A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
Assembly comprising a sample collection vessel and a separation container having seal, plunger with seal-piercing point, retainer, and flexible sealing member
A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
System, heaters, and heat transfer devices are disclosed. For example, a system for performing polymerase chain reaction includes a support member configured to receive a sample vessel and a heater that is positioned to affect a temperature of the sample vessel. The system additionally includes a heat transfer device disposed between the heater and the sample vessel. The heat transfer device illustratively includes anisotropic fibers axially aligned parallel to one another and positioned to conduct heat from the at least one heater toward the sample vessel in the axial direction of the anisotropic fibers. An exemplary heater includes a body defining one or more channels, a heating element positioned in the one or more channels, and retention members adjacent the one or more channels. At least a portion of the heating element is mechanically interlocked with the channel by deforming the retention members into a closed position.
Optical systems and apparatuses configured for enabling substantially simultaneous observation of a plurality of points in an array from a common reference point. Without the optical systems and apparatuses disclosed herein, less than all of the plurality of points can be observed substantially simultaneously from the common reference point.
A solid fluorescence standard that can be used to calibrate and/or normalize a device (e.g., a scientific instrument) that is configured for generating and collecting fluorescence data. A fluorescence standard disclosed herein includes an adhesive (e.g., a low viscosity, substantially optically transparent, solvent-free, radiation curable adhesive, such as, but not limited to, a UV curable adhesive), and a selected quantity of fluorescent particles (e.g., quantum dots) dispersed in the adhesive. The adhesive and the fluorescent particles are mixed together and disposed in a sample well. The adhesive is then cured and solidified, which yields a solid fluorescence standard in the well.
G01N 21/27 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
G16B 30/00 - ICT specially adapted for sequence analysis involving nucleotides or amino acids
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
B04B 5/04 - Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
G01N 1/20 - Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
B04B 11/04 - Periodical feeding or dischargingControl arrangements therefor
A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
B04B 5/04 - Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
G01N 1/20 - Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
B04B 11/04 - Periodical feeding or dischargingControl arrangements therefor
24.
Centrifugally separating samples in a container having a seal and containing a plunger for opening the seal
A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
G01N 1/20 - Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
B04B 11/04 - Periodical feeding or dischargingControl arrangements therefor
25.
Isolation tube with a rheological control member and a plunger
A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
B04B 5/04 - Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
C12Q 1/18 - Testing for antimicrobial activity of a material
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
G01N 1/20 - Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
B04B 11/04 - Periodical feeding or dischargingControl arrangements therefor
A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.
A solid fluorescence standard that can be used to calibrate and/or normalize a device (e.g., a scientific instrument) that is configured for generating and collecting fluorescence data. A fluorescence standard disclosed herein includes an adhesive (e.g., a low viscosity, substantially optically transparent, solvent-free, radiation curable adhesive, such as, but not limited to, a UV curable adhesive), and a selected quantity of fluorescent particles (e.g., quantum dots) dispersed in the adhesive. The adhesive and the fluorescent particles are mixed together and disposed in a sample well. The adhesive is then cured and solidified, which yields a solid fluorescence standard in the well.
G01N 21/27 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
28.
SYSTEMS AND METHODS FOR POINT OF USE EVACUATION OF AN ARRAY
Optical systems and apparatuses configured for enabling substantially simultaneous observation of a plurality of points in an array from a common reference point. Without the optical systems and apparatuses disclosed herein, less than all of the plurality of points can be observed substantially simultaneously from the common reference point.
System, heaters, and heat transfer devices are disclosed. For example, a system for performing polymerase chain reaction includes a support member configured to receive a sample vessel and a heater that is positioned to affect a temperature of the sample vessel. The system additionally includes a heat transfer device disposed between the heater and the sample vessel. The heat transfer device illustratively includes anisotropic fibers axially aligned parallel to one another and positioned to conduct heat from the at least one heater toward the sample vessel in the axial direction of the anisotropic fibers. An exemplary heater includes a body defining one or more channels, a heating element positioned in the one or more channels, and retention members adjacent the one or more channels. At least a portion of the heating element is mechanically interlocked with the channel by deforming the retention members into a closed position.
