Abstract

Provided is a method for producing differentiated cells, which makes it possible to separate differentiated cells and cells that are not differentiated satisfactorily from each other with a simple and inexpensive configuration without using a chemical substance. A method for producing differentiated cells according to the present invention is characterized by performing each of the following steps at least once: a differentiation induction step for inducing the differentiation of cells: and a size separation step for subjecting a particle suspension solution containing the cells obtained by the differentiation induction step to the separation into particles each having a size equal to or larger than a predetermined value and particles each having a size smaller than the predetermined value on the basis of a hydrodynamic effect.

IPC Classes  ?

• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• C12M 1/26 - Inoculator or sampler

Abstract

A forge bonding machine includes: a supporting body supporting a lower-surface side of bonding portion of members to be bonded in a state where the members are layered; a pressurizing body applying pressure on an upper-surface side of the bonding portion in the state where the members are layered; a stroke controller controlling a gap between the supporting and the pressurizing bodies; and a heater raising a temperature of the bonding portion to a predetermined temperature range by directly or indirectly coming into contact with the members, in which the stroke controller controls a reduction ratio R (T0/T1) that represents a ratio of a thickness T0 of the bonding portion before bonding to a thickness T1 after the bonding, and the supporting body or/and the pressurizing body comprise a rod controlled in terms of stroke toward the bonding portions by a displacement meter or a stopper.

IPC Classes  ?

• B23K 20/02 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating by means of a press
• B21J 5/06 - Methods for forging, hammering, or pressingSpecial equipment or accessories therefor for performing particular operations

Abstract

[Problem] To provide a nanoparticle production method whereby desired nanoparticles can be easily produced using a microfluidic device. [Solution] A method for manufacturing nanoparticles according to the present invention comprises a step for introducing a first liquid including at least one material through a first fluid inlet flow path 10a of a microfluidic device 1a into a mixing flow path 20, and introducing a second liquid into the mixing flow path 20 through a second fluid inlet flow path 10b and a third fluid inlet flow path 10c on the sides of the first fluid inlet flow path 10a. The Reynolds number in the mixing flow path 20 is set to a predetermined value or more, and the flow of the first liquid in the mixing flow path 20 is made asymmetric so that the flow of the first liquid immediately after entering the mixing flow path 20 through the first fluid inlet flow path 10a is biased toward one of both side walls of the mixing flow path 20.

IPC Classes  ?

• B82B 3/00 - Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
• B01F 33/3011 - Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
• B01J 13/02 - Making microcapsules or microballoons
• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle

Abstract

The method comprises applying a spot load to a joint part between a first metal material and a second metal material in a state where sites to form the joint part are superposed on each other. When a total thickness of the first metal material and the second metal material at the joint part before bonding is defined as T0 mm, the total thickness thereof after bonding is defined as T1 mm, and T0/T1=R is defined as a reduction ratio, the reduction ratio R is 1.4 or more.

IPC Classes  ?

• B23K 20/02 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating by means of a press
• B23K 20/227 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating taking account of the properties of the materials to be welded with ferrous layer
• B23K 103/20 - Ferrous alloys and aluminium or alloys thereof

Abstract

The present invention provides a mucosal adjuvant comprising a compound represented by formula (I): A-L-B (In the formula, A represents a structure having TLR7 activity, L represents a linker, and B represents a structure having TLR2 activity.), or a pharmaceutically acceptable salt thereof.

IPC Classes  ?

• A61K 39/39 - Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
• A61K 9/08 - Solutions
• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• A61K 39/002 - Protozoa antigens
• A61K 39/02 - Bacterial antigens
• A61K 39/12 - Viral antigens
• A61K 39/35 - Allergens
• A61K 39/145 - Orthomyxoviridae, e.g. influenza virus
• A61K 39/215 - Coronaviridae, e.g. avian infectious bronchitis virus
• A61P 31/00 - Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• A61P 31/04 - Antibacterial agents
• A61P 31/10 - Antimycotics
• A61P 31/12 - Antivirals
• A61P 31/14 - Antivirals for RNA viruses
• A61P 31/16 - Antivirals for RNA viruses for influenza or rhinoviruses
• A61P 33/00 - Antiparasitic agents
• A61P 37/04 - Immunostimulants
• C07D 473/16 - Heterocyclic compounds containing purine ring systems with oxygen, sulfur, or nitrogen atoms directly attached in positions 2 and 6 two nitrogen atoms

Abstract

01011 mm is the total thickness of the first metal material and the second metal material after joining.

IPC Classes  ?

• H01M 50/564 - Terminals characterised by their manufacturing process
• B23K 20/00 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating
• H01M 50/562 - Terminals characterised by the material
• H01R 4/62 - Connections between conductors of different materialsConnections between or with aluminium or steel-core aluminium conductors
• H01R 13/03 - Contact members characterised by the material, e.g. plating or coating materials
• H01R 43/16 - Apparatus or processes specially adapted for manufacturing, assembling, maintaining, or repairing of line connectors or current collectors or for joining electric conductors for manufacturing contact members, e.g. by punching and by bending

Abstract

01011 of the welded portions after welding.

IPC Classes  ?

• B23K 20/00 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating

Abstract

Provided is a hollow spherical particle that can be prepared with superior safety and greater ease. A hollow spherical particle according to the present invention is a self-organizing body of lignin, wherein the content of the lignin in a β-O-4 ether structure is 50% or greater, and the solubility with respect to 60 to 80% ethanol at 25°C is 2 w/v% or greater.

IPC Classes  ?

• C08J 3/14 - Powdering or granulating by precipitation from solutions
• C09K 11/06 - Luminescent, e.g. electroluminescent, chemiluminescent, materials containing organic luminescent materials
• A61Q 17/04 - Topical preparations for affording protection against sunlight or other radiationTopical sun tanning preparations
• B01J 13/12 - Making microcapsules or microballoons by phase separation removing solvent from the wall-forming material solution
• A61K 8/73 - Polysaccharides
• C09K 3/00 - Materials not provided for elsewhere
• C09K 23/50 - Derivatives of lignin

Abstract

[Problem] The purpose of the present invention is to provide: a sealing structure that has excellent bonding strength and reliability and can be bonded by means of a simple process in a short amount of time; and a production method for the same. [Solution] A sealing structure for sealing and holding an internal structure or a component that is provided inside, said sealing structure characterized by comprising a case body and a sealing member that is sealed and bonded to the case body by a sealing-bonding part, wherein the sealing-bonding part is a diffusion bonding part for similar or dissimilar metals, and the diffusion bonding part has a plastic flow layer formed thereon.

IPC Classes  ?

• F16J 15/04 - Sealings between relatively-stationary surfaces without packing between the surfaces, e.g. with ground surfaces, with cutting edge

Abstract

1=R is defined as a reduction ratio, the reduction ratio R is 1.4 or more.

IPC Classes  ?

