The present disclosure generally relates to a method and a system for controlling the pretreatment and/or enzymatic hydrolysis of a lignocellulosic material. The method and system allow for savings with respect to energy consumption and costs.
The present disclosure relates to an improved process for enzymatic hydrolysis of lignocellulosic feedstock comprising steps of subjecting the lignocellulosic feedstock to impregnation to obtain impregnated slurry; adjusting the concentration and pH of the treated slurry to obtain a stream; adding additives to the stream to obtain additive-treated stream and subjecting the additive-treated stream to enzymatic hydrolysis to obtain enzyme-hydrolysed stream comprising fermentable sugars. The invention also discloses the step of co-fermenting the enzyme-hydrolyzed stream to obtain fermentation product. The improved process for enzymatic hydrolysis of the lignocellulosic feedstock of the present invention is characterized in that the addition of additives to the stream increases the efficiency of enzymatic hydrolysis at least by 7%
The present disclosure generally relates to a controlled method for pretreatment and/or enzymatic hydrolysis of a lignocellulosic material. The method comprises first pretreating the lignocellulosic material suboptimally, and then subjecting the material to mechanical refining.
The present disclosure generally relates to a method for treatment of a lignocellulosic biomass. The method comprises the steps of pretreating the lignocellulosic biomass material in a pretreatment arrangement to form a pretreated biomass slurry; optionally hydrolysing the pretreated biomass slurry in a hydrolysis unit; retrieving gaseous SO2 from the pretreatment arrangement to provide an SO2 gas stream; treating the SO2 gas stream with an alkaline solution to provide a sulfite/bisulfite solution; supplying at least a part of the sulfite/bisulfite solution to at least one of: a) the lignocellulosic biomass material prior to pretreating the lignocellulosic biomass material in the pretreatment arrangement; b) the lignocellulosic biomass material n the pretreatment arrangement; c) the pretreated biomass slurry discharged from the reactor vessel; d) the hydrolysate or fermentable sugars of the hydrolysate.
The present disclosure generally relates to a method for controlled pretreatment of lignocellulosic biomass. The method comprises the steps of: Pretreating (S10) a lignocellulosic biomass material in a pretreatment arrangement, the pretreating comprising impregnating (S10A) the lignocellulosic biomass with an SO2 feed in an impregnation vessel of the pretreatment arrangement; collecting (S20) a number of process parameters of the pretreatment, which process parameters include at least a feed parameter related to the total amount of lignocellulosic biomass input to the pretreatment arrangement, and a dry matter parameter related to the dry matter content of lignocellulosic biomass input to the pretreatment arrangement; and adjusting (S30) the SO2 feed in response to the process parameters.
The present invention relates to method for a microbial fermentation process involving fermentation of a fermentation media derived from biomass to a target fermentation product. The method comprising: providing a reference curve defined by a predetermined weight ratio of galactose to xylose as a function of acetic acid concentration present in the fermentation media; providing a first biomass-based stream and a second biomass-based stream different to the first biomass-based stream, comprising galactose and xylose; mixing the first and second biomass-based streams; providing a fermentation media by treating the mixed first and second biomass-based streams, or treating the first and second biomass-based streams separately prior to the mixing, wherein the mixing of the first and second biomass-based streams results in a weight ratio of galactose to xylose in the fermentation media closer to the reference curve compared to at least one of the first and second biomass-based streams; and fermenting the fermentation media by means of fermentation microorganisms being a yeast able to ferment xylose, such that the xylose and galactose are simultaneously fermented into the same target fermentation product.
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass. The pretreatment arrangement (100) comprises a reactor vessel (101) having an upstream inlet (102) for receiving biomass and a downstream outlet for discharging biomass (103). The pretreatment arrangement (104) further comprises a sluice vessel (104). The present disclosure also relates to a method (200) for pretreating lig- nocellulosic biomass.
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass comprising a reactor vessel (101) having an upstream inlet (102) for receiving biomass and a downstream outlet (103) for discharging biomass. The pretreatment arrangement (100) further comprises a gas valve (104) and gas flow control means (106).
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass comprising a reactor vessel (101) extending along a longitudinal center line (102) and having an upstream inlet (103) for receiving biomass and a downstream outlet (104) for discharging biomass. The pretreatment arrangement (100) further comprising a scraping device (108) configured to scrape the interior walls (111) of the reactor vessel (101) and prevent the formation and build-up of deposits.
The present disclosure generally relates to a method and a system for forming a storage stable hydrolysate from a lignocellulosic material and to a hydrolysate formed by such a method. It also relates to the use of the hydrolysate to reduce and/or control microbial contamination during storage and/or fermentation. Additionally, the present disclosure relates to a method and a system for reducing and/or controlling microbial contamination in a separate hydrolysis and fermentation (SHF) process.
The present invention relates to a method for reducing inhibitory substances of a lignocellulosic biomass-based material. The method comprises: pretreating a lignocellulosic biomass material in a pretreatment arrangement and subjecting the lignocellulosic biomass material to an elevated temperature and pressure in a reactor vessel; hydrolysing the pretreated biomass material in a hydrolysis unit; and adding at least one reducing agent selected from sulfur oxy anions and sulfhydryl reagents to the lignocellulosic biomass-based material downstream of the reactor vessel for reducing inhibitory substances. The concentration of the reducing agent is adapted based on the temperature of the material where it is added.
C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
C12P 7/40 - Preparation of oxygen-containing organic compounds containing a carboxyl group
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
C12M 1/00 - Apparatus for enzymology or microbiology
12.
