The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner in an increased environmental temperature. A population of adherent cells is contacted with an aqueous solution including a payload and an alcohol at a concentration (v/v) greater than 2% in an environment having a temperature greater than 25°C. Also provided herein are non-viral methods for reprogramming blood cells to induced pluripotent stem cells (iPSCs). Related apparatus, systems, techniques, articles and compositions are also described.
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.
The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.
An apparatus is provided. The apparatus can include a housing having a first end and a second end. The first end can include a sample inlet and a gas inlet. The second end can include a sample outlet and a gas outlet. The apparatus can also include a sample delivery passage extending within the housing and fluidically coupling the sample inlet to the sample outlet. The apparatus can also include a gas delivery passage extending within the housing and fluidically coupling the gas inlet to the gas outlet. Systems and methods including the apparatus are also provided herein.
The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to mammalian cells.
The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner. Related apparatus, systems, techniques, articles and compositions are also described.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Stem cells for research purposes Pharmaceutical preparations for the treatment of immune-oncological disorders, cancer, diabetes, respiratory, heart, neurological and auto immune disorders; stem cells for medical purposes; living cells for medical use Scientific apparatus and instruments, namely, scientific apparatus and instruments for the transfer of biological molecules into living cells; measuring apparatus and instruments, namely, pressure measuring apparatus; probes for scientific purposes, namely, ultrasound probes, not for medical use; bioassay apparatus, namely, pressure measuring apparatus for use in bioassay techniques other than medical; replacement parts and fittings for the aforesaid goods Surgical apparatus and instruments; medical apparatus and instruments for delivery of macro molecules to the mouth, nose, airway, lungs; veterinary apparatus and instruments, namely, catheter for veterinary purposes; medical and surgical probes; probes for medical use and medical diagnostic purposes; medical heartbeat measuring apparatus for use in bioassay techniques; replacement parts and fittings for the aforesaid goods; measuring apparatus and instruments, namely, heartbeat measuring apparatus Scientific and technological services, namely, research and design in the field of pharmaceutics, tissue engineering and regenerative medicine; analysis of industrial fluids and scientific research services; medical and scientific research; pharmaceutical research and development, and clinical testing, namely, conducting clinical trials for others in the field of pharmaceutical preparations, drugs, cell therapy, gene therapy and cancer therapy; development of probes, reagents and bioassays for industrial and scientific purposes; advisory, consultancy and information services relating to the provision of the aforesaid
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Stem cells for research purposes. Pharmaceutical preparations for the treatment of immune-oncological disorders, cancer, diabetes, respiratory, heart, neurological and auto immune disorders; stem cells for medical purposes; living cells for medical use. Scientific apparatus and instruments, namely, scientific apparatus and instruments for the transfer of biological molecules into living cells; measuring apparatus and instruments, namely, pressure measuring apparatus; probes for scientific purposes, namely, ultrasound probes, not for medical use; bioassay apparatus, namely, pressure measuring apparatus for use in bioassay techniques other than medical; replacement parts and fittings for the aforesaid goods. Surgical apparatus and instruments; medical apparatus and instruments for delivery of macro molecules to the mouth, nose, airway, lungs; veterinary apparatus and instruments, namely, catheter for veterinary purposes; medical and surgical probes; probes for medical use and medical diagnostic purposes; medical heartbeat measuring apparatus for use in bioassay techniques; replacement parts and fittings for the aforesaid goods; measuring apparatus and instruments, namely, heartbeat measuring apparatus. Scientific and technological services, namely, research and design in the field of pharmaceutics, tissue engineering and regenerative medicine; analysis of industrial fluids and scientific research services; medical and scientific research; pharmaceutical research and development, and clinical testing, namely, conducting clinical trials for others in the field of pharmaceutical preparations, drugs, cell therapy, gene therapy and cancer therapy; development of probes, reagents and bioassays for industrial and scientific purposes; advisory, consultancy and information services relating to the provision of the aforesaid.
An apparatus is provided. The apparatus can include a housing having a first end and a second end. The first end can include a sample inlet and a gas inlet. The second end can include a sample outlet and a gas outlet. The apparatus can also include a sample delivery passage extending within the housing and fluidically coupling the sample inlet to the sample outlet. The apparatus can also include a gas delivery passage extending within the housing and fluidically coupling the gas inlet to the gas outlet. Systems and methods including the apparatus are also provided herein.
