A method and system for generating a flow of droplets comprising providing a first droplet fluid, providing a second droplet fluid, providing a carrier fluid and forming a double emulsion of droplets each comprising a first droplet region comprising the first droplet fluid surrounded by a second droplet region comprising the second droplet fluid within the carrier fluid, by providing a flow of said first droplet fluid, a flow of said second droplet fluid, and a flow of said carrier fluid to a droplet generation sub-system of a microfluidic structure. The method comprises determining a dimension of said first droplet region of sequential droplets within the emulsion, determining an outer dimension of said second droplet region of sequential droplets within the emulsion, adjusting a pressure of the flow of the first droplet fluid in response to the determined dimension of the first droplet region, and adjusting a pressure of the flow of the second droplet fluid in response to the determined outer dimension of the second droplet region.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 15/0227 - Investigating particle size or size distribution by optical means using imagingInvestigating particle size or size distribution by optical means using holography
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
A method and system for determining droplet frequency of a flow of microfluidic droplets within a microfluidic droplet channel, comprising illuminating the flow of microfluidic droplets, using a beam splitter to split light from the flow of microfluidic droplets into a first portion and a second portion, directing the first portion to a camera, directing the second portion through an aperture located in front of a photodetector to the photodetector, and processing a signal from the photodetector and determining a droplet frequency from fluctuations in the processed signal. The method may be used to determine droplet dimension. The method may also be used to adjust a pressure of a flow of droplet fluid in response to a determined droplet dimension to generate a flow of droplets.
The present invention provides a surfactant comprising a poly(amidoamine) dendrimer and a perfluoropolyether, a polydimethylsiloxane or both, wherein said dendrimer comprises the unit (I).
ABSTRACT:
ABSTRACT:
An instrument for processing droplets in a microfluidic system. The instrument captures a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The instrument also processes each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. This involves processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The instrument controls a microfluidic process performed on the droplet, e.g. droplet dispensing, responsive to the estimated number of cells or other entities in the droplet. The instrument uses the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.
G06T 7/70 - Determining position or orientation of objects or cameras
G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersectionsConnectivity analysis, e.g. of connected components
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
A surfactant of formula (I):
A surfactant of formula (I):
A-(L1)a-(CH2)b-(L2)c-X (I)
wherein
A is a perfluoropolyether;
L1 is CONR′, wherein R′ is selected from H and C1-6 alkyl;
a is 0 or 1;
b is 0 or an integer between 1 and 10;
L2 is a linking group;
c is 0 or 1; and
X is a charged group.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals for use in industry and science; Chemical reagents, other than for medical or veterinary purposes; Chemical solutions, namely, gas soluble oils containing a surfactant, azo-dye, and biological reagents; Surfactants for use in drug discovery and development, medical diagnostic applications, genetic research, synthetic biology and life sciences research namely, fluorinated or nonfluorinated surfactants, biocompatible surfactants, emulsifiers, dispersants, wetting agents; Chemical coating agents for use in improving or enabling microfluidic processes namely, surfactants, polymers and other coating agents for enhancing fluid flow and controlling surface wettability in microfluidic devices and systems; Chemical agents for de-emulsifying solutions, namely, demulsifiers, destabilizers, and phase-separating compounds, proteins and enzymes for breaking down emulsions; unprocessed polymers; Pico droplets and microfluids for use in cell detection and molecular discovery, being microscale fluid compartments encapsulated in an immiscible carrier fluid or microscale fluid compartments surrounding by an immiscible fluid shell in an immiscible carrier fluid for use in cell detection and molecular discovery, other than for medical or veterinary purposes Laboratory apparatus and instruments, namely, pico droplet production, characterization, and selection instruments in the nature of microfluidic and emulsification instruments; Laboratory apparatus and instruments, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, droplet sorting modules, droplet dispensing modules, and integrated microfluidic components; Laboratory apparatus and instruments, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, droplet sorting modules, droplet dispensing modules, and integrated microfluidic components; Laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, pico droplet production, characterization, and selection instruments in the nature of microfluidic and emulsification instruments; Laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, droplet sorting modules, droplet dispensing modules, and integrated microfluidic components; Laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, droplet sorting modules, droplet dispensing modules, and integrated microfluidic components; Laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, pico droplet production, characterization, and selection instruments in the nature of microfluidic and emulsification instruments; Laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, droplet sorting modules, droplet dispensing modules, and integrated microfluidic components; Laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, droplet sorting modules, droplet dispensing modules, and integrated microfluidic components; Laboratory apparatus and instruments for the analysis of biomarkers, namely, pico droplet production, characterization, and selection instruments in the nature of microfluidic and emulsification instruments; laboratory apparatus and instruments for the analysis of biomarkers, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, droplet sorting modules, droplet dispensing modules, and integrated microfluidic components; Laboratory apparatus and instruments for the analysis of biomarkers, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, droplet sorting modules, droplet dispensing modules, and integrated microfluidic components; Replacement parts and fittings in the nature of tubing for scientific use, fluidic components namely, flow cells, controlled volume pumps and control valves for regulating the flow of gases and liquids, optical components namely, lasers for non-medical purposes, and light detectors, and electronic components namely, circuit boards, power supplies and electronic data processing apparatus for all the aforesaid goods Scientific research and development; Biopharmaceutical discovery, being drug discovery and biopharmaceutical drug development; Cell therapy engineering; Scientific research and development, namely, cell analysis and isolation for bioproduction, drug discovery, drug development, and synthetic biology
09 - Scientific and electric apparatus and instruments
Goods & Services
Laboratory apparatus and instruments; laboratory apparatus
and instruments for use in biopharmaceutical discovery and
development; laboratory apparatus and instruments for use in
cell detection and molecular discovery; laboratory apparatus
and instruments for the analysis, retrieval or discovery of
single cells; laboratory apparatus and instruments for use
in assuring monoclonality; parts and fittings for all the
aforesaid goods.