• B23K 20/00 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating
• B23K 20/02 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating by means of a press
• B23K 20/227 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating taking account of the properties of the materials to be welded with ferrous layer
• B23K 103/20 - Ferrous alloys and aluminium or alloys thereof

Abstract

Provided is a method for evaluating virus-neutralizing antibodies using dried blood adhering to filter paper, the method including: a step for mixing a blood sample obtained by extracting the dried blood adhering to the filter paper and a pseudotype virus, which coats the coat protein of a target virus and in the genes of which a reporter gene is incorporated, to obtain a mixture; a step for infecting a susceptible cultured cell line with the psuedotype virus in the mixture; and a step for detecting the presence or measuring the amount of pseudotype-virus-neutralizing antibodies in the blood sample on the basis of the expression level of the reporter gene after infection.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• C12N 7/01 - Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• C12Q 1/66 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving luciferase
• C12Q 1/6897 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

The present invention addresses the problem of providing a novel and effective antibody that binds to the spike protein of SARS-CoV-2, and in particular addresses the problem of providing an antibody that inhibits binding between SARS-CoV-2 and ACE2 or an antibody that inhibits the entry of SARS-CoV-2 into the cell. The present invention provides, for example, an antibody that has the following combination of complementarity-determining regions (CDRs) and that binds to the spike protein of SARS-CoV-2, or an antigen-binding fragment from said antibody: CDRH1, 2, and 3 and CDRL1, 2, and 3 composed of the amino acid sequences of SEQ ID NOS: 9, 10, 11, 20, 21, and 22.

IPC Classes  ?

• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
• A61P 31/14 - Antivirals for RNA viruses
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 15/13 - Immunoglobulins
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12P 21/08 - Monoclonal antibodies
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C07K 14/165 - Coronaviridae, e.g. avian infectious bronchitis virus

Abstract

A method for producing a metal pattern by processing a substrate having on its surface a metal layer with a photosensitive fiber having a specific composition, a method for producing a metal pattern, and a composition for producing the photosensitive fiber. The photosensitive fiber contains a positive photosensitive material. The positive photosensitive material may contain a novolac resin, etc. The method for producing a metal pattern includes a first step of forming a fiber layer of photosensitive resin on a substrate having on its surface a metal layer; a second step of exposing the fiber layer to light via a mask; a third step of developing the fiber layer with a developer to thereby form a photosensitive fiber pattern; and a fourth step of etching the metal layer with an etchant and removing the photosensitive fiber, to thereby form a network metal pattern.

IPC Classes  ?

• G03F 7/039 - Macromolecular compounds which are photodegradable, e.g. positive electron resists
• G03F 7/09 - Photosensitive materials characterised by structural details, e.g. supports, auxiliary layers
• D01F 6/36 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from copolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds comprising unsaturated carboxylic acids or unsaturated organic esters as the major constituent
• D01F 6/34 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from copolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds comprising unsaturated alcohols, acetals, or ketals as the major constituent
• G03F 7/20 - ExposureApparatus therefor
• H05K 3/02 - Apparatus or processes for manufacturing printed circuits in which the conductive material is applied to the surface of the insulating support and is thereafter removed from such areas of the surface which are not intended for current conducting or shielding

Abstract

[Problem] To provide a joining method for a metal material which is excellent in intensity by suppressing generation of an IMC of a joint portion, and is high in productivity. [Solution] A joining method for a metal material characterized in that, in a state in which parts as a joint portion between a first metal material and a second metal material are overlapped, a spot load is applied on the joint portion by a pressurization means.

IPC Classes  ?

• B23K 20/00 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating

Abstract

[Problem] The present invention addresses the problem of providing: a method for producing a metal pattern by processing a substrate having a metal layer at a surface thereof, using photosensitive fibers having a specific composition; a production method for a metal pattern; and a composition for producing the photosensitive fibers, and preferably providing a transparent interconnect pattern that is low-cost and is flexible. [Solution] The present invention provides a photosensitive fiber containing a positive photosensitive material. The positive photosensitive material may contain a novolac resin or the like. The production method for a metal pattern according to the present invention includes a first step for forming a fiber layer containing photosensitive fibers on a substrate having a metal layer at a surface thereof, a second step for exposing the fiber layer with light through a mask, a third step for developing the fiber layer using a developer liquid to form a photosensitive fiber pattern, and a fourth step for etching the metal layer using an etchant, and removing the photosensitive fibers, to form a reticulated metal pattern.

IPC Classes  ?

• D01F 1/10 - Other agents for modifying properties
• G03F 7/023 - Macromolecular quinonediazidesMacromolecular additives, e.g. binders
• G03F 7/20 - ExposureApparatus therefor
• G03F 7/40 - Treatment after imagewise removal, e.g. baking

Abstract

According to the present invention, the ratio of direct charging, which is the expenditure amount by medical procedure for each organization in a business analysis of a medical facility, is improved. Provided is a system for assisting calculation of medical facility accounts, the system comprising: an order management unit that generates a consumable article management order having patient attributes, which include a patient identifier, and order attributes, which include an order identifier and organization information, the order being generated on the basis of a medical procedure order that has the patient attributes and the order attributes; and an expenditure processing unit that associates an identifier of an article consumed in the medical procedure with a consumable article management order pertaining to the medical procedure, reads the identifier, refers to an article master in which the identifier of the article and the article cost are matched, adds an article attribute including the article cost to the consumable article management order with which the article is associated, and generates a direct charging consumable article list.

IPC Classes  ?

• G06Q 40/00 - FinanceInsuranceTax strategiesProcessing of corporate or income taxes
• G16H 40/20 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the management or administration of healthcare resources or facilities, e.g. managing hospital staff or surgery rooms

Abstract

[Problem] The purpose of the present invention is to provide a distillation device effective for imparting excellent liquor quality and that can be manufactured easily at low cost, for the production of distilled liquor. [Solution] A distillation device characterized by comprising a pot part, a head part provided to the upper part of the pot part, and a lyne arm part provided to the tip of the head part, wherein at least one among the pot part, the head part, and the lyne arm part is made from a copper alloy casting.

IPC Classes  ?

• C12H 6/02 - Methods for increasing the alcohol content of fermented solutions or alcoholic beverages by distillation

Abstract

[Problem] To provide a particle separation device which is easy to operate and whereby particles can be appropriately separated. [Solution] This device has: a syringe 2; a barrel 41 into which a sample liquid S including target particles is injected; a barrel 51 into which a buffer liquid B is injected; a branching tube 3 provided with a first conduit 31 connected to a discharge port 21a of the syringe 2, a second conduit 32 as one fork branched from the first conduit 31, connected to an injection port of the barrel 41, and a third conduit 33 as the other fork branched from the first conduit 31, connected to an injection port of the barrel 51; a first unidirectional valve 31a for backflow prevention, for opening when a syringe side thereof has a positive pressure and closing when the syringe side thereof has negative pressure, the first unidirectional valve 31a intervening partway along the first conduit opening; and a DLD microchannel chip 6 provided with a DLD microchannel structure.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/28 - Inoculator or sampler being part of container

Abstract

The invention provides a method capable of conveniently producing an intricate and fine resist pattern. The invention also provides a fiber containing a positive-type or negative-type photosensitive material.

IPC Classes  ?

