for manufacturing a biological analysis card (1) comprising:—a step a) of providing a base (2) formed of:—at least two films (3) placed one on top of the other;—a receiving space (4) formed between the two films (3); —ducts (5) leading to the receiving space (4);—a step b) of placing a plate (9) in the receiving space (4), the plate (9) comprising:—a first face (10a) and a second face connected by an edge (11) having a profile;—a plurality of wells (12) opening onto at least the first face (10a) or the second face;—a plurality of channels (13) fluidly connecting the wells (12);—a welding step c) for welding the two films (3) in the receiving space (4) to the plate (9) and a film (3) to segments of the edge (11) of the plate (9) by applying a heating block to the film (3) against the edge (11) on the side of the first face (10a).
The invention relates to an in vitro or ex vivo method for determining the risk of death for a subject infected with a respiratory virus, said method comprising the steps of measuring, in a biological sample from said subject, the expression level of the OAS2 gene, and comparing the expression level thus measured or a value derived from this amount to a predetermined reference value. The method thus makes it possible to conclude that there is an increased risk of death for the subject when a sub-expression of the OAS2 gene is statistically demonstrated from the biological sample. Advantageously, the measurement of the expression level of OAS2 can be supplemented by a measurement of one or more additional genes such as C3ARI, CD177, ADGRE3, CIITA, IL-10, ILIR2, CD74, TDRD9 and combinations thereof. Kits for measuring the expression of OAS2 and optionally one or more additional genes are also disclosed.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
10.
DETERMINING THE RISK OF DEATH OF A SUBJECT INFECTED WITH A RESPIRATORY VIRUS BY MEASURING THE EXPRESSION LEVEL OF THE CD74 GENE
The invention relates to an in vitro or ex vivo method for determining the risk of death in a subject infected with a respiratory virus, for example SARS-CoV-2, comprising a measurement, in a biological sample of said subject, of the level of expression of the CD74 gene; the invention also relates to associated kits.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
11.
METHODS AND COMPOSITIONS FOR ASSESSING AND TREATING PERSISTENT ACUTE KIDNEY INJURY BASED ON C-C MOTIF CHEMOKINE LIGAND 14 MEASUREMENT
The present invention relates to methods and compositions for assessing a risk for developing persistent acute kidney injury and methods of treating a subject based on the assessment. In particular, disclosed herein are methods and compositions for detecting C—C motif chemokine ligand 14 (CCL14) for predicting the risk of persistent acute kidney injury based on changes in the level of CCL14 in two or more measurements of CCL14 in a subject and/or based on two cutoff levels of CCL14.
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) (France)
Inventor
Courbon, Benoît
Faure, Nicolas
Sapay, Nicolas
Abstract
The disclosed method for classifying microorganisms contained in a sample comprises: preparing a slide of the sample; acquiring at least one digital image of the slide; and implementing, with a computer, a model for predicting the class of the microorganisms depending on the acquired image. According to the invention, said image is subdivided into sub-images and each sub-image is sub-divided into patches, and: A. for each patch, a microorganism feature extractor is applied, the feature extractor forming a convolutional part of a first convolutional neural network trained on patches annotated individually with at least one class; B. for each sub-image, a second neural network connected to the extractor is applied, the second neural network comprising an upstream pooling layer and one or more downstream layers comprising a layer for predicting at least one class, and being trained on training sub-images globally; and C. for the acquired image: F.a. calculating a feature vector calculated for the sub-images; F.b. applying a model for predicting at least one class for the microorganisms.
G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersectionsConnectivity analysis, e.g. of connected components
G06V 10/50 - Extraction of image or video features by performing operations within image blocksExtraction of image or video features by using histograms, e.g. histogram of oriented gradients [HoG]Extraction of image or video features by summing image-intensity valuesProjection analysis
G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
13.
PTX3 BIOMARKER FOR REDUCING ANTIBIOTIC TREATMENT IN NEWBORNS
The invention relates to an in vitro method for determining whether a newborn patient has late-onset neonatal sepsis, which comprises determining the PTX-3 protein concentration in a biological sample previously obtained from said patient.
The invention relates to a gelled reaction medium for the detection, identification, enumeration and/or isolation of at least one target microorganism in a simple that may contain same, comprising at least one binding partner specific to a component of a target microorganism or of a component derived from said microorganism, coupled to at least one nanoparticle to form at least one binding conjugate.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
A method for detecting an immune cellular response including the steps of: (a) suctioning a defined amount of the sample by an automated suction/discharge device, (b) discharging this amount, using the device, into container R, containing, beforehand, at least one stimulant for secretion of the molecule by the at least one cell, or else not containing such a stimulant, (c) when the container does not contain the stimulant beforehand, introducing into container R the at least one secretion stimulant contained in container E, using the device, and (d) allowing the sample and stimulant to incubate, forming a mixture, by the at least one cell (i) in container R or (ii) in another container S after suctioning and discharge of the mixture using the device, and (e) detecting the immune cellular response, the detection of the immune cellular response indicates the presence of an immune cellular response specific for the stimulant.
The present invention relates to a method for quickly determining the immune status of an individual. The method comprises the following steps: taking a volume of a whole blood sample from the individual; stimulating the whole blood sample by incubating the latter with a quantity of phytohemagglutinin (PHA) at a temperature between 35° C. and 39° C. for a minimum of 3 to 3.5 hours; evaluating the level of interferon-gamma production induced by this incubation/stimulation, the evaluated level subsequently providing an indication of the individual's immune status.
