Abstract

According to the present disclosure, there is provided a system for culturing animal cells using a component derived from an organism such as algae as a nutrient source, and reusing the culture waste liquid. The present disclosure provides an organism or a cultured cell which undergoes a modification, in which the modification includes imparting or enhancing L-lactate utilization ability in the organism or the cultured cell. In addition, the present disclosure provides a method for culturing at least two types of cells including a cell X and a cell Y in a circulation manner, the method including: a step (a) of providing a component excreted from the cell X to the cell Y; a step (b) of providing a component derived from the cell Y to the cell X; a step (c) of culturing the cell X and the cell Y; and a step (d) of repeating the steps (a) to (c) as necessary, in which at least one of the components excreted from the cell X is an assimilable component of the cell Y, and at least one of the components derived from the cell Y is a nutritional component of the cell X.

IPC Classes  ?

• C12P 7/56 - Lactic acid
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12N 1/12 - Unicellular algaeCulture media therefor
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12R 1/89 - Algae

Abstract

An immunoisolation device that reduces diffusion distance, which is effective for increasing the permeability of substances such as physiologically active substances and nutrients, and has improved durability to withstand long-term transplantation. The immunoisolation device includes a sheet-like cell aggregate containing cells and an extracellular matrix, and an immunoisolation layer that covers the cell aggregate.

IPC Classes  ?

• A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof
• A61L 27/52 - Hydrogels or hydrocolloids
• A61L 27/56 - Porous or cellular materials
• C08L 1/02 - CelluloseModified cellulose
• C08L 23/0861 - Saponified copolymers, e.g. ethene vinyl alcohol copolymers

Abstract

The present invention provides an algae culturing method in which a composition containing a medium that has been used to culture animal cells is used as a medium. The present invention further provides a composition for culturing algae, the composition containing a medium that has been used to culture animal cells. Moreover, the present invention provides an algae and animal cell recycle culturing method.

IPC Classes  ?

• C12N 1/12 - Unicellular algaeCulture media therefor
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells

Abstract

The present invention provides a method for producing a composition for culturing animal cells, wherein the method includes: (1) a step in which an algae is mixed with a solid acid catalyst and an algae extract is obtained by heating; and (2) a step in which the algae extract is added to a medium for culturing animal cells and the concentration of the algae extract is adjusted. The present invention also provides a recycling/culturing method for algae and animal cells including: (i) a step in which a waste liquid (a first waste liquid) previously used to culture animal cells is used to culture algae; (ii) a step in which the algae is collected, mixed with a solid catalyst, and heated, and an algae extract is thereby obtained; (iii) a step in which the algae extract is added to the waste liquid (a second waste liquid) previously used to culture algae in (i), the concentration of the algae extract is adjusted, and a composition for culturing animal cells is produced; and (iv) a step in which animal cells are cultured using the composition for culturing animal cells.

IPC Classes  ?

• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12N 1/12 - Unicellular algaeCulture media therefor
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor

Abstract

Provided is a culture substrate which comprises, on a metal substrate that has undergone oxygen plasma processing and silanization processing, a temperature-responsive polymer having a glass transition temperature of greater than 121°C.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C08F 220/56 - AcrylamideMethacrylamide
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues

Abstract

A plasma exchanging system 100 is provided with: an oxygenator 20 that adds oxygen to a liquid for adding oxygen to blood; a plasma separator 10 having a hollow fiber membrane through which plasma migration from blood to the liquid, oxygen migration from the liquid to blood, and carbon dioxide migration from blood to the liquid occur; and a fractionator 30 that fractionates plasma included in the liquid.

IPC Classes  ?

• A61M 1/36 - Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation
• A61M 1/18 - Dialysis systemsArtificial kidneysBlood oxygenators with membranes in the shape of hollow fibres
• A61M 1/32 - Oxygenators without membranes

Abstract

[Problem] The purpose is to provide a novel method for culturing myoblasts or skeletal muscle stem cells. [Solution] The method for culturing myoblasts or skeletal muscle stem cells of the present invention includes a step that cultures myoblasts or skeletal muscle stem cells in a medium containing the culture supernatant of epithelial cells.

IPC Classes  ?

• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• C12N 5/0775 - Mesenchymal stem cellsAdipose-tissue derived stem cells

Abstract

The problem to be solved by the present invention is to provide: an immunoisolation device which can achieve both of the reduction in diffusion distance effective for the improvement in permeability of a substance such as a physiologically active substance and a nutrient and the improvement in durability for withstanding transplantation for a long period of time; and an immunological control technology using a cell sheet. The present invention provides an immunoisolation device provided with a sheet-like cell mass containing cells and an extracellular matrix and an immunoisolation layer that covers the cell mass.

IPC Classes  ?

• A61L 27/16 - Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
• A61L 27/20 - Polysaccharides
• A61L 27/22 - Polypeptides or derivatives thereof
• A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof
• A61L 27/38 - Animal cells
• A61L 27/52 - Hydrogels or hydrocolloids

Abstract

S100A8-inhibiting peptides include (A) a peptide of 5 to 10 residues in length containing a fifth alanine (Ala) from an N-terminus in an amino acid sequence of SEQ ID NO: 1, or (B) a peptide consisting of an amino acid sequence of SEQ ID NO: 2.

IPC Classes  ?

• C07K 7/06 - Linear peptides containing only normal peptide links having 5 to 11 amino acids
• A61K 38/08 - Peptides having 5 to 11 amino acids
• A61P 35/04 - Antineoplastic agents specific for metastasis

Abstract

A drill stopper (1) comprises: a body (2) having a through-hole (2a) through which a drill shaft penetrates; and a lever (3) supported by the body (2) such that the lever (3) can rotate about a predetermined rotation axis between a fixation position at which the body (2) is fixed to the drill shaft inside the through-hole (2a) and a fixation releasing position at which the body (2) is released from the drill shaft. The lever (3) has an abutting part (3c) abutting, in the direction along the central axis (O) of the through-hole (2a), against another member attached to the drill shaft. The abutting part (3c) is disposed at a position apart, in the direction along the central axis (O), from the body (2) in the state where the lever (3) is placed at the fixation releasing position.

IPC Classes  ?

• A61B 17/16 - Instruments for performing osteoclasisDrills or chisels for bonesTrepans
• A61B 17/56 - Surgical instruments or methods for treatment of bones or jointsDevices specially adapted therefor

Abstract

The present disclosure provides a system for culturing animal cells by using, as a source of nutrient, components derived from a living organism such as algae, and for reutilizing a waste culture liquid obtained therefrom.　The present disclosure provides a living organism or a cultured cell, which has undergone a modification. Said modification includes providing or enhancing the ability to utilize L-lactic acid in the living organism or the cultured cell. The present disclosure also provides a method for culturing, in recycling-oriented manner, at least two types of cells, which are cell X and cell Y, the method comprising: a step (a) for providing components excreted from the cell X to the cell Y; a step (b) for providing components originating from the cell Y to the cell X; a step (c) for culturing the cell X and the cell Y; and, as necessary, a step (d) for repeating steps (a)-(c). At least one of the components excreted from the cell X is a component that can be utilized by the cell Y. At least one of the components originating from the cell Y is a nutritional component for the cell X.

IPC Classes  ?

• C12P 13/04 - Alpha- or beta-amino acids
• C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• C12N 5/07 - Animal cells or tissues
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 1/12 - Unicellular algaeCulture media therefor
• C12N 1/13 - Unicellular algaeCulture media therefor modified by introduction of foreign genetic material
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/53 - Oxidoreductases (1)
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12P 7/56 - Lactic acid
• C12P 7/62 - Carboxylic acid esters
• A23L 13/00 - Meat productsMeat mealPreparation or treatment thereof

Abstract

The present invention provides a method for producing a composition for culturing animal cells, wherein the method includes: (1) a step in which an algae is mixed with a solid acid catalyst and an algae extract is obtained by heating; and (2) a step in which the algae extract is added to a medium for culturing animal cells and the concentration of the algae extract is adjusted. The present invention also provides a recycling/culturing method for algae and animal cells including: (i) a step in which a waste liquid (a first waste liquid) previously used to culture animal cells is used to culture algae; (ii) a step in which the algae is collected, mixed with a solid catalyst, and heated, and an algae extract is thereby obtained; (iii) a step in which the algae extract is added to the waste liquid (a second waste liquid) previously used to culture algae in (i), the concentration of the algae extract is adjusted, and a composition for culturing animal cells is produced; and (iv) a step in which animal cells are cultured using the composition for culturing animal cells.

IPC Classes  ?