A method and system for determining droplet fre¬ quency of a flow of microfluidic droplets within a microfluidic droplet channel, comprising illuminating the flow of microfluidic droplets, using a beam splitter to split light from the flow of mi¬ crofluidic droplets into afirst portion and a second portion, directing the first portion to a camera, directing the second portion through an aperture located in front of a photodetector to the photodetec¬ tor, and processing a signal from the photodetector and determining a droplet frequency from fluctuations in the processed signal. The method may be used to determine droplet dimension. The method may also be used to adjust a pressure of a flow of droplet fluid in response to a determined droplet dimension to generate a flow of droplets.
A method and system for generating a flow of droplets comprising providing a first droplet fluid, providing a second droplet fluid, providing a carrier fluid and forming a double emulsion of droplets each comprising a first droplet region comprising the first droplet fluid surrounded by a second droplet region comprising the second droplet fluid within the carrier fluid, by providing a flow of said first droplet fluid, a flow of said second droplet fluid, and a flow of said carrier fluid to a droplet generation sub-system of a microfluidic structure. The method comprises determining a dimension of said first droplet region of sequential droplets within the emulsion, determining an outer dimension of said second droplet region of sequential droplets within the emulsion, adjusting a pressure of the flow of the first droplet fluid in response to the determined dimension of the first droplet region, and adjusting a pressure of the flow of the second droplet fluid in response to the determined outer dimension of the second droplet region.
A method and system for determining droplet frequency of a flow of microfluidic droplets within a microfluidic droplet channel, comprising illuminating the flow of microfluidic droplets, using a beam splitter to split light from the flow of microfluidic droplets into a first portion and a second portion, directing the first portion to a camera, directing the second portion through an aperture located in front of a photodetector to the photodetector, and processing a signal from the photodetector and determining a droplet frequency from fluctuations in the processed signal. The method may be used to determine droplet dimension. The method may also be used to adjust a pressure of a flow of droplet fluid in response to a determined droplet dimension to generate a flow of droplets.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemical reagents, other than for medical or veterinary
purposes; surfactants; biocompatible surfactants; chemical
reagents, containing an emulsion of non-ionic and
biocompatible surfactant and oil, for scientific purposes,
such as the analysis of single cells and molecules;
biocompatible surfactants for use in stabilising
pico-droplets used for single cell analysis.
09 - Scientific and electric apparatus and instruments
Goods & Services
laboratory apparatus and instruments, namely, laboratory apparatus and instruments for antibody discovery and assay isolation of cell lines expressing high levels of antibodies; laboratory apparatus and instruments for use in biopharmaceutical discovery and development, namely, for antibody discovery and assay isolation of cell lines expressing high levels of antibodies; laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, for antibody discovery and assay isolation of cell lines expressing high levels of antibodies; laboratory apparatus and instruments for the analysis, retrieval or discovery of single cells, namely, machines comprising or containing microfluidic pumps, biochips, optics and plate moving parts that aspirates, assays, sorts and dispenses single cells; laboratory apparatus and instruments for use in assuring monoclonality, namely, for single cell isolation and analysis; replacement parts for the foregoing laboratory apparatus and instruments
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals for use in industry and science; chemical
reagents; chemical solutions; surfactants; chemical coating
agents; chemical agents for de-emulsifying solutions;
polymers; picodroplets and microfluids for use in cell
detection and molecular discovery. Chemical preparations for medical purposes. Laboratory apparatus and instruments; laboratory apparatus
and instruments for use in cell detection and molecular
discovery; laboratory apparatus and instruments for the
analysis, retrieval or discovery of single cells, genes, or
populations of proteins and molecules, contained in
individual picodroplets; laboratory apparatus and
instruments for the analysis of biomarkers; parts and
fittings for all the aforesaid goods. Scientific research and development; biopharmaceutical
discovery and development; cell therapy engineering;
bioproduction and synthetic biology, analysis and isolation.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals for use in industry and science; chemical
reagents; chemical solutions; surfactants; chemical coating
agents; chemical agents for de-emulsifying solutions;
polymers; picodroplets and microfluids for use in cell
detection and molecular discovery. Chemical preparations for medical purposes. Laboratory apparatus and instruments; laboratory apparatus
and instruments for use in cell detection and molecular
discovery; laboratory apparatus and instruments for the
analysis, retrieval or discovery of single cells, genes, or
populations of proteins and molecules, contained in
individual picodroplets; laboratory apparatus and
instruments for the analysis of biomarkers; parts and
fittings for all the aforesaid goods. Scientific research and development; biopharmaceutical
discovery and development; cell therapy engineering;
bioproduction and synthetic biology, analysis and isolation.