The invention concerns a method of assessing the risk of death of a patient, comprising the steps of: A1. Measuring a first value V1 reflecting the level of expression of CX3CR1 gene in a biological sample obtained from said patient, B1. Measuring a second value V2 reflecting the level of expression of CDK1 gene in a biological sample obtained from said patient.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
G01N 33/52 - Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
37 - Construction and mining; installation and repair services
39 - Transport, packaging, storage and travel services
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
Chemical reagents and biological preparations for diagnosis, Not for medical or veterinary use; Reagents and culture media for the monitoring, detection, diagnosis and analysis of microorganisms in industrial, agri-food, cosmetic and pharmaceutical preparations and in the environment in which these preparations are produced, namely water, air and work surfaces. Reagents and culture media enabling the monitoring, detection, diagnosis and analysis of microorganisms for medical, pharmaceutical and veterinary purposes. Scientific apparatus and instruments for the diagnosis (not for medical purposes) and for the monitoring and detection of microorganisms in industrial, agri-food, cosmetic and pharmaceutical preparations and in the environment in which these preparations are produced, namely water, air and work surfaces; Petri dishes. Diagnostic apparatus and instruments for medical and veterinary use; Petri dishes For medical and veterinary purposes; Containers for the collection of medical and veterinary waste; Containers for the collection of Petri dishes contaminated with microorganisms. Disinfection, sanitisation, sterilisation and cleaning of Petri dishes and apparatus and instruments for the diagnosis of microorganisms; Providing of information, advice and assistance relating to the disinfection, sanitisation, sterilisation and cleaning of Petri dishes and of apparatus and instruments for the diagnosis of microorganisms. Collection and transport of waste, namely Collection and transport of Petri dishes and of apparatus and instruments for the diagnosis of microorganisms; Information and consultancy and advisory services Relating to the collection and transport of waste, namely Petri dishes and apparatus and instruments for the diagnosis of microorganisms. Sorting, recycling, treatment and processing of waste, namely Petri dishes and apparatus and instruments for the diagnosis of microorganisms; Information, consultancy and advisory services Relating to the sorting, recycling, treatment and processing of waste, namely namely Petri dishes and apparatus and instruments for the diagnosis of microorganisms.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
37 - Construction and mining; installation and repair services
39 - Transport, packaging, storage and travel services
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
Chemical reagents and biological preparations for diagnosis, other than for medical or veterinary use; reagents and culture media for the monitoring, detection, diagnosis and analysis of microorganisms in industrial, agri-food, cosmetic and pharmaceutical products and in the production environments of these products, namely water, air and work surfaces. Reagents and culture media for pharmaceutical, medical and veterinary use enabling the monitoring, detection, diagnosis and analysis of microorganisms. Scientific apparatus and instruments for diagnosis (not for medical use) and for monitoring and detecting microorganisms in industrial, agri-food, cosmetic and pharmaceutical products and in the production environments of these products, namely water, air and work surfaces; petri dishes. Diagnostic apparatus and instruments for medical and veterinary use; petri dishes for medical and veterinary use; containers for collecting medical and veterinary waste; containers for collecting petri dishes contaminated by microorganisms. Disinfecting, sanitizing, sterilizing and cleaning petri dishes and apparatus and instruments for diagnosing microorganisms; providing information, advice and assistance with respect to the disinfection, sanitation, sterilization and cleaning of petri dishes and apparatus and instruments for diagnosing microorganisms. Collecting and transporting waste, namely petri dishes and apparatus and instruments for diagnosing microorganisms; provision of information, advice and assistance with respect to the collection and transport of waste, namely petri dishes and apparatus and instruments for diagnosing microorganisms. Sorting, recycling, treating and processing waste, namely petri dishes and apparatus and instruments for diagnosing microorganisms; provision of information, advice and assistance with respect to sorting, recycling, treating and processing waste, namely petri dishes and apparatus and instruments for diagnosing microorganisms.
23.
DETECTION OF A GENOMIC SEQUENCE IN A MICROORGANISM GENOME BY WHOLE GENOME SEQUENCING
A method for detecting target genomic sequences at predetermined positions in a sequenced genome of a microbial organism in the form of reads. The method comprises computing a local sequencing depth of the genome in a neighborhood of the position of a target genomic sequence, the computing including a) detecting in the reads a set of digital genomic sequences from at least one reference genome of the microbial organism, b) generating of third set of digital genomic sequences comprising reads and digital genomic sequences of the second set belonging to the neighborhood c) counting the number of copies of each digital genomic sequences of the third set of digital genomic sequences, and d) computing the local sequencing depth as being equal to the maximum of the counted numbers of copies.
G16B 30/00 - ICT specially adapted for sequence analysis involving nucleotides or amino acids
G06F 30/27 - Design optimisation, verification or simulation using machine learning, e.g. artificial intelligence, neural networks, support vector machines [SVM] or training a model
12344, are as defined in claim 1, including their salts. The invention concerns also a detection probe PR labelled with at least one molecule of a compound FC according to the invention, comprising an oligonucleotide covalently coupled on the reactive group RG of said compound FC, the methods and kits using them.
C07D 417/14 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group containing three or more hetero rings
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
25.
METHOD FOR DETECTING VIABLE MICROORGANISMS WHICH ARE POTENTIALLY PRESENT IN A SAMPLE OF CELLULAR PRODUCTS
The present invention relates to a method for isolating viable microorganisms which are potentially present in a sample comprising 106 to 108 immune cells or progenitor cells thereof, the method comprising the following steps: - bringing the sample into contact with: • a lysing composition comprising o a lysis buffer comprising a non-ionic detergent at a concentration of between 0.004% and 0.050% of the reaction volume o and/or a lysis solution comprising a saponin at a concentration of between 0.03% and 4% of the reaction volume; • an endonuclease for digesting the nucleic acids released by the action of the lysing composition; • an endopeptidase acting at a pH of between 7 and 8; - separating the viable microorganisms which are potentially present in the sample by filtering the sample through a filter, the pores of which have a diameter of between 0.30 and 0.50 μm. The present invention also relates to the associated solid-phase cytometry detection method.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.
The present invention relates to in vitro or ex vivo methods and kits for determining the viral or bacterial nature of an infection on the basis of a biological sample of a patient by identifying the change in the level of expression of a plurality of biomarkers. In particular, the method includes the steps of (a) measuring the expression of at least one viral target gene and of at least one bacterial target gene chosen from among OLAH, FAM20A, IL1R2, MMP8, RETN and SLC1A2; (b) comparing the expressions measured in step (a) with reference expression values for these target genes; and (c) on the basis of the results of the comparison, concluding whether the infection is viral or bacterial in nature.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6809 - Methods for determination or identification of nucleic acids involving differential detection
CandidaC. aurisC. albicans, C. glabrata, C.tropicalis, C. parapsilosis and C. krusei.CandidaCandida genus; and - an α-glucosidase inducer and optionally an α-glucosidase repressor; - an acid phosphatase inducer and a phosphatase repressor; - an N-acetyl-β-glucosaminidase inducer; and - detecting any α-glucosidase, acid phosphatase and/or N-acetyl-β-glucosaminidase activities expressed by the yeasts; and - identifying the species to which the yeasts belong on the basis of the α-glucosidase, acid phosphatase and/or N-acetyl-β-glucosaminidase activities actually expressed by the yeasts.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/25 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving enzymes not classifiable in groups
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
30.