A61M 5/30 - Syringes for injection by jet action, without needle, e.g. for use with replaceable ampoules or cartridges
B05B 1/02 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
An apparatus is provided. The apparatus can include a housing having a first end and a second end. The first end can include a sample inlet and a gas inlet. The second end can include a sample outlet and a gas outlet. The apparatus can also include a sample delivery passage extending within the housing and fluidically coupling the sample inlet to the sample outlet. The apparatus can also include a gas delivery passage extending within the housing and fluidically coupling the gas inlet to the gas outlet. Systems and methods including the apparatus are also provided herein.
A61M 5/30 - Syringes for injection by jet action, without needle, e.g. for use with replaceable ampoules or cartridges
B05B 1/02 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner. Related apparatus, systems, techniques, articles and compositions are also described.
The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner. Related apparatus, systems, techniques, articles and compositions are also described.
The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to mammalian cells.
The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to mammalian cells.
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
A61P 43/00 - Drugs for specific purposes, not provided for in groups
B05B 1/02 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops
B05B 7/24 - Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas with means, e.g. a container, for supplying liquid or other fluent material to a discharge device
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
The invention relates to methods of engineering cells (e.g., dendritic cells (DCs)) for vaccinations (e.g., COVID-19) using ethanol-based transient cell membrane permeabilization. Related methods, compositions, apparatus, systems, and articles as described and/or illustrated herein.
A61K 35/15 - Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cellsMyeloid precursor cellsAntigen-presenting cells, e.g. dendritic cells
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C07K 14/165 - Coronaviridae, e.g. avian infectious bronchitis virus
The current subject matter provides a cell engineering platform of a diagnostic and clinical use scale for vector-free and/or viral delivery of payload/cargo compounds and compositions into non-adherent cells. The platform achieves delivery to a large number of cells quickly in a closed system. Related apparatus, systems, techniques, articles and compositions are also described.
The current subject matter provides a cell engineering platform of a diagnostic and clinical use scale for vector-free and/or viral delivery of payload/cargo compounds and compositions into non-adherent cells. The platform achieves delivery to a large number of cells quickly in a closed system. Related apparatus, systems, techniques, articles and compositions are also described.
The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells, e.g. T cells.
The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells, e.g. T cells.
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
C12N 15/89 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
A61K 31/7115 - Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
B05B 7/24 - Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas with means, e.g. a container, for supplying liquid or other fluent material to a discharge device
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
B05B 1/02 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops
25.
ENGINEERING OF IMMUNE CELLS FOR EX VIVO CELL THERAPY APPLICATIONS
The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells, e.g. T cells.
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
C12N 15/89 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
A61K 31/7115 - Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
B05B 1/02 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops
B05B 7/24 - Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas with means, e.g. a container, for supplying liquid or other fluent material to a discharge device
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
C12N 15/89 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
A61K 31/7115 - Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
B05B 7/24 - Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas with means, e.g. a container, for supplying liquid or other fluent material to a discharge device
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.
An apparatus includes a catheter, and an electrode and methods of delivering molecules to eukaryotic cells using such an apparatus. The catheter defines a fluidic channel and has a distal opening. The electrode is within the fluidic channel and is spaced a distance from the distal opening of the catheter. The catheter is arranged to prevent direct contact between any electrode of the apparatus and tissue. Related apparatus, systems, techniques and articles are also described.
