Provided herein are methods of producing cell lines with stable expression of a transgene by removal of CpG motifs. In further methods, there are provided methods for cell lines with stable expression of a transgene by driving expression by novel promoters or by tagging endogenous genes.
The present disclosure provides extracellular vesicle-enriched compositions derived from iPSC-derived microglia. Further provided herein are methods for using the extracellular vesicle-enriched compositions from iPSC-derived microglia in screening assays to discover properties, including therapeutic properties.
The present disclosure provides extracellular vesicle-enriched compositions derived from iPSC-derived microglia. Further provided herein are methods for using the extracellular vesicle-enriched compositions from iPSC-derived microglia in screening assays to discover properties, including therapeutic properties.
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
4.
METHODS AND COMPOSITIONS FOR IPSC-DERIVED MICROGLIA
The present disclosure provides iPSC-derived microglia comprising a protective CD33 allele. Further provided herein are assays for screening analytes and genes associated with the protective CD33 allele.
Provided herein are methods for producing quiescent cardiac fibroblast cells from pluripotent stem cells. Further provided herein are microtissue compositions comprising the cardiac fibroblast cells with endothelial cells and cardiomyocytes.
Provided herein are methods for producing quiescent cardiac fibroblast cells from pluripotent stem cells. Further provided herein are microtissue compositions comprising the cardiac fibroblast cells with endothelial cells and cardiomyocytes.
The present disclosure provides iPSC-derived dopaminergic neurons comprised disease-associated mutations. Further provided herein are methods for using the iPSC-derived dopaminergic neurons for the study of neuroinflammation, such as to identify novel targets, biomarkers, and therapeutic agents for the diagnosis, prognosis, and treatment of neurodegenerative diseases. Further provided herein are assays for studying neuroinflammation using the present cell culture models.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
8.
IPSC-DERIVED ASTROCYTES AND METHODS OF USE THEREOF
The present disclosure provides method for producing neural precursor cells with a glial bias from induced pluripotent cells and further differentiating the neural precursor cells to astrocytes. Further provided herein are methods for the use of the astrocytes for screening assays, models mimicking the human brain, and cell therapy.
The present disclosure provides method for producing neural precursor cells with a glial bias from induced pluripotent cells and further differentiating the neural precursor cells to astrocytes. Further provided herein are methods for the use of the astrocytes for screening assays, models mimicking the human brain, and cell therapy.
The present disclosure relates generally to methods and systems for the analysis, development, testing, and/or optimization, of culture media and/or culture conditions for extracellular vesicle production; culture media analyzed, developed, tested, and/or optimized via such assays; and to extracellular vesicles and compositions comprising extracellular vesicles generated using such culture media.
Examples herein include methods, systems, and computer program products for utilizing neural networks in ultrasound systems. The methods include processor(s) of a computing device obtaining an image that depicts cells. The processor(s) applies one or more nuclei detection algorithms to detect nuclear aspects in the image. The processor(s) generates a nuclear segmentation map. The processor(s) utilizes the nuclear segmentation map to identify one or more regions of interest in the image. The processor(s) generates a classification result by automatically determining a cell type for each cell in a region of interest of the regions of interest.
The present disclosure provides multicellular culture models for the study of neuroinflammation, such as to identify novel targets, biomarkers, and therapeutic agents for the diagnosis, prognosis, and treatment of neurodegenerative diseases. Further provided herein are assays for studying neuroinflammation using the present cell culture models.
Provided herein are methods for the differentiation of pluripotent stem cells to committed cardiac progenitor cells. Further provided herein are methods for the use of the committed cardiac progenitor cells in the treatment of cardiac disorders.
Provided herein are methods of producing cell lines with stable expression of a transgene by removal of CpG motifs. In further methods, there are provided methods for cell lines with stable expression of a transgene by driving expression by novel promoters or by tagging endogenous genes.
The present disclosure provides methods of producing hepatocytes from induced pluripotent stem cells. Further provided herein are methods of using the hepatocytes for the treatment of a liver disease.
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