• D01D 5/00 - Formation of filaments, threads, or the like
• G03F 7/039 - Macromolecular compounds which are photodegradable, e.g. positive electron resists
• G03F 7/038 - Macromolecular compounds which are rendered insoluble or differentially wettable
• G03F 7/022 - Quinonediazides
• D01F 6/50 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from mixtures of polymers obtained by reactions only involving carbon-to-carbon unsaturated bonds as major constituent with other polymers or low-molecular-weight compounds of polyalcohols, polyacetals or polyketals
• D01F 1/10 - Other agents for modifying properties

Abstract

The purpose of the present invention is to provide a method by which a complicated fine resist pattern is able to be easily produced. The present invention relates to fibers which contain a positive or negative photosensitive material.

IPC Classes  ?

• D01F 1/10 - Other agents for modifying properties
• D01D 5/04 - Dry spinning methods
• D01F 6/50 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from mixtures of polymers obtained by reactions only involving carbon-to-carbon unsaturated bonds as major constituent with other polymers or low-molecular-weight compounds of polyalcohols, polyacetals or polyketals
• G03F 7/022 - Quinonediazides
• G03F 7/038 - Macromolecular compounds which are rendered insoluble or differentially wettable
• G03F 7/039 - Macromolecular compounds which are photodegradable, e.g. positive electron resists

Abstract

The invention provides a fiber containing (A) a polymer compound containing a structural unit having, in a side chain, at least one kind of organic group selected from a hydroxy group, a hydroxymethyl group and an alkoxymethyl group having 1-5 carbon atoms, and (B) a photoacid generator.

IPC Classes  ?

• D01F 6/14 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from homopolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds from polymers of unsaturated alcohols, e.g. polyvinyl alcohol, or of their acetals or ketals
• G03F 7/09 - Photosensitive materials characterised by structural details, e.g. supports, auxiliary layers
• D01D 5/00 - Formation of filaments, threads, or the like
• D01F 1/10 - Other agents for modifying properties
• D01F 2/28 - Monocomponent artificial filaments or the like of cellulose or cellulose derivativesManufacture thereof from cellulose derivatives from organic cellulose esters or ethers, e.g. cellulose acetate
• D01F 6/26 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from homopolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds from other polymers
• C08F 220/56 - AcrylamideMethacrylamide
• C08L 3/02 - StarchDegradation products thereof, e.g. dextrin
• G03F 7/038 - Macromolecular compounds which are rendered insoluble or differentially wettable

Abstract

The present invention provides a composition for forming a biocompatible coating film on a substrate (e.g., resinous substrate). The present invention relates to a composition for forming biocompatible coating films which comprises (A) an organic polymer and (D) a C2-5 methoxy alcohol as a solvent. The organic polymer (A) preferably includes a structural unit having, in a side chain, at least one organic group selected from among a hydroxy group, a hydroxymethyl group, and C1-5 alkoxymethyl groups.

IPC Classes  ?

• C09D 201/00 - Coating compositions based on unspecified macromolecular compounds
• C08J 7/04 - Coating
• C09D 7/12 - Other additives
• C09D 133/14 - Homopolymers or copolymers of esters of esters containing halogen, nitrogen, sulfur or oxygen atoms in addition to the carboxy oxygen
• C09D 133/26 - Homopolymers or copolymers of acrylamide or methacrylamide
• G03F 7/004 - Photosensitive materials
• G03F 7/038 - Macromolecular compounds which are rendered insoluble or differentially wettable
• G03F 7/09 - Photosensitive materials characterised by structural details, e.g. supports, auxiliary layers

Abstract

The present invention provides a photosensitive composition which includes a copolymer containing constitutional units represented by formulae (1)-(3), a photoacid generator, and a solvent (the definition of the groups in the formulae is as described in the specification).

IPC Classes  ?

• G03F 7/038 - Macromolecular compounds which are rendered insoluble or differentially wettable
• C08F 220/56 - AcrylamideMethacrylamide
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus

Abstract

[Problem] To provide a low-cost imprint template with which the trapping of air can be reduced in photocurable imprinting, and which has excellent transferability to a substrate having curvature, and to provide a method for manufacturing this imprint template. [Solution] A template used in UV nano-imprinting, said template characterized by having a flexible, light-transmitting fixed plate (1c), a transparent resin buffer layer (1b) formed on top of the fixed plate, and a resin film mold (1a) adhered in a removable manner to the top of the transparent resin buffer layer (1b), with one or a combination of a plurality of the resin film molds (1a) forming an irregular transfer pattern on the surface thereof.

IPC Classes  ?

• H01L 21/027 - Making masks on semiconductor bodies for further photolithographic processing, not provided for in group or
• B29C 33/38 - Moulds or coresDetails thereof or accessories therefor characterised by the material or the manufacturing process
• B29C 59/02 - Surface shaping, e.g. embossingApparatus therefor by mechanical means, e.g. pressing

Abstract

Provided is a UV light generator that is capable of generating uniformly a UV light having high emission intensity. A UV lamp (10) is formed from an electrically insulating discharge tube (5) formed into a loop shape, a plurality of segmented electrodes (6) disposed along the discharge tube (5) so as to adopt the same loop shape as the discharge tube (5), and magnets (2, 3, 4) which are disposed surrounding the discharge tube (5) and form a closed magnetic field inside the discharge tube (5). The segmented electrodes (6) of the UV lamp (10) are connected to a multiphasic alternating current power source wherefrom a multiphasic alternating discharge voltage is applied to segmented electrodes (6) that are next to each other out of phase from each other, and through the discharge plasma (P) generated in so doing, a UV light is generated.

IPC Classes  ?

• H01J 65/00 - Lamps without any electrode inside the vesselLamps with at least one main electrode outside the vessel
• F21S 2/00 - Systems of lighting devices, not provided for in main groups or , e.g. of modular construction
• H01J 1/50 - Magnetic means for controlling the discharge
• H01J 61/56 - One or more circuit elements structurally associated with the lamp

Abstract

[Problem] The present invention addresses the problem of providing an inflammatory cytokine activity inhibitor which is non-invasive and satisfies requirements such as safety, convenience and economic performance, for the purpose of treating diabetes and preventing the development of diabetic complications and as a therapy method for altering the natural history of diabetes, or as an effective means for preventing the progression of diabetes. The present invention also addresses the problem of providing a therapeutic or prophylactic agent for autoinflammatory diseases for which an activity of inhibiting the activity of inflammatory cytokines is effective. [Solution] The inflammatory cytokine activity inhibitor is characterized by containing, as an active ingredient, at least one compound selected from an alkaloid originated from a plant belonging to the family Menispermaceae, the genus Stephania, a derivative of the alkaloid and a pharmaceutically acceptable salt of the alkaloid or the derivative.

IPC Classes  ?