MODULE FOR MAGNETICALLY EXTRACTING COMPONENTS FROM A SAMPLE
A system for extracting components from a biological sample, the system including at least one extraction platform, a magnetic extraction module including a magnetic portion and a securing portion for securing the module to the extraction platform, the magnetic portion including at least one magnet that is fixedly mounted relative to the securing portion, the extraction platform including a pipetting support that is configured to co-operate with a pipetting device, characterised in that the magnetic portion of the magnetic extraction module has at least one position of use in which the magnetic portion is fixed at least relative to the pipetting support.
A method for in vitro or ex vivo assessment of the risk of complications in a patient admitted to a healthcare facility includes measuring the viral load of at least one torque teno virus (TTV) in a biological sample of the patient, wherein the patient is not undergoing immunosuppressive treatment.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Computer software and program dedicated to microbiology
laboratory management. Software as a Service (SaaS) dedicated to microbiology
laboratory management.
33.
ANALYSIS CARD FOR ANALYSING A BIOLOGICAL SAMPLE, AND PRODUCTION AND QUALITY CONTROL METHOD
An analysis card for analysing a biological sample by means of an in-vitro diagnostic instrument, the analysis card including a plurality of wells formed in a board, each well containing at least one reagent, the analysis card including a channel for conveying a liquid sample to the wells, characterised in that each well forms, in the board, an internal space defined by a lateral surface, the lateral surface including at least one wall, and in that each well has the at least one reagent deposited only on its lateral surface.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
Goods & Services
Culture media for the detection and identification of pathogens in food, pharmaceutical and cosmetic products and in the production environment of these products (water, air, work surfaces). culture media for use in the following fields: medical diagnosis and Veterinary diagnosis. Petri dishes.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
Goods & Services
Culture media for detection and identification of pathogens in agri-food, pharmaceutical, cosmetic products and in the production environment of these products (water, air, work surfaces). Culture media for medical and veterinary diagnosis. Petri dishes.
36.
SYSTEMS AND METHODS FOR POINT OF USE EVACUATION OF AN ARRAY
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) (France)
Inventor
Brault, Dylan
Fournier, Corinne
Thomas, Olivier
Faure, Nicolas
Abstract
The invention relates to a method for characterising microorganisms which comprises: A. for each wavelength of a predetermined set of wavelengths comprising at least one wavelength, acquiring a holographic digital image, generating a focused image by computer means, each pixel of which image comprises an amplitude value and a phase value, and segmenting the focused image so as to extract portions corresponding to microorganisms; and B. characterising the microorganisms by computer means, according to the focused image portions. According to the invention, prior to the characterisation or prior to the segmentation or prior to the generation of the focused image, the method comprises correcting optical aberrations of the acquisition device by computer means, and the characterisation of the microorganisms comprises applying a digital model to these portions having as descriptors the amplitude and/or phase values of each wavelength of the predetermined set of wavelengths.
G03H 1/00 - Holographic processes or apparatus using light, infrared, or ultraviolet waves for obtaining holograms or for obtaining an image from themDetails peculiar thereto
G03H 1/04 - Processes or apparatus for producing holograms
G03H 1/26 - Processes or apparatus specially adapted to produce multiple holograms or to obtain images from them, e.g. multicolour technique
G01N 15/1433 - Signal processing using image recognition
G06V 10/143 - Sensing or illuminating at different wavelengths
G06V 10/22 - Image preprocessing by selection of a specific region containing or referencing a patternLocating or processing of specific regions to guide the detection or recognition
G06V 10/24 - Aligning, centring, orientation detection or correction of the image
G06V 10/26 - Segmentation of patterns in the image fieldCutting or merging of image elements to establish the pattern region, e.g. clustering-based techniquesDetection of occlusion
G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersectionsConnectivity analysis, e.g. of connected components
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific apparatus, instruments and devices, not for
medical use, namely, applicators for plates for collection
and detection of contaminants in industrial, agri-food,
cosmetic and pharmaceutical products and in the production
environment for these products, namely, water, air, and
surfaces environments, and for in-vitro diagnosis.
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Computer software and programs for use in microbiology laboratory management. Software as a service (SaaS) for use in microbiology laboratory management.
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Downloadable computer software and downloadable computer program for microbiology laboratory management Software as a Service (SaaS) services featuring software for microbiology laboratory management
41.
METHOD AND SYSTEM FOR IDENTIFYING MICRO-ORGANISMS TAKING COLONY FORM
The invention relates to a method for identifying a microbial strain taking the form of a colony, comprising acquiring a set of at least three spectral channels of the colony contained in the visible and infrared spectrum; and making, by means of a computer, a prediction of the species depending on the acquired spectral channels. The three channels of said set that contribute the most to the performance of the prediction are selected from a first spectral band [730-780] nm and from a second spectral band [550-650] nm.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
G01N 21/359 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
The present document relates to an optical device configured to measure fluorescence and absorbance in an immunoassay and in wet chemistry, comprising: - an illuminator channel (26) comprising at least one excitation source (28), - a detector channel (34) comprising at least one sensor (36), - a cuvette (130) containing a sample (132) between the illuminator channel (26) and the detector channel (34), - a mixer (38) of optical light between the illuminator channel (26) and the cuvette (130) and a beam splitter (40) between the cuvette (130) and the detector channel (34).