• C12N 5/07 - Animal cells or tissues
• C12N 1/12 - Unicellular algaeCulture media therefor

Abstract

The present invention provides an algae culturing method in which a composition containing a medium that has been used to culture animal cells is used as a medium. The present invention further provides a composition for culturing algae, the composition containing a medium that has been used to culture animal cells. Moreover, the present invention provides an algae and animal cell recycle culturing method.

IPC Classes  ?

• C12N 1/12 - Unicellular algaeCulture media therefor
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor

Abstract

The present invention provides a production method for a composition for cell culturing. This production method comprises: (1) a step for subjecting algae to an acid hydrolysis treatment and/or an alkali hydrolysis treatment; (2) a step for neutralizing the hydrolysis product obtained in the step (1) to obtain an algae extract; and (3) a step for mixing the algae extract with a medium for cell culturing, wherein the medium for cell culturing does not substantially contain L-glutamine.

IPC Classes  ?

• C12N 1/12 - Unicellular algaeCulture media therefor
• C12N 1/06 - Lysis of microorganisms

Abstract

Disclosed is a method for acquiring information on spinal muscular atrophy, comprising acquiring a fluorescence image of a nucleated cell in a measurement sample, wherein the measurement sample is a sample prepared from a blood specimen obtained from a subject, an SMN protein in the nucleated cell is labeled with a first fluorescent dye, and a predetermined nuclear protein in the nucleated cell is labeled with a second fluorescent dye, acquiring an intracellular distance between a first bright spot corresponding to the first fluorescent dye and a second bright spot corresponding to the second fluorescent dye in the fluorescence image, and acquiring a value regarding a number of nucleated cells in which the intracellular distance is equal to or less than a first threshold value, wherein the value is an indicator of spinal muscular atrophy affection.

IPC Classes  ?

• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
• G01N 21/64 - FluorescencePhosphorescence

Abstract

A S100A8-inhibiting peptide comprises (A) a peptide which comprises a portion of the amino acid sequence represented by SEQ ID NO: 1, contains an alanine (Ala) residue located at position-5 from the N-terminal of the aforementioned amino acid sequence, and has a length composed of 5 to 10 residues or (B) a peptide which comprises the amino acid sequence represented by SEQ ID NO: 2.

IPC Classes  ?

• C07K 7/06 - Linear peptides containing only normal peptide links having 5 to 11 amino acids
• A61K 38/08 - Peptides having 5 to 11 amino acids
• A61P 1/04 - Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• A61P 3/00 - Drugs for disorders of the metabolism
• A61P 17/00 - Drugs for dermatological disorders
• A61P 25/18 - Antipsychotics, i.e. neurolepticsDrugs for mania or schizophrenia
• A61P 29/00 - Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agentsNon-steroidal antiinflammatory drugs [NSAID]
• A61P 31/14 - Antivirals for RNA viruses
• A61P 35/04 - Antineoplastic agents specific for metastasis
• A61P 37/08 - Antiallergic agents
• A61P 43/00 - Drugs for specific purposes, not provided for in groups
• C07K 19/00 - Hybrid peptides

Abstract

A conventional example (FFR-CT) regarding measurement of coronary fractional flow reserve (FFR) from a coronary artery 3D model by a simulation based on numerical fluid dynamics, has limitations such as the inability to perform analysis of a subject having coronary artery calcification or stent placement. In addition, FFR-CT is merely a calculation based on static information, and the hardness and the distensibility of the coronary artery wall are not taken into account.　The present invention provides a functional ischemia detection system or the like capable of calculating instantaneous flow reserve iFR (in the present invention, CT-iFR) and non-invasively detecting functional ischemia, by: reconstructing, by using a predictive interpolation technique, multi-time phase CCTA data obtained by coronary artery contrast CT; obtaining temporal concentration curves of a coronary artery proximal portion and a coronary artery distal portion from the reconstructed CCTA data; and quantifying coronary artery flow using a mathematical model (a maximum slope method, a convolution integral method, or the like).

IPC Classes  ?

• A61B 6/03 - Computed tomography [CT]

Abstract

The objective of the present invention is to improve the quality and strength of a tubular cell structure produced only from cells. The representative configuration of the method for culturing a tubular cell structure according to the present invention is characterized in that cells are formed into a tubular cell structure, and the cell structure is cultured while expanding the cell structure from the lining of the cell structure by a balloon toward the outside in the radial direction.

IPC Classes  ?

• A61F 2/06 - Blood vessels
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor

Abstract

[Problem] To provide an automated surgery planning system which can reduce a physician's load by eliminating manual setting of anatomical landmarks in medical image data. [Solution] According to one aspect of the present embodiment, an automated surgery planning system is provided. The automated surgery planning system is configured so as to execute the following steps. In a readout step, medical image data in which a subject's bone structure can be retained or reproduced as information is read out. In a specification step, the medical image data is input into a pre-stored machine learning model to extract at least one anatomical landmark in the medical image data, thereby specifying the location of the anatomical landmark in the subject's bone structure.

IPC Classes  ?

• A61B 6/03 - Computed tomography [CT]
• A61B 6/14 - Applications or adaptations for dentistry

Abstract

The present invention is a surgery-assisting device characterized by comprising: a sleeve provided with a proximal-end linear part, a distal-end linear part, and a bent part which is arcuate in side view and which is provided between the proximal-end linear part and the distal-end-side linear part; an arc track rail for retaining the bent part so that the bent part can slide along the direction of the arc shape thereof; a rail-retaining arm for retaining the arc track rail; a rotating bearing for supporting the rail-retaining arm while allowing the rail-retaining arm to rotate; and an operating arm which extends into the sleeve, the proximal-end linear part and the distal-end linear part being positioned on a substantially straight line, and the center of curvature of the bent part being on a line of extension of the proximal-end linear part and the distal-end linear part and on the rotational axis of the rotating bearing.

IPC Classes  ?

• A61B 90/50 - Supports for surgical instruments, e.g. articulated arms
• A61B 17/29 - Forceps for use in minimally invasive surgery
• A61B 17/94 - Endoscopic surgical instruments

Abstract

The present invention provides a production method for a composition for cell culturing. This production method comprises: (1) a step for subjecting algae to an acid hydrolysis treatment and/or an alkali hydrolysis treatment; (2) a step for neutralizing the hydrolysis product obtained in the step (1) to obtain an algae extract; and (3) a step for mixing the algae extract with a medium for cell culturing, wherein the medium for cell culturing does not substantially contain L-glutamine.

IPC Classes  ?

• C12N 5/07 - Animal cells or tissues
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 1/12 - Unicellular algaeCulture media therefor

Abstract

A sheet for covering a wound includes a laminate of a serosal membrane and a cell sheet. The sheet for covering a wound has a proper thickness and strength. The sheet does not flow out from the wound site and can be fixed to the wound site by suture or anastomosis, if necessary. The sheet can be stably engrafted onto a wound region.

IPC Classes  ?

• A61L 27/38 - Animal cells
• A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof

Abstract

The present invention provides a tubular tissue preparation device provided with: a chamber for accommodating a tubular support body; a first support part for communicating the inside and the outside of the chamber and fixing the tubular support body; and a pressurizing/depressurizing means for controlling the pressure in an inner space of the chamber, wherein the first support part is for fixing a first opening section of the tubular support body, communicating a lumen of the tubular support body and the outside of the chamber, and separating the lumen of the tubular support body and the inner space of the chamber. Also, the present invention provides a tubular tissue preparation kit that includes said device and a device for transporting sheet-shaped tissue. In addition, the present invention provides a tubular tissue preparation method using said device.

IPC Classes  ?

• B33Y 30/00 - Apparatus for additive manufacturingDetails thereof or accessories therefor
• B33Y 50/02 - Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
• B33Y 70/00 - Materials specially adapted for additive manufacturing
• B33Y 80/00 - Products made by additive manufacturing
• B29C 64/393 - Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters

Abstract

The present invention relates to a therapeutic substance delivery device for delivering a therapeutic substance to a desired site in a bodily duct, characterized in that the therapeutic substance delivery device is provided with: a therapeutic substance loading portion; a connector which is connected to the therapeutic substance loading portion; and a supplying/discharging pipe connected to the connector; and in that the therapeutic substance loading portion includes a main body portion in which a recessed portion is formed, a resilient film, and a connecting pipe; the connector is provided with a joint main body, a flange portion fixed to the other end portion side of the joint main body, and a fixing nut through which the joint main body passes; the joint main body is provided with a tube fastening portion; and at least part of the inner wall of the fixing nut is provided with a thread.

IPC Classes  ?

• A61B 17/00 - Surgical instruments, devices or methods

Abstract

Provided are: a therapeutic agent for urological cancer, particularly urological cancer with a reduced function of lysine (K)-specific demethylase 6A (KDM6A), which is characterized by inhibiting both IL-6 activity and CCR2/CCL2 activity; and a therapeutic method.

