The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy-to-use manner. Related apparatus, systems, techniques, articles, and compositions are also described.
2.
DELIVERY PLATFORM WITH INTEGRATED NON-VIRAL TRANSFECTION AND CELL PROCESSING
The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner. Related apparatus, systems, techniques, articles and compositions are also described.
The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner in an increased environmental temperature. A population of adherent cells is contacted with an aqueous solution including a payload and an alcohol at a concentration (v/v) greater than 2% in an environment having a temperature greater than 25°C. Also provided herein are non-viral methods for reprogramming blood cells to induced pluripotent stem cells (iPSCs). Related apparatus, systems, techniques, articles and compositions are also described.
An apparatus is provided. The apparatus can include a housing having a first end and a second end. The first end can include a sample inlet and a gas inlet. The second end can include a sample outlet and a gas outlet. The apparatus can also include a sample delivery passage extending within the housing and fluidically coupling the sample inlet to the sample outlet. The apparatus can also include a gas delivery passage extending within the housing and fluidically coupling the gas inlet to the gas outlet. Systems and methods including the apparatus are also provided herein.
A61M 5/30 - Syringes for injection by jet action, without needle, e.g. for use with replaceable ampoules or cartridges
B05B 1/02 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner. Related apparatus, systems, techniques, articles and compositions are also described.
The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to mammalian cells.
The invention relates to methods of engineering cells (e.g., dendritic cells (DCs)) for vaccinations (e.g., COVID-19) using ethanol-based transient cell membrane permeabilization. Related methods, compositions, apparatus, systems, and articles as described and/or illustrated herein.
A61K 35/15 - Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cellsMyeloid precursor cellsAntigen-presenting cells, e.g. dendritic cells
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C07K 14/165 - Coronaviridae, e.g. avian infectious bronchitis virus
8.
ENGINEERING OF IMMUNE CELLS FOR EX VIVO CELL THERAPY APPLICATIONS
The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells, e.g. T cells.
The current subject matter provides a cell engineering platform of a diagnostic and clinical use scale for vector -free and/or viral delivery of payload/cargo compounds and compositions into non-adherent cells, lire platform achieves delivery to a large number of cells quickly in a closed system. Related apparatus, systems, techniques, articles and compositions are also described.
Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.
An apparatus includes a catheter, and an electrode and methods of delivering molecules to eukaryotic cells using such an apparatus. The catheter defines a fluidic channel and has a distal opening. The electrode is within the fluidic channel and is spaced a distance from the distal opening of the catheter. The catheter is arranged to prevent direct contact between any electrode of the apparatus and tissue. Related apparatus, systems, techniques and articles are also described.
B05B 5/00 - Electrostatic spraying apparatusSpraying apparatus with means for charging the spray electricallyApparatus for spraying liquids or other fluent materials by other electric means
Systems, devices, and methods for electro spinning are provided. For example, a system includes a collector including load sensors attached thereto, the collector configured to receive an extruded polymer; and an electro spinning melt head assembly positioned above the collector and configured to extrude the polymer. The electro spinning melt head assembly and/or the collector is configured to move. The melt head assembly includes a syringe assembly and at least one heating element configured to supply heat to the syringe assembly. The syringe assembly includes: a syringe including a passage extending from a proximal end, the passage configured to receive the polymer, and a nozzle configured to allow polymer to pass therethrough.
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
C12M 3/02 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
A61K 31/00 - Medicinal preparations containing organic active ingredients
The current subject matter includes an adaptive electrospray device that creates consistent output when operating in atmosphere (e.g., not a vacuum). For example, the current subject matter includes an adaptive system that can monitor two current reference points (at the emitter and counter-electrode, respectively), determine a change in emitter current that will account for the parasitic losses, and adjust the emitter current accordingly. In addition, the current subject matter includes a high- throughput adaptive electrospray device having an array of emitters that rapidly switches the electrical potential of different emitters in an array on and off at a predetermined sequence that mitigates or eliminates interference from neighboring emitters. Related apparatus, systems, techniques and articles are also described.
B05B 1/14 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with multiple outlet openingsNozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with strainers in or outside the outlet opening
B05B 12/08 - Arrangements for controlling deliveryArrangements for controlling the spray area responsive to condition of liquid or other fluent material discharged, of ambient medium or of target
B05B 12/18 - Arrangements for controlling deliveryArrangements for controlling the spray area for controlling the spray area using fluids, e.g. gas streams
B05B 5/025 - Discharge apparatus, e.g. electrostatic spray guns
B05B 1/26 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with means for mechanically breaking-up or deflecting the jet after discharge, e.g. with fixed deflectorsBreaking-up the discharged liquid or other fluent material by impinging jets
16.
VECTOR-FREE DELIVERY OF GENE EDITING PROTEINS AND COMPOSITIONS TO CELLS AND TISSUES
The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, systems, techniques, compositions, and articles are also described.
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
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