B05B 5/00 - Electrostatic spraying apparatusSpraying apparatus with means for charging the spray electricallyApparatus for spraying liquids or other fluent materials by other electric means
B05B 5/16 - Arrangements for supplying liquids or other fluent material
A61M 25/06 - Body-piercing guide needles or the like
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Stem cells for research purposes Pharmaceutical preparations for the treatment of immune-oncological disorders, cancer, diabetes, respiratory, heart, neurological and auto immune disorders; stem cells for medical purposes; living cells for medical use Scientific apparatus and instruments, namely, scientific apparatus and instruments for the transfer of biological molecules into living cells, namely, a transfection system comprised of a preinstalled software controller for operating the transfection system, transfection chamber, tubes, filters, connectors, bags, andreagents; replacement parts and fittings for the aforesaid goods Surgical apparatus and instruments; medical apparatus and instruments for delivery of macro molecules to the mouth, nose, airway, lungs, namely, leukapheresis,plasmapheresis and extracorporeal photopheresis machines; replacement parts andfittings for the aforesaid goods Scientific and technological services, namely, research and engineering processdesign services in the field of pharmaceuticals, tissue engineering and regenerative medicine; analysis of industrial fluids and scientific research services; medical and scientific research; pharmaceutical research and development; and clinical testing, namely, conducting clinical trials for others in the field of pharmaceutical preparations, drugs, cell therapy, gene therapy and cancer therapy;development of probes, reagents and bioassays for industrial, clinical, and scientific purposes; advisory, consultancy and information services relating to theprovision of the aforesaid
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Stem cells for research purposes. Pharmaceutical preparations for the treatment of immune-oncological disorders, cancer, diabetes, respiratory, heart, neurological and auto immune disorders; stem cells for medical purposes; living cells for medical use. Scientific apparatus and instruments, namely, scientific apparatus and instruments for the transfer of biological molecules into living cells; measuring apparatus and instruments, namely, pressure measuring apparatus; probes for scientific purposes, namely, ultrasound probes, not for medical use; bioassay apparatus, namely, pressure measuring apparatus for use in bioassay techniques other than medical; replacement parts and fittings for the aforesaid goods. Surgical apparatus and instruments; medical apparatus and instruments for delivery of macro molecules to the mouth, nose, airway, lungs; veterinary apparatus and instruments, namely, catheter for veterinary purposes; medical and surgical probes; probes for medical use and medical diagnostic purposes; medical heartbeat measuring apparatus for use in bioassay techniques; replacement parts and fittings for the aforesaid goods; measuring apparatus and instruments, namely, heartbeat measuring apparatus. Scientific and technological services, namely, research and design in the field of pharmaceutics, tissue engineering and regenerative medicine; analysis of industrial fluids and scientific research services; medical and scientific research; pharmaceutical research and development, and clinical testing, namely, conducting clinical trials for others in the field of pharmaceutical preparations, drugs, cell therapy, gene therapy and cancer therapy; development of probes, reagents and bioassays for industrial and scientific purposes; advisory, consultancy and information services relating to the provision of the aforesaid.
34.
Vector-free intracellular delivery by reversible permeabilization
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
The current subject matter provides a cell engineering platform of a diagnostic and clinical use scale for vector -free and/or viral delivery of payload/cargo compounds and compositions into non-adherent cells, lire platform achieves delivery to a large number of cells quickly in a closed system. Related apparatus, systems, techniques, articles and compositions are also described.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
stem cells for research purposes Pharmaceutical preparations for the treatment of cancer, autoimmune disease, rare diseases, viruses, and for use in cell transplantation and engineered cells; stem cells for medical purposes; living cells for medical use Scientific apparatus and instruments, namely, scientific apparatus and instruments for the transfer of biological molecules into living cells for the development of cell and gene therapy products; probes for scientific purposes; bioassay apparatus including immune panels; measuring apparatus and instruments, namely, ex-vivo assays for characterization of engineering cells or viruses, other than medical; parts and fittings for the aforesaid goods Surgical apparatus and instruments, medical apparatus and instruments for the modification of cells for near-patient use; medical apparatus and instruments for the modification of cells for near-patient use; measuring apparatus and instruments for bioassay techniques for medical purposes; parts and fittings for the aforesaid goods Scientific and technological services, namely, research and design in the field of process development services for cell and gene therapy products; industrial analysis and research services in the field of cell and gene therapy, and drug delivery; medical, scientific and pharmaceutical research and development, and clinical testing, in the field of pharmaceutical preparations, drugs, cell therapy, gene therapy and cancer therapy; development of probes, reagents and bioassays for industrial and scientific purposes; advisory, consultancy and information services in the fields of biotechnology, pharmaceutical research, laboratory testing, pharmacogenetics, clinical trials, development and genetic science
37.
Vector-free delivery of gene editing proteins and compositions to cells and tissues
The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.
Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.
An apparatus includes a catheter, and an electrode and methods of delivering molecules to eukaryotic cells using such an apparatus. The catheter defines a fluidic channel and has a distal opening. The electrode is within the fluidic channel and is spaced a distance from the distal opening of the catheter. The catheter is arranged to prevent direct contact between any electrode of the apparatus and tissue. Related apparatus, systems, techniques and articles are also described.