IPC Classes  ?

• A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
• A61K 45/06 - Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• A61P 13/00 - Drugs for disorders of the urinary system
• A61P 13/08 - Drugs for disorders of the urinary system of the prostate
• A61P 13/10 - Drugs for disorders of the urinary system of the bladder
• A61P 13/12 - Drugs for disorders of the urinary system of the kidneys
• A61P 35/00 - Antineoplastic agents
• A61P 43/00 - Drugs for specific purposes, not provided for in groups
• A61K 31/28 - Compounds containing heavy metals

Abstract

A cell culture container may include an inlet through which a fluid is supplied, an outlet through which a fluid is discharged, and a flow path configured to connect the inlet to the outlet and accommodate a cell culture substrate containing gold nanoparticles and capable of being denatured by heating.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells

Abstract

The presently disclosed subject matter is to provide a hepatocyte structure, particularly, a hepatocyte structure that mimics nonalcoholic steatohepatitis (NASH). The presently disclosed subject matter is to provide a hepatocyte structure, particularly, a hepatocyte structure that mimics nonalcoholic steatohepatitis (NASH). A hepatocyte construct including an aggregate containing hepatocytes and adherent cells that are non-hepatocytes, and wherein the hepatocytes include ballooned hepatocytes is provided. Further, a method for producing a hepatocyte construct, comprising: (i) forming an aggregate by aggregating a cell group comprising hepatocytes and adherent cells that are non-hepatocytes; and (ii) culturing the aggregate is provided.

IPC Classes  ?

• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12N 5/0775 - Mesenchymal stem cellsAdipose-tissue derived stem cells

Abstract

The purpose of the present invention is to enable preventing aging or extending the life of a human or nonhuman organism with a method other than restricting calories or administering metformin.　It is possible to prevent aging or extend the life of a human or nonhuman organism by administering an agent that contains A) a xanthine oxidase/xanthine dehydrogenase inhibitor.　It is further possible to prevent aging or extend the life of a human or nonhuman organism by combining A) the xanthine oxidase/xanthine dehydrogenase inhibitor and B) hypoxanthine or a compound that can be converted to hypoxanthine in the body, and administering these simultaneously or administering these as a mixed agent or kit agents.

IPC Classes  ?

• A61K 31/403 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
• A61K 31/415 - 1,2-Diazoles
• A61K 31/426 - 1,3-Thiazoles
• A61K 31/444 - Non-condensed pyridinesHydrogenated derivatives thereof containing further heterocyclic ring systems containing a six-membered ring with nitrogen as a ring hetero atom, e.g. amrinone
• A61K 31/519 - PyrimidinesHydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
• A61K 31/522 - Purines, e.g. adenine having oxo groups directly attached to the heterocyclic ring, e.g. hypoxanthine, guanine, acyclovir
• A61K 31/7076 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
• A61K 31/708 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid having oxo groups directly attached to the purine ring system, e.g. guanosine, guanylic acid
• A61K 45/00 - Medicinal preparations containing active ingredients not provided for in groups
• A61P 43/00 - Drugs for specific purposes, not provided for in groups

Abstract

The present invention provides a method for swiftly producing a layered cell sheet that is non-invasively obtained and is utilizable for transplantation, etc., the method including (1) a step of applying a centrifugal force to a first cell sheet on a temperature-responsive culture surface for a predetermined time in a temperature range from a lower critical solution temperature of the temperature-responsive culture surface to 45° C., (2) a step of further placing a second cell sheet on the first cell sheet, and (3) a step of applying a centrifugal force to the first cell sheet and the second cell sheet on the temperature-responsive culture surface for a predetermined time in the temperature range from the lower critical solution temperature to 45° C.; and also provides a layered cell sheet obtained by the method.

IPC Classes  ?

• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells

Abstract

The present invention provides a novel topical composition that can be easily manufactured and is capable of improving the state of bodily tissue, especially epithelial tissue such as the skin or mucosae. In particular, the present invention provides a topical composition containing extracellular vesicles derived from buccal epithelial cells.

IPC Classes  ?

• A61K 35/36 - SkinHairNailsSebaceous glandsCerumenEpidermisEpithelial cellsKeratinocytesLangerhans cellsEctodermal cells
• A61P 1/16 - Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• A61P 9/00 - Drugs for disorders of the cardiovascular system
• A61P 11/00 - Drugs for disorders of the respiratory system
• A61P 13/12 - Drugs for disorders of the urinary system of the kidneys
• A61P 17/00 - Drugs for dermatological disorders
• A61P 17/02 - Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like

Abstract

The present invention addresses the problem of producing a large quantity of myocardial cells with high efficiency. Provided is a method for producing myocardial cells, comprising the steps of: (1) subjecting iPC cells to spheroid culture using a culture substratum having multiple compartments so that multiple embryoid bodies can be spheroid-cultured in an individually separated state, thereby forming multiple embryoid bodies; and (2) subjecting the multiple embryoid bodies produced in step (1) to three-dimensional suspension culture to induce the differentiation of the multiple embryoid bodies into myocardial cells.

IPC Classes  ?

• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

As a conventional example of an electrocardiogram diagnostic support method, methods have been proposed in which the learning stage is divided into two stages and a two-stage learning model is generated, but such models learn binary classification models that output 'normal' or 'abnormal' and output only whether the waveform of the ECG image indicates an abnormality, so do not determine an actual diagnosis (disease name, sickness name, etc.). In the present invention, an electrocardiogram diagnostic device is provided which uses training data comprising diagnoses ('normal', or multiple sickness names (disease names)) attached to electrocardiogram images to learn a model that, in addition to 'normal', can output disease names through machine learning such as CNN, LSTM, etc. By giving electrocardiogram images obtained in an actual test to a model that has been learned, the electrocardiogram diagnostic device outputs a diagnosis ('normal', or disease names) that can be obtained by reading the electrocardiogram image.

IPC Classes  ?

• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients
• A61B 5/0452 - Detecting specific parameters of the electrocardiograph cycle
• G06N 3/08 - Learning methods
• G06T 7/00 - Image analysis

Abstract

One embodiment of the present disclosure is provided with: an information acquisition portion; an information storage portion; an event extraction section; a region setting section; and a display section. The information storage portion adds, to medical information, a time at which the information acquisition portion acquires the medical information from each of multiple medical devices used in a surgery, and a label for specifying the medical information. The display section causes a display device to display, as medical information, an image including a surgery site and at least one event extracted by the event extraction section in a time region set by the region setting section based on the time added to the medical information.

IPC Classes  ?

• G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
• G16H 20/40 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance relating to mechanical, radiation or invasive therapies, e.g. surgery, laser therapy, dialysis or acupuncture
• G06F 3/14 - Digital output to display device
• A61B 5/00 - Measuring for diagnostic purposes Identification of persons
• A61B 34/20 - Surgical navigation systemsDevices for tracking or guiding surgical instruments, e.g. for frameless stereotaxis

Abstract

This cell culture container is filled with a cell culture matrix (14) including: a gel layer which is formed of a gel (15) denatured by heating; and a gold fine particle layer (13) which is formed on one surface of the gel layer, wherein cells are cultured on the one surface side of the cell culture matrix or on the other surface side thereof. This cell culture container manufacturing method comprises: a step for filling the cell culture container with a gel denatured by heating; and a step for forming a gold fine particle layer on one surface of the gel. This cell acquisition method comprises: a step for selecting cells to be acquired from among cells placed in the cell culture container; a step for emitting light onto a cell culture matrix in the vicinity of the selected cells; and a step for collecting the selected cells.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/26 - Inoculator or sampler
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus

Abstract

The present invention provides an angiogenesis inhibitor containing as an active ingredient LYPD1 protein or a derivative thereof, a part thereof, or a vector expressing the same, or a cell expressing the same. The present invention also provides a screening method for angiogenesis inhibitors that enhance the expression of LYPD1 protein wherein the method includes (i) a step for treating a first cell by a test substance and culturing and (ii) a step for detecting the expression level of LYPD1 protein from the first cell and comparing with the level of LYPD1 protein of an untreated first cell.

IPC Classes  ?