15.
DROPLET SORTING BASED ON IONIZATION MASS SPECTROMETRY
A method for aligning sequences of droplets in streams of an emulsion comprising target droplets and tag droplets, a tag droplet comprising first and second tags. A target droplet is split into first and second target droplets and a tag droplet is split into first and second tag droplets. Each of the first and second tag droplets comprise the first and second tags. The first target droplet and first tag droplet are in a first stream of droplets, and the second target droplet and second tag droplet are in a second stream of droplets. The method detects the first tag droplets and first target droplets in the first stream and the second tag droplets and second target droplets in the second stream, determines a first sequence of droplets in the first stream and a second sequence of droplets in the second stream, and compares these to align the sequences.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals for use in industry and science; chemical reagents, other than for medical or veterinary purposes; chemical solutions, namely, gas soluble oils containing a surfactant, azo-dye, and biological reagents; surfactants for use in drug discovery and development, medical diagnostic applications, genetic research, synthetic biology and life sciences research namely, fluorinated or non-fluorinated surfactants, biocompatible surfactants, emulsifiers, dispersants, wetting agents; chemical coating agents for use in improving or enabling microfluidic processes namely, surfactants, polymers and other coating agents for enhancing fluid flow and controlling surface wettability in microfluidic devices and systems; chemical agents for de-emulsifying solutions, namely, demulsifiers, destabilizers, and phase-separating compounds, proteins and enzymes for breaking down emlusions; unprocessed polymers; pico droplets and microfluids for use in cell detection and molecular discovery being microscale fluid compartments encapsulated in an immiscible carrier fluid or microscale fluid compartments surrounding by an immiscible fluid shell in an immiscible carrier fluid for use in cell detection and molecular discovery, other than for medical or veterinary purposes Chemical preparations for medical purposes, namely, preparations for the treatment of cancer, cardiovascular, immunological, central nervous system, and metabolic diseases, genetic diseases, microbial infections, viral infections Laboratory apparatus and instruments, namely, pico droplet production instruments in the nature of microfluidic and emulsification instruments; Laboratory apparatus and instruments, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; Laboratory apparatus and instruments, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, pico droplet production instruments in the nature of microfluidic and emulsification instruments; laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, pico droplet production instruments in the nature of microfluidic and emulsification instruments; laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for the analysis of biomarkers, namely, pico droplet production instruments in the nature of microfluidic and emulsification instruments; laboratory apparatus and instruments for the analysis of biomarkers, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for the analysis of biomarkers, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; replacement parts and fittings in the nature of reflective mirrors for scientific use, tubing for scientific use, fluidic components namely, flow cells, controlled volume pumps and control valves for regulating the flow of gases and liquids, optical components namely, lasers for non-medical purposes, and light detectors, and electronic components namely, circuit boards, power supplies and electronic data processing apparatus for all the aforesaid goods Scientific research and development; biopharmaceutical discovery being drug discovery and biopharmaceutical drug development; cell therapy engineering; scientific research and development, namely, bioproduction and synthetic biology, analysis and isolation
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals for use in industry and science; chemical reagents, other than for medical or veterinary purposes; chemical solutions, namely, gas soluble oils containing a surfactant, azo-dye, and biological reagents; surfactants for use in drug discovery and development, medical diagnostic applications, genetic research, synthetic biology and life sciences research namely, fluorinated or non-fluorinated surfactants, biocompatible surfactants, emulsifiers, dispersants, wetting agents; chemical coating agents for use in improving or enabling microfluidic processes namely, surfactants, polymers and other coating agents for enhancing fluid flow and controlling surface wettability in microfluidic devices and systems; chemical agents for de-emulsifying solutions, namely, demulsifiers, destabilizers, and phase-separating compounds, proteins and enzymes for breaking down emlusions; unprocessed polymers; pico droplets and microfluids for use in cell detection and molecular discovery being microscale fluid compartments encapsulated in an immiscible carrier fluid or microscale fluid compartments surrounding by an immiscible fluid shell in an immiscible carrier fluid for use in cell detection and molecular discovery, other than for medical or veterinary purposes Chemical preparations for medical purposes, namely, preparations for the treatment of cancer, cardiovascular, immunological, central nervous system, and metabolic diseases, genetic diseases, microbial infections, viral infections Laboratory apparatus and instruments, namely, pico droplet production instruments in the nature of microfluidic and emulsification instruments; Laboratory apparatus and instruments, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; Laboratory apparatus and instruments, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, pico droplet production instruments in the nature of microfluidic and emulsification instruments; laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for use in cell detection and molecular discovery, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, pico droplet production instruments in the nature of microfluidic and emulsification instruments; laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for the analysis, retrieval and discovery of single cells, genes, or