METHOD AND SYSTEM OF INCREASING THE FERMENTABILITY OF MICROBIAL FERMENTATION
The present disclosure relates to a method of co-fermentation of sugars from lignocellulosic biomass to a fermentation product. The method comprises the steps of: - providing a first lignocellulosic biomass-based stream and a second lignocellulosic biomass-based stream different to the first lignocellulosic biomass-based stream, the first and second lignocellulosic biomass-based streams together comprising xylose, glucose and mannose; - mixing the first and second lignocellulosic biomass-based streams; - treating the mixed first and second lignocellulosic biomass-based streams, or treating the first and second lignocellulosic biomass-based streams separately prior to the step of mixing, to provide a fermentation media comprising xylose, glucose and mannose, wherein the mixing of the first and second lignocellulosic biomass-based streams results in a ratio of xylose to mannose in the fermentation media within a first target range and/or results in a ratio of mannose to glucose in the fermentation media within a second target range; - co-fermenting the fermentation media by means of fermentation microorganisms of yeast to a fermentation product.
The present disclosure generally relates to a method for control of a microbial fermentation process involving fermentation of sugars from lignocellulosic biomass to fermentation products. The method comprising the steps of: providing a fermentation media comprising at least xylose and glucose; fermenting the fermentation media by means of fermentation microorganisms of yeast; and intentionally increasing the concentration of ethanol present during the fermentation to an elevated ethanol concentration by the addition of ethanol or an ethanol-producing substance.
The present disclosure generally relates to a method and a system for controlling the pretreatment and/or enzymatic hydrolysis of a lignocellulosic material. The method and system allow for savings with respect to energy consumption and costs.
The present disclosure generally relates to a controlled method for pretreatment and/or enzymatic hydrolysis of a lignocellulosic material. The method comprises first pretreating the lignocellulosic material suboptimally, and then subjecting the material to mechanical refining.
The present disclosure generally relates to a controlled method for pretreatment and/or enzymatic hydrolysis of a lignocellulosic material. The method comprises first pretreating the lignocellulosic material suboptimally, and then subjecting the material to mechanical refining.
The present disclosure generally relates to a method and a system for controlling the pretreatment and/or enzymatic hydrolysis of a lignocellulosic material. The method and system allow for savings with respect to energy consumption and costs.
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Energy production; processing of fuel materials; fuel
treatment services; refining services; fuel refining;
extraction of elements contained in waste residues;
extraction of minerals contained in waste residues; gas
production services; incineration of gases; conversion of
lignocellulose to cellulose sugar and bioethanol; recycling
of chemicals; conversion of targeted substrates into
targeted chemical preparations by treatment with enzyme
systems. Scientific and technology services; industrial research;
industrial process research; installation of computer
software; chemistry consultation; technological consultancy;
design services; research and development services in the
field of engineering. Licensing of technology.
19.
ARRANGEMENT AND METHOD FOR TREATMENT OF LIGNOCELLULOSIC BIOMASS
The present disclosure generally relates to a method for treatment of a lignocellulosic biomass. The method comprises the steps of pretreating the lignocellulosic biomass material in a pretreatment arrangement to form a pretreated biomass slurry; optionally hydrolysing the pretreated biomass slurry in a hydrolysis unit; retrieving gaseous SO2 from the pretreatment arrangement to provide an SO2 gas stream; treating the SO2 gas stream with an alkaline solution to provide a sulfite/bisulfite solution; supplying at least a part of the sulfite/bisulfite solution to at least one of: a) the lignocellulosic biomass material prior to pretreating the lignocellulosic biomass material in the pretreatment arrangement; b) the lignocellulosic biomass material in the pretreatment arrangement; c) the pretreated biomass slurry discharged from the reactor vessel; d) the hydrolysate or fermentable sugars of the hydrolysate.
The present disclosure generally relates to a method for controlled pretreatment of lignocellulosic biomass. The method comprises the steps of: Pretreating (S10) a lignocellulosic biomass material in a pretreatment arrangement, the pretreating comprising impregnating (S10A) the lignocellulosic biomass with an SO2 feed in an impregnation vessel of the pretreatment arrangement; collecting (S20) a number of process parameters of the pretreatment, which process parameters include at least a feed parameter related to the total amount of lignocellulosic biomass input to the pretreatment arrangement, and a dry matter parameter related to the dry matter content of lignocellulosic biomass input to the pretreatment arrangement; and adjusting (S30) the SO2 feed in response to the process parameters.
The present disclosure generally relates to a method for treatment of a lignocellulosic biomass. The method comprises the steps of pretreating the lignocellulosic biomass material in a pretreatment arrangement to form a pretreated biomass slurry; optionally hydrolysing the pretreated biomass slurry in a hydrolysis unit; retrieving gaseous SO2 from the pretreatment arrangement to provide an SO2 gas stream; treating the SO2 gas stream with an alkaline solution to provide a sulfite/bisulfite solution; supplying at least a part of the sulfite/bisulfite solution to at least one of: a) the lignocellulosic biomass material prior to pretreating the lignocellulosic biomass material in the pretreatment arrangement; b) the lignocellulosic biomass material in the pretreatment arrangement; c) the pretreated biomass slurry discharged from the reactor vessel; d) the hydrolysate or fermentable sugars of the hydrolysate.