• A61K 38/16 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
• A61K 35/33 - Fibroblasts
• A61P 35/00 - Antineoplastic agents
• A61P 43/00 - Drugs for specific purposes, not provided for in groups
• C12N 15/07 - Human cells
• C12Q 1/06 - Quantitative determination
• G01N 33/15 - Medicinal preparations
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals

Abstract

The present invention provides a tubular tissue preparation device provided with: a chamber for accommodating a tubular support body; a first support part for communicating the inside and the outside of the chamber and fixing the tubular support body; and a pressurizing/depressurizing means for controlling the pressure in an inner space of the chamber, wherein the first support part is for fixing a first opening section of the tubular support body, communicating a lumen of the tubular support body and the outside of the chamber, and separating the lumen of the tubular support body and the inner space of the chamber. Also, the present invention provides a tubular tissue preparation kit that includes said device and a device for transporting sheet-shaped tissue. In addition, the present invention provides a tubular tissue preparation method using said device.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• A61L 27/38 - Animal cells
• A61L 27/50 - Materials characterised by their function or physical properties
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues

Abstract

The present invention provides a method for predicting the onset of glioma in a subject. Specifically, the present invention provides a method for predicting the onset of glioma in a subject, wherein the method is characterized by including: (1) a step for detecting vimentin in a blood serum sample derived from the subject; and (2) a step for predicting, when vimentin is detected, that there is a high possibility that the subject suffers from glioma.

IPC Classes  ?

• G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

A device for measuring a tension of a sheet-like tissue containing cardiomyocytes includes a first gel adapter holder having a frame member and a first gel holding member protruding toward a part of an inside face of the frame member for fixing one end of a film-like gel; and a second gel adapter holder having a second gel holding member for fixing the other end of the gel and a connection member connected to the second gel holding member. A kit includes the tension measuring device; a substrate having a pair of gel molding protruding members fitted along the inside face of the frame member; and a gel forming lid body having a face parallel to a gel contact face of the substrate so as to form an upper face of the gel. Further, a system for measuring the tension includes the tension measuring device.

IPC Classes  ?

• G01N 13/02 - Investigating surface tension of liquids
• A61B 5/00 - Measuring for diagnostic purposes Identification of persons
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• B01L 9/00 - Supporting devicesHolding devices

Abstract

A cell culture container having a plurality of wells, wherein each well is filled with a cell culture substrate in which gold fine particles are dispersed in a gel that is denatured by heating. A method for obtaining a cell, including a step for selecting a cell to be obtained from cells disposed in wells of the cell culture container, a step for radiating light to a cell culture substrate filling a well of the cell culture container in which the cell to be obtained is disposed, a step for extracting the cell culture substrate from the cell culture container, and a step for recovering a cell from the gel extracted from the cell culture container.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 1/18 - Multiple fields or compartments
• C12N 5/07 - Animal cells or tissues

Abstract

Provided is a cell culture container equipped with an inlet (21) for injecting a fluid, an outlet (22) for discharging a fluid, and a flow path (23) that connects the inlet and the outlet and accommodates a cell culture substrate (14) obtained by dispersing gold microparticles (13) in a gel denatured by heating. Also provided is a method for acquiring cells, the method including a step for selecting cells to be acquired from cells cultured in the cell culture container, a step for irradiating the gel with light in the vicinity of the selected cells, a step for allowing a liquid to flow into the flow path of the cell culture container, and a step for recovering cells from the outlet of the cell culture container.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues

Abstract

The present invention provides a method for swiftly producing a layered cell sheet that is non-invasively obtained and is utilizable for transplantation, etc., the method including (1) a step of applying a centrifugal force to a first cell sheet on a temperature-responsive culture surface for a predetermined time in a temperature range from a lower critical solution temperature of the temperature-responsive culture surface to 45℃, (2) a step of further placing a second cell sheet on the first cell sheet, and (3) a step of applying a centrifugal force to the first cell sheet and the second cell sheet on the temperature-responsive culture surface for a predetermined time in the temperature range from the lower critical solution temperature to 45℃; and also provides a layered cell sheet obtained by the method.

IPC Classes  ?

• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• A61L 27/38 - Animal cells
• A61K 35/34 - MusclesSmooth muscle cellsHeartCardiac stem cellsMyoblastsMyocytesCardiomyocytes

Abstract

[Problem] To provide a composition having an estrogen-like action which comprises a hot water extract of Sparassis crispa mycelia.

IPC Classes  ?

• A61K 36/07 - Basidiomycota, e.g. Cryptococcus
• A23L 33/105 - Plant extracts, their artificial duplicates or their derivatives
• A61K 45/00 - Medicinal preparations containing active ingredients not provided for in groups
• A61P 3/06 - Antihyperlipidemics
• A61P 5/30 - Oestrogens
• A61P 9/10 - Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• A61P 43/00 - Drugs for specific purposes, not provided for in groups
• C12N 1/14 - Fungi Culture media therefor
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes

Abstract

Provided is a LYPD1 inhibitor for promoting vascular endothelial network formation in a biological tissue. Also provided is a medicinal composition, said medicinal composition comprising a LYPD1 inhibitor as an active ingredient, for treating and/or preventing neoangiogenic disorders. Also provided is a method which comprises: (a1) a step for providing a cell population containing first cells expressing LYPD1 and vascular endothelial cells; (a2) a step for treating the cell population obtained in step (a1) with a LYPD1 inhibitor; and (a3) a step for culturing the cell population obtained in step (a2).

IPC Classes  ?

• A61K 45/00 - Medicinal preparations containing active ingredients not provided for in groups
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 38/18 - Growth factorsGrowth regulators
• A61K 38/48 - Hydrolases (3) acting on peptide bonds (3.4)
• A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
• A61P 9/00 - Drugs for disorders of the cardiovascular system

Abstract

The present invention pertains to a method for culturing a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells at a temperature of 40.5° C. or higher and reducing the pluripotent stem cells included in the cell population. The present invention also pertains to a method for reducing pluripotent stem cells from a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells, wherein the method includes a step for activating the TRPV-1 expressed in the pluripotent stem cells included in the cell population. The present invention makes it possible to reduce the pluripotent stem cells remaining in an undifferentiated state when inducing the differentiation of a pluripotent stem cell population.

IPC Classes  ?

• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12N 5/074 - Adult stem cells
• C12N 15/09 - Recombinant DNA-technology
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

Provided is a puncturing instrument capable of administering (supplying) a liquid drug. The puncturing instrument comprises: a puncturing tip (2); a first tubular body (3) connected to the puncturing tip (2) at the distal end thereof; and an outside tubular body (5) covering at least part of the first tubular body (3). The first tubular body (3) is formed so as to be rotatable around a shaft in the longitudinal direction. The outer diameter of the first tubular body (3) is smaller than the inner diameter of the outside tubular body (5) and a liquid drug supply path (5a) is provided on the outside of the first tubular body (3).

IPC Classes  ?

• A61B 17/34 - TrocarsPuncturing needles

Abstract

One embodiment of the present disclosure is provided with: an information acquisition unit (30, 40-50); an information storage unit (30); an event extraction unit (72, S406); a region setting unit (72, S404); and a display unit (72, S408, S412, S416, S420, S424). The information storage unit adds, to medical information, a time at which the information acquisition unit acquires the medical information from each of a plurality of medical devices (102-112) used for surgery, and a label for specifying the medical information. The display unit causes a display device (74) to display, as medical information, an image including a surgery site and at least one event extracted by the event extraction unit in a time region set by the region setting unit on the basis of the time added to the medical information.

IPC Classes  ?

• A61B 34/20 - Surgical navigation systemsDevices for tracking or guiding surgical instruments, e.g. for frameless stereotaxis
• A61B 5/00 - Measuring for diagnostic purposes Identification of persons

Abstract

An object of the present invention is to obtain a thicker animal cell composition by a simple and less expensive method. Namely, an object of the present invention is to provide a method for culturing a thicker animal cell composition by eliminating the hypoxia associated with animal cell compositions, a method for producing an animal cell composition containing unicellular algae, and an animal cell composition. The present invention provides a method for culturing an animal cell composition in a culture medium in the presence of unicellular algae and under exposure to light. According to the method of the present invention, oxygen can be continuously supplied in the culture medium, cell damage is alleviated, and a thicker cell composition can be obtained in the absence of a capillary network.

IPC Classes  ?

• C12N 1/12 - Unicellular algaeCulture media therefor
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof
• A61L 27/38 - Animal cells

Abstract

A measuring apparatus relates to a contractile ability of a spontaneously pulsating myocardial tissue. The measuring apparatus includes a measurement table and a pressure sensor. The measurement table includes a storage section having an opening and configured to hold liquid inside the storage section, and an attaching portion on which the myocardial tissue is attached around the opening. The pressure sensor is disposed inside the storage section.

IPC Classes  ?