G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups
H05B 3/14 - Heating elements characterised by the composition or nature of the materials or by the arrangement of the conductor characterised by the composition or nature of the conductive material the material being non-metallic
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
G06F 19/22 - for sequence comparison involving nucleotides or amino acids, e.g. homology search, motif or Single-Nucleotide Polymorphism [SNP] discovery or sequence alignment
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
A solid fluorescence standard that can be used to calibrate and/or normalize a device (e.g., a scientific instrument) that is configured for generating and collecting fluorescence data. A fluorescence standard disclosed herein includes an adhesive (e.g., a low viscosity, substantially optically transparent, solvent-free, radiation curable adhesive, such as, but not limited to, a UV curable adhesive), and a selected quantity of fluorescent particles (e.g., quantum dots) dispersed in the adhesive. The adhesive and the fluorescent particles are mixed together and disposed in a sample well. The adhesive is then cured and solidified, which yields a solid fluorescence standard in the well.
G01N 21/27 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
B01F 13/00 - Other mixers; Mixing plant, including combinations of dissimilar mixers
B01J 13/00 - Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided forMaking microcapsules or microballoons
B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
A temperature cycling system (10, 110) is provided for repeatedly heating and cooling a reaction mixture (16). The system (10, 110) includes a first heater (27) and a second heater (28) each movable between a first orientation in which the first or second heater (27, 28) affects the temperature of the reaction mixture (16) and a second orientation in which the first or second heater (27, 28) does not substantially affect the temperature of the reaction mixture (16). During temperature cycling, the second heater (28) is in the second orientation when the first heater (27) is in the first orientation, and the second heater (28) is in the first orientation when the first heater (27) is in the second orientation.
Tools and methods are provided for removing a plurality of tubes from a rack of tubes. Tube rack tools include a base and/or top piece for receiving the rack of tubes. The base has a support surface for engaging the closed bottom of the plurality of tubes. The support surface can be curved such that the rack of tubes can be rocked about the curves support surface to dislodge the plurality of tubes from the rack. The top piece can be configured to engage the rack such that pressure on the top piece in the direction of the support surface applies a pressure on the rack without applying the pressure to the tubes. The tubes are dislodged from the rack by application of the pressure. Elements of the tube rack tool are also configured for securing the tubes within the rack or caps to the tubes.
Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5′ of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells. The apparatus can also be manufactured to provide uniform configurations. For example, a sample illustratively for polymerase chain reaction (PCR), in each sample well and the components of the embodiment of the thermal cycler apparatus shown at including a well block, a base plate, a layer of adhesive, a peltier device, another layer of adhesive and a heat sink.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Fluorescent dyes, chemical dyes, chemical dye mixtures, reagents, diagnostic preparations, chemical buffers that minimize change in pH, and kits comprised primarily of the foregoing, for medical laboratory, veterinary laboratory, industrial laboratory use and scientific laboratory use and scientific and research use, all for scanning, detecting, testing, analyzing, expressing, identifying, tagging, labelling, classifying, measuring, counting, determining sequence, amplifying and mapping cells, genes, nucleic acids and biological samples. Fluorescent dyes, chemical dyes, chemical dye mixtures, reagents, diagnostic preparations, chemical buffers that minimize change in pH, and kits comprised primarily of the foregoing, for clinical and medical diagnostic, all for scanning, detecting, testing, analyzing, expressing, identifying, tagging, labelling, classifying, measuring, counting, determining sequence, amplifying and mapping cells, genes, nucleic acids and biological samples.
Tools and methods are provided for removing a plurality of tubes from a rack of tubes. Tube rack tools include a base and/or top piece for receiving the rack of tubes. The base has a support surface for engaging the closed bottom of the plurality of tubes. The support surface can be curved such that the rack of tubes can be rocked about the curves support surface to dislodge the plurality of tubes from the rack. The top piece can be configured to engage the rack such that pressure on the top piece in the direction of the support surface applies a pressure on the rack without applying the pressure to the tubes. The tubes are dislodged from the rack by application of the pressure. Elements of the tube rack tool are also configured for securing the tubes within the rack or caps to the tubes.