The present disclosure generally relates to a method for controlled pretreatment of lignocellulosic biomass. The method comprises the steps of: Pretreating (S10) a lignocellulosic biomass material in a pretreatment arrangement, the pretreating comprising impregnating (S10A) the lignocellulosic biomass with an SO2 feed in an impregnation vessel of the pretreatment arrangement; collecting (S20) a number of process parameters of the pretreatment, which process parameters include at least a feed parameter related to the total amount of lignocellulosic biomass input to the pretreatment arrangement, and a dry matter parameter related to the dry matter content of lignocellulosic biomass input to the pretreatment arrangement; and adjusting (S30) the SO2 feed in response to the process parameters.
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Energy production; processing of fuel materials; fuel treatment services; refining services, namely, biorefinery services; fuel refining; extraction of elements contained in waste residues in the nature of cellulosic sugar; gas production services; incineration of gases; conversion of lignocellulose to cellulose sugar and bioethanol; recycling of chemicals; conversion of targeted substrates into targeted chemical preparations by treatment with enzyme systems Scientific and technological services and research and design relating thereto, namely, scientific research; industrial analysis and research services, namely, analysis of industrial fluids; engineering services in the nature of design and development of processes for the production of bioethanol; engineering services in the nature of design and development of processes for the extraction of chemicals from cellulose; installation services relating to computer software being the installation of computer software for bioethanol production plants; technological consultancy relating to the development of technologies, namely, consulting in the field of new product design Technology licensing, namely, licensing of intellectual property in the field of technology, computer software license
24.
ARRANGEMENT AND METHOD FOR PRETREATMENT OF BIOMASS
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass comprising a reactor vessel (101) having an upstream inlet (102) for receiving biomass and a downstream outlet (103) for discharging biomass. The pretreatment arrangement (100) further comprises a gas valve (104) and gas flow control means (106).
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass. The pretreatment arrangement (100) comprises a reactor vessel (101) having an upstream inlet (102) for receiving biomass and a downstream outlet for discharging biomass (103). The pretreatment arrangement (104) further comprises a sluice vessel (104). The present disclosure also relates to a method (200) for pretreating lignocellulosic biomass.
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass comprising a reactor vessel (101) having an upstream inlet (102) for receiving biomass and a downstream outlet (103) for discharging biomass. The pretreatment arrangement (100) further comprises a gas valve (104) and gas flow control means (106).
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass comprising a reactor vessel (101) extending along a longitudinal center line (102) and having an upstream inlet (103) for receiving biomass and a downstream outlet (104) for discharging biomass. The pretreatment arrangement (100) further comprising a scraping device (108) configured to scrape the interior walls (111) of the reactor vessel (101) and prevent the formation and build-up of deposits.
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass comprising a reactor vessel (101) extending along a longitudinal center line (102) and having an upstream inlet (103) for receiving biomass and a downstream outlet (104) for discharging biomass. The pretreatment arrangement (100) further comprising a scraping device (108) configured to scrape the interior walls (111) of the reactor vessel (101) and prevent the formation and build-up of deposits.
The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass. The pretreatment arrangement (100) comprises a reactor vessel (101) having an upstream inlet (102) for receiving biomass and a downstream outlet for discharging biomass (103). The pretreatment arrangement (104) further comprises a sluice vessel (104). The present disclosure also relates to a method (200) for pretreating lignocellulosic biomass.
A method and system for control of a microbial fermentation process involving co-fermentation of sugars from lignocellulosic biomass to fermentation products by means of fermentation microorganisms is provided. A residual sugar indicator parameter RSI is measured, which parameter directly or indirectly indicates the concentration of residual sugars, during fermentation in the fermentation vessel (31). The amount of fermentation media added to the fermentation vessel is automatically adapted in a predetermined manner in response to the residual sugar indicator parameter, so as to achieve efficient co-fermentation of sugars to fermentation products.
The present disclosure generally relates to a method and a system for forming a storage stable hydrolysate from a lignocellulosic material and to a hydrolysate formed by such a method. It also relates to the use of the hydrolysate to reduce and/or control microbial contamination during storage and/or fermentation. Additionally, the present disclosure relates to a method and a system for reducing and/or controlling microbial contamination in a separate hydrolysis and fermentation (SHF) process.
The present disclosure generally relates to a method and a system for forming a storage stable hydrolysate from a lignocellulosic material and to a hydrolysate formed by such a method. It also relates to the use of the hydrolysate to reduce and/or control microbial contamination during storage and/or fermentation. Additionally, the present disclosure relates to a method and a system for reducing and/or controlling microbial contamination in a separate hydrolysis and fermentation (SHF) process.
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Scientific and technological services and research and
design relating thereto; industrial analysis and research
services; design and development of processes for the
production of bioethanol; design and development of
processes for the extraction of chemicals from cellulose;
installation services relating to computer software for
bioethanol production plants; technological consultancy
relating to the development of technologies. Technology licensing.
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Energy production; Processing of fuel materials; Fuel treatment services; Refining services; Fuel refining; Extraction of elements contained in waste residues; Extraction of minerals contained in waste residues; Gas production services; Incineration of gases; Recycling of chemicals; Conversion of targeted substrates into targeted chemical preparations by treatment with enzyme systems. Science and technology services; Industrial research; Industrial process research; Installation of computer software; Chemistry consultation; Technological consultancy; Design services; Research and development services in the field of engineering. Licensing of technology.