• G01N 33/483 - Physical analysis of biological material

Abstract

The purpose of the present invention is to provide a method capable of analyzing an SMN protein nuclear body that serves as a more highly reliable biomarker. The method according to the present invention is a method for analyzing the expression of an SMN protein nuclear body, and comprises the steps of: labeling at least one surface antigen marker for blood-derived nucleated cells with at least one labeling antibody in a sample containing the nucleated cells; labeling the SMN protein in the nucleated cells; labeling the nuclei of the nucleated cells; selecting one cell mass from among multiple cell masses of the nucleated cells, the multiple cell masses being cell masses in which the nucleus and the SMN protein are labeled and which have been classified on the basis of the surface antigen marker labeled with the labeling antibody or the like; and subjecting the selected cell mass to analysis on the expression of the SMN protein nuclear body on the basis of the labeling of the SMN protein. The method involves carrying out imaging flow cytometry using an objective lens at a specific magnification.

IPC Classes  ?

• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
• G01N 21/64 - FluorescencePhosphorescence
• G01N 33/48 - Biological material, e.g. blood, urineHaemocytometers
• G01N 33/49 - Physical analysis of biological material of liquid biological material blood
• G01N 33/536 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

The present invention addresses the problem of providing a cell sheet composition for healing or preventing discharge from a wounded region of a luminal organ. The present invention also addresses the problem of providing a method in which the cell sheet composition is affixed to a wounded region of a luminal organ to heal or prevent discharge from the wounded region of the luminal organ. The present invention provides a cell sheet composition including mesenchymal stem cells which is characterized by being affixed to a wounded region of a luminal organ in order to heal or prevent discharge from the wounded region of the luminal organ. The present invention also provides a method in which the cell sheet composition including mesenchymal stem cells is affixed to a wounded region of a luminal organ to heal or prevent discharge from the wounded region of the luminal organ.

IPC Classes  ?

• A61K 35/28 - Bone marrowHaematopoietic stem cellsMesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• A61K 35/51 - Umbilical cordUmbilical cord bloodUmbilical stem cells
• A61K 35/545 - Embryonic stem cellsPluripotent stem cellsInduced pluripotent stem cellsUncharacterised stem cells
• A61L 27/38 - Animal cells
• A61P 1/00 - Drugs for disorders of the alimentary tract or the digestive system
• A61P 1/04 - Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• A61P 41/00 - Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution

Abstract

The present invention provides a method for producing a layered cell sheet composition, the method including a step for bringing a cultured cell movement jig into contact with an upper surface of a sheet-like cell group so that a first cell sheet adheres to the cultured cell movement jig, a step for applying a cell adhesive substance to an upper surface of a second sheet-like cell group, and/or a lower surface of the first cell sheet adhered to the cultured cell movement jig, and a step for bringing the lower surface of the first cell sheet adhered to the cultured cell movement jig, into contact with the upper surface of the second sheet-like cell group so that a second cell sheet is adhered to the first cell sheet. The present invention also provides a layered cell sheet composition produced using the method. The present invention also provides a device for producing the layered cell sheet composition.

IPC Classes  ?

• C12N 5/07 - Animal cells or tissues
• A61L 27/38 - Animal cells
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 3/04 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing thin layers

Abstract

The present invention addresses the problem of providing a collagen production inhibitor, which comprises as an active ingredient a low-molecular compound having an effect of effectively inhibiting (preventing) collagen production, and a prophylactic or therapeutic agent for skin fibrotic diseases which is capable of effectively preventing or treating skin fibrotic diseases. A preparation comprising one or more members selected from compounds represented by formulae (1) and (2) and salts thereof is used as a collagen production inhibitor or a prophylactic or therapeutic agent for skin fibrotic diseases.

IPC Classes  ?

• A61K 31/405 - Indole-alkanecarboxylic acidsDerivatives thereof, e.g. tryptophan, indomethacin
• A61P 17/00 - Drugs for dermatological disorders
• A61P 17/06 - Antipsoriatics

Abstract

The present invention relates to a therapeutic substance delivery device for delivering a therapeutic substance to a desired site in a bodily duct, characterized in that the therapeutic substance delivery device is provided with: a therapeutic substance loading portion; a connector which is connected to the therapeutic substance loading portion; and a supplying/discharging pipe connected to the connector; and in that the therapeutic substance loading portion includes a main body portion in which a recessed portion is formed, a resilient film, and a connecting pipe; the connector is provided with a joint main body, a flange portion fixed to the other end portion side of the joint main body, and a fixing nut through which the joint main body passes; the joint main body is provided with a tube fastening portion; and at least part of the inner wall of the fixing nut is provided with a thread.

IPC Classes  ?

• A61B 17/00 - Surgical instruments, devices or methods

Abstract

The present invention pertains to a method for culturing a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells at a temperature of 40.5°C or higher and reducing the pluripotent stem cells included in the cell population. The present invention also pertains to a method for reducing pluripotent stem cells from a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells, wherein the method includes a step for activating the TRPV-1 expressed in the pluripotent stem cells included in the cell population. The presesnt invention makes it possible to reduce the pluripotent stem cells remaining in an undifferentiated state when inducing the differentiation of a pluripotent stem cell population.

IPC Classes  ?

• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 5/0735 - Embryonic stem cellsEmbryonic germ cells
• C12N 15/09 - Recombinant DNA-technology

Abstract

The present invention addresses the problem of obtaining an animal cell composition having a certain thickness by a simple and low-cost method. Specifically, the purpose of the present invention is to provide a method for eliminating the hypoxic state of an animal cell composition, and culturing an animal cell composition having a certain thickness, a method for producing an animal cell composition containing unicellular algae, and an animal cell composition. The present invention provides a method for culturing an animal cell composition in a liquid culture medium while irradiating the liquid culture medium with light in the presence of unicellular algae. According to the present invention, oxygen can be continuously supplied to the liquid culture medium, and a cell composition which has a certain thickness can be obtained without having a capillary network and in which damage to cells can be reduced.

IPC Classes  ?

• C12N 5/07 - Animal cells or tissues
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 1/12 - Unicellular algaeCulture media therefor

Abstract

A determination device is provided with a determination unit for determining the state of a cell using information on uniformity of a detection target generated on the basis of light intensity of light emitted from the detection target included in biological cells that have been irradiated with excitation light. In the determination device, the detection target may be a protein. In addition, the determination device may be provided with an information generating unit for generating information, wherein the information generating unit may generate information in which information corresponding to a non-resonant background signals is removed.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters

Abstract

Provided are layered cell sheets, comprising a plurality of layered cell sheets containing myoblasts, in which each cell sheet comprises cell population containing myoblasts with controlled orientations. Preferably provided are the layered cell sheets comprising a region in which the orientations of the cell population containing the myoblasts in each cell sheet are identical to each other.

IPC Classes  ?

• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• A61L 27/16 - Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds

Abstract

The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.

IPC Classes  ?

• A61F 2/18 - Internal ear or nose parts, e.g. ear-drums
• C12N 5/0793 - Neurons
• A61L 27/38 - Animal cells
• A61K 35/12 - Materials from mammalsCompositions comprising non-specified tissues or cellsCompositions comprising non-embryonic stem cellsGenetically modified cells

Abstract

The present invention provides a photosensitive composition which includes a copolymer containing constitutional units represented by formulae (1)-(3), a photoacid generator, and a solvent (the definition of the groups in the formulae is as described in the specification).

IPC Classes  ?

• G03F 7/038 - Macromolecular compounds which are rendered insoluble or differentially wettable
• C08F 220/56 - AcrylamideMethacrylamide
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus

Abstract

The present invention provides a cancer therapy method having reduced adverse side effects. Specifically, an anti-cancer agent comprising an anthracycline is used in such a manner that the anthracycline can be administered to a cancer patient at a dose of 0.5 to 7.5 mg/kg body weight in a combination therapy of a high intensity focused ultrasound therapy at a radiation intensity of 320 to 700 W/cm2 and an anti-cancer agent therapy.

IPC Classes  ?

• A61K 31/704 - Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin, digitoxin
• A61B 18/00 - Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
• A61K 9/51 - Nanocapsules
• A61P 35/00 - Antineoplastic agents

Abstract

The present invention enables statistical analysis of clinical data with a small number of samples. The following steps are included: a model generation step for performing iterative analysis of factor information items of patients which relate to the occurrence of adverse effects and which include an inspection value before dosing, and for modeling the trend of inspection values after dosing; and a distribution generation step for virtually generating, from the patient factor information items for which the inspection value trend has been modelled, patient factor-information items having the same factor information items as the patient factor-information items, and generates a frequency distribution for each of the factor information items for the patients for whom the fluctuation in the inspection values due to dosing is at or above a certain level, from among the patients having the generated factor information.

IPC Classes  ?

