Abstract

The devices and methods disclosed herein relate to combined microfluidic resistive pulse sensing and fluorescence to accurately and quantitatively measuring the size, concentration and single-particle fluorescence of nanoparticles in complex, heterogenous samples. The devices include an optics module, a cartridge interface, and a fluids module.

IPC Classes  ?

• G01N 15/02 - Investigating particle size or size distribution
• B01L 9/00 - Supporting devicesHolding devices
• G01N 15/06 - Investigating concentration of particle suspensions
• G01N 15/10 - Investigating individual particles
• G01N 15/12 - Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry

Abstract

Various embodiments herein disclose a device, comprising one or more fluid interfacing components and a cartridge holder, wherein the one or more fluid interfacing components are fixed while the cartridge holder moves along a linear guide. Also disclosed herein are methods of using the device to analyze a sample containing particles, and methods of diagnosing a disease in a subject by using the device.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

Various embodiments herein disclose a device, comprising one or more fluid interfacing components and a cartridge holder, wherein the one or more fluid interfacing components are fixed while the cartridge holder moves along a linear guide. Also disclosed herein are methods of using the device to analyze a sample containing particles, and methods of diagnosing a disease in a subject by using the device.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 9/00 - Supporting devicesHolding devices
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
• C12M 1/00 - Apparatus for enzymology or microbiology
• C40B 60/00 - Apparatus specially adapted for use in combinatorial chemistry or with libraries
• G01N 15/10 - Investigating individual particles
• G01N 15/12 - Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
• G01N 15/00 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials

Abstract

Various embodiments disclosed herein comprise a microfluidic cartridge, comprising a molded polymer bonded to a flat surface, wherein the molded polymer comprises one or more openings for connecting to fluidic volumes. Also provided are methods of preparing a microfluidic cartridge, comprising placing a patterned micro-fabricated chip into a mold and filling the mold with a material in liquid or other shape-conforming form. Further disclosed herein are methods of analyzing a particle sample by using the microfluidic sample.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 15/00 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials

Abstract

Various embodiments herein disclose a device, comprising one or more fluid interfacing components and a cartridge holder, wherein the one or more fluid interfacing components are fixed while the cartridge holder moves along a linear guide. Also disclosed herein are methods of using the device to analyze a sample containing particles, and methods of diagnosing a disease in a subject by using the device.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 9/00 - Supporting devicesHolding devices
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
• C12M 1/00 - Apparatus for enzymology or microbiology
• C40B 60/00 - Apparatus specially adapted for use in combinatorial chemistry or with libraries
• G01N 21/01 - Arrangements or apparatus for facilitating the optical investigation
• G01N 27/07 - Construction of measuring vesselsElectrodes therefor
• G01N 27/30 - Electrodes, e.g. test electrodesHalf-cells

Abstract

Various embodiments disclosed herein comprise a microfluidic cartridge, comprising a molded polymer bonded to a flat surface, wherein the molded polymer comprises one or more openings for connecting to fluidic volumes. Also provided are methods of preparing a microfluidic cartridge, comprising placing a patterned micro-fabricated chip into a mold and filling the mold with a material in liquid or other shape-conforming form. Further disclosed herein are methods of analyzing a particle sample by using the microfluidic sample.

IPC Classes  ?

• G01N 33/94 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving narcotics

Goods & Services

Imaging apparatus and instruments for use in the study of proteins and peptides in drug development; Laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; Scientific apparatus and instruments, namely, fluid handling device used for disposable bioprocessing applications and parts and fittings therefor; Scientific instruments, namely, electronic analyzers for measuring, testing and detecting contaminants and environmental pollutants; Scientific instruments, namely, electronic analyzers for testing consumer products for the presence of contaminants; Water testing instrumentation for monitoring and detecting contamination