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
(1) Scientific and technological services and research and design relating thereto, namely, scientific and research consultation in the field of fuels, diesel fuel, biofuel, biodiesel fuel and bio-based chemicals; industrial analysis and research services, namely, industrial analysis of technology specification of computer software systems and computer hardware of others, scientific and research consultation in the field of fuels, diesel fuel, biofuel, biodiesel fuel, and bio-based chemicals; design and development of processes for the production of bioethanol; design and development of processes for the extraction of chemicals from cellulose; installation services relating to computer software, namely, computer software installation and maintenance for bioethanol production plants; technological consultancy relating to the development of technologies, namely, consulting in the field of design and testing of new products for others.
(2) Technology licencing, namely, licensing of intellectual property, computer software licensing.
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Scientific and technological services and research and design relating thereto, namely, scientific research; industrial analysis and research services, namely, analysis of industrial fluids; engineering services in the nature of design and development of processes for the production of bioethanol; engineering services in the nature of design and development of processes for the extraction of chemicals from cellulose; installation services relating to computer software being the installation of computer software for bioethanol production plants; technological consultancy relating to the development of technologies, namely, consulting in the field of new product design Technology licencing, namely, licencing of intellectual property in the field of technology, computer software licensing
A method and system for control of a microbial fermentation process involving co-fermentation of sugars from lignocellulosic biomass to fermentation products by means of fermentation microorganisms is provided. A residual sugar indicator parameter RSI is measured, which parameter directly or indirectly indicates the concentration of residual sugars, during fermentation in the fermentation vessel (31). The amount of fermentation media added to the fermentation vessel is automatically adapted in a predetermined manner in response to the residual sugar indicator parameter, so as to achieve efficient co-fermentation of sugars to fermentation products.
A method and system for control of a microbial fermentation process involving co-fermentation of sugars from lignocellulosic biomass to fermentation products by means of fermentation microorganisms is provided. A residual sugar indicator parameter RSI is measured, which parameter directly or indirectly indicates the concentration of residual sugars, during fermentation in the fermentation vessel (31). The amount of fermentation media added to the fermentation vessel is automatically adapted in a predetermined manner in response to the residual sugar indicator parameter, so as to achieve efficient co-fermentation of sugars to fermentation products.
The present invention relates to a method for pretreating a softwood starting material comprising the following steps: a) pretreating the softwood starting material in a pretreatment process to form a slurry; b) measuring the concentration of glucose and the concentration of mannose in the slurry formed in step a); c) calculating a ratio glucose : mannose from the concentrations measured in step b) d) comparing the ratio calculated in step c) to a reference value and if the ratio calculated in step c) differs from the reference value, controlling at least one process parameter of the pretreatment process in response to the difference.
The present invention relates to a method for pretreating a softwood starting material comprising the following steps: a) pretreating the softwood starting material in a pretreatment process to form a slurry; b) measuring the concentration of glucose and the concentration of mannose in the slurry formed in step a); c) calculating a ratio glucose : mannose from the concentrations measured in step b) d) comparing the ratio calculated in step c) to a reference value and if the ratio calculated in step c) differs from the reference value, controlling at least one process parameter of the pretreatment process in response to the difference.
There is provided a method of producing a hydrolysate containing sugar from a lingocellulosic biomass, comprising the steps of: a) pretreating the lingocellulosic biomass in a pretreatment process to form a slurry comprising cellulosic particles; b) saccharification of the slurry obtained in step a) in the presence of hydrolytic enzymes in an enzymatic hydrolysis process to obtain a hydrolysate containing sugar; c) analyzing cellulosic particles present in the slurry obtained in step a) and/or cellulosic particles present in the enzymatic hydrolysis process during step b) using an image analysis method to obtain a data set; and d) controlling at least one process parameter of the pretreatment process in step a) and/or enzymatic hydrolysis process in step b) in response to the data set obtained in step c). A corresponding system is also provided.
G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
C12Q 3/00 - Condition-responsive control processes
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
C12M 1/00 - Apparatus for enzymology or microbiology
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
16 - Paper, cardboard and goods made from these materials
17 - Rubber and plastic; packing and insulating materials
20 - Furniture and decorative products
22 - Rope, netting, tents, awnings, sails and sacks; padding and stuffing materials
27 - Floor and wall coverings
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Chemicals used in industry, science and photography, as well as in agriculture, horticulture and forestry; Tempering and soldering preparations; Adhesives used in industry; Additives (chemical- ) for fuels; Cellulose; Acetate of cellulose, unprocessed; Cellulose esters for industrial purposes; Cellulose derivates (chemicals); Ethyl alcohol; Unprocessed plastics. Napkins (diapers) for children and adults; Materials for dressings. Electrical and optical cables. Plastic materials for packaging (not included in other classes); Bags made of plastics for packaging. Plastics in extruded form for use in manufacture; Packing, stopping and insulating materials; Flexible tubes; Flexible pipes, not of metal. Plastic tubs. Sacks and bags (not included in other classes). Floor coverings. Scientific and technological services and research and design relating thereto; Industrial analysis and research services; Design and development of processes for the production of bioethanol; Design and development of processes for the extraction of chemicals from cellulose; Installation services relating to computer software for bioethanol production plants; Technical consultancy and development of technologies; Research and development of new products, for others, in the field of plastic materials. Licensing of technology.
2), in an impregnation chamber to obtain impregnated material; and heating the impregnated material to obtain pre-treated material, wherein the cellulosic material is compressed right before or when it is transferred to the impregnation chamber. A corresponding system is also provided.