populations of proteins and molecules, contained in individual pico droplets, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for the analysis of biomarkers, namely, pico droplet production instruments in the nature of microfluidic and emulsification instruments; laboratory apparatus and instruments for the analysis of biomarkers, namely, single cell analysis and isolation systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; laboratory apparatus and instruments for the analysis of biomarkers, namely, cell engineering systems comprised of flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components; replacement parts and fittings in the nature of reflective mirrors for scientific use, tubing for scientific use, fluidic components namely, flow cells, controlled volume pumps and control valves for regulating the flow of gases and liquids, optical components namely, lasers for non-medical purposes, and light detectors, and electronic components namely, circuit boards, power supplies and electronic data processing apparatus for all the aforesaid goods Scientific research and development; biopharmaceutical discovery being drug discovery and biopharmaceutical drug development; cell therapy engineering; scientific research and development, namely, bioproduction and synthetic biology, analysis and isolation
We describe a microfluidic structure for spacing out droplets, the structure comprising: a main channel for guiding droplets in a spacing fluid; a first inlet for introducing droplets into the main channel; and a second inlet for introducing a spacing fluid into the main channel, wherein a cross-sectional area of the main channel decreases downstream from the first inlet. We also describe a method of spacing out droplets using a microfluidic structure.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemical reagents, other than for medical or veterinary purposes; surfactants for scientific use, namely for the analysis of cells and molecules; biocompatible surfactants for scientific use, namely for the analysis of cells and molecules; biocompatible surfactants for use in stabilising pico-droplets used for single cell analysis
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems in the nature of flow cytometers and flow-based analyzers providing cell and particle analysis, detection, and counting for scientific, laboratory, and general research uses, comprised of microfluidic devices being flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components for performing high-throughput analyses, separations, reactions, and measurements on cells, biomolecules, chemical compounds and biochemical analytes, microfluidic chips in the nature of microscope slides, miniaturized flow cells, micro-reactors, and lab-on-a-chip platforms for high-throughput analyses, separations, and reactions involving cells, biomolecules, and biochemical analytes, spectrophotometers for measuring nucleic acids, protein, organic molecules and small molecules, and detecting molecular interactions, mass spectrometers, and computer hardware and recorded software for running the aforementioned devices and providing chemical analysis on samples thereof; Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems for antibody discovery and assay isolation of cell lines expressing high levels of antibodies, for use in biopharmaceutical discovery and development, comprised of microfluidic pumps being syringe pumps, peristaltic pumps, diaphragm pumps, and piezoelectric pumps for precise fluid handling and control in miniaturized assay formats, all in the nature of pressure pumps for proportioning and testing purposes, biochips, optics and plate moving parts in the nature of microscopes and parts thereof that aspirates, assays, sorts and dispenses cells, and component parts and fittings for the foregoing laboratory apparatus and instruments
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems in the nature of flow cytometers and flow-based analyzers providing cell and particle analysis, detection, and counting for scientific, laboratory, and general research uses, comprised of microfluidic devices being flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components for performing high-throughput analyses, separations, reactions, and measurements on cells, biomolecules, chemical compounds and biochemical analytes, microfluidic chips in the nature of microscope slides, miniaturized flow cells, micro-reactors, and lab-on-a-chip platforms for high-throughput analyses, separations, and reactions involving cells, biomolecules, and biochemical analytes, spectrophotometers for measuring nucleic acids, protein, organic molecules and small molecules, and detecting molecular interactions, mass spectrometers, and computer hardware and recorded software for running the aforementioned devices and providing chemical analysis on samples thereof; Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems for antibody discovery and assay isolation of cell lines expressing high levels of antibodies, for use in biopharmaceutical discovery and development, comprised of microfluidic pumps being syringe pumps, peristaltic pumps, diaphragm pumps, and piezoelectric pumps for precise fluid handling and control in miniaturized assay formats, all in the nature of pressure pumps for proportioning and testing purposes, biochips, optics and plate moving parts in the nature of microscopes and parts thereof that aspirates, assays, sorts and dispenses cells, and component parts and fittings for the foregoing laboratory apparatus and instruments
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems in the nature of flow cytometers and flow-based analyzers providing cell and particle analysis, detection, and counting for scientific, laboratory, and general research uses, comprised of microfluidic devices being flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components for performing high-throughput analyses, separations, reactions, and measurements on cells, biomolecules, chemical compounds and biochemical analytes, microfluidic chips in the nature of microscope slides, miniaturized flow cells, micro-reactors, and lab-on-a-chip platforms for high-throughput analyses, separations, and reactions involving cells, biomolecules, and biochemical analytes, spectrophotometers for measuring nucleic acids, protein, organic molecules and small molecules, and detecting molecular interactions, mass spectrometers, and computer hardware and recorded software for running the aforementioned devices and providing chemical analysis on samples thereof; Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems for antibody discovery and assay isolation of cell lines expressing high levels of antibodies, for use in biopharmaceutical discovery and development, comprised of microfluidic pumps being syringe pumps, peristaltic pumps, diaphragm pumps, and piezoelectric pumps for precise fluid handling and control in miniaturized assay formats, all in the nature of pressure pumps for proportioning and testing purposes, biochips, optics and plate moving parts in the nature of microscopes and parts thereof that aspirates, assays, sorts and dispenses cells, and component parts and fittings for the foregoing laboratory apparatus and instruments