• A61K 36/18 - Magnoliophyta (angiosperms)
• A23L 1/30 - containing additives (A23L 1/308 takes precedence);;
• A61K 31/4741 - QuinolinesIsoquinolines ortho- or peri-condensed with heterocyclic ring systems condensed with ring systems having oxygen as a ring hetero atom, e.g. tubocuraran derivatives, noscapine, bicuculline
• A61P 3/00 - Drugs for disorders of the metabolism
• A61P 3/10 - Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
• A61P 29/00 - Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agentsNon-steroidal antiinflammatory drugs [NSAID]
• A61P 43/00 - Drugs for specific purposes, not provided for in groups

Abstract

[Problem] To provide a safe deodorant that maintains a high deodorizing effect over an extended period of time and is nontoxic to plants and animals, and a method for producing the same. [Solution] Rice bran is fermented by effective microorganisms, and a deodorant is made from a liquid collected from the fermented rice bran. The effective microorganisms are at least one among bacteria of the genus Pediococcus, yeast cells, microorganisms of the genus Bacillus, streptococci, and staphylococci. The above-mentioned liquid contains a component having deodorizing activity against the odor of amines. This component is at least one among lactic acid, acetic acid, propionic acid, butyric acid, and succinic acid. The above-mentioned liquid also contains a component having deodorizing activity against the odor of acids. This component is at least one among spermidine, spermine, and putrescine. The above-mentioned liquid also contains a component having deodorizing activity against the odor of aldehydes.

IPC Classes  ?

• A61L 9/01 - Deodorant compositions
• C12P 13/00 - Preparation of nitrogen-containing organic compounds
• C12P 21/00 - Preparation of peptides or proteins

Abstract

[Problem] The purpose of the present invention is to produce (-)-vibo-quercitol with high efficiency by a simple process. Particularly, it is intended to utilize an enzyme capable of converting 2-deoxy-scyllo-inosose to (-)-vibo-quercitol directly. [Solution] A 2-deoxy-scyllo-inosose reductase which is originated from a microorganism capable of utilizing (-)-vibo-quercitol and has the properties (a) to (c): (a) the enzyme has a catalytic activity of converting 2-deoxy-scyllo-inosose to (-)-vibo-quercitol; (b) the activity of the enzyme becomes maximum at a pH value of 7.0 to 9.0; and (c) a polypeptide moiety in the enzyme has a molecular mass of about 36 kDa as measured by SDS-polyacrylamide electrophoresis.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12P 7/02 - Preparation of oxygen-containing organic compounds containing a hydroxy group

Abstract

　The objective of the present invention is to provide a fiber allowing shape processing using a direct and simple lithographic method, and enabling forming of complex fine fiber patterns. A fiber including: (A) a polymer compound containing a structural unit having on a side chain at least one organic group selected from a hydroxy, hydroxymethyl, and a C1-5 alkoxymethyl; and (B) a photoacid generator.

IPC Classes  ?

• D01F 6/52 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from mixtures of polymers obtained by reactions only involving carbon-to-carbon unsaturated bonds as major constituent with other polymers or low-molecular-weight compounds of polymers of unsaturated carboxylic acids or unsaturated esters
• C08F 20/54 - Amides
• C08L 5/00 - Compositions of polysaccharides or of their derivatives not provided for in group or
• C08L 33/24 - Homopolymers or copolymers of amides or imides
• D01D 5/04 - Dry spinning methods
• D01F 1/10 - Other agents for modifying properties

Abstract

[Problem] To provide a technique for improving the conception rate and birth rate in mammals having genetic characteristics such as low conception rate and birth rate in pregnancy by natural mating even though ovulation function and the eggs themselves are seemingly normal; or in ova having lowered conception rate and birth rate due to environment, age, and other such factors. [Solution] To remove the fertilized eggs of a mammal exhibiting low conception rate and low birth rate from the body, culture the eggs in vitro using a medium containing a glycine receptor agonist of a specific concentration, then return the embryos to the mother and bring to term.

IPC Classes  ?

• C12N 5/073 - Embryonic cells or tissuesFoetal cells or tissues
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 5/075 - OocytesOogonia
• A01K 67/027 - New or modified breeds of vertebrates

Abstract

The present invention relates to a class of novel pradimicins and analogues and derivatives thereof, including the compounds of formula A, I and 111, and/or a pharmaceutical acceptable addition salt thereof and/or a stereoisomer thereof and/or a solvate thereof and their use to treat or prevent kinetoplastid infections and their use to manufacture a medicine to treat or prevent kinetoplastid infections, particularly infections with trypanosoma and leishmania, such as Trypanosoma brucei, Trypanosoma cruzi and Leischmania donovani. wherein Ra, R1, R2, R3, R4, R5, R6, R7, R8 and R9 are as defined in the claim 1 or as described in detail in the description of the invention. The present invention also relates to pharmaceutical compositions of said compounds and the use of said pharmaceutical compositions to treat or prevent kinetoplastid infections. The present invention further relates to the use of said compounds as biologically active ingredients, more specifically as medicaments for the treatment of kinetoplastid infections and pathologic conditions such as, but not limited to Trypanosomiasis, such as African trypanosomiasis, sleeping sickness, Chagas disease and leishmaniasis.

IPC Classes  ?

• A61K 31/704 - Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin, digitoxin
• A61P 33/02 - Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis

Abstract

There is provided a method for quantifying a subject substance, of which typical examples are amino acids. The method of the present invention comprises the following steps: the step of allowing an enzyme that can generate pyrophosphate by using adenosine triphosphate (ATP) as a substrate with converting the subject substance to act on the subject substance to generate pyrophosphate; the step of allowing pyruvate pyrophosphate dikinase (PPDK) to act on the generated pyrophosphate in the presence of adenosine monophosphate (AMP) and phosphoenolpyruvate (PEP) to generate ATP, phosphoric acid, and pyruvate; and the step of quantifying the generated pyruvate, and amount of the subject substance is determined on the basis of the obtained amount of pyruvate. According to the present invention, an amino acid in a biological sample containing a lot of various kinds of contaminants such as inorganic phosphoric acid and urea can be conveniently and quickly quantified without being influenced by the contaminants.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
• C12Q 1/25 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving enzymes not classifiable in groups
• C12Q 1/32 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving dehydrogenase
• C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase

Abstract

The present invention provides a high-quality raw material for silicic-acid materials which has high solubility in raw materials, e.g., fertilizers, and a process for producing a high-quality raw material for silicic-acid materials. The temperature of an in-furnace atmosphere is heightened to cause the rice hulls to undergo self-burning (exothermic reaction), thereby burning off the readily combustible organic matter contained in the rice hulls and heightening the content of inorganic matter in the rice hulls. Simultaneously therewith, the exothermic reaction of the rice hulls is continued to substantially burn off the difficultly combustible organic matter remaining on the surface of the rice hulls, thereby heightening the content of amorphous silica (SiO2) and improving the solubility.

IPC Classes  ?

• B09B 3/00 - Destroying solid waste or transforming solid waste into something useful or harmless
• C05D 9/00 - Other inorganic fertilisers

Abstract

A method for manufacturing a piezoelectric ceramic, comprising a step for preparing a raw material to include A, B, Ba, and Zr in the composition ratio represented by the general formula (1 - s)ABO3-sBaZrO3 (where A is at least one species of element selected form alkali metals, B is at least one species of transition metal element including Nb, and 0.06 < s ≤ 0.15), a step for molding the raw material to obtain a molded article, a step for firing the molded article in a reducing atmosphere, and a step for heat treating, in an oxidizing atmosphere, the fired body obtained by the firing step.

IPC Classes  ?