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 21/25 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
Escherichia ColiEscherichia Coli (STEC) which may be present in a sample comprising enterobacteria, comprising the following steps: - performing lysis of the sample, enabling lysis of the STECs in order to obtain a solution comprising the nucleic acids thereof; - bringing the solution of nucleic acids into contact with primers, making it possible to amplify at least the stx1 and/or stx2 gene or gene fragment; - if at least one of the stx1 and/or stx2 genes or gene fragments is amplified, part of the sample is deposited on an agar reaction medium comprising ■ at least one toxin inducer, ■ at least one agglutinating conjugate formed by at least one binding partner specific to the STX1 protein and/or at least one binding partner specific to the STX2 protein, which binding partner(s) is (are) coupled to a nanoparticle; - detecting and confirming the presence of at least one STEC by the appearance of a halo on the agar around the STEC.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
The invention relates to a method for detecting colonies of microorganisms in a biological sample arranged in a solid culture medium, the method being implemented by a detection system comprising at least one incubator, an analysis unit, and at least one automated image capture system, the method comprising a first phase of detecting and counting objects, in the field of view of the image capture system, exhibiting growth, and a second phase applied only to those samples deemed to be negative in the first phase of the method, the second phase being based on anomaly detection applied to at least the last image acquired in the incubation sequence.
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
48.
MANUFACTURE OF POLYMERASE CHAIN AMPLIFICATION KITS OPTIMIZING AN AMPLIFICATION HYBRIDIZATION PHASE
Disclosed is a method for digitally simulating a polymerase chain amplification of at least one target, comprising the simulation of a plurality of successive cycles of the amplification, each cycle comprising: a hybridization phase; an elongation phase; then a denaturation phase; wherein the hybridization phase comprises, for each cycle: obtaining, for the cycle, an initial value of a vector of concentrations of a plurality of nucleic acid molecules in the form of single strands; initializing a matrix of concentrations of duplexes formed by the hybridization of the plurality of nucleic acid molecules in the form of single strands; calculating the change in the matrix of concentrations during the hybridization phase by applying, to the matrix of concentrations, a matrix differential equation representing the kinetics of the duplex formation according to the concentrations of the plurality of nucleic acid molecules.
Disclosed is a method for digitally simulating a polymerase chain amplification of at least one target, comprising the simulation of a plurality of successive cycles of the amplification, each cycle comprising, sequentially: a hybridization phase; an elongation phase; then a denaturation phase; wherein, for each cycle: the hybridization phase comprises: obtaining, for the cycle, an initial value of a vector of concentrations of a plurality of nucleic acid molecules in the form of single strands; calculating a matrix of concentrations of partial duplexes formed by the hybridization of the plurality of nucleic acid molecules in the form of single strands; the denaturation phase comprises: multiplying a vector of the concentration of elongated duplexes resulting from the application of the elongation phase to the concentrations of duplexes, by an elongated duplex denaturation tensor.
A method for determining in vitro or ex vivo the immune status of an individual, preferably a patient, including a step of detecting and/or quantifying the expression of one or more HERV/MaLR sequences in a biological sample of the individual. Also relates to the tools for implementing the method and to the uses thereof.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
51.
METHOD FOR DETERMINING THE SUSCEPTIBILITY OF A MICROORGANISM TO AN ANTIMICROBIAL AGENT
The present invention relates to a method for predicting the susceptibility of a microbial strain to an antimicrobial agent, the method being characterized in that it comprises implementation, by data-processing means (4) of a client (2), of steps of: (a) obtaining a hyperspectral image between 390 nm and 900 nm representing at least one colony of said strain in a sample devoid of antimicrobial agent (22); (b) determining a spectrum of the colony based on pixels of said hyperspectral image corresponding to said colony, “test spectrum” below; (c) comparing said test spectrum with microbial classes of a database of predetermined data, “reference microbial class” below, said classes corresponding to a taxonomic level lower than species and being learnt using at least one hyperspectral spectrum of a microbial strain, the database containing, for each reference microbial class, the susceptibility to the microbial agent of the reference microbial class; (d) determining the susceptibility of the microbial strain to the microbial agent to be the susceptibility associated with the reference microbial class closest to the test hyperspectral spectrum.
The invention relates to a ready-to-use calibration composition for a mass spectrometer, comprising whole E. coli bacteria suspended in a liquid which is based on alcohol (s) and/or acetonitrile and is sanitizing, volatile and does not denature bacterial proteins.
The invention relates to a microbiological typing method, which comprises providing: a database of genetic profiles of microbial strains; and a phylogenetic tree of assignments of the genetic profiles to positions in the phylogenetic tree, and of frequencies of variation of the profiles in the tree. The typing of a microorganism comprises: measuring the genetic profile of the microorganism; determining a probability of variation of each genetic profile in the database with respect to the measured genetic profile, the probability of variation being calculated as a function of the frequencies of variation; and determining that the microorganism belongs to a taxon of the tree if at least the probability of variation for the taxon is greater than a threshold. According to the invention, the phylogenetic tree is constructed from a core genome of the microorganism species, and the predefined set of genetic markers is chosen from an accessory genome of the microorganism species.
The invention relates to a microbiological typing method, which comprises providing a database of genetic profiles of microbial strains, a phylogenetic tree, assignments of the genetic profiles to positions in the phylogenetic tree, and frequencies of variation of the profiles in the tree. The typing of a microorganism comprises measuring the genetic profile of the microorganism, determining probabilities of variation of the genetic profiles belonging to the groups with respect to the measured genetic profile, the probabilities of variation being calculated as a function of the frequencies of variation at a predefined level of the phylogenetic tree and of the belonging of the microorganism to one of the microbial groups if an identification performance criterion is reached for the group, the criterion being reached if at least one probability of variation for the group is greater than a first predefined threshold.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Chemical preparations for industry and research; chemical
preparations for scientific use; chemical reagents and
biological preparations for diagnosis, other than for
medical use; reagents and culture media for the control,
detection, diagnosis and analysis of contaminating
microorganisms and substances in industrial, agri-food,
cosmetic and pharmaceutical products; reagents and media
enabling the control, diagnosis and analysis of
contaminating microorganisms and substances for detecting
biological threats and monitoring epidemics and pandemics. Reagents and culture media for medical and veterinary
diagnosis; laboratory reagents for use in clinical, medical
or veterinary laboratories; chemical, biochemical and
biological preparations for use in the medical and
veterinary fields; reagents and culture media for medical
and veterinary use enabling the control, diagnosis and
analysis of contaminating microorganisms and substances for
detecting biological threats and monitoring epidemics and
pandemics. Diagnostic apparatus and instruments, not for medical use;
scientific analysis and diagnostic apparatus and
instruments, other than for medical use; laboratory
instruments; Scientific apparatus and instruments for the
monitoring, detection, diagnosis and analysis of
contaminating substances in industrial, agri-food, cosmetic,
pharmaceutical and veterinary products; scientific apparatus
and instruments enabling the control, diagnosis and analysis
of contaminating microorganisms and substances for detecting
biological threats and monitoring epidemics and pandemics;
software for analyzing, processing and interpreting medical
and veterinary test results in the field of in vitro
diagnosis. Apparatus and instruments for medical and veterinary
diagnostic purposes; apparatus and instruments for medical
and veterinary use enabling the control, diagnosis and
analysis of contaminating microorganisms and substances for
detecting biological threats and monitoring epidemics and
pandemics.