There is provided a method of producing a hydrolysate containing sugar from a lingocellulosic biomass, comprising the steps of: a) pretreating the lingocellulosic biomass in a pretreatment process to form a slurry comprising cellulosic particles; b) saccharification of the slurry obtained in step a) in the presence of hydrolytic enzymes in an enzymatic hydrolysis process to obtain a hydrolysate containing sugar; c) analyzing cellulosic particles present in the slurry obtained in step a) and/or cellulosic particles present in the enzymatic hydrolysisprocess during step b) using an image analysis method to obtain a data set; and d) controlling at least one process parameter of the pretreatment process in step a) and/or enzymatic hydrolysis process in step b) in response to the data set obtained in step c). A corresponding system is also provided.
There is provided a method of producing a hydrolysate containing sugar from a lingocellulosic biomass, comprising the steps of: a) pretreating the lingocellulosic biomass in a pretreatment process to form a slurry comprising cellulosic particles; b) saccharification of the slurry obtained in step a) in the presence of hydrolytic enzymes in an enzymatic hydrolysis process to obtain a hydrolysate containing sugar; c) analyzing cellulosic particles present in the slurry obtained in step a) and/or cellulosic particles present in the enzymatic hydrolysisprocess during step b) using an image analysis method to obtain a data set; and d) controlling at least one process parameter of the pretreatment process in step a) and/or enzymatic hydrolysis process in step b) in response to the data set obtained in step c). A corresponding system is also provided.
2 and/or CaO to the slurry to increase its pH to at least 8, said addition being carried out at a slurry temperature of at least 60° C.; c) reducing the pH of the slurry to below 7; and optionally cooling the slurry from step b) to a temperature below 60° C.; and d) adding hydrolytic enzymes to the slurry from c) and allowing the slurry to hydrolyze wherein no washing of the slurry is performed prior to step d).
C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
C12P 19/16 - Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
C08B 1/00 - Preparatory treatment of cellulose for making derivatives thereof
47.
METHOD OF INCREASING THE PRODUCTION OF BIOGAS FROM A WASTE ACTIVATED SLUDGE
The invention relates to a method for biogas production by co-digestion of waste activated sludge and lignocellulosic biomass. In order to increase biogas yield, activated sludge is mixed with lignocellulosic biomass which has been pre-treated at 120-250°C and a pH of 0.5-3.0, such that a mixture having a temperature of 75-99°C and a pH of 0.5-3.0 is obtained, and the mixture is incubated before being anaerobically digested. The invention also relates to a system for performing the method, wherein a unit (1) for pre-treatment of lignocellulosic biomass comprises a pressure reactor (27) and a flashing chamber (28), and wherein a steam outlet (31) of the flashing chamber is connected to a steam inlet (32) of a mixer (9) for mixing sludge and pre-treated biomass.
A method is provided for improving enzymatic hydrolysis in saccharification of a lignocellulosic material. The method is comprising pretreating the lignocellulosic material to obtain a slurry of pretreated lignocellulosic material; adding at least one reducing agent to the slurry of pretreated lignocellulosic material or the liquid fraction thereof to decrease the enzymatic hydrolysis inhibitory properties of slurry of the pretreated lignocellulosic material or the liquid fraction thereof; and subjecting the slurry of pretreated lignocellulosic material or the liquid fraction thereof to enzymatic hydrolysis in the presence of the at least one reducing agent.
The present invention relates to a method of production of a target chemical from a cellulosic material, comprising the steps of: a) pretreating the cellulosic material under overpressure; b) releasing pressure to obtain pretreated cellulosic material and flash steam; c) hydrolyzing and fermenting the pretreated cellulosic material to obtain a liquid containing the target chemical; and d) distilling the liquid from step c) to obtain a first target chemical stream and a first stillage stream, wherein at least part of the flash steam from step b) is supplied as direct steam in the distillation of step d).
2), in an impregnation chamber to obtain impregnated material; and heating the impregnated material to obtain pre-treated material, wherein the cellulosic material is compressed right before or when it is transferred to the impregnation chamber. A corresponding system is also provided.
01 - Chemical and biological materials for industrial, scientific and agricultural use
04 - Industrial oils and greases; lubricants; fuels
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Chemicals used in industry, science and photography, as well as in agriculture, horticulture and forestry; Tempering and soldering preparations; Adhesives used in industry; Additives (chemical- ) for fuels; Cellulose; Acetate of cellulose, unprocessed; Cellulose esters for industrial purposes; Cellulose derivates (chemicals); Ethyl alcohol. Industrial oils and greases; Lubricants; Dust absorbing wetting and binding compositions; Fuels (including motor spirit) and illuminants; Additives, non-chemical, to motor-fuel; Alcohol [fuel]; Electrical energy; Bioethanol. Treatment of materials; Production of energy and fuel; Services relating to the treatment, refining and processing of fuel; Extraction of chemicals from cellulose; Extraction of bioethanol from cellulose; Processing and refining of natural energy sources and fuels for the production of energy, electricity, cold, heat, air and water; Gas production. Scientific and technological services and research and design relating thereto; Industrial analysis and research services; Design and development of processes for the production of bioethanol; Design and development of processes for the extraction of chemicals from cellulose; Installation services relating to computer software for bioethanol production plants; Technical consultancy and development of technologies. Technology licensing.
52.
In situ detoxification of fermentation inhibitors with reducing agents
The present invention provides a method for decreasing the fermentation inhibition in a fermentation of cellulose-derived material in a fermentor, wherein fermentation inhibitory properties of the material subjected to fermentation is decreased by an addition of at least one reducing agent to the fermentor. Further, there is provided a method of increasing the fermentability of a fermentation process comprising the steps of measuring the fermentability of the fermentation process and if the fermentability is below a reference value, then adding at least one reducing agent to the fermentation process.
