A preparation method for a yeast protein-dietary fiber compound, comprising: pretreating a biomass raw material, and then sequentially carrying out steam explosion treatment, water washing treatment, delignification treatment, low-enzyme-amount enzymatic hydrolysis, fed-batch liquid fermentation and spray drying treatment to obtain a yeast protein-dietary fiber compound. Also provided are a product prepared by using the preparation method, the use of the product, and a feed comprising the product.
A23K 10/37 - Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hayAnimal feeding-stuffs from material of fungal origin, e.g. mushrooms from waste material
A23K 10/14 - Pretreatment of feeding-stuffs with enzymes
A23K 10/12 - Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
A23K 50/30 - Feeding-stuffs specially adapted for particular animals for swine
A23K 50/60 - Feeding-stuffs specially adapted for particular animals for weanlings
2.
Maize ZmTAS3j Gene and Its Use in Improving Tolerance to Lead for Plant
The present disclosure relates to the ZmTAS3j mediation lead stress tolerance in maize. The sequence of ZmTAS3j is identified by SEQ ID NO:1. The invention cloned the gene of ZmTAS3j from maize, and through genetic transformation of Arabidopsis thaliana and maize, it was verified that the ZmTAS3j mediated lead stress tolerance in maize.
The present invention relates to a Zea mays ZmTAS3j gene and a use thereof in improvement of plant lead tolerance. The nucleotide sequence of the Zea mays ZmTAS3j gene is as shown in SEQ ID NO. 1. According to the present invention, first, the ZmTAS3j gene is cloned from Zea mays, and by means of genetic transformation of Arabidopis thaliana and Zea mays, it is verified that the ZmTAS3j gene can improve the tolerance of a plant to lead stress.
China National Tobacco Corporation Sichuan Company (China)
Sichuan Provincial Tobacco Company Liangshan Prefecture Company (China)
Inventor
Li, Bing
Li, Jie
Feng, Changchun
Wang, Changquan
Huang, Rong
Chen, Yulan
Tao, Qi
Abstract
A preparation method of a rice straw biochar loaded with Bacillus cereus, comprising: (1) washing, drying, grinding and sieving rice straws; (2) performing anaerobic pyrolysis treatment on the rice straws treated in the step (1) at 300-700° C. to obtain a biochar; (3) treating the biochar obtained in the step (2) with hydrochloric acid, then washing until a washing solution is neutral, drying, grinding, sieving and sterilizing to obtain a sterilized biochar; and (4) performing mixed culture on the sterilized biochar and a solution of Bacillus cereus subjected to activation culture, and then centrifuging after the end of culture to remove supernatant, so as to obtain a rice straw biochar loaded with Bacillus cereus having a carbon immobilizing capability.
A cylindrical coordinate shearing type fruit picking end effector and a use method therefor are provided. The cylindrical coordinate shearing type fruit picking end effector includes a rack, a fruit clamping mechanism, a rotary motion mechanism, a rotary angle detection mechanism, a linear motion mechanism, a shearing motion mechanism, and a linear distance detection mechanism; where the linear distance detection mechanism is arranged at a front end of the linear motion mechanism; the shearing motion mechanism is arranged at a rear end of the shearing motion mechanism; the linear motion mechanism is arranged at a top end of the rotary motion mechanism; the rotary motion mechanism is arranged at a rear end of the rack; the rotary angle detection mechanism is arranged at a front end of the rotary motion mechanism; and the fruit clamping mechanism is arranged at a front end of the rack.
The present invention relates to a desert greenhouse framework system and a control method therefor. The desert greenhouse framework system comprises: a greenhouse framework comprising an overground framework and an underground framework which are detachably assembled; a plurality of freezers operably attached to the underground framework, configured in a plurality of groups in annular arrays, and at least partially placed in a sand layer; and a controller communicatively coupled to the freezers and configured to, on the basis of the temperature of the sand layer and the distribution positions of the freezers, dynamically adjust freezing parameters of the freezers, so as to change the morphology of the sand layer corresponding to each freezer. By dynamically regulating the cooling efficiency of the freezers according to the temperature change of the sand layer outside the greenhouse, the present invention ensures the stability of the solid-state sand layer serving as a greenhouse foundation part and, according to the distribution positions of the freezers, differentially controls the cooling efficiency thereof, thus saving electric power resources in deserts while reinforcing sand layer structures.