• C04B 35/00 - Shaped ceramic products characterised by their compositionCeramic compositionsProcessing powders of inorganic compounds preparatory to the manufacturing of ceramic products
• H01L 41/187 - Ceramic compositions
• H01L 41/22 - Processes or apparatus specially adapted for the assembly, manufacture or treatment of piezo-electric or electrostrictive devices or of parts thereof

Abstract

The purpose of the present invention is to provide a novel formulation technology whereby high drug availability can be achieved without inhibiting the diffusion of a drug contained in a formulation. Provided are: a drug-containing ultrafine fiber characterized by comprising an ultrafine fiber that contains a drug; a drug-containing ultrafine fiber laminate formed by laminating the same; and a preparation to be applied to the skin using the drug-containing ultrafine fiber laminate.

IPC Classes  ?

• A61K 9/00 - Medicinal preparations characterised by special physical form
• A61K 9/70 - Web, sheet or filament bases
• A61K 31/135 - Amines, e.g. amantadine having aromatic rings, e.g. methadone
• A61K 31/167 - Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide having the nitrogen atom of a carboxamide group directly attached to the aromatic ring, e.g. lidocaine, paracetamol
• A61K 31/192 - Carboxylic acids, e.g. valproic acid having aromatic groups, e.g. sulindac, 2-aryl-propionic acids, ethacrynic acid
• A61K 31/196 - Carboxylic acids, e.g. valproic acid having an amino group the amino group being directly attached to a ring, e.g. anthranilic acid, mefenamic acid, diclofenac, chlorambucil
• A61K 31/355 - Tocopherols, e.g. vitamin E
• A61K 31/405 - Indole-alkanecarboxylic acidsDerivatives thereof, e.g. tryptophan, indomethacin
• A61K 31/4402 - Non-condensed pyridinesHydrogenated derivatives thereof only substituted in position 2, e.g. pheniramine, bisacodyl
• A61K 31/56 - Compounds containing cyclopenta[a]hydrophenanthrene ring systemsDerivatives thereof, e.g. steroids
• A61K 47/10 - AlcoholsPhenolsSalts thereof, e.g. glycerolPolyethylene glycols [PEG]PoloxamersPEG/POE alkyl ethers
• A61K 47/14 - Esters of carboxylic acids, e.g. fatty acid monoglycerides, medium-chain triglycerides, parabens or PEG fatty acid esters
• A61K 47/18 - AminesAmidesUreasQuaternary ammonium compoundsAmino acidsOligopeptides having up to five amino acids
• A61K 47/32 - Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers
• A61K 47/34 - Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers
• A61K 47/42 - ProteinsPolypeptidesDegradation products thereofDerivatives thereof, e.g. albumin, gelatin or zein
• A61L 15/44 - Medicaments

Abstract

The present invention provides a chip which has a function of forcibly aligning cells on a microwell by a magnetic action, and in which fluorescence emitted from the cells in the microwell can be observed from the bottom side of the chip. The chip according to the present invention is a microwell array chip provided with a microwell layer on at least one main surface of a light-permeable base plate, wherein each of multiple microwells in the microwell layer has such a shape and size that only one biological cell can be contained per microwell, the bottom surface of each of the microwells is non-toxic to biological cells and is formed from a surface of a light-permeable barrier member, a magnetic member is arranged between the bottom surface formed from the barrier member and the surface of the base plate, and the magnetic member has at least one opening part as observed in a plan view of the bottom surface of each of the microwells. The present invention also relates to a method for placing a cell to be tested in at least some of microwells in the microwell array and observing the cell placed in the microwells, wherein fluorescence emitted from the cell in each of the microwells is observed from the side of the bottom of the base plate through the opening part formed in the magnetic member.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
• G01N 15/00 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials
• G01N 15/10 - Investigating individual particles
• G01N 33/48 - Biological material, e.g. blood, urineHaemocytometers
• G01N 37/00 - Details not covered by any other group of this subclass

Abstract

Provided is a method for quantifying a target substance typified by amino acid. This method includes: a step in which an enzyme capable of converting the target substance and also of generating a pyrophosphate using an adenosine tri-phosphate (ATP) as a base material therefor is caused to act on the target substance, and pyrophosphate is generated; a step in which a pyruvate phosphate dikinase (PPDK) is caused to act on the generated pyrophosphate, in the presence of adenosine monophosphate (AMP) and phosphoenolpyruvic acid (PEP), and ATP, phosphoric acid, and pyruvic acid is generated; and a step in which the generated pyruvic acid is quantified. The amount of target substance is then determined on the basis of the obtained pyruvic acid amount. As a result of this invention, amino acid in a sample derived from an organism including a large quantity of a variety of impurities such as inorganic phosphoric acid and urea can be readily and quickly quantified without being affected by the impurities.

IPC Classes  ?

• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
• C12Q 1/25 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving enzymes not classifiable in groups
• C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
• C12Q 1/32 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving dehydrogenase
• C12N 15/09 - Recombinant DNA-technology

Abstract

Provided are a method for measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
• C12Q 1/28 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving peroxidase

Abstract

An objective of the present invention is to provide a particulate body sterilization device with which it is possible to sterilize a large quantity of particulate bodies uniformly and without unevenness, as well as efficiently. A particulate body sterilization device (1) comprises: an intake (29) wherein a particulate body is received; a duct for sterilization (23), further comprising a sterilization processing space (37) for applying sterilizing electromagnetic waves on a particulate body flow (W1) which is received from the intake (29) and sterilization processing the particulate body flow (W1), and a discharge port (31) for discharging the particulate body which is sterilization processed in the sterilization processing space (37); an airflow generating device (9) which generates an air flow which flows through the sterilization processing space (37) from the intake (29) toward the discharge port (31), for conveying the particulate body flow (W1) from the intake (29) to the discharge port (31); and an airflow adjustment member (39) which adjusts the direction in which the air flow that is generated by the airflow generating device (9) flows in the duct for sterilization (23).

IPC Classes  ?

• A61L 2/10 - Ultraviolet radiation
• A23L 3/28 - Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating with ultraviolet light

Abstract

A dozing-off detection method and device are capable of accurately detecting repeated blinking by using a simple device, and exhibit improved speed and accuracy in dozing-off detection. When the eyes of a person transition from a closed state to an open state, the state during which the eyes are mostly open is considered eyes-open time, while all other states are considered eyes-closed time. A period of time that is relatively shorter than the average interval between blinking for a healthy adult in a state of wakefulness is set as a first threshold interval. A period of time that is relatively longer than the average amount of time that a healthy adult in a state of wakefulness closes their eyes is set as a second threshold interval. When eyes are detected to be open for a shorter time than the first threshold interval, the blinking therebefore and thereafter is considered repeated blinking. Dozing off is determined to have occurred when the time eyes are closed, during the blinking that occurs during said repeated blinking after the eyes are open for a shorter time than the first threshold interval, is equal to or longer than the second threshold interval. Dozing off is immediately determined to have occurred when the time eyes are closed, during the blinking that occurs during said repeated blinking before the eyes open for less than the first threshold interval, is equal to or longer than the second threshold interval. Dozing off is also determined to have occurred when eyes are closed for a relatively longer time than the second threshold interval.

IPC Classes  ?