B05B 5/00 - Electrostatic spraying apparatusSpraying apparatus with means for charging the spray electricallyApparatus for spraying liquids or other fluent materials by other electric means
Systems, devices, and methods for electro spinning are provided. For example, a system includes a collector including load sensors attached thereto, the collector configured to receive an extruded polymer; and an electro spinning melt head assembly positioned above the collector and configured to extrude the polymer. The electro spinning melt head assembly and/or the collector is configured to move. The melt head assembly includes a syringe assembly and at least one heating element configured to supply heat to the syringe assembly. The syringe assembly includes: a syringe including a passage extending from a proximal end, the passage configured to receive the polymer, and a nozzle configured to allow polymer to pass therethrough.
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
A61K 31/00 - Medicinal preparations containing organic active ingredients
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
C12M 3/02 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
42.
VECTOR-FREE INTRACELLULAR DELIVERY BY REVERSIBLE PERMEABILISATION
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
C12M 3/02 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
A61K 31/00 - Medicinal preparations containing organic active ingredients
The current subject matter includes an adaptive electrospray device that creates consistent output when operating in atmosphere (e.g., not a vacuum). For example, the current subject matter includes an adaptive system that can monitor two current reference points (at the emitter and counter-electrode, respectively), determine a change in emitter current that will account for the parasitic losses, and adjust the emitter current accordingly. In addition, the current subject matter includes a high- throughput adaptive electrospray device having an array of emitters that rapidly switches the electrical potential of different emitters in an array on and off at a predetermined sequence that mitigates or eliminates interference from neighboring emitters. Related apparatus, systems, techniques and articles are also described.
B05B 1/14 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with multiple outlet openingsNozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with strainers in or outside the outlet opening
B05B 12/08 - Arrangements for controlling deliveryArrangements for controlling the spray area responsive to condition of liquid or other fluent material discharged, of ambient medium or of target
B05B 12/18 - Arrangements for controlling deliveryArrangements for controlling the spray area for controlling the spray area using fluids, e.g. gas streams
B05B 5/025 - Discharge apparatus, e.g. electrostatic spray guns
B05B 1/26 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with means for mechanically breaking-up or deflecting the jet after discharge, e.g. with fixed deflectorsBreaking-up the discharged liquid or other fluent material by impinging jets
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
C12N 15/89 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
A61K 31/7115 - Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
B05B 7/24 - Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas with means, e.g. a container, for supplying liquid or other fluent material to a discharge device
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
B05B 1/02 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means designed to produce a jet, spray, or other discharge of particular shape or nature, e.g. in single drops
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Pharmaceutical preparations; stem cells; living cells for medical use. Scientific apparatus and instruments, including scientific apparatus and instruments for the transfer of biological molecules into living cells; measuring apparatus and instruments; probes for scientific purposes; bioassay apparatus; measuring apparatus for use in bioassay techniques (other than medical); parts and fittings for the aforesaid goods in class 9. Surgical, medical, and veterinary apparatus and instruments; medical and surgical probes; probes for medical use and medical diagnostic purposes; measuring apparatus and instruments for use in bioassay techniques (medical); parts and fittings for the aforesaid included in class 10. Scientific and technological services and research and design relating thereto; industrial analysis and research services; medical, scientific and pharmaceutical research and development, and clinical testing, in the field of pharmaceutical preparations, drugs, cell therapy, gene therapy and cancer therapy; development of probes, reagents and bioassays for industrial and scientific purposes; advisory, consultancy and information services relating to the provision of the aforesaid.
46.
VECTOR-FREE DELIVERY OF GENE EDITING PROTEINS AND COMPOSITIONS TO CELLS AND TISSUES
The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, systems, techniques, compositions, and articles are also described.
The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, systems, techniques, compositions, and articles are also described.
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Pharmaceutical preparations; stem cells; living cells for medical use. Scientific apparatus and instruments, including scientific apparatus and instruments for the transfer of biological molecules into living cells; measuring apparatus and instruments; probes for scientific purposes; bioassay apparatus; measuring apparatus for use in bioassay techniques (other than medical); parts and fittings for the aforesaid goods in class 9. Surgical, medical, and veterinary apparatus and instruments; medical and surgical probes; probes for medical use and medical diagnostic purposes; measuring apparatus and instruments for use in bioassay techniques (medical); parts and fittings for the aforesaid included in class 10. Scientific and technological services and research and design relating thereto; industrial analysis and research services; medical, scientific and pharmaceutical research and development, and clinical testing, in the field of pharmaceutical preparations, drugs, cell therapy, gene therapy and cancer therapy; development of probes, reagents and bioassays for industrial and scientific purposes; advisory, consultancy and information services relating to the provision of the aforesaid.