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems in the nature of flow cytometers and flow-based analyzers providing cell and particle analysis, detection, and counting for scientific, laboratory, and general research uses, comprised of microfluidic devices being flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components for performing high-throughput analyses, separations, reactions, and measurements on cells, biomolecules, chemical compounds and biochemical analytes, microfluidic chips in the nature of microscope slides, miniaturized flow cells, micro-reactors, and lab-on-a-chip platforms for high-throughput analyses, separations, and reactions involving cells, biomolecules, and biochemical analytes, spectrophotometers for measuring nucleic acids, protein, organic molecules and small molecules, and detecting molecular interactions, mass spectrometers, and computer hardware and recorded software for running the aforementioned devices and providing chemical analysis on samples thereof; Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems for antibody discovery and assay isolation of cell lines expressing high levels of antibodies, for use in biopharmaceutical discovery and development, comprised of microfluidic pumps being syringe pumps, peristaltic pumps, diaphragm pumps, and piezoelectric pumps for precise fluid handling and control in miniaturized assay formats, all in the nature of pressure pumps for proportioning and testing purposes, biochips, optics and plate moving parts in the nature of microscopes and parts thereof that aspirates, assays, sorts and dispenses cells, and component parts and fittings for the foregoing laboratory apparatus and instruments
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems in the nature of flow cytometers and flow-based analyzers providing cell and particle analysis, detection, and counting for scientific, laboratory, and general research uses, comprised of microfluidic devices being flow cells, droplet generators, segmented flow chips, single-cell encapsulation modules, and integrated microfluidic components for performing high-throughput analyses, separations, reactions, and measurements on cells, biomolecules, chemical compounds and biochemical analytes, microfluidic chips in the nature of microscope slides, miniaturized flow cells, micro-reactors, and lab-on-a-chip platforms for high-throughput analyses, separations, and reactions involving cells, biomolecules, and biochemical analytes, spectrophotometers for measuring nucleic acids, protein, organic molecules and small molecules, and detecting molecular interactions, mass spectrometers, and computer hardware and recorded software for running the aforementioned devices and providing chemical analysis on samples thereof; Scientific and laboratory apparatus, namely, microfluidic cell and molecular analysis systems for antibody discovery and assay isolation of cell lines expressing high levels of antibodies, for use in biopharmaceutical discovery and development, comprised of microfluidic pumps being syringe pumps, peristaltic pumps, diaphragm pumps, and piezoelectric pumps for precise fluid handling and control in miniaturized assay formats, all in the nature of pressure pumps for proportioning and testing purposes, biochips, optics and plate moving parts in the nature of microscopes and parts thereof that aspirates, assays, sorts and dispenses cells, and component parts and fittings for the foregoing laboratory apparatus and instruments
We describe a microfluidic structure for spacing out droplets, the structure comprising: a main channel for guiding droplets in a spacing fluid; a first inlet for introducing droplets into the main channel; and a second inlet for introducing a spacing fluid into the main channel, wherein a cross-sectional area of the main channel decreases downstream from the first inlet. We also describe a method of spacing out droplets using a microfluidic structure.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
We describe instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one embodiment a method of such processing comprises: providing a plurality of the entities in a fluid: preparing a droplet from said fluid: determining whether said droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of said determination; and dispensing said sorted droplet into a reservoir, wherein said dispensing comprises: identifying said sorted droplet for dispensing; extracting said sorted droplet from a first fluidic flow path of said fluid by transferring said sorted droplet from said first fluidic flow path into a second fluidic flow path; and ejecting said sorted droplet from said second fluidic flow path into said reservoir by applying pressure to said second fluidic flow path. In preferred embodiments the droplet contents are tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and afterwards retrieved for further processing or analysis, or for subsequent use, for example in fermenting or the like.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
C12M 1/00 - Apparatus for enzymology or microbiology
A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.
G06T 7/70 - Determining position or orientation of objects or cameras
G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersectionsConnectivity analysis, e.g. of connected components
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
A method of performing a biological reaction in a microfluidic droplet, and in particular to a method of performing genome editing in a microfluidic droplet. The invention also relates to microfluidic systems and instrumentations or products for performing these reactions.