56.
Methods For Determining Antimicrobial Drug Resistance
The present invention provides for the prediction or detection of antimicrobial drug resistance in a microorganism, particularly in a pathogenic microorganism, by determining the presence or absence of antimicrobial resistance markers (AMRs) in the microorganism. This allows for the rapid determination of whether a microorganism is resistant to one or more antimicrobial drugs. The microorganism can be obtained from a patient suffering or suspected of suffering from an infection by the microorganism, and once the determination is made, the patient can be given an antimicrobial drug to which the microorganism is not resistant.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
G16B 20/00 - ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
A method for quantifying endotoxins in a biological sample with an analysis medium added to the field of view of an imager, the analysis medium including at least one analysis chamber and a plurality of reference chambers, including the steps of, for each of a plurality of measuring times of a measuring period: a) acquiring an image and determining light intensity valves in the reference and analysis chambers; b) determining a calibration relationship connecting the light intensity value and the concentration at this measuring time; c) determination of at least one endotoxin concentration in a biological sample from said calibration relationship and the intensity value in the analysis chamber at said measurement time; the method subsequently including the step of d) determining a temporal evolution of an endotoxin concentration in the biological sample over the course of the measuring period from the endotoxin concentration for a plurality of measurement times.
G01N 21/77 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
58.
METHOD AND KIT FOR DETECTING A REPLICATING RESPIRATORY VIRUS
A method for determining, in vitro or ex vivo, the presence in a patient of an infection by a replicating respiratory virus, including a step i) of determining, in a test sample taken from the mouth or nose of said patient, the level of transcripts of at least one marker gene selected from the interferon-stimulated genes, referred to as ISGs; and associated kits.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
The invention relates to a filtration device for use in solid-phase cytometry, said filtration device being configured to filter a biological sample likely to contain microorganisms and to allow its analysis, the filtration device comprising: a filtration membrane configured to filter the biological sample, the filtration membrane having an upper face and a lower face, characterized in that the filtration device further comprises at least: a coverslip arranged above the upper face of the filtration membrane, the coverslip being arranged so as to cover the entirety of the upper face of the membrane, an interstitial space formed between the coverslip and the upper face of the filtration membrane, the interstitial space being shaped to receive a fluid.
The invention relates to a method for determining the susceptibility of a strain of Mycobacterium tuberculosis to pyrazinamide, the method comprising the following steps: (i) determining the presence in the genome of this strain of at least one mutation in the rpoB gene between positions 761112 and 761182; and (ii) determining the sensitive or resistant nature of this strain depending on the presence or absence of the at least one mutation. The invention also relates to a kit comprising means for detecting at least one sequence chosen from among the sequences SEQ ID NO: 1 to 3 and to the use of this same kit for determining the susceptibility of a strain of Mycobacterium tuberculosis to pyrazinamide.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Chemical preparations for industry and research; chemical preparations for scientific use; chemical reagents and biological preparations for use in science for diagnosis, other than for medical use; reagents and cell culture media other than for medical or veterinary purposes for the control, detection, diagnosis and analysis of contaminating microorganisms and substances in industrial, agri-food, cosmetic and pharmaceutical products; reagents and cell culture media for scientific and research use enabling the control, diagnosis and analysis of contaminating microorganisms and substances for detecting biological threats and monitoring epidemics and pandemics Reagents and cell culture media for medical and veterinary diagnosis; laboratory reagents for use in clinical, medical or veterinary laboratories for medical and veterinary purposes; chemical, biochemical and biological preparations for use in the medical and veterinary fields for the control, detection, diagnosis and analysis of contaminating microorganisms for medical and veterinary diagnostic purposes; reagents and cell culture media for medical and veterinary use enabling the control, diagnosis and analysis of contaminating microorganisms and substances for detecting biological threats and monitoring epidemics and pandemics Diagnostic apparatus and instruments for the detection of microorganisms, not for medical use; laboratory instruments, namely, laboratory instruments for detecting microorganisms; Scientific apparatus and instruments for the monitoring, detection, diagnosis and analysis of contaminating substances in industrial, agri-food, cosmetic, pharmaceutical and veterinary products; scientific apparatus and instruments enabling the control, diagnosis and analysis of contaminating microorganisms and substances for detecting biological threats and monitoring epidemics and pandemics; downloadable software for analyzing, processing and interpreting medical and veterinary test results in the field of in vitro diagnosis Apparatus and instruments for detecting microorganisms for medical and veterinary diagnostic purposes; apparatus and instruments for medical and veterinary use enabling the control, diagnosis and analysis of contaminating microorganisms and substances for detecting biological threats and monitoring epidemics and pandemics
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
(1) Préparations chimiques pour l'industrie et la recherche; préparations chimiques à usage scientifique; réactifs chimiques et préparations biologiques pour le diagnostic, autres qu'à usage médical; réactifs et milieux de culture pour le contrôle, la détection, le diagnostic et l'analyse des microorganismes et des substances contaminantes dans les produits industriels, agro-alimentaires, cosmétiques et pharmaceutiques; réactifs et milieux permettant le contrôle, le diagnostic et l'analyse des microorganismes et des substances contaminantes pour la détection des menaces biologiques et la surveillance des épidémies et pandémies.
(2) Réactifs et milieux de culture pour le diagnostic médical et vétérinaire; réactifs de laboratoire destinés aux laboratoires cliniques, médicaux ou vétérinaires; préparations chimiques, biochimiques et biologiques destinées aux domaines médical et vétérinaire; réactifs et milieux de culture à usage médical et vétérinaire permettant le contrôle, le diagnostic et l'analyse des microorganismes et des substances contaminantes pour la détection des menaces biologiques et la surveillance des épidémies et pandémies.