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
The present invention provides a method for decreasing the fermentation inhibition in a process for producing a target chemical from a pretreated cellulosic material, the process comprising enzymatic hydrolysis of the pretreated cellulosic material and fermentation of hydrolysed material, wherein the fermentation inhibitory properties of the material subjected to fermentation is decreased by an addition of at least one reducing agent to the pretreated material or hydrolysed material. Moreover, the present invention provides the use of dithionite for decreasing the fermentation inhibitory properties of a material being subjected to simultaneous enzymatic hydrolysis and fermentation.
The present invention relates to a method of enzymatic hydrolysis of a lignocellulosic material, comprising the steps of: a) pretreating the lignocellulosic material to obtain a slurry having a pH of less than 6; b) adding NaOH, Ca(OH)2 and/or CaO to the slurry to increase its pH to at least 8, said addition being carried out at a slurry temperature of at least 60 °C; c) reducing the pH of the slurry to below 7; and optionally cooling the slurry from step b) to a temperature below 60 °C; and d) adding hydrolytic enzymes to the slurry from c) and allowing the slurry to hydrolyze wherein no washing of the slurry is performed prior to step d)
C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
C12P 19/16 - Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
55.
METHODS FOR IMPROVEMENT OF ENZYMATIC HYDROLYSIS OF LIGNOCELLULLOSIC MATERIAL
The present invention relates to a method of enzymatic hydrolysis of a lignocellulosic material, comprising the steps of: a) pretreating the lignocellulosic material to obtain a slurry having a pH of less than 6; b) adding NaOH, Ca(OH)2 and/or CaO to the slurry to increase its pH to at least 8, said addition being carried out at a slurry temperature of at least 60 °C; c) reducing the pH of the slurry to below 7; and optionally cooling the slurry from step b) to a temperature below 60 °C; and d) adding hydrolytic enzymes to the slurry from c) and allowing the slurry to hydrolyze wherein no washing of the slurry is performed prior to step d)
C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
C12P 19/16 - Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
C02F 3/34 - Biological treatment of water, waste water, or sewage characterised by the microorganisms used
C12N 1/22 - Processes using, or culture media containing, cellulose or hydrolysates thereof
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
C12P 1/00 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes
C12P 7/00 - Preparation of oxygen-containing organic compounds
The present invention relates to a method of producing a target chemical from a cellulosic starting material, comprising the steps of: a) pretreating the cellulosic starting material in a pretreatment process to form a slurry; b) analyzing the slurry from step a) with NIR spectroscopy to obtain at least one NIR spectrum characterizing the slurry from step a) (first data set); c) subjecting the slurry from step a) to separate or simultaneous saccharification and fermentation in a saccharification and fermentation process to form a fermentation broth comprising the target chemical; d) analyzing the slurry in a stage of step c) with NIR spectroscopy to obtain at least one NIR spectrum characterizing the slurry of step c) (second data set); and e) controlling at least one process parameter of said pretreatment process and/or said saccharification and fermentation process in response to the first and the second data set.
G01N 21/35 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/32 - Inoculator or sampler multiple field or continuous type
58.
ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL IN THE PRESENCE OF SULFITE, DITHIONITE AND/OR DITHIOTHREITOL
A method is provided for improving enzymatic hydrolysis in saccharification of a lignocellulosic material. The method is comprising pretreating the lignocellulosic material to obtain a slurry ofpretreated lignocellulosic material; adding at least one reducing agent to the slurry of pretreated lignocellulosic material or the liquid fraction thereof to decrease the enzymatic hydrolysis inhibitory properties of slurry of the pretreated lignocellulosic material or the liquid fraction thereof; and subjecting the slurry of pretreated lignocellulosic material or the liquid fraction thereof to enzymatic hydrolysis in the presence of the at least one reducing agent.
A method is provided for improving enzymatic hydrolysis in saccharification of a lignocellulosic material. The method is comprising pretreating the lignocellulosic material to obtain a slurry ofpretreated lignocellulosic material; adding at least one reducing agent to the slurry of pretreated lignocellulosic material or the liquid fraction thereof to decrease the enzymatic hydrolysis inhibitory properties of slurry of the pretreated lignocellulosic material or the liquid fraction thereof; and subjecting the slurry of pretreated lignocellulosic material or the liquid fraction thereof to enzymatic hydrolysis in the presence of the at least one reducing agent.
A method for preparing a target chemical derivable from cellulosic biomass, involving detoxification of spent hydrolysate is provided. The method comprises the steps of providing cellulosic biomass, subjecting the cellulosic biomass to aqueous pretreatment, aqueous hydrolysis, and fermentation under conditions in which at least a part of the fermentable sugars are fermented into a primary target chemical, separating the primary target chemical from the fermented hydrolysate to provide a spent hydrolysate comprising inhibitory substances and detoxifying the spent hydrolysate by decreasing the concentration of at least one of the inhibitory substances using a detoxification biocatalyst selected from the group consisting of wild type, mutant and recombinant filamentous fungi and recirculating at least a part of the detoxified spent hydrolysate, optionally after further purification.
The present invention provides a method for decreasing the fermentation inhibition in a fermentation of cellulose-derived material in a fermentor, wherein fermentation inhibitory properties of the material subjected to fermentation is decreased by an addition of at least one reducing agent to the fermentor. Further, there is provided a method of increasing the fermentability of a fermentation process comprising the steps of measuring the fermentability of the fermentation process and if the fermentability is below a reference value, then adding at least one reducing agent to the fermentation process.