Optical systems and apparatuses configured for enabling substantially simultaneous observation of a plurality of points in an array from a common reference point. Without the optical systems and apparatuses disclosed herein, less than all of the plurality of points can be observed substantially simultaneously from the common reference point.
An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells. The apparatus can also be manufactured to provide uniform configurations. For example, a sample, illustratively for polymerase chain reaction (PCR), in each sample well and the components of the embodiment of the thermal cycler apparatus shown at including a well block, a base plate, a layer of adhesive, a peltier device, another layer of adhesive and a heat sink.
A temperature cycling system (10, 110) is provided for repeatedly heating and cooling a reaction mixture (16). The system (10, 110) includes a first heater (27) and a second heater (28) each movable between a first orientation in which the first or second heater (27, 28) affects the temperature of the reaction mixture (16) and a second orientation in which the first or second heater (27, 28) does not substantially affect the temperature of the reaction mixture (16). During temperature cycling, the second heater (28) is in the second orientation when the first heater (27) is in the first orientation, and the second heater (28) is in the first orientation when the first heater (27) is in the second orientation.
Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
C09B 23/04 - Methine or polymethine dyes, e.g. cyanine dyes characterised by the methine chain containing an odd number of CH groups one CH group, e.g. cyanines, isocyanines, pseudocyanines
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C07D 413/06 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
C07D 417/06 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
C09B 23/04 - Methine or polymethine dyes, e.g. cyanine dyes characterised by the methine chain containing an odd number of CH groups one CH group, e.g. cyanines, isocyanines, pseudocyanines
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C07D 413/06 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
C07D 417/06 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
Methods are provided for nucleic acid analysis. In an illustrative method, allele amplification bias is used to amplify preferentially a target nucleic acid that is present in a low allele fraction.
An experimental melting curve is modeled as a sum of a true melting curve and background fluorescence. A deviation function may be generated based upon the experimental melting curve data and a model of a background signal. The deviation function may be generated by segmenting a range of the experimental curve into a plurality of windows. Within each window, a fit between the model of the background signal and the experimental melting curve data may be calculated. The deviation function may be formed from the resulting fit parameters. The deviation function may include background signal compensation and, as such, may be used in various melting curve analysis operations, such as data visualization, clustering, genotyping, scanning, negative sample removal, and the like. The deviation function may be used to seed an automated background correction process. A background-corrected melting curve may be further processed to remove an aggregation signal.
G06F 3/01 - Input arrangements or combined input and output arrangements for interaction between user and computer
G06F 3/042 - Digitisers, e.g. for touch screens or touch pads, characterised by the transducing means by opto-electronic means
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
G06F 19/24 - for machine learning, data mining or biostatistics, e.g. pattern finding, knowledge discovery, rule extraction, correlation, clustering or classification
G06F 19/20 - for hybridisation or gene expression, e.g. microarrays, sequencing by hybridisation, normalisation, profiling, noise correction models, expression ratio estimation, probe design or probe optimisation
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Fluorescent dyes, chemical dyes, chemical dye mixtures, reagents, diagnostic preparations, chemical buffers that minimize change in pH, and kits comprised primarily of the foregoing, for medical and medical laboratory diagnostic use, and for clinical and medical laboratory use, all for scanning, detecting, testing, analyzing, expressing, identifying, tagging, labeling, classifying, measuring, counting, determining sequence, amplifying, and mapping cells, genes, nucleic acids and biological samples
Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5′ of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Reagents for scientific and research purposes, reagents for industrial use, namely, testing food, water, blood, air, and other liquids, powders and substances, chemicals, namely, chemical buffers and buffer solutions used in testing food, water, blood, air, and other liquids, powders and substances, enzymes for scientific and research purposes, enzymes for industrial use, namely, testing food, water, blood, air, and other liquids, powders and substances, DNA genetic primers, DNA nucleic acid primers, biochemical reagents commonly known as probes, genetic probes and nucleic acid probes for detecting and analyzing molecules in food, water, blood, air, and other liquids, powders and substances, diagnostic preparations for scientific and research purposes, diagnostic preparations for industrial use, namely, testing food, water, blood, air, and other liquids, powders and substances, diagnostic preparations for environmental diagnostic, environmental clinical and environmental laboratory use, and kits and assay kits comprised primarily of assays for research and industrial use, reagents for research and industrial use, chemical buffers, buffer solutions, genetic primers, nucleic acid primers, probes, genetic probes, nucleic acid probes, enzymes for research and industrial use and diagnostic preparations for research and industrial use, with all of the above for use in detecting, identifying, classifying, tagging, amplifying, testing, analyzing, evaluating, monitoring, purifying, counting and measuring chemical, biochemical, biological, genetic, pathological and toxic agents, pathogens, bacteria, germs, viruses, diseases, contagions, materials and samples; Reagents for environmental diagnostic, environmental clinical use and environmental laboratory use; Enzymes for environmental diagnostic use, environmental clinical use, environmental laboratory use and industrial use Equipment, instruments, devices and apparatus, namely, digital optical equipment comprising fluorimeters used to record fluorescence signals and produce digital data, thermal control elements for heating and cooling, light emitters consisting of optical diodes, lenses, and stations for placement of sample containers, all for detecting, identifying, classifying, tagging, amplifying, testing, analyzing, evaluating, monitoring, purifying, counting, measuring, preparing, mixing, heating and cooling chemical, biochemical, biological, genetic, pathological and toxic agents, pathogens, bacteria, germs, viruses, diseases, contagions, materials and samples for scientific, research and laboratory purposes; materials, articles and disposable items, namely, transformer connectors, batteries, battery chargers, pouches for samples, pouch holders, carrying packs for carrying all of the above-named goods, plastic or other sampling vials, plastic or other preparation vials, syringes and plungers, for scientific, research and laboratory use, and for use with scientific, research and laboratory equipment, instruments, devices and apparatus, transformer connectors, batteries and battery chargers for medical, veterinary, clinical and diagnostic use and for use with medical, veterinary, clinical and diagnostic equipment, instruments, devices and apparatus; and computer software, computer programs and associated instruction manuals sold as a unit all for use in detecting, identifying, classifying, tagging, amplifying, testing, analyzing, evaluating, monitoring, purifying, counting, measuring, preparing, testing, mixing, heating and cooling chemical, biochemical, biological, genetic, pathological and toxic agents, pathogens, bacteria, germs, viruses, diseases, contagions, materials and samples, and for storing and analyzing experimental and other data, for scientific, research, laboratory, medical, veterinary, clinical, diagnostic, industrial, biological, pathological, genetic, environmental, dietary and agricultural purposes; and Probes for environmental purpose
Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analysis include nucleic acid amplification and detection and immuno-PCR.
Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
42 - Scientific, technological and industrial services, research and design
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Reagents, buffers, chemical buffers, fluorescent dyes, chemical dyes, chemical dye mixtures, and kits comprised of the foregoing, for scientific, research, industrial and quality control purposes; and control solutions and control reagents, and kits comprised of the foregoing, for use with scientific and research apparatus for quality control purposes, for use with scientific and research instruments for quality control purposes, and for use with scientific and research devices for quality control purposes; diagnostic reagents, buffers, chemical buffers, fluorescent dyes, chemical dyes, chemical dye mixtures, and kits comprised of the foregoing, for laboratory use in the environmental fields for treating environments or for use in testing the condition of environments; and control solutions and control reagents, and kits comprised of the foregoing, for treating environments or for use in testing the condition of environments, all for use with laboratory apparatus for monitoring the accuracy and precision of tests in the environmental fields, for use with laboratory instruments for monitoring the accuracy and precision of tests in the environmental fields, and for use with laboratory devices for monitoring the accuracy and precision of tests in the environmental fields [Diagnostic reagents, buffers, chemical buffers, fluorescent dyes, chemical dyes, chemical dye mixtures, and kits comprised of the foregoing, for laboratory use in the medical, biological, pathological, pharmaceutical, and genetic fields; and control solutions and control reagents, and kits comprised of the foregoing, for use with laboratory apparatus for monitoring the accuracy and precision of tests in the medical, biological, pathological, pharmaceutical, and