• A61B 5/16 - Devices for psychotechnicsTesting reaction times
• A61B 5/18 - Devices for psychotechnicsTesting reaction times for vehicle drivers
• G08G 1/16 - Anti-collision systems

Abstract

A chip useful for treating cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. The chip comprises a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing the chip comprises applying a composition containing a crosslinkable temperature-responsive polymer on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.

IPC Classes  ?

• C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism

Abstract

Disclosed is a method for quantifying L-tryptophan involving a step for mixing a sample, L-tryptophan oxidase, and water, a step for allowing the obtained reaction solution to stand a predetermined period of time in the presence of oxygen, and a step for measuring the reaction product resulting from action of enzymes present in the reaction solution after allowing to stand. The L-tryptophan oxidase has a given amino acid sequence and has oxidase activity that generates hydrogen peroxide and ammonia by acting on the L-tryptophan in the presence of oxygen and water. The oxidase activity of the L-tryptophan oxidase on the L-phenylalanine is in the range of 0-3% of the oxidase activity thereof on the L-tryptophan, and the L-tryptophan oxidase does not have oxidase activity on protein-constituting amino acids other than L-tryptophan and L-phenylalanine. Also disclosed are a kit used to quantify the L-tryptophan containing L-tryptophan oxidase, and an enzyme sensor using said L-tryptophan oxidase. This method, kit and enzyme sensor use an L-tryptophan-specific enzyme, so are capable of quantifying L-tryptophan even in the present of other amino acids.

IPC Classes  ?

• C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
• C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12N 15/09 - Recombinant DNA-technology

Abstract

Disclosed is a microwell array chip which has a microwell layer having a plurality of microwells on the surface of a substrate, is shaped and dimensioned to accommodate only one biological cell per microwell, and has a magnetic film on the bottom surface of the microwells, there being no magnetic member other than the magnetic film, and the surface of the magnetic film and the surface of the microwell layer having a multilayer film composed of a light-shielding film and a silica film or a parylene film. Also disclosed is a method for accommodating specimen cells in the microwells of the microwell array and recovering target cells. This cell chip is capable of accommodating cells in a sample efficiently and in a short period of time, and is suitable for use in cell screening performed by observation of the fluorescence of a fluorescently labeled antigen for binding to a protein on the chip surface. The use of the microwell array chip to observe the fluorescence of the fluorescently labeled antigen on the chip surface makes it possible to specifically and effectively identify and recover target cells.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues

Abstract

A 4-HPPD inhibitor-resistant gene is identified as a gene (HIS1 gene) which is estimated as an iron-/ascorbic acid-dependent oxidoreductase gene located on the short arm of chromosome-2 of a rice plant, by carrying out QTL analysis using a 4-HPPD inhibitor-sensitive rice plant and a 4-HPPD inhibitor-resistant rice plant or the like. It is also found that a homologous gene (HSL1 gene) to the HIS1 gene is located on chromosome-6 of a rice plant. It is found that a plant having improved resistivity or sensitivity to a 4-HPPD inhibitor can be produced with high efficiency and the resistivity or sensitivity of a plant to a 4-HPPD inhibitor can be determined with high efficiency using the aforementioned genes.

IPC Classes  ?

• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 1/00 - Processes for modifying genotypes
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

[Problem] In order to provide a bonding member which is light weight and has a superior ability to bond a magnesium member and an aluminium member, and a manufacturing method for the bonding member which enables the simple, high productivity manufacture of said bonding member. [Solution] The present invention is a bonding member (100) provided with a magnesium member (1) comprising a magnesium alloy, an aluminium member (2) comprising an aluminium alloy, and an intermediate layer (3) formed between the magnesium member (1) and the aluminium member (2); wherein the intermediate layer (3) comprises an insert material selected from a group comprising Ni, Cu, and Ti, and the magnesium member (1), the aluminium member (2), and intermediate layer (3) are integrally bonded.

IPC Classes  ?

• B32B 15/01 - Layered products essentially comprising metal all layers being exclusively metallic
• B23K 20/00 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating
• B23K 20/02 - Non-electric welding by applying impact or other pressure, with or without the application of heat, e.g. cladding or plating by means of a press

Abstract

Disclosed is a means for providing a biosensor chip which is composed of a two-part structure that contains a metal electrode having good electrical conductivity and can be formed by injection molding. The means provides a biosensor chip, which is reduced in dimensional variations, by bonding the two components of the two-part structure under such conditions that do not affect the activity of an enzyme or the like. Specifically disclosed is a kit for biosensor chip assembly, which is composed of: a first member (10) that comprises a first electrode (12), a first wiring line (13) and a first bonding surface (14); and a second member (20) that comprises a second electrode (22), a second wiring line (23) and a second bonding surface (24). The kit for biosensor chip assembly is capable of assembling a biosensor by arranging and bonding the first member (10) and the second member (20) such that the first bonding surface (14) and the second bonding surface (24) face each other. A space, into which a subject that is a measurement object is introduced, and an introduction hole (16), through which the subject is introduced into the space, are provided between the first electrode (12) and the second electrode (22) that are arranged to face each other by the bonding. Also specifically disclosed are: a method for producing a biosensor chip using the kit; and a biosensor chip that is produced using the kit.

IPC Classes  ?

• G01N 27/28 - Electrolytic cell components
• G01N 27/327 - Biochemical electrodes
• G01N 27/416 - Systems

Abstract

Disclosed are: an enzymatic quantitative determination method which can determine the quantity of taurine in a sample at lower cost and more readily compared with conventional methods; and a determination kit and an enzyme sensor, both of which can be used in the enzymatic quantitative determination method. Specifically disclosed are: a method for determining taurine contained in a sample, which comprises reacting the sample with taurine dioxygenase and determining the quantity of a product of the reaction; an enzyme sensor characterized by comprising taurine dioxygenase; and a taurine determination kit comprising the following reagents (1) to (3): (1) taurine dioxygenase; (2) bivalent iron and α-ketoglutaric acid; and (3) a reagent for detecting a product produced by a reaction with taurine dioxygenase.

IPC Classes  ?

• C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12Q 1/32 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving dehydrogenase
• G01N 27/327 - Biochemical electrodes
• C12N 15/09 - Recombinant DNA-technology

Abstract

A method for analyzing L-threonine contained in an analyte, which comprises mixing a sample containing the analyte with an L-threonine dehydrogenase derived from Cupriavidus necator and a coenzyme NAD+ and analyzing the amount of NADH or 2-amino-3-oxobutyric acid after a predetermined period; an L-threonine dehydrogenase derived from Cupriavidus necator, which is a novel L-threonine dehydrogenase (TDH; EC 1.1.1.103) and can be utilized in the above-mentioned analysis method; a method for preparing a gene or the like to be used in the preparation of the enzyme, or a method for preparing the enzyme; an L-threonine analysis kit comprising (A) the L-threonine dehydrogenase and (B) a coenzyme NAD+; an enzyme preparation for use in the analysis of L-threonine, which comprises the L-threonine dehydrogenase contained in a buffer solution; and an enzyme sensor utilizing the L-threonine dehydrogenase.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12Q 1/32 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving dehydrogenase
• G01N 27/327 - Biochemical electrodes