The present invention relates to a desert greenhouse control method and a system. The system comprises: a main unit, used for constructing a greenhouse space for growing crops; a ventilation unit used for exchanging air between the greenhouse space and the outside space; and a temperature maintaining unit used for adjusting the temperature and the temperature distribution in the greenhouse space. In the greenhouse system, ventilation and temperature maintaining working conditions acting on the greenhouse space are formed by means of at least ventilation parameters of the ventilation unit and temperature maintaining parameters of the temperature maintaining unit. In response to a plurality of trigger signals, the greenhouse system updates and adjusts the ventilation and temperature maintaining working conditions by changing the ventilation parameters and temperature maintaining parameters. According to the control method and the system of the present application, by means of structural configurations and control and adjustment, the problem of obviously uneven heat distribution in space and time of greenhouse systems in desert and Gobi areas is solved, and the positive support level of the greenhouse system for crop growth and the capacity thereof to resist against and adapt to changes in desert and Gobi environments are improved by means of adaptive control of the temperature maintaining and ventilation working conditions.
A method for preventing and treating porcine infectious diarrhea, wherein lysophosphatidic acid is used as a feed additive for preventing and treating porcine infectious diarrhea, and the porcine infectious diarrhea is caused by Escherichia coli. The feed additive is a premix prepared by means of mixing lysophosphatidic acid and a carrier, and the dosage form thereof is an oral powder or granule, and the dosage of the lysophosphatidic acid premix added to the feed is 0.1%-0.5% of the total weight of the feed.
C07D 233/58 - Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, attached to ring carbon atoms with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, attached to ring nitrogen atoms
C07D 233/56 - Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, attached to ring carbon atoms
C07D 233/60 - Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, attached to ring carbon atoms with hydrocarbon radicals, substituted by oxygen or sulfur atoms, attached to ring nitrogen atoms
The present disclosure provides use of lysophosphatidic acid (LPA) as a feed additive in controlling porcine infectious diarrhea, and belongs to the technical field of feed nutrition. The LPA is a type of lipid substance that is produced endogenously in vivo and can reduce a large secretion level of intestinal fluid in piglets caused by Escherichia coli infection via inhibiting cystic fibrosis transmembrane-conductance regulator (CFTR)-dependent iodine efflux. In this way, a susceptibility of weaned piglets to the Escherichia coli is prevented to effectively maintain the intestinal health of piglets. LPA is an endogenous substance in vivo, and it is first discovered that adding the LPA into a feed can control Escherichia coli-caused infectious diarrhea in the piglets.
Disclosed is a heat collecting and releasing system for a greenhouse, comprising: collecting and releasing channel, wherein the heat collecting and releasing channel is arranged on a wall and/or a roof of a greenhouse, and the heat collecting and releasing channel is located in the greenhouse, the wall and/or the roof; the heat collecting and releasing channel is used for circulating a first medium; the wall and the roof of the greenhouse are both made of transparent materials and internally provided with vacuum layers, and the vacuum layers are used for preventing the heat in the heat collecting and releasing channel from being dissipated to the outside of the greenhouse; a heat storage box, used for storing a second medium; a heat exchange device, used for exchanging heat between the first medium and the second medium; and a fan, used for providing power for circular flow of the first medium. Also disclosed is a heat collecting and releasing method. The method comprises: when sunlight is strong in daytime and heat collection is needed, storing heat in a second medium by means of heat exchange between a first medium and the second medium, and when heat release is needed, dissipating the heat into a greenhouse by means of heat exchange between the first medium and the second medium. The present invention improves the heat collection and release performance of the greenhouse.
The present embodiments of the invention disclose methods for implementing ZmARGOS9 gene in drought resistance and high yield of maize. ZmARGOS9 is overexpressed in maize in the present disclosure. The high expression of ZmARGOS9 gene improves the drought resistance and yield of maize. Accordingly, it enables enrichment of drought-resistant and high-yielding genes, contributing to the security of the seed industry.