genetic fields, for use with laboratory instruments for monitoring the accuracy and precision of tests in the medical, biological, pathological, pharmaceutical, and genetic fields, and for use with laboratory devices for monitoring the accuracy and precision of tests in the medical, biological, pathological, pharmaceutical, and genetic fields] [Scientific and laboratory research and development services, design services for others, scientific and laboratory research and development testing services, diagnostic services, scientific and laboratory research and development analysis services, scientific and laboratory sample scanning services for scientific purposes, product development services for tools, devices and instruments, software development and maintenance services, scientific, laboratory, research and development consulting services, and scientific, laboratory, research and development online information services, all in the fields of clinical diagnostic assays, clinical diagnostic tests, disease screening, genetic disease screening, disease identification, genetic disease identification, disease testing, genetic disease testing, mutation detection, mutation screening, mutation scanning, genomics, functional genomics, genome testing, genome analysis, genotyping, gene testing, gene identification, gene analysis, gene screening, gene interaction screening, gene expression, gene mapping, genetic marker identification, genetic trait determination, plant genetics, genetic modifications, genetically modified organisms, genetically modified products, DNA fingerprinting, nucleic acid testing, nucleic acid analysis, identity testing, human identity testing, person identification, donor identification, tissue typing, tissue matching, parentage testing, paternity testing, forensics, pharmaceuticals, pharmaceutical testing, pharmaceutical identification, agriculture, agricultural products, agricultural uses, quality testing of samples, purity testing of samples, and quality control of samples] [Devices, equipment and instruments, namely, fluorimeters, imaging devices that utilize fluorescent light to create digital photographs, thermal control elements specially adapted for scientific use for heating and cooling samples, mutation scanners, genotype scanners, microtiter plate readers, and apparatus, all for detecting, identifying, classifying, analyzing, evaluating, monitoring, preparing, testing, mixing, heating and cooling nucleic acid, cells, genes and chemical, biochemical, biological, genetic and pathological materials and samples for scientific, research and laboratory purposes; materials, articles, apparatus and disposable items, namely, optical filters, computer chips, heating elements specially adapted for scientific use for heating samples for testing purposes, sample containers specially adapted for scientific use, namely, containers used to hold samples for testing purposes, tubes made of various materials specially adapted for scientific use for testing purposes, and microtiter plates, all for scientific, research and laboratory use, and for use with scientific, research and laboratory devices, equipment, instruments, apparatus, imaging devices and microtiter plate readers; computer software, computer programs and computer firmware all for use in controlling processes, inputting, analyzing, modifying, transferring, displaying, reporting and storing data and results, for scientific, research, laboratory, medical, clinical, diagnostic, industrial, biological, pathological, pharmaceutical, genetic, environmental and agricultural purposes, and for use in gene, nucleic acid and other testing and analysis, in particular for clinical diagnostic assays, clinical diagnostic tests, disease screening, genetic disease screening, disease identification, genetic disease identification, disease testing, genetic disease testing, mutation detection, mutation screening, mutation scanning, genomics, functional genomics, genome testing, genome analysis, genotyping, gene testing, gene identification, gene analysis, gene screening, gene interaction screening, gene expression, gene mapping, genetic marker identification, genetic trait determination, plant genetics, genetic modification testing, genetically modified organism testing, genetically modified products testing, DNA fingerprinting, nucleic acid testing, nucleic acid analysis, amino acid testing, identity testing, human identity testing, person identification, donor identification, tissue typing, tissue matching, parentage testing, paternity testing, forensics, pharmaceuticals, pharmaceutical testing, pharmaceutical identification, agriculture, agricultural products, agricultural uses, quality testing, purity testing, and quality control; computer software, computer programs and computer firmware all for use in controlling processes, detecting, analyzing, measuring, testing, identifying, classifying, tagging, labeling, determining sequence, counting, mapping, engineering, expressing, amplifying, heating, cooling, measuring the temperature of, and monitoring cells, genes, nucleic acids and biological samples for laboratory, research, development, genetic, biological, pathological, medical, clinical, diagnostic, industrial, pharmaceutical, agricultural, environmental, chemical, biochemical, biomedical, bioinformatics, biotechnological and scientific