Abstract

In order to provide a new method for producing a glucuronic acid conjugate, said method having excellent production characteristics and replacing methods using Saccharomyces pombe, and to provide a new means used in this production method, disclosed are: a transformed Saccharomyces cerevisiae wherein a gene coding for a UDP-glucose dehydrogenase and a gene coding for a UDP-glucose transferase are inserted in a manner such that said genes can be expressed; a transformed Saccharomyces cerevisiae wherein a gene coding for a cytochrome P450 gene is also inserted in a manner such that said gene can be expressed; and a method for producing a glucaronic acid conjugate that includes culturing transformed Saccharomyces cerevisiae in the presence of glucose and a substance to be conjugated, generating the glucuronic acid conjugate of the aforementioned substance to be conjugated.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

Abstract

Disclosed is a method for producing (S)-1,1,1-trifluoro-2-propanol with high optical purity and high yield by having at least one kind of microorganism, which is selected from the group consisting of Hansenula polymorpha, Pichia anomala, Candida parapsilosis, Candida mycoderma, Pichia naganishii, Candida saitoana, Cryptococcus curvatus, Saturnospora dispora, Saccharomyces bayanus and Pichia membranaefaciens, act on 1,1,1-trifluoroacetone. Since microorganisms found in nature are made to act in a natural state, the problems to be raised when a transformant or the like is used can be avoided in this method. Consequently, the method can be easily put in industrial practice.

IPC Classes  ?

• C12P 7/04 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic

Abstract

Provided is an anisotropically shaped powder that is ideal as a plate-like crystal used in the production of a niobate-based KNNbO3-NaNbO3-LiNbO3 crystal oriented ceramic and the like, and further provided is a method for producing the anisotropically shaped powder. The method for producing the anisotropically shaped powder comprises hydrothermal synthesis by adding an oxide powder such as Nb2O5 and a surfactant to an aqueous solution of an alkali hydroxide such as NaOH or KOH, washing the resulting reaction product with an organic solvent, and further baking the washed product at 170ºC to 700ºC. The anisotropically shaped powder obtained according to this production method has a pseudo-cubic perovskite structure in which the crystal face is oriented in the (100) plane and the ratio of the average grain length in the major axis direction and the average grain length in the thickness direction is 2 to 20.

IPC Classes  ?

• C01G 33/00 - Compounds of niobium
• C04B 35/626 - Preparing or treating the powders individually or as batches
• H01L 41/187 - Ceramic compositions

Abstract

Disclosed are: an enzymatic quantification method which can quantify L-lysine in a biological sample specifically even when other amino acid co-exists in the biological sample; and a measurement kit and an enzyme sensor, both of which can be utilized in the practice of the enzymatic quantification method. Specifically disclosed are: a method for measuring L-lysine contained in a biological sample, which comprises allowing an L-lysine ε-oxidase to act on an analyte containing the biological sample and quantifying a product produced by the action of the L-lysine ε-oxidase; an enzyme sensor comprising an L-lysine ε-oxidase; and a kit for measuring L-lysine, which comprises the following reagents: (1) an L-lysine ε-oxidase; and (2) a reagent capable of detecting a product produced by the action of the L-lysine ε-oxidase.

IPC Classes  ?

• G01N 27/416 - Systems
• C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
• C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
• C12Q 1/28 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving peroxidase
• C12Q 1/32 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving dehydrogenase
• G01N 27/327 - Biochemical electrodes
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

Disclosed is a hydroelectric power generation device provided with: a water intake cylinder (14), through which water passes from the top to the bottom, having a vertical central axis; a water funnel (15) provided below the water intake cylinder (14) around the same axis; fixed guide plates (20) that are provided inside the water funnel (15) and cause water to flow in a constant direction; and a rotating screw (22), below the fixed guide plates (20), that receives water flowing from the fixed guide plates (20) and rotates. A generator (40) is coupled to the rotating shank (24) of the rotating screw (22). The water funnel (15) comprises an outer water funnel tube (17) having a diameter that decreases downwards, and a water funnel drum (16) that is provided inside the outer water funnel tube (17) around the same axis and has a diameter that increases downwards. The water funnel (15) decreases the cross-sectional area of the flow path and the fixed guide plates (20) cause the water flow to hit the blade (22a) surfaces of the rotating screw (22) perpendicularly.

IPC Classes  ?

• F03B 3/12 - BladesBlade-carrying rotors

Abstract

High-intensity and high-efficient ultraviolet light is generated by applying polyphase alternating current discharge plasma in a multipole magnetic field to a light source for generating ultraviolet light and using mercury and general molecular gas other than rare gas. The interior of a planar container (3) is evacuated, and 1 Torr or less of molecular gas used for discharge light emission is filled or poured thereinto. A 12-phase alternating current power supply of 1 kw or less is connected to 12 split electrodes (1) and discharge electric energy is supplied thereto. Thus, plasma (P) by stable alternating-current glow discharge is generated along the surface of the split electrodes (1) covered with a barrier layer (2). As a result of discharge, light with a wavelength unique to molecular gas which contains ultraviolet light is emitted and extracted outward from a light extraction window (32).

IPC Classes  ?

• H01J 65/00 - Lamps without any electrode inside the vesselLamps with at least one main electrode outside the vessel
• H01J 61/12 - Selection of substances for gas fillingsSpecified operating pressure or temperature
• F21V 9/08 - Elements for modifying spectral properties, polarisation or intensity of the light emitted, e.g. filters for producing coloured light, e.g. monochromaticElements for modifying spectral properties, polarisation or intensity of the light emitted, e.g. filters for reducing intensity of light
• F21V 9/16 - Selection of luminescent materials for light screens

Abstract

A hydraulic power generator comprises a cylindrical water intake drum (14) the axial direction of which is positioned in the vertical direction and through which water flows downward, a guide member (15) which is provided in the water intake drum (14) and allows the water to flow in a specified direction, and a rotary screw (22) provided below the guide member (15) and rotated by receiving the water flown from the guide member (15). The guide member (15) includes a water throttling drum (16) positioned on the center axis of the water intake drum (14) and having a diameter gradually increased in downward direction, and a fixed screw (20) which partitions the space between the water throttling drum (16) and the water intake drum (14) along the circumference of the water intake drum (14). The mounting angle of the fixed screw (20) is made to be tilted relative to the axial direction of the water intake drum (14), and to intersect the surfaces of the rotary screw (22) that receive water.

IPC Classes  ?

• F03B 3/18 - Stator bladesGuide conduits or vanes, e.g. adjustable
• F03B 17/06 - Other machines or engines using liquid flow, e.g. of swinging-flap type

Abstract

A variable-shape mattress is provided with a bag (12) having placed therein powder particles or granules with a mixed substance, to which liquid is added, located therebetween, and also with a vibrator (14) for vibrating the bag (12). The bag (12) is adapted such that, even if that surface portion of the bag (12) which is in contact with a supported body supported on the bag (12) is subjected to the load of the supported body, owing to the mixed substance, the surface portion is less likely to deform. The vibrator (14) is composed of a supporting spring (18), a motor (22), and a weight (22c) eccentrically mounted to the rotating shaft (22b) of the motor (22). When the vibrator (14) vibrates, the mixed substance is fluidized according to the load and shape of the supported body such as a human body, which deforms the surface portion in contact with the supported body. When the vibration of the vibrator (14) stops, the shape of the surface portion of the bag (12) is fixed.