A low-oxygen environment spiral tunnel fire combustion system, comprising a main tunnel structure, the main tunnel structure being a spiral structure; a positioning support system, the main tunnel structure being arranged on the positioning support system, and the positioning support system being used for controlling the height and the angle of the main tunnel structure; an oxygen content control system, the oxygen content control system being communicated with the inside of the main tunnel structure; a combustion system, the combustion system being arranged in the main tunnel structure; and a monitoring system, the monitoring system being arranged in the main tunnel structure, and the monitoring system being electrically connected to a computer acquisition system. The present invention can simulate and research the working conditions of tunnels having different line forms, different spiral radii and different spiral heights at different scales of fire, so as to summarize and derive experimental data under different working conditions, providing corresponding bases for crowd evacuation in fire disasters.
G01N 31/12 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroupsApparatus specially adapted for such methods using combustion
14.
ILLUMINATION APPARATUS AND METHOD FOR MOBILE ANIMAL AND PLANT BREEDING DEVICE
Throughout the growth cycle of a plant, the effects of light on plant growth include photosynthesis and signalization. A high-energy reaction and a low-energy reaction of a plant require both different light intensities and different light cycles. Light for a high-energy reaction needs to alternately flash in conjunction with photosynthesis and respiration, while a low-energy reaction needs to be allocated according to the growth cycles and output targets of different plant species. On this basis, the present invention relates to an illumination apparatus for a mobile animal and plant breeding device. The illumination apparatus includes a planting monitoring unit, an illumination unit and a cloud service end, wherein the illumination unit includes light sources in at least three directions, the light sources respectively being an always-on first light source arranged at the top of a plant and second light sources arranged on two sides of the plant; and the planting monitoring unit can confirm the growth density of the plant by means of the bare ground area and the area covered by the plant. Different light formulas are allocated to different parts of a plant, so as to achieve the aim of regulating and controlling the growth of the plant.
Disclosed is a traditional Chinese medicine sachet, comprising at least two of medicine bags I, II, III, IV, and V. The medicine bag I comprises Radix Bupleuri, Radix Angelicae Sinensis, Radix Paeoniae Alba, Radix Salviae miltiorrhizae, Poria cocos, Rhizoma Cyperi, Acorus calamus, Lignum Santali Albi, Citri Sarcodactylis Fructus, myrrh, and Radix glycyrrhizae. The medicine bag II comprises Cortex Albiziae, lily, Flos Caryophylli, Radix Astragali, Radix Angelicae, Folium Mori, Flos Chrysanthemi, Herba Menthae, Juncus effuses, Spica Prunellae, and Radix Glycyrrhizae. The medicine bag III comprises ginseng, Rhizoma Atractylodis macrocephalae, Poria cocos, Pericarpium Citri Reticulatae, Radix Angelicae Sinensis, Radix Paeoniae Alba, Radix Aucklandiae, Fructus Aurantii Immaturus, Endothelium corneum gigeriae galli, Rhizoma Atractylodis, Radix Angelicae, and Fructus Tsaoko. The medicine bag IV comprises Agastache rugosus, Rhizoma Atractylodis, Radix Angelicae, Fructus Tsaoko, Acorus calamus, folium Artemisiae argyi, borneol, Notopterygium root, raw rhubarb, Lignum Santali Albi, and Fructus Evodiae. The medicine bag V comprises Chinese yam, Fructus Corni, Poria cocos, Rhizoma Alismatis, Radix Cyathulae, Fructus Lycii, Cortex Eucommiae, Ramulus Mori, Herba Leonuri, folium Artemisiae argyi, and mulberries.