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
c-X (I)
wherein
A is a perfluoropolyether;
1-6 alkyl;
a is 0 or 1;
b is 0 or an integer between 1 and 10;
2 is a linking group;
c is 0 or 1; and
X is a charged group.
This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities. We disclose herein a microfluidic droplet dispensing system comprising: a droplet feed line to carry droplets for dispensing; a dispensing line to carry dispensing fluid for dispensing the droplets; a droplet outlet line coupled to a droplet outlet; a first junction having a first input to receive a flow from said droplet feed line, having a second input to receive a flow from said dispensing line and having an output to said droplet outlet line; a waste line coupled to said droplet output line between said first junction and said droplet output; a first pressure source to provide pressurised dispensing fluid to said dispensing line to eject droplets from said droplet output; a droplet dispensing valve between said first pressure source and said dispensing line to control the ejection of droplets from said droplet output; wherein the system further comprises: a second pressure source to provide pressurised dispensing fluid to a droplet protect line; a second junction having a first input from said droplet feed line, having a second input from said droplet protect line, and having an output; and a volume flow throttle coupled between the output of said second junction and the first input of said first junction to restrict a volume flow from said second junction into said first junction.
A method for aligning sequences of droplets in streams of an emulsion comprising target droplets and tag droplets, a tag droplet comprising first and second tags. A target droplet is split into first and second target droplets and a tag droplet is split into first and second tag droplets. Each of the first and second tag droplets comprise the first and second tags. The first target droplet and first tag droplet are in a first stream of droplets, and the second target droplet and second tag droplet are in a second stream of droplets. The method detects the first tag droplets and first target droplets in the first stream and the second tag droplets and second target droplets in the second stream, determines a first sequence of droplets in the first stream and a second sequence of droplets in the second stream, and compares these to align the sequences.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
H01J 49/00 - Particle spectrometers or separator tubes
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
G06K 9/46 - Extraction of features or characteristics of the image
G06K 9/62 - Methods or arrangements for recognition using electronic means
a-A (VII), wherein A is a perfluoropolyether; a is a positive integer; X is either a covalent bond or a linking group; x is a positive integer; B is a polyalkylene oxide unit; n is a positive integer greater than 1 and, in compounds comprising more than one A, B, X, a and x, each may be the same or different. The present invention also relates to methods of making such surfactants, uses of such surfactants and emulsions comprising such surfactants.
C08G 65/332 - Polymers modified by chemical after-treatment with organic compounds containing oxygen containing carboxyl groups, or halides or esters thereof
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
C08G 65/333 - Polymers modified by chemical after-treatment with organic compounds containing nitrogen
C08G 65/334 - Polymers modified by chemical after-treatment with organic compounds containing sulfur
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C09K 23/00 - Use of substances as emulsifying, wetting, dispersing, or foam-producing agents
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Protein labelling kits comprising reagents and chemicals for
use in scientific and medical research; assay kits
consisting of chemical reagents for scientific and medical
research; assay kits consisting of biological reagents for
scientific and medical research; assay kits for labelling
proteins for scientific and medical research.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Protein labelling kits comprising reagents and chemicals for use in scientific and medical research; assay kits consisting of chemical reagents for scientific and medical research; assay kits consisting of biological reagents for scientific and medical research; assay kits for labelling proteins for scientific and medical research
Amethod of performing a biological reaction in a microfluidic droplet, and in particular to a method of performing genome editing in a microfluidic droplet. The invention also relates to microfluidic systems and instrumentations or products for performing these reactions.
B01F 17/00 - Use of substances as emulsifying, wetting, dispersing, or foam-producing agents
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
C08G 65/332 - Polymers modified by chemical after-treatment with organic compounds containing oxygen containing carboxyl groups, or halides or esters thereof
This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities. We disclose herein a microfluidic droplet dispensing system comprising: a droplet feed line to carry droplets for dispensing; a dispensing line to carry dispensing fluid for dispensing the droplets; a droplet outlet line coupled to a droplet outlet; a first junction having a first input to receive a flow from said droplet feed line, having a second input to receive a flow from said dispensing line and having an output to said droplet outlet line; a waste line coupled to said droplet output line between said first junction and said droplet output; a first pressure source to provide pressurised dispensing fluid to said dispensing line to eject droplets from said droplet output; a droplet dispensing valve between said first pressure source and said dispensing line to control the ejection of droplets from said droplet output; wherein the system further comprises: a second pressure source to provide pressurised dispensing fluid to a droplet protect line; a second junction having a first input from said droplet feed line, having a second input from said droplet protect line, and having an output; and a volume flow throttle coupled between the output of said second junction and the first input of said first junction to restrict a volume flow from said second junction into said first junction.