• G06Q 50/22 - Social work or social welfare, e.g. community support activities or counselling services
• G06Q 50/24 - Patient record management (processing of medical or biological data for scientific purposes G06F 19/00)

Abstract

Provided is an implant cultured periodontal ligament cell sheet complex that can be satisfactorily fixed to a bone with periodontal ligament tissue interposed therebetween. A dental implant fixture part is coated with calcium phosphate, and a cultured periodontal ligament cell sheet is brought into intimate contact with the implant.

IPC Classes  ?

• A61L 27/00 - Materials for prostheses or for coating prostheses
• A61K 35/32 - BonesOsteocytesOsteoblastsTendonsTenocytesTeethOdontoblastsCartilageChondrocytesSynovial membrane

Abstract

　The present invention provides a method for predicting the onset of glioblastoma in a test subject. The present invention is a method characterized by including: (1) a step for measuring the nestin concentration in a blood sample drawn from a test subject; and (2) a step for predicting a high likelihood that the test subject is suffering from glioblastoma, in the event that the nestin concentration is 0.63 ng/mL or above, and preferably 1.00 ng/mL or above.

IPC Classes  ?

• G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer

Abstract

Provided is a method for detecting the expression of SMN protein, said method comprises: a step for labeling SMN protein in a sample, said sample containing nucleated cells derived from blood; a step for labeling the nuclei of the nucleated cells in the sample; a step for selecting cell masses wherein the nuclei of the nucleated cells and the SMN protein are labeled; and a step for detecting the expression of the SMN protein on the basis of the label bound to the SMN protein in the cell masses that are selected above.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• G01N 21/64 - FluorescencePhosphorescence
• G01N 33/48 - Biological material, e.g. blood, urineHaemocytometers
• G01N 33/49 - Physical analysis of biological material of liquid biological material blood
• C07K 14/435 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans

Abstract

Disclosed herein is a cell culture apparatus that can achieve appropriate culture conditions. The cell culture apparatus (1) includes: a cylindrical culture vessel (10) that holds a culture liquid containing cells; a supporting column (20) that stands upright in a center of an inner surface of a bottom (12) in the culture vessel; and a stirring device (30) that includes an attaching portion (32) that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade (34) whose upper portion is fixed to the attaching portion so as to rotate around the supporting column.

IPC Classes  ?

• C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
• C12M 1/24 - Apparatus for enzymology or microbiology tube or bottle type
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• B01F 13/08 - Magnetic mixers
• B01F 15/00 - Accessories for mixers

Abstract

Provided is a cell culturing device capable of having a simple configuration and performing cell culturing well. The cell culturing device (5) comprises: a culture tank (15) housing a culture solution including cells; a shaft member (17) having at least part thereof arranged inside the culture tank (15); an agitation mechanism (19) supported by the shaft member (17), arranged inside the culture tank (15), and having at least a pair of agitation blades (27) rotatably provided that have the shaft member (17) as the rotation center thereof; and a filter (21) provided so as to come in contact with the shaft member (17) and attracting the culture solution from the culture tank (15) and/or supplying the culture solution to the culture tank (15). The shaft member (17) is at least partially hollow, has openings (17a, 17b) provided that guide the culture solution therein or guide the culture solution out from the interior thereof, cannot rotate, and draws the culture solution from the culture tank (15) and/or supplies the culture solution to the culture tank (15), via the filter (21) and the interior of the shaft member (17).

IPC Classes  ?

• C12M 3/02 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
• C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means

Abstract

A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means

Abstract

The present invention provides an artificial kidney precursor containing a non-human mammalian metanephros separated out from a living body, wherein the metanephros has been subjected to freezing and thawing treatments outside a living body, and contains mammalian mesenchymal stem cells transferred outside a living body, and a method of production thereof.

IPC Classes  ?

• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• A61K 35/22 - UrineUrinary tract, e.g. kidney or bladderIntraglomerular mesangial cellsRenal mesenchymal cellsAdrenal gland
• A61K 35/12 - Materials from mammalsCompositions comprising non-specified tissues or cellsCompositions comprising non-embryonic stem cellsGenetically modified cells

Abstract

Provided is a cell culture method. Cells are cultured using a medium that is substantially serum-free and contains (A) a retinoid and (B) an interleukin-1 inhibitor and/or a calpain inhibitor.

IPC Classes  ?

• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• C07K 14/715 - ReceptorsCell surface antigensCell surface determinants for cytokinesReceptorsCell surface antigensCell surface determinants for lymphokinesReceptorsCell surface antigensCell surface determinants for interferons
• C07K 14/81 - Protease inhibitors
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor

Abstract

Provided is a means for evaluating the wetting characteristic of an object such as a cell sheet and a culture dish in a non-contact fashion. The wetting characteristic of an object is evaluated by a method comprising the steps of: (1) removing a liquid by jetting a gas at a surface of the object covered with the liquid, (2) measuring a dimension of a region in which the liquid is removed after the completion of the gas jetting and (3) evaluating the wetting characteristic of the object using the measured dimension as an index.

IPC Classes  ?

• G01N 33/483 - Physical analysis of biological material
• G01N 13/00 - Investigating surface or boundary effects, e.g. wetting powerInvestigating diffusion effectsAnalysing materials by determining surface, boundary, or diffusion effects
• G01N 13/02 - Investigating surface tension of liquids

Abstract

An apparatus includes a brace being mounted on a person's body part and having a hard fitting surface. The apparatus also includes a load device, a support, a securing member, a brake, a switch device, and a soft film. The load device has a hard receiving surface receiving the fitting surface of the brace. The support movably supports the load device. The securing member secures the fitting surface to the receiving surface and enables the load device to move and follow movement of the person's body part and the brace against resistance applied from the support. The brake limits movement of the load device. The switch device switches operation modes of the apparatus, at least, between a free mode and a limiting mode. The soft film is arranged between the receiving and fitting surfaces for smooth movement of the person's body part on the load device in the limiting mode.

IPC Classes  ?

• A47F 5/00 - Show stands, hangers, or shelves characterised by their constructional features
• A61B 19/00 - Instruments, implements or accessories for surgery or diagnosis not covered by any of the groups A61B 1/00-A61B 18/00, e.g. for stereotaxis, sterile operation, luxation treatment, wound edge protectors(protective face masks A41D 13/11; surgeons' or patients' gowns or dresses A41D 13/12; devices for carrying-off, for treatment of, or for carrying-over, body liquids A61M 1/00)
• F16M 11/12 - Means for attachment of apparatusMeans allowing adjustment of the apparatus relatively to the stand allowing pivoting in more than one direction
• F16M 11/04 - Means for attachment of apparatusMeans allowing adjustment of the apparatus relatively to the stand
• F16M 11/06 - Means for attachment of apparatusMeans allowing adjustment of the apparatus relatively to the stand allowing pivoting
• F16M 11/24 - Undercarriages with or without wheels changeable in height or length of legs, also for transport only
• F16M 11/20 - Undercarriages with or without wheels

Abstract

This cell sheet laminate, which results from laminating a plurality of layers of cell sheets containing myoblasts, is characterized by each cell sheet containing a cell group containing myoblasts of which the orientation has been controlled. Preferably, the cell sheet laminate has a region at which the orientations of the cell groups containing myoblasts of each sheet are the same as each other.

IPC Classes  ?

• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12M 3/04 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing thin layers

Abstract

2 of the temperature-responsive polymer.

IPC Classes  ?

• B05D 1/00 - Processes for applying liquids or other fluent materials
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• C08F 293/00 - Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule

Abstract

The purpose of the present invention is to create an immune-tolerance inducer used in therapy in which donor hematopoietic cells are transplanted into a recipient in order to induce immune tolerance in the recipient with respect to donor cells, tissue, or organs. By using an alpha-galactosylceramide-containing liposome in combination with a costimulatory-pathway-blocking substance, hematopoietic chimerism can be induced in the recipient by the transplantation of donor hematopoietic cells, making it possible to induce immune tolerance in the recipient with respect to donor cells, tissue, or organs.

IPC Classes  ?

• A61K 31/7032 - Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a polyol, i.e. compounds having two or more free or esterified hydroxy groups, including the hydroxy group involved in the glycosidic linkage, e.g. monoglucosyl-diacylglycerides, lactobionic acid, gangliosides
• A61K 9/127 - Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
• A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
• A61K 45/00 - Medicinal preparations containing active ingredients not provided for in groups
• A61P 37/06 - Immunosuppressants, e.g. drugs for graft rejection
• A61P 43/00 - Drugs for specific purposes, not provided for in groups

Abstract

The purpose of the present invention is to precisely affix a sheet shaped therapeutic-use substance inside of a living organism. In the present invention, as shown in fig. 6(a), a sheet-shaped substance (40), in a state where wrinkles are formed therein, can be fit to a head (70). In such case, if the sheet-shaped substance (40) comes into contact with the outer peripheral portion of the head (70), the sheet-shaped substance (40) can be made to disperse, as shown in fig. 6(b), by making the pressure inside of a ventilation hole (71) positive. The surface of the head (70) has a protruding shape and therefore this surface is roughly parallel to the inner surface of an esophageal wall (300) to which the sheet-shaped substance (40) is to be affixed. As a result, such affixing (fig. 6(b)), in particular, can be carried out with greater precision.