CHINA NATIONAL TOBACCO CORPORATION SICHUAN COMPANY (China)
SICHUAN TOBACCO CORPORATION DEYANG BRANCH (China)
SICHUAN AGRICULTURAL UNIVERSITY (China)
Inventor
Zeng, Shuhua
Yang, Hao
Xiang, Huan
Guo, Shiping
Yang, Xingyou
Yang, Weili
Liu, Xiaoli
Yang, Minfeng
Long, Tao
Liu, Yajie
Liu, Lei
Zhang, Tong
Zhang, Hongqi
Abstract
Disclosed in the present application are a method and system for constructing a moisture content prediction model for cigar tobacco in the air-curing stage. The method comprises the following steps: collecting a tobacco leaf image, and preprocessing the tobacco leaf image so as to obtain a preprocessed image; extracting initial tobacco leaf features on the basis of the preprocessed image, and analyzing preferred tobacco leaf features on the basis of the initial tobacco leaf features; dividing the initial tobacco leaf features according to a preset division method to obtain an initial feature training set and an initial feature test set, and dividing the preferred tobacco leaf features according to the preset division method to obtain a preferred feature training set and a preferred feature test set; constructing a plurality of initial feature prediction models on the basis of the initial feature training set, and constructing a plurality of preferred feature prediction models on the basis of the preferred feature training set; and, on the basis of the initial feature test set and the preferred feature test set, comparing the plurality of initial feature prediction models with the plurality of preferred feature prediction models, so as to obtain an optimal prediction model.
Provided is use of Dendrobium officinale DoObgC and a variable spliceosome thereof in promoting embryonic axis elongation. The serial number of the Dendrobium officinale DoObgC nucleotide is KT359612.1. Also provided is a variable spliceosome with a nucleotide sequence set forth in any of SEQ ID NOs. 1-5. In an Arabidopsis thaliana plant overexpressing Dendrobium officinale DoObgC or the variable spliceosome thereof, the length of the embryonic axis is significantly greater than that of a wild-type plant.
A water recirculation-type greenhouse comprises a greenhouse body (1), a water collecting device (2) provided in the greenhouse body (1), an air inlet (3) and an air outlet (4) provided on the water collecting device (2), and a condensation unit (5) and a reheating unit (6) provided in the water collecting device (2); said greenhouse further comprises an energy supply system (7). Air in the greenhouse body (1) is directed into the water collecting device (2) via the air inlet (3), flows through the condensation unit (5) and then into the reheating unit (6), and finally is discharged again into the greenhouse body (1) from the air outlet (4).
Disclosed provides a compound nutrient capable of repairing damaged intestinal tract of piglets and application thereof. The compound nutrient comprises the following components in weight percentage: organic acid 30-50%, amino acid 20-30%, enzyme preparation 8-15%, Bacillus subtilis 5-15%, mineral element 4-6%, glucose 5-20%. The enzyme preparation includes amylase, lipase and protease, and the weight ratio of amylase, lipase and protease is 1-2:1:1-2. The compound nutrient can repair the intestinal health of piglets with diarrhea, reduce the rate of diarrhea, and improve the growth performance.
A fusion protein, an amino acid sequence thereof, a coding nucleotide sequence thereof, a preparation method thereof and a use thereof are in the technical field of agricultural biotechnology. The fusion protein contains or consists of at least three, four, five, six, seven, or eight same and/or different PAMP (Pathogen-Associated Molecular Pattern) polypeptides. Optionally, there is at least one linker or no linker between two adjacent PAMP polypeptides. A plurality of PAMP polypeptides are assembled into the fusion protein having multiple immune epitopes. The fusion protein may induce defense immune responses of plants, weaken infestation ability of pathogenic microorganisms and substantially improve the disease resistance of plants. The method for preparing the fusion protein combines technologies of PTI (PAMP-Triggered Immunity) mechanism and gene engineering to obtain the fusion protein having multiple immune epitopes can be used in preparation of plant immune PAMP polypeptides.
Disclosed is a piglet feed based on bacteria enzyme synergistic fermentation process. The piglet feed is composed of basic components and bacteria enzyme synergistic fermentation feed. Basic components include soybean protein concentrate, whey powder, fish meal, sodium chloride, choline chloride, stone powder, calcium hydrogen phosphate, composite vitamins, composite trace elements, and composite amino acids. The bacterial enzyme synergistic fermentation feed includes a fermentation substrate, an enzyme preparation, and a bacterial strain. The bacterial enzyme synergistic fermentation feed can not only improve the production performance of piglets, but also improve the utilization rate of feed nutrients, especially the utilization rate of feed phosphorus, thereby reducing the excretion of phosphorus in feces.