This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities. We disclose herein a microfluidic droplet dispensing system comprising: a droplet feed line to carry droplets for dispensing; a dispensing line to carry dispensing fluid for dispensing the droplets; a droplet outlet line coupled to a droplet outlet; a first junction having a first input to receive a flow from said droplet feed line, having a second input to receive a flow from said dispensing line and having an output to said droplet outlet line; a waste line coupled to said droplet output line between said first junction and said droplet output; a first pressure source to provide pressurised dispensing fluid to said dispensing line to eject droplets from said droplet output; a droplet dispensing valve between said first pressure source and said dispensing line to control the ejection of droplets from said droplet output; wherein the system further comprises: a second pressure source to provide pressurised dispensing fluid to a droplet protect line; a second junction having a first input from said droplet feed line, having a second input from said droplet protect line, and having an output; and a volume flow throttle coupled between the output of said second junction and the first input of said first junction to restrict a volume flow from said second junction into said first junction.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B81B 3/00 - Devices comprising flexible or deformable elements, e.g. comprising elastic tongues or membranes
B81B 7/02 - Microstructural systems containing distinct electrical or optical devices of particular relevance for their function, e.g. microelectro-mechanical systems [MEMS]
G01N 33/48 - Biological material, e.g. blood, urineHaemocytometers
A microfluidic structure for spacing out and aligning entities in an aqueous suspension is provided. The structure comprises: a channel for guiding entities in an aqueous suspension; a first comb of first inlets arranged on a first side of the channel for introducing a spacing medium into the channel; and a second comb of second inlets arranged on a second side of the channel for introducing the spacing medium into the channel; wherein the first side is opposite the second side, and wherein one of the first inlets has a corresponding, respective one of the second inlets at a substantially similar longitudinal position along the channel.
Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
C12M 1/00 - Apparatus for enzymology or microbiology
The present invention provides a surfactant having a formula selected from the group consisting of: B-((X)x-(CH2)a-A)n (VI), (A-(CH2)a-(X)x-B-(X)x-(CH2)a-A)n (IV), (A-(CH2)a- (Χ)x-Β)n (V), and (B)n-(X)x-(CH2)a-A (VII), wherein A is a perfluoropolyether; a is a positive integer; X is either a covalent bond or a linking group; x is a positive integer; B is a polyalkylene oxide unit; n is a positive integer greater than 1 and, in compounds comprising more than one A, B, X, a and x, each may be the same or different. The present invention also relates to methods of making such surfactants, uses of such surfactants and emulsions comprising such surfactants.
B01F 17/00 - Use of substances as emulsifying, wetting, dispersing, or foam-producing agents
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
C08G 65/332 - Polymers modified by chemical after-treatment with organic compounds containing oxygen containing carboxyl groups, or halides or esters thereof
50.
Droplet sorting based on ionization mass spectrometry
We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
H01J 49/00 - Particle spectrometers or separator tubes
We describe a microfluidic structure for spacing out and aligning entities in an aqueous suspension, the structure comprising: a channel for guiding entities in an aqueous suspension; a first comb of first inlets arranged on a first side of said channel for introducing a spacing medium into said channel; and a second comb of second inlets arranged on a second side of said channel for introducing said spacing medium into said channel; wherein said first side is opposite said second side, and wherein a said first inlet has a corresponding, respective one of said second inlets at a substantially similar longitudinal position along said channel.
We describe instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one embodiment a method of such processing comprises: providing a plurality of the entities in a fluid; preparing a droplet from said fluid; determining whether said droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of said determination; and dispensing said sorted droplet into a reservoir, wherein said dispensing comprises: identifying said sorted droplet for dispensing; extracting said sorted droplet from a first fluidic flow path of said fluid by transferring said sorted droplet from said first fluidic flow path into a second fluidic flow path; and ejecting said sorted droplet from said second fluidic flow path into said reservoir by applying pressure to said second fluidic flow path. In preferred embodiments the droplet contents are tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and afterwards retrieved for further processing or analysis, or for subsequent use, for example in fermenting or the like.
G01N 15/1492 - Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties within droplets
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
We describe instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one embodiment a method of such processing comprises: providing a plurality of the entities in a fluid; preparing a droplet from said fluid; determining whether said droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of said determination; and dispensing said sorted droplet into a reservoir, wherein said dispensing comprises: identifying said sorted droplet for dispensing; extracting said sorted droplet from a first fluidic flow path of said fluid by transferring said sorted droplet from said first fluidic flow path into a second fluidic flow path; and ejecting said sorted droplet from said second fluidic flow path into said reservoir by applying pressure to said second fluidic flow path. In preferred embodiments the droplet contents are tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and afterwards retrieved for further processing or analysis, or for subsequent use, for example in fermenting or the like.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemical reagents used for non-medical purposes; reagents, comprising an emulsion of non-ionic, biocompatible surfactant and oil, used for non-medical purposes such as single cell and molecule analysis; biocompatible surfactants for use in stabilising pico-droplets used for single cell analysis
A method of label-free cell or particle sorting in a microfluidic device includes providing a stream of aqueous droplets in oil in a channel of the microfluidic device, wherein at least some of the droplets include cells or particles and illuminating the stream from a first direction. Then, one detects scattered light from cells or particles within said aqueous droplets in a second direction. Next, one determines a number of the cells or particles in each droplet from the scattered light and sorts the aqueous droplets into differentiated streams. Notably, the refractive index of said oil is modified to closely match a refractive index of the aqueous droplets to reduce light scattered from boundaries of the droplets. A volume of the droplets can also controlled.