IPC Classes  ?

• A61M 37/00 - Other apparatus for introducing media into the bodyPercutany, i.e. introducing medicines into the body by diffusion through the skin
• A61M 25/10 - Balloon catheters

Abstract

The invention relates to low-molecular-weight compounds which are capable of inducing differentiation of mesenchymal stem cell into hepatocytes.

IPC Classes  ?

• A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
• A61K 35/407 - LiverHepatocytes
• C07C 251/86 - Hydrazones having doubly-bound carbon atoms of hydrazone groups bound to carbon atoms of six-membered aromatic rings
• C07C 43/225 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing halogen
• C07D 487/04 - Ortho-condensed systems
• C07D 307/52 - Radicals substituted by nitrogen atoms not forming part of a nitro radical
• A61L 27/38 - Animal cells

Abstract

An automated culturing device, which uses culturing vessels having flat culturing surfaces, the automated culturing device being characterized: by having multiple levels of culturing vessel-holding shelves that hold multiple culturing vessels at intervals in the height direction, which is perpendicular to the culturing surface; by each of the culturing vessel-holding shelves holding the respective culturing vessels during culturing; and by being equipped with sensors, which are provided at each culturing vessel-holding shelf and are for detecting a posture or vibration of the shelf, and a drive system, which is capable of changing the posture of each culturing vessel-holding shelf between either a horizontal state or an inclined state in accordance with the sensor signal and maintains the horizontality of each culturing vessel-holding shelf during cell culturing.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12N 15/09 - Recombinant DNA-technology

Abstract

The present invention provides a method for producing a myocardial sheet using a group of cells derived from embryonic stem cells. This method is characterized by mixing Flk/KDR positive cells, cardiomyocytes, endothelial cells, and mural cells, all derived from embryonic stem cells, and culturing the mixed cells. Furthermore, the myocardial sheet can be used as a therapeutic agent for heart diseases since VEGF is released from the sheet.

IPC Classes  ?

• A61K 35/34 - MusclesSmooth muscle cellsHeartCardiac stem cellsMyoblastsMyocytesCardiomyocytes
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• A61K 35/44 - VesselsVascular smooth muscle cellsEndothelial cellsEndothelial progenitor cells
• A61K 9/70 - Web, sheet or filament bases

Abstract

Provided is a cell culture apparatus capable of actualizing a suitable culture environment. The cell culture apparatus (1) is provided with a cylindrical culture vessel (10) housing a culture medium containing cells; a support (20) standing upright from the center of the inner surface of the bottom (12) inside the culture vessel; and a stirring means (30) having an attaching portion (32) mounted, rotatably relative to the support, on the upper part of the support, and an impeller (34), the upper part of which is secured to the attaching portion, that rotates with the support as the rotation center.

IPC Classes  ?

• C12M 3/02 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
• C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 5/0735 - Embryonic stem cellsEmbryonic germ cells
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

Provided is a means of non-contact evaluation of the wettability of an object such as a cell sheet or culture dish. The wettability of the object is evaluated by means of a method containing: (1) a step in which a gas is emitted onto the surface of the object, which is covered by a liquid, thereby removing the liquid; (2) a step in which, after the gas has been emitted, a dimension is measured, said dimension being of the area from which the liquid was removed; and (3) a step in which the wettability of the object is evaluated using the measured dimension as an index.

IPC Classes  ?

• G01N 13/00 - Investigating surface or boundary effects, e.g. wetting powerInvestigating diffusion effectsAnalysing materials by determining surface, boundary, or diffusion effects

Abstract

The technology described herein relates to methods, assays, and compositions relating to causing a cell to assume a more pluripotent state, e.g. without introducing foreign genetic material.

IPC Classes  ?

• C12N 5/07 - Animal cells or tissues
• C12N 5/02 - Propagation of single cells or cells in suspensionMaintenance thereofCulture media therefor
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy

Abstract

A cell culture system equipped with a cell culture vessel, a composition-adjusted solution storage vessel, a culture solution composition adjustment means which has an inlet port and an outlet port for a cell culture solution, an inlet-connected liquid feeding circuit which is connected from the cell culture vessel to the inlet port of the culture solution composition adjustment means, an outlet-connected liquid feeding circuit which is connected from the cell culture vessel to the outlet port of the culture solution composition adjustment means, a means for perfusing the cell culture solution from the inlet-connected liquid feeding circuit to the outlet-connected liquid feeding circuit via the culture solution composition adjustment means, and a means for adjusting the amount of a liquid in the cell culture vessel, wherein both components for the cell culture solution in the cell culture vessel and components for the composition-adjusted solution in the composition-adjusted solution storage vessel can be controlled continuously through the culture solution composition adjustment means and the amount of the cell culture solution in the cell culture vessel can be adjusted substantially at a constant level.

IPC Classes  ?

• C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means

Abstract

A measuring unit which is to be attached to a cell culture container configured to accommodate cells to be cultured and a culture solution, includes: a measurer which is configured to measure information related to the cells and the culture solution, in a non-contact manner; and a sensor which is configured to detect at least one of a position, a posture, an impact, an orientation, and vibration.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Abstract

A method of measuring a liquid level of a cell culture solution stored in a cell vessel, includes: irradiating the cell culture solution with at least two kinds of light beams including a first light beam having a first wavelength and a second light beam having a second wavelength; measuring a first absorbance of the cell culture solution with respect to the first light beam, and a second absorbance of the cell culture solution with respect to the second light beam; and determining a liquid level of the cell culture solution based on the first absorbance and the second absorbance.

IPC Classes  ?

• G01F 23/00 - Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
• G01F 23/292 - Light

Abstract

A method for producing multilayered cell sheets, including producing a vascular bed which includes an artery-vein loop and in which a capillary vascular network is constructed; layering cell sheets on the vascular bed; and perfusing a culture medium in vitro to construct a vascular network in the cell sheets. The production method enables vascular networks to be constructed in cell sheets and enables thick multilayered cell sheets to foe easily produced by layering the cell sheets. Such thick multilayered cell sheets are useful as in vivo tissue-like products for regenerative medicine for various tissues and for evaluation of drugs and the like.

IPC Classes  ?

• A61K 35/44 - VesselsVascular smooth muscle cellsEndothelial cellsEndothelial progenitor cells
• A61K 35/34 - MusclesSmooth muscle cellsHeartCardiac stem cellsMyoblastsMyocytesCardiomyocytes
• A61K 35/36 - SkinHairNailsSebaceous glandsCerumenEpidermisEpithelial cellsKeratinocytesLangerhans cellsEctodermal cells
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
• A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof
• A61L 27/38 - Animal cells

Abstract

A method for manufacturing a multilayered cell sheet characterized in fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues.

IPC Classes  ?

• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• A61F 2/02 - Prostheses implantable into the body
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells

Abstract

As a result of using a cell culture substrate having a photopolymerization initiator fixed on the surface thereof and having linear polymers fixed on either part or all of the surface by means of the initiator, said photopolymerization initiator being thioxanthone, individual or multiple cells can be efficiently cultured on a specific region of the cell culture substrate, and can be efficiently detached by merely altering the temperature of the substrate surface.

IPC Classes  ?

• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus

Abstract

The purpose of the present invention is to provide a primer for amplifying a telomere sequence. The present invention provides a primer represented by formula (1) or formula (2), and for use in the amplification of a telomere sequence. (1) L1-(R1)n1. In the formula: the repeating units R1 represent a base sequence represented by GTAGGG; n1 represents 5 or 6; when n1 represents 5, L1 is a linker comprising 16 or fewer bases; and when n1 represents 6, L1 is a linker comprising 8 or fewer bases. (2) L2-(R2)n2(X). In the formula: the repeating units R2 represent a base sequence represented by GTAGGG; X represents a sequence obtained by removing or substituting one or two bases in the base sequence represented by GTAGGG; n2 represents 5 or 6; when n2 represents 5, L2 is a linker comprising 4-8 bases; and when n2 represents 6, L2 is a linker comprising 8 bases.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

The main purpose of the present invention is to provide a matrix for recovering cell fragments for therapeutic use whereby cell fragments capable of exerting a therapeutic effect at a high reproducibility can be obtained. The matrix for recovering cell fragments for therapeutic use, which comprises a stimulus-responsive area showing a change in cell adhesiveness due to a stimulus and a cell non-adhesive area having cell non-adhesiveness, characterized in that the circumference of the stimulus-responsive area is surrounded by the cell non-adhesive area. The above-said purpose can be achieved by providing this matrix.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12N 5/0775 - Mesenchymal stem cellsAdipose-tissue derived stem cells

Abstract

In order to provide a method for supporting surgery and a treatment support system with which it is possible to visualize remaining treatment regions in a high intensity focused ultrasound (HIFU) therapy, a treatment support system (10) is provided with: an ultrasonic probe (37) that performs a HIFU therapeutic treatment on a subject; an MRI device (1); an ultrasonic diagnostic therapy apparatus (40) that is positioned within the MRI device (1) and enables capturing ultrasonic images of the subject (24); and a three-dimensional position detecting device that detects the position of the ultrasonic probe (37). After treatment, remaining treatment regions are displayed by subtracting the plurality of images obtained by the MRI imaging before and after contrast radiography.