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
A method of pre-treating a cellulosic material before hydrolysis is provided. The method comprises the steps of: impregnating the cellulosic material with a reactive water-soluble gas, such as sulphur dioxide (SO2) or carbon dioxide (CO2), in an impregnation chamber to obtain impregnated material; and heating the impregnated material to obtain pre-treated material, wherein the cellulosic material is compressed right before or when it is transferred to the impregnation chamber. A corresponding system is also provided.
The present invention provides a method for decreasing the fermentation inhibition in a fermentation of cellulose-derived material in a fermentor, wherein fermentation inhibitory properties of the material subjected to fermentation is decreased by an addition of at least one reducing agent to the fermentor. Further, there is provided a method of increasing the fermentability of a fermentation process comprising the steps of measuring the fermentability of the fermentation process and if the fermentability is below a reference value, then adding at least one reducing agent to the fermentation process.
C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
Disclosed is a method for decreasing the fermentation inhibition in a process for producing a target chemical from a pretreated cellulosic material, the process comprising enzymatic hydrolysis of the pretreated cellulosic material and fermentation of hydrolysed material, wherein the fermentation inhibitory properties of the material subjected to fermentation are decreased by addition of at least one reducing agent to the pretreated material or hydrolysed material. Moreover, disclosed is the use of dithionite for decreasing the fermentation inhibitory properties of a hydrolysate derived from enzymatic hydrolysis of a cellulosic material being subjected to fermentation.
C12P 1/00 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes
C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
The present invention provides a method for decreasing the fermentation inhibition in a process for producing a target chemical from a pretreated cellulosic material, the process comprising enzymatic hydrolysis of the pretreated cellulosic material and fermentation of hydrolysed material, wherein the fermentation inhibitory properties of the material subjected to fermentation is decreased by an addition of at least one reducing agent to the pretreated material or hydrolysed material. Moreover, the present invention provides the use of dithionite for decreasing the fermentation inhibitory properties of a material being subjected to simultaneous enzymatic hydrolysis and fermentation.
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
C12P 1/00 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes
C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
The present invention provides a method for decreasing the fermentation inhibition in a process for producing a target chemical from a pretreated cellulosic material, the process comprising enzymatic hydrolysis of the pretreated cellulosic material and fermentation of hydrolysed material, wherein the fermentation inhibitory properties of the material subjected to fermentation is decreased by an addition of at least one reducing agent to the pretreated material or hydrolysed material. Moreover, the present invention provides the use of dithionite for decreasing the fermentation inhibitory properties of a material being subjected to simultaneous enzymatic hydrolysis and fermentation.
C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
A method of pre-treating a cellulosic material before hydrolysis is provided. The method comprises the steps of: impregnating the cellulosic material with a reactive water-soluble gas, such as sulphur dioxide (SO2) or carbon dioxide (CO2), in an impregnation chamber to obtain impregnated material; and heating the impregnated material to obtain pre-treated material, wherein the cellulosic material is compressed right before or when it is transferred to the impregnation chamber. A corresponding system is also provided.
The present disclosure provides a method for pre-treating non-wood lignocellulosic material containing less than 5 % (w/w) starch or sugar in a process for production of ethanol from lignocellulose, comprising the steps of: adding organic acid or organic acid-producing bacteria to the lignocellulosic material; storing the lignocellulosic material in the presence of the organic acid for a period of at least two weeks in an atmosphere of less than 5 % oxygen to obtain organic acid-impregnated material; and heating the organic acid-impregnated material at a temperature of at least 160 °C to obtain pre-treated lignocellulosic material, wherein no, or substantially no, inorganic acid or base, including SO2, is added in the method.
The present disclosure provides a method for pre-treating non-wood lignocellulosic material containing less than 5 % (w/w) starch or sugar in a process for production of ethanol from lignocellulose, comprising the steps of: adding organic acid or organic acid-producing bacteria to the lignocellulosic material; storing the lignocellulosic material in the presence of the organic acid for a period of at least two weeks in an atmosphere of less than 5 % oxygen to obtain organic acid-impregnated material; and heating the organic acid-impregnated material at a temperature of at least 160 °C to obtain pre-treated lignocellulosic material, wherein no, or substantially no, inorganic acid or base, including SO2, is added in the method.
The present invention provides a method for providing a target chemical, comprising: providing a cellulose-derived slurry comprising fermentable saccharides in one or several containers;subjecting cellulose-derived slurry from said one or several containers to separation to provide a liquid fraction comprising part of said fermentable saccharides from said cellulose-derived slurry and a suspended solids fraction, which is returned to said container; transferring said liquid fraction to a fermentor; fermenting fermentable saccharides ofsaid liquid fraction in said fermentor to provide a fermented liquid comprising said target chemical; and returning said fermented liquid to said one or several containers.
The present invention relates to the use of a comparatively cheap material for the containment of at least a strongly acidic mineral acid solution highly diluted with water, by means of which saccharides are extracted from lignocellulose material by hydrolysis at a temperature of 175-240° C. and a pressure of 6-34 bar (0.6-14 MPa) as well as a time of 1-60 minutes and is characterized in that the material comprises, in addition to iron in an amount greater than any other individual substance and usually at least 50% by weight, at least chromium in an amount of 10.5-30% by weight, nickel in an amount of 2.5-29% by weight, and molybdenum in an amount of 0-7% by weight. The invention also relates to an apparatus.