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.
09 - Scientific and electric apparatus and instruments
Goods & Services
Biochips for discovery and development of medical diagnostics and prognostics; bio-chips for research or scientific purposes; biochips for use in single cell analysis systems for therapeutic discovery, namely, instruments comprising microfluidic manifolds, pumps, electronics, optics, plate-moving automation and processing software; biochips for use with laboratory apparatus and instruments for use in biopharmaceutical discovery and development; biochips for use with laboratory apparatus and instruments for antibody discovery and assay isolation of cell lines
We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.
The present invention provides an emulsion for sorting droplets in a microfluidic device, the emulsion comprising: a discontinuous aqueous phase; and a continuous oil phase; wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.
We describe a method of label-free cell and/or particle sorting in a microfluidic device, the method comprising; providing a stream of aqueous droplets in oil in a channel of said microfluidic device, wherein at least some of said droplets include cells and/or particles illuminating said stream from a first direction; detecting, for example, scattered light from cells and/or particles within said aqueous droplets in a second direction; determining, for example, a number of said cells and/or particles in each droplet from the scattered light; and sorting said aqueous droplets into one of a plurality of differentiated streams responsive to said determined number. The method further comprises modifying a refractive index of said oil to more closely match a refractive index of said aqueous droplets to reduce light scattered from boundaries of said droplets. Optionally a volume of the droplets is also controlled. In embodiments the oil is fluorous oil. We describe examples of preferred refractive index modifying compounds.
09 - Scientific and electric apparatus and instruments
Goods & Services
Laboratory apparatus and instruments for use in biopharmaceutical discovery and development, namely, laboratory apparatus and instruments for antibody discovery and assay isolation of cell lines expressing high levels of antibodies; Laboratory apparatus and instruments for use in biopharmaceutical discovery and development, namely, machines comprising or containing microfluidic pumps, biochips, optics and plate moving parts that aspirates, assays, sorts and dispenses single cells; Component parts and fittings for the foregoing laboratory apparatus and instruments
A flow cell for a microfluidic device can include a chamber, first microfluidic entry and exit channels, second microfluidic entry and exit channels, a first electrode, and a second electrode. A microfluidic device can include a microfluidic channel, a laser to excite fluorescent material, and a detector to detect fluorescence emission. Methods of merging a droplet from an emulsion in to a second stream of fluid and of detecting a content of a droplet in a stream are further disclosed.
This invention relates to methods and systems for providing chemical and/or biological materials. Thus we describe a method of providing a chemical or biological material in a quantized form, the method comprising: preparing an emulsion comprising a plurality of microdroplets each holding a controlled amount of said material; storing said chemical or biological material in said microdroplets in said emulsion; transferring a portion of said emulsion microdroplets to a container for transportation; transporting said container to a user of said material, wherein said user receives said material in a quantized form in said microdroplets of said emulsion; and de-emulsifying said received microdroplets containing said material in quantized form to extract said material for subsequent use.
B65B 3/04 - Methods of, or means for, filling the material into the containers or receptacles
B65D 79/00 - Kinds or details of packages, not otherwise provided for
B65D 81/00 - Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
C12N 1/04 - Preserving or maintaining viable microorganisms
C12N 9/96 - Stabilising an enzyme by forming an adduct or a compositionForming enzyme conjugates
B65B 3/00 - Packaging plastic material, semiliquids, liquids or mixed solids and liquids, in individual containers or receptacles, e.g. bags, sacks, boxes, cartons, cans or jars
64.
METHOD OF PROVIDING A CHEMICAL OR BIOLOGICAL MATERIAL IN QUANTISED FORM AND SYSTEM THEREFOR
This invention relates to methods and systems for providing chemical and/or biological materials. Thus we describe a method of providing a chemical or biological material in a quantised form, the method comprising: preparing an emulsion comprising a plurality of microdroplets each holding a controlled amount of said material; storing said chemical or biological material in said microdroplets in said emulsion; transferring a portion of said emulsion microdroplets to a container for transportation; transporting said container to a user of said material, wherein said user receives said material in a quantised form in said microdroplets of said emulsion; and de-emulsifying said received microdroplets containing said material in quantised form to extract said material for subsequent use.
B65B 55/22 - Immersing contents in protective liquids
B65D 81/20 - Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
C12N 1/04 - Preserving or maintaining viable microorganisms
C12N 9/96 - Stabilising an enzyme by forming an adduct or a compositionForming enzyme conjugates