purposes; and computers, laptop computers, hard drives for computers, and computer hardware] [Devices, equipment and instruments, namely, fluorimeters, imaging devices that utilize fluorescent light to create digital photographs, thermal control elements specially adapted for medical use for heating and cooling samples, mutation scanners, genotype scanners, microtiter plate readers, and apparatus, all for detecting, identifying, classifying, analyzing, evaluating, monitoring, preparing, testing, mixing, heating and cooling nucleic acid, cells, genes and chemical, biochemical, biological, genetic, pathological and toxic materials and samples for medical, clinical and diagnostic purposes; and materials, articles, apparatus and disposable items, namely, heating elements specially adapted for medical use for heating samples for testing purposes, containers specially adapted for medical use to hold samples for testing purposes, tubes made of various materials specially adapted for medical use for testing purposes, and microtiter plates, all for medical, clinical and diagnostic use, and for use with medical, clinical and diagnostic devices, equipment, instruments, apparatus, imaging devices and microtiter plate readers]
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
chemical products, namely, reagents, buffers and diagnostic preparations for scientific, research and industrial purposes; control solutions and control reagents for use with scientific and research apparatus, instruments and devices for quality control and calibration purposes; and kits comprised of reagents, buffers and diagnostic preparations for use with scientific and research apparatus, instruments and devices for quality control and calibration purposes [ reagents, buffers and in vitro diagnostic agents for medical, veterinary, biological, pathological, pharmaceutical, genetic and environmental clinical, diagnostic and laboratory use; control solutions and control reagents for use with medical, veterinary, biological, pathological, pharmaceutical, genetic and environmental clinical, diagnostic and laboratory apparatus, instruments and devices for monitoring the accuracy and precision of medical tests; and kits comprised of control solutions and control reagents for monitoring the accuracy and precision of medical tests ] [ Laboratory apparatus, instruments and devices for analyzing nucleic acid, cells, genes and biological samples, namely, digital imaging devices comprising fluorimeters used to record fluorescence signals and produce digital data, thermal control elements for heating and cooling, light emitters such as lasers and light bulbs, lenses, mirrors, stations for placement of microtiter plates, microtiter plate readers, computers, instrument software, analytical software, and cases to hold the foregoing, for scientific, research and laboratory purposes; laboratory apparatus, materials, articles and disposable items, namely, pipettes, plastic and metal receiver cartridges, and microtiter plates, for scientific, research and laboratory use, and for use with scientific, research and laboratory apparatus, instruments and devices; computer software and computer programs for use in storing and analyzing experimental and other data for scientific, research, laboratory, medical, veterinary, clinical diagnostic, industrial, biological, pathological, pharmaceutical, genetic, environmental, dietary and agricultural purposes; and laptop computers, hard drives for computers, and computer hardware ] [ medical apparatus, instruments and devices, namely, digital imaging devices comprising fluorimeters used to record fluorescence signals and produce digital data, thermal control elements for heating and cooling, light emitters such as lasers and light bulbs, lenses, mirrors, stations for placement of microtiter plates, microtiter plate readers, computers, instrument software, analytical software, and cases to hold the foregoing, for analyzing nucleic acid, cells, genes and biological samples for medical, surgical, veterinary, clinical and diagnostic purposes; and medical apparatus, materials, articles and disposable items, namely, plastic and metal receiver cartridges, and microtiter plates, for medical, surgical, veterinary, clinical and diagnostic use, and for use with medical, surgical, veterinary, clinical and diagnostic apparatus, instruments and devices ]
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
fluorescent dyes, chemical dyes, chemical dye mixtures, reagents, diagnostic preparations, chemical buffers that minimize change in pH, and kits comprised primarily of the foregoing, for scientific or research use, all for scanning, detecting, testing, analyzing, expressing, identifying, tagging, labeling, classifying, measuring, counting, determining sequence, amplifying, and mapping cells, genes, nucleic acids and biological samples [ fluorescent dyes, chemical dyes, chemical dye mixtures, reagents, diagnostic preparations, chemical buffers that minimize change in pH, and kits comprised primarily of the foregoing, for clinical, medical diagnostic, medical laboratory, veterinary laboratory, and industrial laboratory use, all for scanning, detecting, testing, analyzing, expressing, identifying, tagging, labeling, classifying, measuring, counting, determining sequence, amplifying, and mapping cells, genes, nucleic acids and biological samples ]