A23K 10/30 - Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hayAnimal feeding-stuffs from material of fungal origin, e.g. mushrooms
A61K 31/714 - Cobalamins, e.g. cyanocobalamin, vitamin B12
A61K 31/197 - Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
A61K 31/4188 - 1,3-Diazoles condensed with heterocyclic ring systems, e.g. biotin, sorbinil
A61K 31/519 - PyrimidinesHydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
A61K 31/455 - Nicotinic acid, i.e. niacinDerivatives thereof, e.g. esters, amides
A61K 31/375 - Ascorbic acid, i.e. vitamin CSalts thereof
A23K 10/16 - Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
22.
Application of Compounds Inhibiting Synthesis of Very Long Chain Fatty Acids in Preventing and Treating Microbial Pathogens and Method Thereof
An application and a method of compounds inhibiting synthesis of very long chain fatty acids (VLCFAs) in preventing and controlling microbial pathogens are provided, which relate to the technical field of plant pathology and plant disease prevention and control. In particular, an application method of a compound for inhibiting the synthesis of VLCFAs in preventing and treating microbial pathogens is provided. Research results associated with the methods show that taking the synthesis of VLCFAs as the target, microbial pathogens can be inhibited by using compounds that inhibit the synthesis of VLCFAs. Therefore, the compounds inhibiting the synthesis of VLCFAs can be used in preventing and treating microbial pathogens diseases, which provides a new idea or strategy for the prevention and treatment of microbial pathogens diseases, and provides more choices for the types of drugs for the prevention and treatment of pathogenic diseases.
A01N 47/16 - Carbamic acid derivatives, i.e. containing the group —O—CO—NThio-analogues thereof the nitrogen atom being part of a heterocyclic ring
A01N 37/22 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N, e.g. carboxylic acid amides or imidesThio-analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
23.
Aegilops tauschii in stripe rust resistance breeding of triticeae plants
Aegilops tauschii and its use thereof in stripe rust resistance breeding of Triticeae plants. Said gene has a sequence as shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, SEQ ID NO. 7, SEQ ID NO. 9, or SEQ ID NO. 10.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
24.
TIBETAN PIG FECAL MICROBIOTA CAPSULE FOR RELIEVING DIARRHEA IN WEANED PIGLET, AND PREPARATION METHOD THEREFOR
The present application belongs to the technical field of veterinary medicine, and discloses a method for preparing a Tibetan pig fecal microbiota capsule for relieving diarrhea in a weaned piglet. The method comprises collecting fresh rectal fecal microbiota from a Tibetan pig and preparing a Tibetan pig fecal microbiota suspension; centrifuging and purifying the Tibetan pig fecal microbiota suspension, removing supernatant, and mixing the supernatant with a sodium alginate solution, whey protein isolate, sucrose, mannitol and glycerol evenly according to a ratio; preparing the mixed solution into Tibetan pig fecal microbiota gel particles, allowing the gel particles to stand, and then further packaging the gel particles in a methylcellulose capsule shell to obtain the Tibetan pig fecal microbiota capsule. The Tibetan pig fecal microbiota capsules prepared by the present method completely preserve the bacterial diversity and activity in the Tibetan pig fecal microbiota. Moreover, the added glycerol, among others, can increase the activity of the bacteria in the Tibetan pig fecal microbiota capsule, enhance the effect of administering of an intestinal flora preparation, and significantly decrease the mortality rate of weaned piglets due to diarrhea.
Provided is a fusion protein comprising or consisting of at least three, four, five, six, seven, or eight identical and/or different PAMP molecular polypeptides, optionally having at least one linker or no linker between two adjacent PAMP molecular polypeptides. The fusion protein can induce immune response of plant defense, reduce the infection ability of pathogenic microorganisms, and improve the disease resistance of plants. A preparation method for the fusion protein comprises: combining PTI immune mechanism and gene engineering technology, so as to obtain the fusion protein of multiple immune epitopes which does not exist in nature. The method solves the problem that preparing a plant immune PAMP molecule polypeptide is high in production cost and not able to be used in agricultural production in long term.
Disclosed herein are a duck hepatitis A virus type 3 (DHAV-3) mutant CH-P60-117C and a construction method thereof. The DHAV-3 mutant CH-P60-117C is constructed by mutating A at position 117 of 5′-UTR of the genome of the DHAV-3 virulent strain to C; mutating T at position 1142 to A to mutate tyrosine-164 of VP0 protein of the parent strain to asparagine; and mutating C at position 4334 to A so that leucine-71 of the viral protein 2C of the parent strain is mutated to isoleucine.