IPC Classes  ?

• A61B 5/055 - Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fieldsMeasuring using microwaves or radio waves involving electronic [EMR] or nuclear [NMR] magnetic resonance, e.g. magnetic resonance imaging
• A61B 8/00 - Diagnosis using ultrasonic, sonic or infrasonic waves
• A61B 17/22 - Implements for squeezing-off ulcers or the like on inner organs of the bodyImplements for scraping-out cavities of body organs, e.g. bonesSurgical instruments, devices or methods for invasive removal or destruction of calculus using mechanical vibrationsSurgical instruments, devices or methods for removing obstructions in blood vessels, not otherwise provided for
• A61B 18/00 - Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body

Abstract

The purpose is to select a low-molecular-weight compound which is effective for inducing the differentiation of a mesenchymal stem cell into a hepatocyte and develop a differentiation-inducing method which has excellent efficiency of inducing a mesenchymal stem cell into a hepatocyte and is safe. At least one compound selected from compounds represented by formula (1) and formula (2), a salt of the compound, or a solvate of the compound or the salt; a differentiation-inducing agent comprising at least one compound selected from compounds represented by formula (1) and formula (2), a salt of the compound, or a solvate of the compound or the salt; and a differentiation-inducing agent comprising a compound represented by formula (8), a salt of the compound or a solvate of the compound or the salt.

IPC Classes  ?

• C07C 251/86 - Hydrazones having doubly-bound carbon atoms of hydrazone groups bound to carbon atoms of six-membered aromatic rings
• A61L 27/00 - Materials for prostheses or for coating prostheses
• C07C 43/225 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing halogen
• C07D 307/52 - Radicals substituted by nitrogen atoms not forming part of a nitro radical
• C07D 487/04 - Ortho-condensed systems
• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues

Abstract

The present invention provides a method for producing a cardiomyocyte sheet using a group of cells derived from embryonic stem cells. The method is characterized by mixing Flk/KDR-positive cells, cardiomyocytes, endothelial cells and parietal cells all derived from embryonic stem cells and culturing the mixed cells. The cardiomyocyte sheet is so adapted as to release a VEGF therefrom, and therefore can be used as a therapeutic agent for heart diseases.

IPC Classes  ?

• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• A61K 35/12 - Materials from mammalsCompositions comprising non-specified tissues or cellsCompositions comprising non-embryonic stem cellsGenetically modified cells
• A61L 27/00 - Materials for prostheses or for coating prostheses
• A61P 9/00 - Drugs for disorders of the cardiovascular system
• A61P 43/00 - Drugs for specific purposes, not provided for in groups
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 5/0735 - Embryonic stem cellsEmbryonic germ cells
• C12N 15/09 - Recombinant DNA-technology

Abstract

A cell culture apparatus includes: an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed; a sampling unit configured to sample the culture solution in the cell incubator; a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.

IPC Classes  ?

• C12M 1/32 - Inoculator or sampler multiple field or continuous type
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

A pH measuring method includes: illuminating a medium solution, which includes: a first material; and a second material, with a plurality of light beams, which includes: a first light beam, a wavelength of which corresponds to a first absorption peak; a second light beam, a wavelength of which corresponds to a second absorption peak or a second convergence point; a third light beam, a wavelength of which corresponds to a third absorption peak or a third convergence point; and a fourth light beam, a wavelength of the fourth light beam at which an absorbance of at least one of the first and second materials is converged irrespective of a pH; receiving transmitted or reflected light beams of the first to fourth light beams; measuring absorbances at the wavelengths of the received light beams respectively; and calculating a pH of the medium solution based on the absorbances.

IPC Classes  ?

• G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
• G06F 19/00 - Digital computing or data processing equipment or methods, specially adapted for specific applications (specially adapted for specific functions G06F 17/00;data processing systems or methods specially adapted for administrative, commercial, financial, managerial, supervisory or forecasting purposes G06Q;healthcare informatics G16H)
• G01N 21/80 - Indicating pH value
• G01N 31/22 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroupsApparatus specially adapted for such methods using chemical indicators
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters

Abstract

An atom transfer radical polymerization initiator is immobilized on the surface of a monolithic porous body, and a polymer of which the hydration force can vary at temperatures ranging from 0 to 80˚C is grown from the initiator in the presence of a catalyst by an atom transfer radical method. In this manner, it becomes possible to produce a temperature-responsive monolithic porous body having, immobilized on the surface thereof at a high density, a polymer of which the hydration force can vary at temperatures ranging from 0 to 80˚C.

IPC Classes  ?

• G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups

Abstract

The production of an angiogenesis-promoting cytokine can be optimized and the formation of a vascular endothelial network can be optimized by altering the kinds of cells constituting a layered cell sheet, the mixing ratio thereof and the number of the disseminated cells to thereby change the conditions of the cells in the layered cell sheet.

IPC Classes  ?

• A61L 27/00 - Materials for prostheses or for coating prostheses
• C12M 3/04 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing thin layers

Abstract

A method of measuring a pH of a solution includes: emitting light beams of two wavelengths from one side of a measuring region of a solution into which an indicator is mixed, while pulsating the solution in the measuring region; receiving at least one of transmitted light beams and reflected light beams of the emitted light beams on the other side of the measuring region, while pulsating the solution in the measuring region; obtaining absorbances of the two wavelengths based on the received at least one of the transmitted light beams and the reflected light beams; obtaining an absorbance ratio from the obtained absorbances; and calculating a pH value of the solution based on the obtained absorbance ratio and an absorbance ratio/pH value correspondence database which is previously stored.

IPC Classes  ?

• G01N 21/80 - Indicating pH value
• G01N 31/22 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroupsApparatus specially adapted for such methods using chemical indicators
• G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters

Abstract

Provided is a cell culture treatment system for performing a culture treatment of cells or tissues in the fields of regenerative medicine and the like, whereby it becomes possible to keep the sterility of the system by preventing the invasion of viruses, human-derived cells other than those to be subjected to the culture treatment and the like from the outside of the system into the inside of closed spaces, and it also becomes possible to perform a cell culture treatment by combining multiple culture treatment modules by connecting or removing the multiple modules selectively in accordance with a wide variety of cell culture treatment processes while keeping the hermeticity of the closed spaces of the modules.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus

Abstract

The present invention provides a therapeutic agent or a preventative agent containing anti-VDAC antibody for diseases in which there is increased activation of osteoclasts, such as rheumatoid arthritis, and an inhibitor of human osteoclast formation containing anti-VDAC antibody.

IPC Classes  ?

• A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
• A61P 19/00 - Drugs for skeletal disorders
• A61P 19/08 - Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
• A61P 29/00 - Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agentsNon-steroidal antiinflammatory drugs [NSAID]
• A61P 35/04 - Antineoplastic agents specific for metastasis
• A61P 43/00 - Drugs for specific purposes, not provided for in groups

Abstract

[Problem] To provide a biomarker for the diagnosis of fatty liver diseases. [Solution] One or more compounds selected from the group consisting of etiocholanolone 3-sulfate, nervonic acid, α-tocopherol, 16-hydroxydehydroepiandrosterone 3-sulfate, dehydroepiandrosterone 2-sulfate, taurine, 15-hydroxyeicosatetraenoic acid, serotonin and thyroxine are used as biomarkers for the diagnosis, and fatty liver diseases are diagnosed by measuring the amounts of the compounds contained in a blood sample taken.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• G01N 27/62 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosolsInvestigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electric discharges, e.g. emission of cathode
• G01N 33/15 - Medicinal preparations
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
• G01N 33/74 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving hormones
• G01N 33/92 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving lipids, e.g. cholesterol
• A01K 67/027 - New or modified breeds of vertebrates