(3) Appareils et instruments de diagnostic, non à usage médical; appareils et instruments scientifiques d'analyse et de diagnostics, autres qu'à usage médical; instruments de laboratoire; appareils et instruments scientifiques pour le contrôle, la détection, le diagnostic et l'analyse des substances contaminantes dans les produits industriels, agroalimentaires, cosmétiques, pharmaceutiques et vétérinaires; appareils et instruments scientifiques permettant le contrôle, le diagnostic et l'analyse des microorganismes et des substances contaminantes pour la détection des menaces biologiques et la surveillance des épidémies et pandémies; logiciels pour l'analyse, le traitement et l'interprétation de résultats de tests médicaux et vétérinaires dans le domaine du diagnostic in vitro.
(4) Appareils et instruments pour le diagnostic médical et vétérinaire; appareils et instruments à usage médical et vétérinaire permettant le contrôle, le diagnostic et l'analyse des microorganismes et des substances contaminantes pour la détection des menaces biologiques et la surveillance des épidémies et pandémies.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Chemicals used in industry, Chemical preparations for research; Chemical preparations for scientific purposes; Chemical diagnostic reagents, Biological diagnostic preparations, not for medical purposes; Reagents and Growing media, for use in the following fields: Monitoring, detection, diagnosis and analysis of microorganisms and contaminants in industrial goods, agri-foodstuffs, cosmetics and pharmaceuticals; Reagents and media for monitoring, diagnosing and analysing microorganisms and contaminants, for use in the following fields: Detection of biological threats and surveillance of epidemics and pandemics. Reagents and culture media, for use in the following fields: medical diagnosis and Veterinary diagnosis; Laboratory reagents for clinical, medical or veterinary laboratories; Chemical, biochemical and biological preparations, for use in the following fields: Medicine and Veterinary medicine; Reagents and media enabling the monitoring, diagnosis and analysis of microorganisms and contaminants, For medical purposes and Veterinary use, for use in the following fields: Detection of biological threats and surveillance of epidemics and pandemics. Diagnostic apparatus and instruments, not for medical purposes; Scientific analysis and diagnostic apparatus and instruments, Not for medical purposes; Laboratory instruments; Scientific apparatus and instruments, for use in the following fields: Monitoring, detection, diagnosis and analysis of contaminants in industrial goods, agri-foodstuffs, cosmetics, pharmaceuticals and veterinary goods; Scientific apparatus and instruments for monitoring, diagnosing and analysing microorganisms and contaminants, for use in the following fields: Detection of biological threats and surveillance of epidemics and pandemics; Software for analysis, processing and interpretation of medical and veterinary test results in the field of in vitro diagnosis. Instruments and apparatus for medical and veterinary diagnosis; Apparatus and instruments for monitoring, diagnosing and analysing microorganisms and contaminants, For medical and veterinary purposes, for use in the following fields: Detection of biological threats and surveillance of epidemics and pandemics.
64.
GENETIC RESISTANCE PREDICTION AGAINST ANTIMICROBIAL DRUGS IN MIRCOORGANISMS USING STRUCTUREAL CHANGES IN THE GENOME
The invention relates to a method of determining an infection of a patient with at least one microorganism, particularly a bacterial microorganism, potentially resistant to antimicrobial drug treatment, a method of selecting a treatment of a patient suffering from an infection with at least one microorganism, particularly bacterial microorganism, and a method of determining structural changes of the genome of the microorganism, particularly bacterial microorganism, comprising at least one gene, as well as Computer program products used in these methods.
G16B 15/00 - ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
G16B 20/00 - ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
G16B 20/20 - Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
G16B 30/00 - ICT specially adapted for sequence analysis involving nucleotides or amino acids
G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
65.
USING THE FULL REPERTOIRE OF GENETIC INFORMATION FROM BACTERIAL GENOMES AND PLASMIDS FOR IMPROVED GENETIC RESISTANCE TESTS
The invention relates to a method of determining an antimicrobial drug resistance profile for a microorganism, wherein nucleic acid sequences of the microorganism are analyzed for at least two genetic variations of the nucleic acid sequences comprising at least one genetic variation in a chromosome and at least one genetic variation in at least one plasmid, as well as a, e.g. diagnostic, method of determining an infection of a patient with a microorganism potentially resistant to antimicrobial drug treatment and a method of selecting a treatment of a patient suffering from an infection with a potentially resistant microorganism, wherein the data of the antimicrobial drug resistance profile are applied.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
G16B 20/00 - ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
66.
METHOD USING A TRANSCRIPTOMIC SCORE FOR ASSESSING THE CLINICAL OUTCOME OF A PATIENT IN A CLINICAL SETTING, AND MEANS FOR ITS IMPLEMENTATION
The invention relates to a method of determining, from an assayed sample or a population of assayed samples previously drawn from patients, a transcriptomic score (TScore) suitable for assessing a risk of adverse clinical outcome for patients in a clinical setting, said method involving, amongst other steps, the measurement of gene expression values on a patient sample or a population of samples previously obtained from patients for at least two distinct genes selected among a predetermined set of genes, and the determination of a transcriptomic score where the genes are assigned or not assigned a point. The method can be computer-implemented. The genes of the predetermined set of genes can be at least two genes selected among: ADGRE3, ARL14EP, BPGM, C3AR1, CCNB1IP1, CD177, CD274, CD3D, CD74, CIITA, CTLA4, CX3CR1, GNLY, IFNgamma, IL10, IL1R2, IL1RN, IL7R, IP10/CXCL10, MDC1, OAS2, S100A9, TAP2, TDRD9, TNF and ZAP70. The method can be implemented on assayed biological sample(s) drawn from patient(s) which have been admitted in a reanimation unit, an intensive or continuing care unit. The invention also relates to a method to classify a sample previously drawn from a patient, in a group reflecting a risk of adverse clinical outcome in a clinical setting, or to identify a patient at risk of adverse clinical outcome in a clinical setting, as well as an in vitro or ex vivo method of screening whether a drug has the capacity to alleviate an adverse clinical outcome in a patient. The invention also relates to computer means for implementing the invention, and the use of a kit for carrying out a method of the invention.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
67.