Provided are a mutant strain of type 3 duck hepatovirus CH-P60-117C strain and a construction method therefor. Specifically, the nucleotide at position 117 of the genome 5'UTR of type 3 virulent duck hepatovirus strain is mutated from A to C; the nucleotide at position 1142 is mutated from T to A, such that the amino acid at position 164 of the VP0 protein of the virus is mutated from tyrosine of the parent strain to asparagine; and the nucleotide at position 4334 is mutated from C to A, so that the amino acid at position 71 of the viral 2C protein is mutated from leucine of the parent strain to isoleucine. The CH-P60-117C strain has good immunogenicity and genetic stability, and is not pathogenic to ducklings.
Disclosed are a KASP labeled primer for detecting a high molecular weight glutenin subunit Dy10-m619SN of the wheat, and an application thereof. The KASP labeled primer comprises a forward primer F1, a forward primer F2, and a reverse primer R1; or comprises a forward primer F1, a forward primer F2, and a reverse primer R2, wherein sequences of F1, F2, R1, and R2 are as represented by SEQ ID NO. 1-SEQ ID NO. 4. The molecular labeled primer provided by the present invention can be used for identifying an SNP mutation site of a subunit Dy10 to be detected of the wheat, can determine the phenotype of the high molecular weight glutenin subunit Dy10-m619SN of the wheat according to the SNP mutation site or is used in combination with an SDS-PAGE technology to detect the phenotype of the subunit to achieve the purpose of assisting in identifying the wheat to be detected.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
The present invention relates to the technical field of phytopathology and plant disease control. Disclosed are an application of a compound capable of inhibiting synthesis of very-long-chain fatty acids in prevention and treatment of pathogenic bacteria, and a method. Disclosed in the present invention is the application of the compound capable of inhibiting synthesis of very-long-chain fatty acids in prevention and treatment of the pathogenic bacteria. According to the present invention, research results show that an inhibition effect on the pathogenic bacteria can be generated by using the compound capable of inhibiting synthesis of very-long-chain fatty acids by taking synthesis of the very-long-chain fatty acids as a target. Therefore, the compound capable of inhibiting synthesis of the very-long-chain fatty acids can be used in prevention and treatment of diseases of pathogenic bacteria, provides a novel idea or strategy for prevention and treatment of diseases of the pathogenic bacteria, and also provides more selectivity for the categories of drugs used for preventing and treating the diseases of the pathogenic bacteria.
A01N 37/26 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N, e.g. carboxylic acid amides or imidesThio-analogues thereof containing the group Thio-analogues thereof
A01N 47/12 - Carbamic acid derivatives, i.e. containing the group —O—CO—NThio-analogues thereof containing a —O—CO—N group, or a thio-analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
Provided are a duck plague virus gE and gI dual-gene traceless deletion strain DPV CHv-delta gE + delta gI and a construction method therefor. A duck plague virus gE gene and a duck plague virus gI gene are deleted by means of two times of homologous recombination on a bacterial artificial chromosome recombination duck plague virus rescue system platform by using a GS1783 escherichia coli strain and a pEPkan-S plasmid, a MiniF element is deleted by using an intracellular spontaneous homologous recombination method, and thus the construction of a duck plague virus dual-gene traceless deletion strain free from exogenous base and MiniF element residue is completed for the first time.
Disclosed are a duck plague virus gC gene-deletion strain DPV CHv-ΔgC with a MiniF element removed and a construction method therefor. By using a GS1783 E. coli strain and a pEPKan-S plasmid, a MiniF element is deleted from a bacterial artificial chromosome recombinant duck plague virus rescuing system platform by means of an intracellular spontaneous homologous recombination method, such that the present invention finishes the construction of a duck plague virus traceless deletion strain without MiniF element residues for the first time, solves the problem that the MiniF element remains in a duck plague virus genome when a duck plague virus gene is deleted, and provides sufficient technical support for accurately exploring the functions of the duck plague virus gene and constructing attenuated live vaccines.