A method for preparing a target chemical derivable from cellulosic biomass, involving detoxification of spent hydrolysateis provided. The method comprises the steps of providing cellulosic biomass, subjecting the cellulosic biomass to aqueous pretreatment, aqueous hydrolysis, and fermentation under conditions in which at least a part of the fermentable sugars are fermented into a primary target chemical, separating the primary target chemical from the fermented hydrolysate to provide a spent hydrolysate comprising inhibitory substances and detoxifying the spent hydrolysate by decreasing the concentration of at least one of the inhibitory substances using a detoxification biocatalyst selected from the group consisting of wild type, mutant and recombinant filamentous fungi and recirculating at least a part of the detoxified spent hydrolysate, optionally after further purification.
A method for preparing a target chemical derivable from cellulosic biomass, involving detoxification of spent hydrolysate is provided. The method comprises the steps of providing cellulosic biomass, subjecting the cellulosic biomass to aqueous pretreatment, aqueous hydrolysis, and fermentation under conditions in which at least a part of the fermentable sugars are fermented into a primary target chemical, separating the primary target chemical from the fermented hydrolysate to provide a spent hydrolysate comprising inhibitory substances and detoxifying the spent hydrolysate by decreasing the concentration of at least one of the inhibitory substances using a detoxification biocatalyst selected from the group consisting of wild type, mutant and recombinant filamentous fungi and recirculating at least a part of the detoxified spent hydrolysate, optionally after further purification.
The present invention relates to a methods, products and uses for inhibiting corrosion. A provided method comprises the steps of: a) subjecting cellulosic biomass to hydrolysis to obtain a hydrolysate liquid; b) optionally, subjecting the hydrolysate liquid from step a) to one or more of the steps of further hydrolysis, fermentation and/or distillation;and c) using the hydrolysate liquid from step a) or b) to inhibit corrosion.
C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
C23F 11/10 - Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids using organic inhibitors
75.
METHOD OF PRODUCTION OF ETHANOL FROM TWO DIFFERENT STARTING MATERIALS
The present invention provides a method of improving the ethanol yield in production of an ethanol-containing product from a lignocellulosic biomass and a sugar product containing fermentable sugars derived from a sugar-rich material. The method comprises treatment, involving hydrolysis, of said lignocellulosic biomass in one or more steps to obtain lignocellulose--derived treatment products including fermentable sugars; and fermentation, using a fermenting agent, of a mixture comprising at least part of said lignocellulose-derived treatment products and said fermentable sugars derived from said sugar-rich material to obtain the ethanol-containing product, wherein an amount of said sugar product is mixed with an amount of at least one of the fol-lowing: (i) lignocellulose-derived material in the treatment; (ii) lignocellulose-derived treatment products from the treatment; and (iii) lignocellulose-derived treatment products in the fermentation, such that said fermentable sugars derived from said sugar-rich material and said at least part of said lignocellulose-derived treatment products are present in the mixture, and said amounts are controlled such that the fermenting agent is subjected to stress by lignocellulose-derived treatment products to the extent that the ethanol yield is improved. Further, a corresponding method using a starch-rich starting material is provided as well as a corre-sponding use, composition and system.
The present invention provides a method of improving the ethanol yield in production of an ethanol-containing product from a lignocellulosic biomass and a sugar product containing fermentable sugars derived from a sugar-rich material. The method comprises treatment, involving hydrolysis, of said lignocellulosic biomass in one or more steps to obtain lignocellulose-derived treatment products including fermentable sugars; and fermentation, using a fermenting agent, of a mixture comprising at least part of said lignocellulose-derived treatment products and said fermentable sugars derived from said sugar-rich material to obtain the ethanol-containing product, wherein an amount of said sugar product is mixed with an amount of at least one of the following: (i) lignocellulose-derived material in the treatment; (ii) lignocellulose-derived treatment products from the treatment; and (iii) lignocellulose-derived treatment products in the fermentation, such that said fermentable sugars derived from said sugar-rich material and said at least part of said lignocellulose-derived treatment products are present in the mixture, and said amounts are controlled such that the fermenting agent is subjected to stress by lignocellulose-derived treatment products to the extent that the ethanol yield is improved. Further, a corresponding method using a starch-rich starting material is provided as well as a corresponding use, composition and system.
The present invention relates to the use of a comparatively cheap material for the containment of at least a strongly acidic mineral acid solution highly diluted with water, by means of which saccharides are extracted from lignocellulose material by hydrolysis at a temperature of 175-240°C and a pressure of 6-34 bar (0.6-14 MPa) as well as a time of 1-60 minutes and is characterized in that the material comprises, in addition to iron in an amount greater than any other individual substance and usually at least 50 % by weight, at least chromium in an amount of 10.5-30 % by weight, nickel in an amount of 2.5-29 % by weight, and molybdenum in an amount of 0-7 % by weight. The invention also relates to an apparatus.
The present invention relates to the use of a comparatively cheap material for the containment of at least a strongly acidic mineral acid solution highly diluted with water, by means of which saccharides are extracted from lignocellulose material by hydrolysis at a temperature of 175-240°C and a pressure of 6-34 bar (0.6-14 MPa) as well as a time of 1-60 minutes and is characterized in that the material comprises, in addition to iron in an amount greater than any other individual substance and usually at least 50 % by weight, at least chromium in an amount of 10.5-30 % by weight, nickel in an amount of 2.5-29 % by weight, and molybdenum in an amount of 0-7 % by weight. The invention also relates to an apparatus.