SYSTEMS AND METHODS FOR TARGETING TESTING PANEL BASED ON TEMPORAL, GEOGRAPHIC, AND DEMOGRAPHIC DATA
Methods for targeting the results provided by a testing panel based on patient population data are provided, comprising: identifying, by one or more processors, a set of relevant pathogen groups for each of a respective one or more populations of interest; obtaining, by the one or more processors, pathogen testing results for a particular individual, corresponding to a plurality of pathogen groups; and reporting, by the one or more processors, to a device associated with an individual or a medical provider of the individual, the pathogen testing results for the particular individual, corresponding to the set of relevant pathogen groups for a population, of the one or more populations of interest, with which the individual is associated.
G01N 33/487 - Physical analysis of biological material of liquid biological material
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
G16H 50/80 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for detecting, monitoring or modelling epidemics or pandemics, e.g. flu
G16H 10/00 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data
68.
MODIFIED IMMUNO-MOLECULAR ASSAY FOR FASTER BIOMARKER DETECTION
The present invention relates to an improved immuno-molecular assay, for example a Proximity Extension Assay protocol or a Proximity Ligation Assay (PLA) protocol, which is more compatible with the assay durations that are expected in the context of point-of-care diagnostics. In particular, the time needed for performing the Proximity Extension Assay protocol of the invention is reduced by more than 13-folds as compared to classical Proximity Extension Assay protocols, without significantly affecting the performance of the assay for protein detection. Thus, the method of the invention provides a more rapid and simpler procedure to screen and diagnose diseases in a non-invasive way. The use of such a method in an integrated system is an exciting and expected improvement in clinical diagnostics for the detection of biomarkers in human samples.
G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
C12Q 1/6804 - Nucleic acid analysis using immunogens
69.
METHOD FOR DETERMINING THE RISK OF INCIDENCE OF A CARE-RELATED INFECTION IN A PATIENT
An in vitro or ex vivo method for determining the risk of incidence of a healthcare-associated infection in a patient, including a step of measuring the expression of at least one gene encoding a pro-inflammatory cytokine receptor located on chromosome 2 in the region 2q11-2q12, in a biological sample from the patient.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific apparatus, instruments and devices, not for medical use, namely, applicators for plates for collection and detection of contaminants in industrial, agri-food, cosmetic and pharmaceutical products and in the production environment for these products, namely, water, air, and surfaces environments, and for in-vitro diagnosis
71.
METHOD FOR DETECTING THE PRESENCE OF POLYMERS IN A SPECTRUM OBTAINED BY MASS SPECTROMETRY
The invention relates to a method for detecting the presence of a signal from a polymer in spectra obtained by mass spectrometry of biological samples, the method comprising a step (101) of determining a set of at least one parameter, referred to as a descriptor, of the presence of the polymer, a step (103) of assigning a score to each value of each descriptor, and a step (105) of classifying the scores into a class of positive spectra containing no polymer and a class of negative spectra containing the polymer.
G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
G16C 20/00 - Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
It is disclosed a method for performing fluorescence measurement on a sample, comprising: Illuminating the sample using a light source, acquiring at least one fluorescence image of the illuminated sample, and processing the fluorescence image to determine a fluorescence intensity of the sample, characterized in that processing the fluorescence image to determine a fluorescence intensity of the sample comprises: extracting, from the fluorescence image, a region of interest (ROI) free from artefacts, by application of a trained model, and determining the fluorescence intensity of the sample from the extracted region of interest.
The disclosure relates to a sample collection device (1, 100) comprising a support (10, 110) arranged to collect and release microbiological fluids, the sample collection device further comprising a head (7, 107), said head (7, 107) being configured to cooperate with a spout (6) of an analysis device (2) in such a way that the cooperation between the head (7, 107) and the spout (6) causes microbiological fluid to be released inside the analysis device (2).
A61B 10/00 - Instruments for taking body samples for diagnostic purposesOther methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determinationThroat striking implements
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Chemical preparations for industry and research; chemical
reagents and biological preparations for diagnosis, other
than for medical use; chemical preparations for scientific
use; reagents and culture media for controlling, detecting,
diagnosing and analyzing contaminating substances in
industrial, agri-food, cosmetic, pharmaceutical and
environmental products. Reagents and culture media for medical, pharmaceutical and
veterinary diagnosis; laboratory reagents for clinical or
medical laboratories; biological preparations for medical or
veterinary use.
C07D 417/06 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism
80.
CULTURE MEDIUM FOR DETECTING MICROORGANISMS COMPRISING A MIXTURE OF AGAR AND KAPPA-CARRAGEENAN AS GELLING AGENT
The invention relates to the field of molecular biology and more particularly that of clinical and industrial microbiology. More specifically, the present invention relates to a culture medium for detecting microorganisms, comprising, as a gelling agent, a mixture of agar and kappa-carrageenan.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
The present invention relates to a method of determining an infection of a patient with at least one microorganism, particularly a bacterial microorganism, potentially resistant to antimicrobial drug treatment, a method of selecting a treatment of a patient suffering from an infection with at least one microorganism, particularly bacterial microorganism, and a method of determining an antimicrobial drug, e.g. antibiotic, resistance profile for at least one microorganism, particularly bacterial microorganism, as well as computer program products used in these methods.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
A method for quantifying at least one microorganism group via at least one mass spectrometry analysis is described. The method includes at least one separation and fragmentation step. The method moreover includes a step that involves measuring the amount of at least one representative peptide or at least one protein representing the microorganism group. The at least one representative peptide or the at least one protein is obtained after the at least one separation and fragmentation step and serves as a quantification marker(s). The amount of the quantification marker(s) is directly correlatable to the amount of the at least one microorganism group.
A system for diluting a sample of biological material, including a fluid circuit, wherein the fluid circuit includes at least: a first container configured to contain a sample of biological material containing a biological material to be diluted, the sample being a fluid; a second container configured to contain a first dilution fluid; and at least one first metering member for metering a predetermined volume of fluid, including a first wall and a second wall, the first metering member including a metering zone configured to change at least from an initial state in which the first wall and the second wall are in contact with one another to an operating state in which the first wall and the second wall are separated from one another.
C07K 16/24 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
An analysis plate configured to allow a characterization of microorganisms by mass spectrometry, wherein the analysis plate includes at least one analysis zone configured for a biological sample containing a population of at least one microorganism, wherein all or part of the analysis zone is made of a porous material, the analysis zone including at least one antimicrobial agent.