C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
C12N 7/01 - Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
C12N 15/66 - General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligationUse of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Provided is a Yr4DS gene of Aegilops tauschii and use thereof in stripe rust resistance breeding of Triticeae plants. Said gene has a sequence as shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, SEQ ID NO. 7, SEQ ID NO. 9, or SEQ ID NO.10.
C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
33.
USE OF YR4DS GENE OF AEGILOPS TAUSCHII IN STRIPE RUST RESISTANCE BREEDING OF TRITICEAE PLANTS
Provided is a Yr4DS gene of Aegilops tauschii and use thereof in stripe rust resistance breeding of Triticeae plants. Said gene has a sequence as shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, SEQ ID NO. 7, SEQ ID NO. 9, or SEQ ID NO.10.
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
34.
USE OF PLANT CRUDE EXTRACTS OF PUERARIA PEDUNCULARIS FOR PREPARATION OF MOLLUSCICIDES
Provided is a use of plant crude extracts from Pueraria peduncularis for preparation of pesticides against pomacea canaliculata and oncomelania snails, the method of extracting crude extracts from Pueraria peduncularis comprising the steps of: (1) drying the roots of Pueraria peduncularis at 50°C and grinding the dried roots of Pueraria peduncularis into crude pieces of a size ranging from 1 mm-5 mm; (2) using ether petroleum and chloroform as extraction solvents to extract the crude pieces obtained in step (1) in a sequential order, the weight ratio of the crude pieces to the extraction solvents being 1:3, and subsequently using methanol, 1-butanol, ethanol or isopropyl alcohol, or a combination thereof, as an extraction solvent to carry out the extraction at 40-70°C for 12-24 hours three times; and concentrating and drying the extracted liquid to obtain a concentrate, namely crude extracts of Pueraria peduncularis.
A01N 25/02 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
Compositions and methods for improving the pest resistance of plants. Plants and plant parts identified, selected and/or produced using the compositions and the methods.
Disclosed is a veterinary drug composition for preventing and treating mycotoxin poisoning, and a formulation and preparation method thereof. The veterinary drug composition for preventing and treating mycotoxin poisoning is prepared from the following traditional Chinese medicinal materials with various raw materials being in parts by weight: 5-8 parts of Radix bupleuri by weight, 3-6 parts of Scutellaria baicalensis by weight, 4-7 parts of Astragalus membranaceus by weight, 5-8 parts of Rhizoma alismatis by weight, and 4-6 parts of Schisandra chinensis by weight. The veterinary drug composition preventing and treating mycotoxin poisoning can effectively improve or reverse pathological damage in animals caused by mycotoxin poisoning and can reduce the mortality rate of animals after mycotoxin poisoning, thereby effectively preventing and treating mycotoxin poisoning in animals.
Provided are an insecticidal crystal protein cry gene (Cry30Ga1) of Bacillus thuringiensis (Bt) strain HS18-1 and its encoded protein. The gene or the expression vector thereof is useful for preparing transgenic plant and for improving insect resistance of plant, and the protein is useful for preparing Bt insecticide.
Provided are an insecticidal crystal protein cry gene (Cry52Ba1) of Bacillus thuringiensis (Bt) strain BM59-2 and its encoded protein. The gene or the expression vector thereof is useful for preparing transgenic plant and for improving insect resistance of plant, and the protein is useful for preparing Bt insecticide.
Provided are an insecticidal crystal protein cry gene (Cry56Aa1) of Bacillus thuringiensis (Bt) strain YWC2-8 and its encoded protein. The gene or the expression vector there of is useful for preparing transgenic plant and for improving insect resistance of plant, and the protein is useful for preparing Bt insecticide.
A novel Bt protein Cry4Cb2 and a gene encoding the protein are disclosed by the present invention. Said protein is 1) a protein consisting of the amino acid sequence represented by SEQ ID NO.2, or 2) a protein derived from the protein of 1) by substitution, deletion and/or addition of one or several amino acids in the amino acid sequence represented by SEQ ID NO.2 and having equivalent activity.
Provided are an insecticidal crystal protein cry gene (Cry4Cb1) of Bacillus thuringiensis (Bt) strain HS18-1 and its encoded protein. The gene or the expression vector thereof is useful for preparing transgenic plants and for improving insect resistance of plants, and the protein is useful for preparing Bt insecticide.
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