IPC Classes  ?

• A61G 7/05 - Parts, details or accessories of beds
• A47C 27/00 - Spring, stuffed or fluid mattresses specially adapted for chairs, beds or sofas

Abstract

It is intended to provide a method whereby the responses of lymphocytes to stimuli of more than 10,000 antigens held on a chip can be simultaneously measured and the states of the individual cells can be understood, and a means to be used in this method. A microwell array having a plural number of wells on one of the main surfaces of a substrate wherein each well has such a size as allowing the entrance of a single cell alone. In the vicinity of the wells on the main surface, a coating layer comprising a substance, which is capable of binding to a product produced by the cells stored therein, is formed. A method of screening target cells which comprises: storing individual cells in sample cells together with a liquid culture medium in the wells of the microwell array as described above; dipping the coating layer and the wells in the liquid culture medium; thus culturing the cells in the state where the substances contained in the liquid culture medium can diffuse from the wells into the coating layer; thus supplying a labeling agent, which is capable of binding specifically to a substance produced by the target cells involved in the sample cells, to the coating layer; and then detecting the substance produced by the target cells, which has been bound to the substance in the coating layer, based on the labeling agent to thereby specify the target cells.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/18 - Multiple fields or compartments
• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
• G01N 33/15 - Medicinal preparations
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

Disclosed is a method for the analysis of L-methionine contained in a sample, which comprises the steps of: subjecting the sample to a treatment that enables the conversion of at least a part of a branched amino acid into an oxo acid; incubating the treated sample together with a modified phenylalanine dehydrogenase and a reaction solution containing resazurin, diaphorase and reduced nicotinamide adenine dinucleotide (NAD+); and detecting the color developed in the reaction solution after the incubation. Also disclosed is a method for reducing the amount of a branched amino acid in a sample, wherein the branched amino acid is one that is often contained in a test sample for the analysis of L-methionine, and which comprises the step of converting at least a part of the branched amino acid into an oxo acid. It becomes possible to provide a highly sensitive and convenient method for the analysis of L-methionine contained in a sample by using a modified phenylalanine dehydrogenase having the substrate specificity for L-methionine and also provide a method for reducing measurement errors caused by an amino acid other than L-amino acid occurring in a blood sample.

IPC Classes  ?

• C12Q 1/32 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving dehydrogenase
• C12N 15/09 - Recombinant DNA-technology
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
• G01N 21/78 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour

Abstract

A modified enzyme having such modification of at least three amino acid residues that the modified enzyme can have improved substrate specificity of phenylalanine dehydrogenase (EC 1.4.1.20) for methionine; DNA encoding the modified enzyme; a method for the preparation of a modified enzyme which has such modification of at least three amino acid residues that the modified enzyme can have improved substrate specificity of phenylalanine dehydrogenase (EC 1.4.1.20) for methionine, comprising the steps of culturing a transformant transformed with a vector carrying the DNA, and collecting the modified enzyme from the culture; and a method for the analysis of L-methionine contained in a sample by using the modified enzyme or protein. A phenylalanine dehydrogenase having a substrate specificity for L-methionine can be produced by an evolutionary molecular engineering approach to provide a function-modified amino acid dehydrogenase suitable for enzymatic fluorometry of methionine. It becomes possible to provide a method for the analysis of L-methionine contained in a sample (a blood sample) by using the function-modified amino acid dehydrogenase.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C07K 19/00 - Hybrid peptides
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12Q 1/32 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase involving dehydrogenase

Abstract

[PROBLEMS] To provide a simple process for production of epitheaflagallin and epitheaflagallin-3-O-gallate which can be used in a food directly, and a process for production of a beverage containing epitheaflagallin and epitheaflagallin-3-O-gallate by utilizing the above-mentioned process. [MEANS FOR SOLVING PROBLEMS] Disclosed is a process for production of an epitheaflagallin compound, which comprises reacting epigallocathechin and/or epigallocathechin gallate with polyphenol oxidase in the presence of gallic acid to convert the epigallocathechin and/or epigallocathechin gallate into epitheaflagallin and/or epitheaflagallin-3-O-gallate, respectively. Also disclosed is a process for production of a beverage containing an epitheaflagallin compound, which comprises adding gallic acid to a tea leaf extract and reacting the mixture with polyphenol oxidase to convert at least a part of epigallocathechin and/or epigallocathechin gallate contained in the tea leaf extract into epitheaflagallin and/or epitheaflagallin-3-O-gallate, respectively.

IPC Classes  ?

• C12P 17/06 - Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
• A23F 3/14 - Tea preparations, e.g. using additives
• A23F 3/16 - Tea extractionTea extractsTreating tea extractMaking instant tea
• A23L 2/38 - Other non-alcoholic beverages
• A23L 2/52 - Adding ingredients

Abstract

⏧PROBLEMS] To provide a method of examining types of pathogenic bacteria including EHEC and bacteria belonging to the genus Legionella depending on polymorphisms in the base sequences that can be stored as text data. ⏧MEANS FOR SOLVING PROBLEMS] A method of examining types of pathogenic bacteria. Single nucleotide polymorphisms contained in a plural number of base sequence regions, which are highly homologous with each other, in the genes of the pathogenic bacteria are identified and the genotypes are determined based on the single nucleotide polymorphism data thus obtained. The above-described pathogenic bacteria belong to enterohemorrhagic Escherichia coli and the highly homologous base sequence regions as described above occur in the prophage genomic sequences of the E. coli bacteria. The above-described pathogenic bacteria belong to the genus Legionella.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 15/09 - Recombinant DNA-technology
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage

Abstract

[PROBLEMS] To provide a novel chip useful for tretaing cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. [MEANS FOR SOLVING PROBLEMS] A chip comprising a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing a chip which comprises a crosslinked product of a temperature-responsive polymer as a constituting member and is provided with a film having a hole pattern on the surface of the baseboard. This method comprises applying a composition containing a crosslinkable temperature-responsive polymer, a composition containing a crosslinkable temperature-responsive polymer and a crosslinking agent or a composition containing a temperature-responsive polymer and a crosslinking agent on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12N 5/06 -

Abstract

A microwell array chip comprising microwells in one major surface of a substrate. Each microwell has such a shape and dimensions that it contains only one living cell. On the major surface of the substrate where the openings of the microwells are provided, markers for the microwells are provided. Another microwell array chip comprising microwells in one major surface of a substrate. Each microwell has such a shape and dimensions that it contains only one living cell. A projection portion narrowing the opening of each microwell is disposed in the opening. A method for manufacturing the microwell array chip comprises a step of forming a film at least on one major surface of a substrate, a step of applying a resist to the formed film, a step of exposing the resist surface through a mask having a microwell pattern and removing the uncured portion of the resist, a step of making a hole of microwell array shape by etching the film and the exposed portion of the substrate, and a step of removing the resist. Further, another microwell array chip of a silicon having microwells in each of which one living cell of a subject is contained. Each microwell has such a shape and dimensions that it contains only one living cell.

IPC Classes  ?

• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 37/00 - Details not covered by any other group of this subclass