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
G16B 25/10 - Gene or protein expression profilingExpression-ratio estimation or normalisation
Detection method for sample presence and device associated The invention relates to a detection method of liquid presence inside a well of a transparent or translucent support intended for analysis performed in a device for in vitro detection and/or quantification, the presence of liquid being assessed on the basis of an index of visible pattern likelihood representative of the probability of a pattern to be legible as expected in the field of view of an imager. Said invention allows preventing assay execution without sample and can be applied without wasting sample or sample well support in case of the sample well is not filled by the user. Moreover, this method allows a precise detection of the samples that are transparent or translucent, with low reflectance.
The present invention relates to a method for in vitro or ex vivo detection of an immunocompromised status in a subject, comprising quantifying, in a biological sample of said subject, the expression of at least 2 target genes selected from the group consisting of TAP2, C3AR1, CD177 and IL1R2, and identifying whether or not a variation in their expression is present as compared to a reference value. The invention also relates to tools for implementing said method, and to uses thereof.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
The present disclosure relates to methods and systems suitable for simultaneously amplifying a number of potential targets and then performing a melt detection based on whether or not any amplification is detected for any target nucleic acid(s), wherein the melt parameters are limited by the melt temperature range characteristic for the target nucleic acid(s) for which amplification is detected. This invention retains the resolution achieved by nucleic acid melts with a slow, steady ramp rate between the annealing and denaturation temperatures, but does so faster by using faster ramp rates at temperatures where no melt signature is expected from the reaction, and slower at temperatures where a melt signature is expected from the reaction.
A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
The present invention relates to in vitro or ex vivo methods and kits for determining the viral or bacterial nature of an infection on the basis of a biological sample of a patient by identifying the change in the level of expression of a plurality of biomarkers. In particular, the method comprises the steps of (a) measuring the expression of at least one viral target gene and of at least one bacterial target gene chosen from among OLAH, FAM20A, IL1R2, MMP8, RETN and SLC1A2; (b) comparing the expressions measured in step (a) with reference expression values for these target genes; and (c) on the basis of the results of the comparison, concluding whether the infection is viral or bacterial in nature .
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
93.
SYSTEMS, METHODS, AND APPARATUSES FOR CONCENTRATION AND IDENTIFICATION OF A MICROORGANISM FROM BLOOD
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
94.
METHODS AND COMPOSITIONS FOR TREATMENT OF RENAL INJURY AND RENAL FAILURE
A method of devising a therapy plan for renal replacement therapy (RRT) includes detecting a level of one or more biomarkers in a body fluid sample obtained from a subject. The level(s) may be correlated to an expected benefit from treatment with continued RRT and/or to an expected ability to successfully discontinue RRT. The method may include the step of assigning the subject to a predetermined subpopulation of individuals exhibiting a known status with regard to meeting criteria for continuing or discontinuing RRT. In some embodiments, the biomarker level is detected by introducing the body fluid into an assay instrument and contacting the body fluid to a binding reagent, for example, an antibody.
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
G16H 20/40 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance relating to mechanical, radiation or invasive therapies, e.g. surgery, laser therapy, dialysis or acupuncture
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
G01N 33/48 - Biological material, e.g. blood, urineHaemocytometers
G01N 33/487 - Physical analysis of biological material of liquid biological material
A method of analyzing a biological sample including biological agents and disposed in an analysis receptacle in a field of view of a holographic imaging system defining an acquisition focal plane, including, for each of a plurality of measurement times: acquiring a plurality of holographic images of the biological sample at different respective positions of the acquisition focal plane, and, from each acquired holographic image, determining a value of a biomass parameter representative of the quantity of biological agents at the position of the acquisition focal plane, the method including constructing a distribution indicator from values of the biomass parameter at the same measurement time for a plurality of positions of the acquisition focal plane, and providing, among the analysis results, a representation of the distribution of the biomass of biological agents, derived from at least one distribution indicator at a measurement time.
G01N 21/45 - RefractivityPhase-affecting properties, e.g. optical path length using interferometric methodsRefractivityPhase-affecting properties, e.g. optical path length using Schlieren methods
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
98.
METHOD FOR CLASSIFYING AN INPUT IMAGE REPRESENTING A PARTICLE IN A SAMPLE
A method for classifying at least one input image representing a target particle in a sample, involves implementing, by data processing of a client, steps of: (b) extracting the characteristic map of the target particle by a convolutional neural network pre-trained on a base of public images; (c) classifying the input image according to the extracted characteristic map.
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
G06V 10/24 - Aligning, centring, orientation detection or correction of the image
G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersectionsConnectivity analysis, e.g. of connected components
G06V 10/774 - Generating sets of training patternsBootstrap methods, e.g. bagging or boosting
99.
METHOD AND SYSTEM FOR CHARACTERISING MICROORGANISMS BY DIGITAL HOLOGRAPHIC MICROSCOPY
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) (France)
Inventor
Brault, Dylan
Fournier, Corinne
Olivier, Thomas
Faure, Nicolas
Abstract
The invention relates to a method for characterising microorganisms present in a biological sample, the method comprising acquiring a live holographic digital image of the sample, generating a focused image by using a digital model for reconstructing the focused image and characterising the bacteria on the basis of the focused image. According to the invention, prior to the holographic image being acquired, a plurality of calibration objects are provided in the acquisition field of view, which objects have a previously characterised dimension and refractive index and a shape chosen so that the interference patterns of the objects can be calculated using an image forming model for incorporating optical aberrations of the acquisition device. Prior to the focused image being computer generated, the optical aberrations are qualified according to the interference patterns of the objects and the calculated interference patterns and the digital model for reconstructing the focused image incorporates the quantified optical aberrations.
G03H 1/00 - Holographic processes or apparatus using light, infrared, or ultraviolet waves for obtaining holograms or for obtaining an image from themDetails peculiar thereto
G03H 1/04 - Processes or apparatus for producing holograms
The subject matter is a method for determining the proliferation capacity of T lymphocytes in a subject, the method including the following steps: a) measuring the load of torque teno virus from a biological sample of the subject; and b) determining the proliferative capacity of the T lymphocytes based on the viral load measured in a).
C12Q 1/6881 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
